Dry Diameter Research Articles

Iota-Carrageenan/Chitosan Nanoparticles via Coacervation: Achieving Stability for Tiny Particles

This study investigated the influence of parameters such as pH condition, polyelectrolyte concentration, polymer ratio, and order of addition of the commercial polyelectrolytes chitosan and iota-carrageenan (ι-carrageenan) on the formation of polymeric nanoparticles in suspension (coacervates). A preliminary purification step of the polymers was essential for obtaining stable nanoparticles with small sizes as impurities, particularly metal ions that interfere with complexation, are removed by dialysis. Microparticles (13.5 μm in dry diameter) are obtained when aliquots of chitosan solution are poured into the ι-carrageenan solution. In general, an excess of chitosan results in the formation of agglomerated particles. The addition of an aliquot of ι-carrageenan solution (30 mL at 0.6 mg/mL and pH 4.0) to the chitosan solution (6.0 mL at 0.3 mg/mL and pH 4.0) leads to dispersed nanoparticles with a hydrodynamic radius of 278 ± 5 nm, a zeta potential of −31 ± 3 mV, and an average dry diameter of 45 ± 11 nm. The hydrodynamic radius increases as the pH rises. The partial deprotonation of ι-carrageenan chains enhances the interaction with water molecules, causing the particles to swell. These findings contribute to the fundamental understanding of polyelectrolyte complexation processes in aqueous suspension and provide insights for developing stable nanomaterials for potential practical applications.

Alternative substrates and shading in the initial development of Croton lechleri seedlings

Croton lechleri (dragon's blood) is a forest species used by traditional Amazonian communities for medicinal purposes, with potential for use in degraded area recovery and as a source of additional income for some communities. However, silvicultural studies on this species are scarce. This study aimed to evaluate the influence of substrates and shading levels on seedling emergence and initial development of C. lechleri seedlings. A completely randomized statistical design with four replications was used, arranged in a 7 x 5 factorial setup (substrates and shading levels). The variables analyzed were seedling emergence, emergence speed index, relative emergence frequency, root and shoot length, root and shoot dry mass, stem diameter, and leaf count. Alternative substrates composed of carbonized rice husk and commercial substrate at all shading levels studied favored the emergence and emergence speed index of C. lechleri seedlings. In the 80% shade environment, the substrate consisting of forest topsoil + aged chicken manure (3:1) proved suitable for post-emergence seedling development of C. lechleri.

Bubble-driven heater dynamics in saturated pool boiling

Exploring energy within boiling system is becoming an effective way to enhance heat transfer nowadays. Novel bubble-driven heater has great potential to achieve it. However, heater dynamics in this technique are crucial but unclear in previous studies. Here, boiling on a movable suspended heater with the same density of liquid was simulated to investigate heater dynamics and corresponding mechanisms in detail. Smaller departure diameter and higher frequency were found on movable heater. Periodical up-down motion was observed and the upward movement only occurred at high heat fluxes. Up-down motion contributed to small dry spot diameter and rapid cold liquid supply. The mechanism of different motion over entire bubble cycle was investigated for the first time. Heater moved downwards due to evaporation momentum force (Fe) and add mass force (Fa) during the initial bubble growth stage with expanding triple line, moved upwards due to surface tension force (Fs) during bubble growth period with nearly constant triple line, and it moved downwards again due to Fa when bubble departed. Fa and Fe which impeded bubble departure before were found benefit departure by induced movable heater. Heater manipulation mechanism was found and our study would benefit the design of smart and automatic heater.

Dickson Quality Index of Cocoa Genotypes Under Water Deficit

The aim of this study was to identify patterns of morphological adjustments associated with the Dickson Quality Index (DQI) in Theobroma cacao L. genotypes subjected to water deficit (WD), as a criterion for the pre-selection of drought-tolerant genotypes. Rooted cuttings from seven genotypes were subjected to water deficit (WD). The data from the growth analysis and DQI were subjected to analysis of variance, tests of means, and multivariate analysis. A high correlation was identified between IQD and the variables root dry mass (RDM), leaf dry mass (LDM), stem diameter (SD), and total dry mass (TDM) independently for each genotype; these correlations are more evident in genotypes CP-49, PS-1319, and Cepec-2002. The multivariate analysis divided the genotypes into two major groups: one consisting of the Ipiranga-01, CCN-51, SJ-02, and PH-16 genotypes, and the other comprising the CP-49, Cepec-2002, and PS-1319 genotypes. By correlating the results of the growth analysis with DQI, we were able to identify genotypes CP-49, PS-1319, and Cepec-2002 as tolerant; Ipiranga-01 and CCN-51 as moderately tolerant; and SJ-02 and PH-16 as poorly tolerant to WD. However, it is important that other fields of science are considered to provide greater insights into adaptation to drought.

PHENOTYPIC DIVERSITY OF SWEET POTATO (IPOMOEA BATATAS[L.]LAM.) GERMPLASMUSING QUANTITATIVE TRAITS: A PRE- BREEDING STUDY IN TOGO

Objective: Sweet potato is a root and tuber crop which contribute to food security in Togo. However, little scientific data exists on the genetic variability of cultivars grown in Togo, although this information is a prerequisite for development of new varieties. The objective of this study was to explore the phenotypic diversity within Togos sweet potato cultivars. Materials and Methods: The germplasm is composed of sixty five (65) cultivars from Togo and sixteen (16) exotic varieties introduced from Burkina Faso breeding unit. The experiment was laid out in a 9 x 9 lattice square design with three replicates. In total, sixteen (16) quantitative traits were evaluated using the sweet potato ontology as described by CIP. Results:Descriptive statistics, ANOVA and PCA revealed high variability for traits such as root yield, dry matter content, aboveground biomass, stem length, internode length and stem diameter. Cluster analysis performed on the basis of the Euclidean distance between cultivars using Ward method as aggregation criterion has revealed four phenotypic clusters. Clusters I and II are composed of varieties with low root yield (12.95 and 15.87 t.ha-1) and high dry matter content (29.68 and 26.86%). Clusters III and IV are made of varieties exhibiting high aboveground biomass (37.74 and 50.75 t.ha-1), high fresh root yield (16.06 and 20.18 t.ha-1) and low dry matter content (24.62 and 23.84%). The variability observed in this gene bank constitutes a basis for genetic improvement programmes of sweet potato in Togo.

The effect of pinching and leaf defoliation on plant root architecture and bulb characteristics in lilium

In plants, leaves are the most important organs together with roots in terms of plant development. The effects of leaf defoliation and pinching on root architecture and bulb development were determined in lilies 'Vong' cultivar. For this purpose, leaf defoliation was determined as 0, 25%, 50%, 100%, and pinching was performed. Root length (cm), root surface area (cm2), root volume (cm3), root diameter (mm), number of tips, number of forks, and number of crossings in root architecture were measured. The effect of bulb properties was also determined in main bulb diameter (mm), bulb fresh weight (g), bulb dry weight (g), and bulbil diameter (mm). Leaf defoliation was found to affect root growth negatively. As the defoliation rate increased, the root architectural features decreased compared to the control. In contrast to leaf defoliation, the pinching application significantly increased root length (7568 cm), root surface area (9254 cm2), root volume (176 cm3), number of tips (8553), number of forks (40106), and number of crossings (7950). However, it had no effect on root diameter in two applications. In addition, while leaf defoliation decreased bulb characteristics, pinching application significantly increased bulb fresh weight (54.24 g), bulb dry weight (4.43 g), and bulb diameter (19.43 mm). It was also determined that the application that reduced the bulb fresh weight, bulb dry weight and bulb diameter the most was 100% leaf defoliation. As a result, it was found that pinching is the best method to ensure healthy root growth and bulb development, suggesting that it can be recommended for growing lilium bulbs.

Comprehensive phenotyping of SKUAST-K released rice varieties reveals significant role of root traits in drought resilience

The North Western Himalayan region, particularly the Kashmir valley, harbors a rich diversity of rice landraces, yet systematic studies on climate resilience are lacking. The present study was aimed at investigating the root traits and their interplay with shoot morphological and physiological traits under drought stress using seven indica and three japonica rice varieties released by SKUAST-Kashmir and their possible role in adaptive capacity to different environments. Significant variability was observed in root and shoot traits across the varieties. Root traits exhibited a wide phenotypic range, including rooting depth, fresh and dry weight, diameter, length, volume, surface area, and length density. Associations between root and shoot traits were identified, with notable correlations such as shoot weight with root shoot ratio, shoot length with root dry weight, diameter, and surface area, and tiller number with various root traits. Additionally, physiological traits like canopy temperature depression showed significant correlations with root depth and surface area. Principal component analysis (PCA) analysis grouped japonica type varieties together with a notable outlier, Chenab, due to its superior root traits among indica varieties. These findings emphasize the substantial contribution of root traits to productivity and advocate for their integration into varietal development processes. In view of the increasing evidences in crop plants about their role of root traits in defining plant productivity and reproductive fitness, study of root traits could provide valuable insights into the patterns of crop adaptation to diverse areas and growing environments.

Advanced Dehydration Techniques Standardized for Flowers of Gomphrena globosa L.

The present experiment was conducted on standardizing dehydration technique for Gomphrena globose flower by using embedding drying methods in room temperature and hot air oven at 55°C was done. The experiment consisted of 10 treatments with 3 replications designed in completely randomized design. Treatments used were as; T1-Boric acid (room temperature), T2-Silica gel (room temperature), T3-Sand (room temperature), T4-Sawdust (room temperature), T5-Cocopeat (room temperature), T6-Boric acid (hot air oven at 55 °C), T7-Silica gel (hot air oven at 55 °C), T8-Sand (hot air oven at 55 °C), T9-Sawdust (hot air oven at 55 °C), T10-Cocopeat (hot air oven at 55 °C). The most reduction in flower fresh and dry weight, measuring 2.36 and 2.12 g, was observed in Treatment T7 (Silica gel with hot air oven at 55°C), where silica gel was employed as the desiccant. The least reduction in flower fresh and dry diameter, measuring 1.92 and 1.81 g, was observed in Treatment T7 (Silica gel with hot air oven at 55°C), where silica gel was employed as the desiccant. The highest drying duration for gomphrena in T3 (Sand) and T4 (Sawdust) treatments was 234.21 and 210.40 hrs at room temperature. The boric acid and silica gel demonstrated outstanding performance in terms of quality parameters, including texture, color, and overall appearance. Among all treatment, embedding drying with silica with hot air oven dehydration at 55°C was found to be best in term of reduction in weight, diameter and overall colour, appearance and texture of Gomphrena globose flowers.

Hygroscopic growth and activation changed submicron aerosol composition and properties in the North China Plain

Abstract. Aerosol hygroscopic growth and activation under high-relative-humidity (RH) conditions significantly influence the physicochemical properties of submicron aerosols (PM1). However, this process remains poorly characterized due to limited measurements. To address this gap, we deployed an advanced aerosol–fog sampling system that automatically switched between PM1, PM2.5 and total suspended particulate (TSP) inlets at a rural site in the North China Plain in the cold season. The results revealed that aerosol swelling due to water vapor uptake influenced aerosol sampling under high-RH conditions by shifting the cut-off size of impactors. At subsaturated high RH (> 90 %), over 25 % of aerosol mass with dry diameters below 1 µm resided in supermicron ranges, while in supersaturated foggy conditions, more than 70 % of submicron aerosol migrated to supermicron ranges. Hygroscopic growth and activation particularly affected highly hydrophilic inorganic salts, shifting a significant number of submicron sulfate and nitrate particles to supermicron ranges, with 27 %–33 % at 95 % ≤ RH ≤ 99 % and more than 78 % under supersaturated foggy conditions. Moreover, more than 10 % of submicron biomass burning organic aerosols grew beyond 2.5 µm during fog events, while fossil-fuel-related organic aerosol (FFOA) remained dominantly in submicron ranges, suggesting inefficient aqueous conversion of FFOA. The two secondary organic aerosol (SOA) factors (OOA1 and OOA2) behaved differently under supersaturated conditions, with OOA2 exhibiting a higher activated fraction despite a lower oxygen / carbon ratio. A substantial increase in organic nitrate and organosulfur mass concentrations in activated droplets during fog events suggested aqueous conversions and formations of brown carbon with potential radiative impacts. Overall, our study highlights remarkably different cloud and fog processing behaviors between primary and secondary aerosols, which would benefit a better understanding of aerosol–cloud interactions under distinct atmospheric conditions.

Impact of goat manure application on growth of wild watermelon under shade house cultivation

The continuous use of inorganic fertilizers in agriculture poses detrimental effects on soil health and future crop yields. Therefore, alternative sources of plant nutrients that are environmentally friendly and cost-effective for use in crop production, especially with the escalating cost of inorganic fertilizers, are necessary. A shade-house study was designed to determine the effect of different quantities of goat manure applications on growth attributes and yield of wild watermelon (Citrullus lanatus subsp.). A randomized complete block design (RCBD) was used to arrange the treatments, with fourteen (14) replications (n = 84). Six (6) treatments consisting of various quantities (v/v) of river sand, Hutton soil, and goat manure (T0 -1:3:0, T1 -1:3:1, T2 -1:3:2, T3 -1:3:3, T4 -1:3:4, and T5 -1:3:3) were used, with T0 -1:3:0 constituting the control. Treatments had a highly significant (P ≤ 0.01) effect on dry shoot mass (65%), fruit weight (75%), and fruit diameter (75%) except for the dry root mass (44%), vine length (47%), chlorophyll (47%), number of fruit (27%) and stem diameter (22%) of wild watermelon plants. Dry shoot mass was the highest (11.14 g) in T4 -1:3:4, and the lowest dry shoot mass (6.57 g) was obtained in T1 -1:3:1 when compared to the control. The fruit fresh weight of the wild watermelon was reduced in all the treatments, when compared to the control. The biggest fruit diameter (66.66 mm) was observed in T4 -1:3:4, and the lowest (14.4 mm) was observed in T5 -1:3:5. In conclusion, T4 -1:3:4 enhanced greater vegetative growth (dry shoot matter) and yield attributes (fruit diameter and weight) of the wild watermelon.

Enhancing Leafy Vegetable Growth and Yield with Goat Urine, Moringa Leaf, and Banana Stem-based Liquid Organic Fertiliser

Pak choy and mustard greens are traditionally grown with many inorganic fertilisers, which can reduce soil fertility when applied frequently. The adoption of organic fertilisers offers a sustainable solution to this challenge. This study investigates the impact of liquid organic fertiliser (LOF) derived from goat urine, Moringa leaves, and banana stems on the growth and yield of pak choy and mustard greens. The research design employed a randomised complete block design with four treatments and ten replications. These treatments included a control group, 100% nitrogen, phosphorus, potassium (NPK, inorganic fertiliser), 100% LOF, and a 50% NPK + 50% LOF blend. The application of LOF, sourced from goat urine, Moringa leaves, and banana stems, demonstrated a significant influence on nearly all plant parameters. Notably, the 100% LOF treatment yielded the highest results for fresh leaf weight (26.46 g), fresh stalk weight (39.64 g), dry leaf weight (4.57 g), stem diameter (48.27 mm), Soil Plant Analysis Development value (SPAD, 40.73 units), plant height (31.59 cm), leaf width (8.45 cm), and leaf length (13.33 cm) for pak choy. Additionally, 100% LOF also produced the highest results for fresh leaf weight (21.34 g), dry root weight (0.31 g), stem diameter (21.53 mm), SPAD value (32.40 units), plant height (31.59 cm), leaf width (10.40 cm), and leaf length (15.65 cm) for mustard greens. This study demonstrates that a blend of goat urine, Moringa leaves, and banana stems can be used as a LOF that can replace inorganic NPK fertilisers in growing pak choy and mustard. It not only addresses issues with soil fertility but also contributes to environmentally friendly farming practices.

Mitigating the detrimental impacts of low- and high-density polyethylene microplastics using a novel microbial consortium on a soil-plant system: Insights and interactions

The accumulation of polyethylene microplastics (PE-MPs) in soil has raised considerable concerns; however, the effects of their persistence and mitigation on agroecosystems have not been explored. This study aimed to assess the detrimental effects of PE-MPs on a soil-plant system and evaluate their mitigation using a novel microbial consortium (MC). We incorporated low-density polyethylene (LDPE) and high-density polyethylene (HDPE) at two different concentrations, along with a control (0 %, 1 %, and 2 % w/w) into the sandy loam soil for a duration of 135 days. The samples were also treated with a novel MC and incubated for 135 days. The MC comprised three bacterial strains (Ralstonia pickettii (MW290933) strain SHAn2, Pseudomonas putida strain ShA, and Lysinibacillus xylanilyticus XDB9 (T) strain S7–10F), and a fungal strain (Aspergillus niger strain F1–16S). Sunflowers were subsequently cultivated, and physiological growth parameters were measured. The results showed that adding 2 % LDPE significantly decreased soil pH by 1.06 units compared to the control. Moreover, adding 2 % HDPE resulted in a more significant decrease in soil electrical conductivity (EC) relative to LDPE and the control. A dose-dependent increase in dissolved organic carbon (DOC) was observed, with the highest DOC found in 2 % LDPE. The addition of higher dosages of LDPE reduced soil bulk density (BD) more than HDPE. The addition of 2 % HDPE increased the water drop penetration time (WDPT) but decreased the mean weight diameter of soil aggregates (MWD) and water-stable aggregates (WSA) compared to LDPE. The results also revealed that higher levels of LDPE enhanced soil basal respiration (BR) and microbial carbon biomass (MBC). The interaction of MC and higher MP percentages considerably reduced soil pH, EC, BD, and WDPT but significantly increased soil DOC, MWD, WSA, BR, and MBC. Regarding plant growth, incorporating 2 % PE-MPs significantly reduced physiological responses of sunflower: chlorophyll content (Chl; −15.2 %), Fv/Fm ratio (-25.3 %), shoot dry weight (ShD; −31.3 %), root dry weight (RD; −40 %), leaf area (LA; −38.4 %), and stem diameter (StemD; −25 %) compared to the control; however, the addition of novel MC considerably reduced and ameliorated the harmful effects of 2 % PE-MPs on the investigated plant growth responses.

Sea Spray Aerosol Over the Remote Oceans Has Low Organic Content

AbstractBiogenic organic compounds in the surface ocean may significantly alter the cloud‐forming ability of sea spray aerosol and thereby affect the amount of solar radiation reaching the ocean surface. Estimates of the organic mass fraction of sea spray vary widely, and some results show a significant dependence on biological activity in the source seawater. We present airborne observations of the organic mass fraction of individual sea spray particles measured using the Particle Analysis by Laser Mass Spectrometry (PALMS) instrument during the Atmospheric Tomography (ATom) mission, which sampled a wide range of latitudes and altitudes over the remote Atlantic and Pacific Oceans across four seasons, from the marine boundary layer to the upper troposphere. The measured sea spray particles of about 0.15–0.7 μm dry diameter showed higher average organic mass fractions at smaller sizes, but values were low overall, with regional integrated submicron means almost always <10%. Atmospheric aging adds organics to sea spray particles, leading to higher mean organic mass fractions (sometimes exceeding 50%) in the free troposphere than in the marine boundary layer. The average submicron sea spray organic mass fractions are on the low end of previously reported values and show weak seasonal variability for most regions. These results imply that recent biological activity in the surface ocean has only weak control over how much organic matter is in nascent submicron sea spray particles over the remote oceans, in contrast to findings from some observational studies and global numerical simulations.

The ability of biostimulants and copper-containing fungicide to protect cotton against chilling stress

BackgroundCotton (Gossypium hirsutum L.), adapted to tropical and subtropical regions of the world, is highly sensitive to low temperatures throughout its life cycle. The objective of this study was to evaluate the mitigating effects of different doses of animal-derived (0.25%, 0.50%, and 1.00% Isabion® ), seaweed-based (0.165%, 0.330%, and 0.660% Proton®) biostimulants, as well as a copper (Cu)-containing fungicide application, on cotton cultivar Lazer seedlings at the four true leaves (V4) stage. The plants were exposed to a low temperature of 5 °C for 48 h, and the changes in morphological (seedling fresh and dry weight, plant height, and stem diameter) and physiological parameters (leaf temperature, chlorophyll content, relative water content, electrolyte leakage, and relative injury) were examined.ResultsThe results revealed that chilling stress reduced plant growth, while biostimulants helped protect the plants and overcome the adverse effects of chilling. Under chilling stress, there was a considerable reduction in seedling fresh weight (SFW), seedling dry weight (SDW), plant height (PH), stem diameter (SD), leaf temperature (LT), and relative water content (RWC). Cotton seedlings treated with the animal-derived biostimulants showed significantly enhanced SFW, SDW, PH, SD, LT, chlorophyll content (Chl), electrolyte leakage (EL), and relative injury (RI), although there were no positive changes in RWC. No significant differences in the morphological traits were observed among the doses of seaweed biostimulants. For SDW, PH, EL, and RI, the best results were obtained with the application of a fungicide containing copper.ConclusionThese results show the efficiency of the biostimulant and fungicide treatments in mitigating low-temperature stress in cotton seedlings. Applying a copper-containing fungicide to cotton seedlings helped to counteract the negative effects of low-temperature stress and to protect the plants from damage by maintaining electrolyte balance. Among the biostimulant applications, all levels of animal-derived biostimulant applications, as well as the 0.660% level of the seaweed-derived biostimulant, led to increased tolerance of cotton plants to chilling stress.

Controlled Delivery of Paclitaxel via Stable Synthetic Protein Nanoparticles.

Despite decades of intense research, glioma remains a disease for which no adequate clinical treatment exists. Given the ongoing therapeutic failures of conventional treatment approaches, nanomedicine may offer alternative options because it can increase the bioavailability of drugs and alter their pharmacokinetics. Here, a new type of synthetic protein nanoparticles (SPNPs) is reported that allow for effective loading and controlled release of the potent cancer drug, paclitaxel (PTX) - a drug that so far has been unsuccessful in glioma treatment due to hydrophobicity, low solubility, and associated delivery challenges. SPNPs are prepared by electrohydrodynamic (EHD) jetting of dilute solutions of PTX-loaded albumin made by high-pressure homogenization. After EHD jetting, PTX SPNPs possess a dry diameter of 165 ± 44 nm, hydrated diameter of 297 ± 102 nm, and a zeta potential of -19 ± 8 mV in water. For the SPNP formulation with a total PTX loading of 9.4%, the loading efficiency is 94%, and controlled release of PTX is observed over two weeks (6% burst release). PTX SPNPs are more potent (68% lethality) than free PTX (45% lethality using 0.2% dimethyl sulfoxide). PTX SPNPs in combination with IR show a significant survival benefit in glioma-bearing mouse models, avoid adverse liver toxicity, and maintain a normal brain architecture. Immunohistochemistry reveals a dramatic tumor size reduction including 40% long-term survivors without discernible signs of tumor. Using flexibly engineered SPNPs, this work outlines an efficient strategy for the delivery of hydrophobic drugs that are otherwise notoriously hard to deliver.

Residual plastic film decreases crop yield and water use efficiency through direct negative effects on soil physicochemical properties and root growth

Film mulching has been extensively used to improve agricultural production in arid regions of China. However, without sufficient mulch film recovery, large amounts of residual film accumulated in the farmland, which would affect crop yield and water use efficiency (WUE). In order to comprehensively analyze the effects of residual film on crop yield and WUE, and clarify its influencing mechanism, present study adopted a meta-analysis to systematically evaluate the impacts of residual film on soil physicochemical properties, crop root growth, yield, and WUE. The results showed that residual film significantly increased soil bulk density and the soil moisture content in 0–20 cm soil layer, but decreased soil porosity, soil organic matter, soil total nitrogen content, and soil moisture content in >20 cm soil layer, especially when residual film amount was >400 kg ha−1. Residual film significantly reduced crop root dry weight, root length, root diameter, root volume and root surface area. Generally, crop yield and WUE decreased with the increase of residual film amount; and crop yield was reduced by about 14.00 % when the residual film amount increased by 1000 kg ha−1. In average, crop yield and WUE under film residual condition were significantly decreased by 13.46 % and 9.21 %, respectively. The negative effects of residual film on root growth, yield and WUE were greater for cash crops (cotton, tomato and potato) than for cereal crops (wheat, maize). The structural equation model indicated that residual film generated indirect negative effects on crop yield and WUE by directly affecting soil physicochemical properties and crop root growth, with the standard path coefficients of −0.302 and − 0.217, respectively. The results would provide a theoretical basis for reducing residual film pollution on farmland and promoting the green and sustainable development of agriculture.

Aggregate stability and carbon and N dymamics in macroaggregate size fractions with different soil texture

Soil nutrient cycling, the distribution of soil aggregates, and their stability are directly influenced by soil texture. Different sizes of soil aggregates provide microhabitats for microorganisms and therefore influence soil carbon (C) and nitrogen (N) mineralization. The purpose of the present study was to assess the aggregate stability and dynamics of carbon and nitrogen in macroaggregate size fractions (1-8 mm) with different clay content from meadow soils. Surface soil samples (0-15 cm) were collected from 4- to 5-year-old forage crops. Four macroaggregate size classes were isolated by dry sieving and analyzed for their mass proportions: fine macroaggregates (FM) (less than 1 mm), medium-fine macroaggregates (MFM) (1-2 mm), medium-coarse macroaggregates (MCM) (2-4 mm), and large-coarse macroaggregates (LCM) (4-8 mm). The dry mean weight diameter (MWD), organic carbon (OC), total nitrogen (TN), carbon and nitrogen of microbial biomass (C-MB, N-MB) were determined. CO2 emission and net nitrogen mineralized (NM) were measured after 14 weeks of incubation. The amounts of FM were significantly lower than those of intermediate macroaggregates (MCM and MFM) and decreased markedly with increasing clay content within soil macroaggregates. In general, the amounts of macroaggregate size fractions were lowest in soils with high clay content. MWD exhibited a significant correlation with particle size distribution, OC, and MB-C. OC, TN, MB-C, and MB-N contents within macroaggregates increased with decreasing macroaggregate size and increasing clay content of macroaggregate fractions. The CO2 emission and NM content increased with increasing macroaggregate size, indicating higher organic C and N mineralization activity in larger macroaggregates. Mineralization of OC was lowest in macroaggregate fractions with the highest clay content. We conclude that clay content can increase the protection of microbial biomass in meadow soils. Small macroaggregates tend to contain more recalcitrant organic matter compared to larger macroaggregates.

Comparing vegetative growth patterns of cultivated (Daucus carota L. subsp. sativus) and wild carrots (Daucus carota L. subsp. carota) to eliminate genetic contamination from weed to crop

Wild carrot is a problematic weed that can threaten the genetic purity of cultivated carrots by hybridization. Wild carrots must be controlled before flowering to avoid the undesirable crossing with cultivated carrots. Understanding wild carrot's vegetative growth pattern helps formulate sustainable weed management practices. However, little is known about the vegetative growth patterns of wild and cultivated carrots. A pot experiment was carried out to compare and model the vegetative growth pattern of different morphological traits in both wild and cultivated carrots. This study was executed in a glasshouse located in Palmerston North, New Zealand. A factorial randomized complete block design (RCBD) with two factors and four replications was used. The first factor was assigned to the carrot genotype (cultivated and wild) and the second factor to length of juvenile stages (12-weeks, 8-weeks, and 4-weeks). Plant height, leaf number, shoot fresh and dry weight, root fresh and dry weight, root diameter and root length were measured. Data were analyzed using analysis of variance (ANOVA), principal component analysis (PCA), correlation, and regression analysis. At the 8-week juvenile stage (9–11 leaves stage), wild carrot's shoot and root characteristics exhibited rapid growth. Correlation analysis indicated positive and significant (p < 0.05) correlations between above and below-ground morphological traits. PCA showed that morphological characteristics, except plant height, can be used to distinguish wild and cultivated carrots. To predict the vegetative growth pattern of most of the morphological traits of wild and cultivated carrots, power regression models were selected based on higher R2 and adj-R2 values and lower values of RMSE, AIC and BIC. The study showed wild carrots grew more quickly than cultivated carrots during the vegetative phase. It is recommended that appropriate weed management practices, such as hoeing, tilling, hand pulling, or herbicide spraying, be implemented before wild carrot leaf stages 9–11.

Effect of Planting Media and Cultivation Method in the Offshoots Growth of Date Palm Phoenix Dactylifera L. Hillawi cv.

Objective: The study aimed to explore the effects of different planting medium on the growth of date palm Hillawi cv. offshoots. Methods: The experiment included two main factors: the first factor was planting media, consisting of three treatments: field soil, zeolite + field soil (in a 2:1 ratio), and zeolite + sand (in a 2:1 ratio). The second factor was cultivation method, comprising three methods: traditional planting, container planting (using pots), and planting in lined holes with a layer of agricultural polyethylene (nylon). Results: Planting medium significantly influenced the growth of the offshoots. Specifically, planting media with zeolite and sand exhibited significant improvements in leaf length, dry matter content, leaf area, root count, root length, and diameter compared to the control group. Conversely, the zeolite-field soil combination exhibited a marked increase in moisture content. Additionally, electrical conductivity (EC) within the planting media was notably higher in the control condition featuring only field soil. The study also found that the chosen cultivation method significantly influenced the growth of the offshoots. The lined holes method displayed significant advantages in leaf length, dry matter content, leaf area, root count, root length, root diameter, and moisture content within the planting media, compared to the traditional approach. Conversely, the traditional method resulted in higher EC within the planting media. Conclusions: The study reveals significant effects of cultivation medium, method, and their interaction on various growth parameters of date palm offshoots. This study recommends the lined-hole planting method and zeolite-infused medium for enhanced offshoot growth.

Use of processed grape pomace and whey bio ferment to improve the agronomic performance of radish (Raphanus sativus L.) in arid soils

Agro-industrial wastes representing a significant problem can be revalued as biofertilizers. The present paper aims to determine the effect of processed grapevine pomace (PGP) and whey bio-ferment (WB) on radish cultivation under conditions of arid zone soil. A 3x3 factorial arrangement of completely randomized design was used, with three levels of PGP (0, 1.25, and 2.50 g kg-1 soil applied in total dose before planting) and three levels of WB (0, 50, and 100 mL L-1 applied in irrigation water). Radish leaves were evaluated for length and chlorophyll; root dry matter content, length, diameter, weight, and total soluble solids (TSS) were measured; organic matter (OM), N, P, K, pH, electrical conductivity (EC) and cation exchange capacity (CEC) was determined in the soil. Specific differences in means were determined by the LSD-Fisher method applied after analysis of variance (ANOVA); the significance of differences was defined at P&lt;0.05. The most remarkable result to emerge from the data is that using PGP and WB improved soil attributes and promoted crop development. Specifically, the higher the PGP dose, the greater the benefits; in the case of WB, the dose of 50 mL L-1 showed the best results.