Availability Of Drugs Research Articles

Drug supply management at first-level public health facilities: Case of Pyay District, Myanmar.

First-level public health facilities (PHFs) serve as primary providers of essential medicines, necessitating critical attention to drug availability and quality assurance. This study aimed to examine the status of functional areas within the drug supply chain management framework and assess the overall capability maturity at first-level PHFs. The cross-sectional study was conducted among 183 drug store sites from six townships of Pyay District. Only situational analysis was exercised to determine the existing situations. The overall capability maturity was determined according to the definitions of levels of the Capability Maturity Module Tool. 58.47% lacked formal drug supply management training, with 23.5% not undergoing performance reviews. Drug forecasting predominantly relied on a pen-paper system (91.6%) and factors like patient load (87.39%), drug consumption (85.71%), and disease prevalence (64.71%). Store site analysis revealed that 65.03% exhibited marginal capability, lacking standardized drugstores and employing unstandardized procedures. Storage practices varied, with 48.69% storing drugs conveniently and others categorizing them by drug type (32.79%) or using the first-expired-first-out system (40.98%). Approximately 42.69% reported having expired drugs. Concerning transportation costs, 37.16% incurred expenses exceeding 20,000 Kyats per time, with management staff often covering the costs. Waste management methods included burial pits (49.18%), incineration (62.84%), and sharp pits (55.19%). A majority (78.14%) used safety boxes, and 57.38% implemented a color-coded system for waste bins. The logistics management information system was entirely paper-based (100%). On average, assessments of drug quality conditions and physical damages scored 46.51% and 48.20%, respectively. The overall supply chain maturity at first-level public health facilities is at a marginal capability level (36.35%). While some basic drug supply chain management procedures were in place, they were not consistently followed, and many systems remain manual. The findings underscored significant inconsistencies in the management functions of supplied drugs, with poor adherence to Standard Operating Procedure guidelines.

PH/Hypoxia dual-stimuli responsive COF nanocarriers with reversible charge conversions for the accurate tumor-targeting and enhanced anticancer drug delivery

Nanoparticles-based drug delivery systems show great promise for cancer therapy due to the advantages of crossing physiological barrier, high drug availability and good stability. However, deep tumor penetration, long blood circulation, and rapid drug release remain intractable problems for nanocarriers. In this work, we report a tumor microenvironment-sensitive nanodrug delivery system containing both acidic pH and hypoxia response sites to achieve enhanced drug permeability and on-demand release for precise cancer therapy. The covalent organic frameworks (COFs) with introduced azobenzene (azo) groups were used as employed as nanocarrier, and modified by pH-sensitive sulfamide-based zwitterionic polymers (PBA), followed by loading with the chemotherapeutic drug doxorubicin (DOX) to obtain COF-PBA@DOX. Copolymer PBA achieved charge conversions in tumor acidic environment, resulting in a positive charge on the nanocarrier surfaces, thus facilitating the augmented tumor accumulation. Meanwhile, azo which was formed the COF carrier, could be reduced to amines by the highly expressed azoreductase under tumor hypoxia conditions, resulting in nanoparticle dissociation and the drug-controlled release at the solid tumor sites. The fabricated dual-responsive nanomedicine, based on the surface charge-reversal of zwitterion in the acidic environment of tumor, can realize the accumulation and targeted drug release at the tumor site, which has great potential as a nanocarrier in tumor therapy.

Analysis of Public Health Care Safeguard Measures in India

Public health is a branch of science that focuses on preventing disease and injuries in large populations by doing research, educating the public, and creating policies that promote health in all communities. Each person may define public health in a different way. In order to address global health issues, address community-wide health issues, and influence global health policies at both local and global levels, public health is a field of study that encompasses a variety of academic fields, including basic science, commerce, the arts, education, technology, engineering, computer science, pharmacy, and bioscience. Excluding the effects of non-intellectual property barriers like distribution, infrastructure, quality, and medical facilities, the goal of this study is to examine the public health barriers to intellectual property that affect medical access. This study aims to investigate how patent law protects drug accessibility while taking drug prices into consideration.

Public Health and Patent Law to Access Medicine in India

India's 1970 decision to include a process patent regime in its patent legislation is regarded as a reformative step that was a well-considered reaction to internal issues including the nation's then-current socioeconomic realities. The primary goal of this research project has been to critically analyse the connections among public health, the global intellectual property law, and the availability of necessary medications, with a particular emphasis on India. The study's main focus is the persistent issue of developing and least developed nations' lack of access to reasonably priced medical care in the post-crisis age. This study aims to investigate the inherent barriers to intellectual property that impact medical access, excluding the impact of non-intellectual property impediments such as distribution, infrastructure, quality, and medical facilities. The purpose of this study is to examine how patent law affects drug accessibility while accounting for drug costs.

The Peer Group Effect on Drug Abuse and Academic Performance Among University Student

The drug problem is one of the most complex problems in the world, and hardly any society is devoid of it, whether in developed or developing countries. It threatens students' social, economic, and educational life at all levels. Saudi Arabia like the other countries faces drug spread among the youth. This study aims to assess the role of peer group effect on drug abuse and academic performance among university students in the Hawtat Bani Tamim. The study adopts the questionnaire as a tool for collecting data. The sample size of the study was 121 participants from university students. The study adopted descriptive statistics and inferential statistics. The results of the study found that the prevalence of drug abuse among university students was 8.3. There is a significant relationship between drug abuse and several factors under investigation. Notably, there were notable connections identified between drug abuse and age, the availability of drugs, and parental influence status. Also, the result showed that drug abuse delays graduation and absence from university. Peer group pressure influences drug abuse among students and plays a crucial role in drug abuse among university students. The study recommends providing programs that encourage students to quit drug abuse.

Drug Availability and Quality Under Stress: The Hidden Risks of Supply Chain Disruptions

Drug Availability and Quality Under Stress: The Hidden Risks of Supply Chain Disruptions

Supply-side factors influencing national health insurance uptake, access and use of primary health care: Evidence from the Upper West Region, Ghana

Supply-side factors, such as the availability of health facilities, equipment, practitioners, and drugs, are crucial for improving global healthcare systems. However, systematic evidence of how these factors affect health insurance clients’ access to primary healthcare (PHC) services in Ghana is lacking. This study addresses this gap by examining the impact of these factors on insured individuals' access to PHC services in Wa Municipality. An explanatory sequential mixed-methods design was employed, involving a quantitative survey of 413 insured individuals randomly selected from 40 communities and 47 healthcare facilities. Purposive sampling was used for qualitative interviews with 17 healthcare providers and 20 key insured informants. Quantitative data were analysed using descriptive statistics, correlation, independent t-tests, and binary logistic regression, while qualitative data were thematically analysed to provide insight into the quantitative findings. Result from quantitative data showed that Community-Based Health Planning and Services (CHPS) were more accessible than hospitals, diagnostic centres, and health centres. Proximity to health facilities influenced access to PHC services and National Health Insurance Scheme (NHIS) uptake. Perceived availability and functionality of healthcare equipment also affected NHIS uptake. Qualitatively, health facilities reported a shortage of human resources, such as midwives and nurses, for PHC service delivery. Triangulating quantitative and qualitative results show that healthcare supplies and skilled professionals are essential for NHIS uptake and access to PHC services. This study recommends that Government of Ghana prioritise expanding healthcare facilities, recruiting and retaining skilled practitioners, procuring medical equipment, and equitably distributing essential medications to improve access to essential healthcare services in underserved areas.

New Evidence of the Impact of the National Drug Price Negotiation Policy on the Availability, Utilization, and Cost of Anticancer Medicines in China: An Interrupted Time Series Study.

The increasing global burden of cancer has become a significant challenge for public health. The Chinese government introduced the National Drug Price Negotiation (NDPN) policy with the goal of lowering the prices of innovative drugs and enhancing their accessibility. This study aims to evaluate the impact of the 2021 NDPN policy on the availability, utilization, and cost of anticancer medicines in China. Data was gathered from 1519hospitals between April 2021 and December 2022, with a focus on eight anticancer drugs affected by the 2021 NDPN policy. The availability, Defined Daily Doses (DDDs), and cost per Defined Daily Dose (DDDc) before and after the intervention were evaluated through interrupted time series analysis. The NDPN policy resulted in a substantial 5.10% increase in the availability of anticancer drugs (p < 0.001). Utilization also experienced a significant surge, with an immediate increase of 11,254.36 DDDs (p < 0.001) and a monthly increase of 1208.28 DDDs (p < 0.001) following policy implementation. The DDDc decreased by US$ 111.00 (p < 0.001) immediately after the policy. Disparities in regional drug utilization were evident, with higher usage in the eastern region. The 2021 NDPN policy has notably enhanced the availability and utilization of anticancer medications in China while reducing their cost, in line with the policy's objectives. However, continuous monitoring is essential to ensure sustained access and to tackle regional disparities in drug utilization.

Managing naïve multiple sclerosis patients: how firstline oral medications modify educational time

Multiple sclerosis is a chronic disease requiring a multidisciplinary approach. Multiple sclerosis nurses are involved in all aspects of care, in particular in patients' education about disease-modifying drugs. The availability of oral firstline treatments has had a considerable impact on disease management. The aim of this study was to valuate if the introduction of oral therapies has led to a change in nurses' time spent on education, empowerment and training. A retrospective study was conducted enrolling naïve patients with relapsing-remitting mutliple sclerosis. Working time spent by nurses was compared between patients treated with oral versus injectable drugs. Correlations between time and clinical variables were investigated. A total of 55 patients were enrolled (34 using injectable and 21 using oral medication). For the oral group, less work time (P=0.018) and number of meetings (P=0.004) were needed. Dimethyl fumarate needed a lower number of meetings compared with other drugs. The availability of oral drugs seemed to reduce the amount of working time nurses spent with naïve patients' discussing education and treatment.

Immunomodulatory Effects of Statins in the Treatment and Prophylaxis of Influenza Viruses

Background and aim: Influenza, commonly referred to as "the flu," is an infectious illness caused by influenza viruses (IV). Despite the availability of efficient antiviral drugs, the IV still significantly increases mortality. Antiviral drugs swiftly remove these viruses from pulmonary secretions, suggesting that a prolonged inflammatory response may be to blame for the poor result. Therefore, using immunomodulatory medications looks advantageous. Numerous anti-inflammatory effects are produced by statins. Numerous retrospective studies recommended that statins be considered for IV therapy. This study set out to comprehensively examine the immunomodulatory potential of statins in IV prophylaxis and therapy. Methods: From the beginning until 25 March 2022, a number of electronic databases (Scopus, EMBASE, PubMed, Web of Science, ProQuest, OVID, EBSCO, and CINAHL) were searched for cohort studies and randomized trials assessing the association between outcomes or risk of infections and statin therapy. Data were gathered on the investigated characteristics, measuring statin usage, quality evaluation, and results (set for potential confounders). Results: Finding additional therapeutic agents for influenza prevention and management is crucial since there are uncertain public health issues regarding the clinical efficacy of conventional medications. Pro-inflammatory cytokines are released as a result of influenza. According to the results, by using anti inflammatory medications, severe influenza infections can be prevented along with pleiotropic effects. As a result, statins, which are cardioprotective medications with immunomodulatory and anti-inflammatory properties, may be beneficial for individuals with IV. Conclusion: Based on the positive immunomodulatory effects of statin administration, our systematic review's findings suggested that IVs may be prevented and treated.

Drug Delivery from A Ring Implant Attached to Intraocular Lens: An In-Silico Investigation

Multiple iterations required to design ocular implants, which will last for the desired operational period of months or even years, necessitate the use of in-silico models for ocular drug delivery. In this study, we developed an in-silico model to simulate the flow of Aqueous Humor (AH) and drug delivery from an implant to the Trabecular Meshwork (TM). The implant, attached to the side of the intraocular lens (IOL), and the TM are treated as porous media, with their effects on AH flow accounted for using the Darcy equation. This model accurately predicts the physiological values of Intraocular Pressure (IOP) for both healthy individuals and glaucoma patients, as reported in the literature. Results reveal that the effective diffusivity of the drug within the implant is the critical parameter that can alter the bioavailability time period (BTP) from a few days to months. Intuitively, BTP should increase as effective diffusivity decreases. However, we discovered that with lower levels of initial drug loading, BTP declines when effective diffusivity falls below a specific threshold. Our findings further reveal that, while AH flow has a minimal effect on the drug release profile at the implant site, it significantly impacts drug availability at the TM.

Synergistic effects of herpes oncolytic virus and cyclophosphamide for recurrent malignant glioma: a narrative review.

Gliomas, comprising nearly 80% of brain malignancies, present a formidable challenge with glioblastomas being the most aggressive subtype. Despite multidisciplinary care, including surgery and chemoradiotherapy, the prognosis remains grim, emphasizing the need for innovative treatment strategies. The blood-brain barrier complicates drug access, and the diverse histopathology hinders targeted therapies. Oncolytic herpes viruses (oHSVs), particularly HSV1716, G207, and rQNestin34.5v, show promise in glioma treatment by selectively replicating in tumor cells. Preclinical and clinical studies demonstrate the safety and efficacy of oHSVs, with T-Vec being FDA-approved. However, challenges like viral delivery limitations and antiviral responses persist. The combination of oHSVs and combining cyclophosphamide (CPA) addresses these challenges, demonstrating increased transgene expression and viral activity. The immunosuppressive properties of CPA, particularly in metronomic schedules, enhance oHSV efficacy, supporting the development of this combination for recurrent malignant gliomas. CPA with oHSVs enhances viral oncolysis and extends survival. CPA's immunomodulatory effects, suppressing regulatory T cells, improve oHSV efficiency. While obstacles remain, this synergistic approach offers hope for improved outcomes, necessitating further research and clinical validation.

Mussel-inspired adhesive drug-loaded hydrogels for oral ulcers treatment

Oral aphthous ulcers are common mucosal lesions that cause pain and discomfort. There are diverse biomaterials and drug treatments for oral ulcers used in both research and clinical settings. However, the complex oral environment often results in low adhesion and short drug retention times, which lead to poor drug availability and treatment outcomes. In this study, a mussel-inspired adhesive hydrogel was developed by grafting catechol onto hyaluronic acid (C-HA), and dopamine was added for oxidative pre-polymerization to form modified hyaluronic acid (M-HA), which remarkably increased the adhesion of the hydrogels. Then, M-HA was interpenetrated into the gelatin methacryloyl (GelMA) network. Chlorhexidine gluconate (CHG) was then incorporated into the hydrogel to enhance its availability and therapeutic effect through its sustained-release capability. The GelMA/M-HA hydrogel demonstrated strong adhesion to wet tissues, antibacterial and anti-inflammatory properties, and good biocompatibility. In both rat oral ulcers and infected wounds, the adhesive hydrogel significantly accelerated the healing of the ulcers and infected wounds. These results indicated that this adhesive hydrogel offers a promising new strategy for the treatment of oral ulcers in clinical practice. Statement of significanceOral ulcers are a common and high-incidence mucosal condition that seriously affect people's daily lives, often making it difficult for patients to chew and speak. However, a dynamic oral environment with various types of bacteria influences drug availability and treatment effects in clinical settings. To address this challenge, an adhesive, mussel-inspired, drug-loaded hydrogel was constructed using natural macromolecules (hyaluronic acid and gelatin) with good biocompatibility. Chlorhexidine gluconate (CHG), with its broad-spectrum antibacterial activity, has been incorporated to synergistically promote oral ulcer healing. The splendid adhesion, antibacterial, and therapeutic effects of this hydrogel demonstrated a new strategy for treating oral ulcers.

A study of India’s ‘People’s Medicine Scheme’: Policy recommendations and insights to promote and improve access to generic medicines based on pilot survey in Bengaluru Urban District, India

Access to affordable and quality medicines plays a vital role for achieving Universal Health Coverage and in reducing out-of-pocket expenditures (OOPE) for households especially in developing nations such as India. Pradhan Mantri Bhartiya Janaushadhi Pariyojana (PMBJP), a Government of India initiative, aims to make low-cost and quality generic drugs and surgical equipment accessible to all segments of the population through its dedicated store outlets known as Pradhan Mantri Bhartiya Janaushadhi Kendra (PMBJK). In this study, a pilot survey comprising 20 stores/PMBJKs and 150 citizens was undertaken in the Bengaluru Urban District, India to understand various aspects of the PMBJKs including availability of drugs, stock-outs, accessibility to stores, perception and awareness levels along with challenges faced by store owners and citizens/beneficiaries. Based on the survey results, we capture the availability of drugs for 35 medicines and consumables belonging to 12 therapeutic categories across 20 store outlets. We also provide valuable insights and interdisciplinary recommendations on several facets including adopting technology-based measures for day-to-day functioning of stores, need for in-depth supply chain analysis for ensuring availability of drugs, encouraging prescription of generic medicines, increasing awareness levels in addition to promoting grassroot-level research, surveys and feedback mechanisms. These suggestions are expected to find their utility in policy-making, strengthen the implementation of the PMBJP scheme across Bengaluru and India as well as contribute towards achieving related Sustainable Development Goals.

Choice intention for the national volume-based procurement drug and its associated factors: a cross-sectional study on patients with late-life depression in China

BackgroundThe national volume-based procurement (NVBP) policy has significantly decreased prices and increased the accessibility of NVBP drugs. Nevertheless, issues such as heightened adverse reactions and suboptimal efficacy have arisen. Concerns regarding the quality of low-cost medications and the absence of long-term research have been widely recognized. This has led to caution among patients with late-life depression (LLD) due to their delicate health and the severity of their condition. This study evaluated the choice intention for NVBP drugs and associated factors in older patients with LLD.MethodsA weighted sample of 408 participants between December 2022 and March 2023 were included. Data were collected via face-to-face interviews and questionnaires. To identify significant associated factors of choice intention, a multilevel logistic regression model was employed.ResultsOver half (53.68%) of older patients with LLD intended to choose NVBP drugs. Associated factors included self-assessed poor economy, higher out-of-pocket expenses, monthly household income exceeding CNY 6000, absence of other non-communicable chronic diseases, ordinary registration, urban employee medical insurance, no requirements for brand-name drugs, adverse reactions after using NVBP drugs, and rejection of physicians’ recommendation for NVBP drugs. The interaction effect between the real economic condition and patients self-assessed economy significantly influences choice intention for NVBP drugs. Among 124 patients with self-assessed poor economy, 75 showed a higher intention to use NVBP drugs. In these patients, age, medical insurance reimbursement, and brand awareness were significantly associated with choice intention.ConclusionEconomic factors, physical conditions, medical needs, and physician recommendations significantly influenced the choice intention for NVBP drugs. The choice intention can be improved by strengthening physician-patient communication, increasing the scope and proportion of medical insurance reimbursement, improving substitution studies, and conducting post-marketing re-evaluations of NVBP drugs.

The challenges for psychiatric care posed by synthetic drugs

In addition to the drugs that have been known for decades, several hundred mainly synthetic substances have been identified as drugs for the first time in the last 20years. Presentation of the various groups of substances and their psychotropic effects, the epidemiology of their use and the legal and social background of this development. Narrative literature review. The most important new psychoactive substances (NPS) are synthetic cannabinoids, synthetic stimulants (cathinones), halluginogens and new synthetic opioids (NSO), in particular fentanyl and related substances. The new substances do not have any qualitatively new psychotropic effects. They were brought onto the market in particular as substitutes for substances subject to the Narcotics Act but are often associated with dangerous side effects and even mortality. The increasing availability of these substances has gone hand in hand with the establishment of the Internet as asource of knowledge (e.g. for synthesis routes) and as amarketplace. Substance group-related regulations have also been established in Germany (New Psychoactive Substances Act). In Germany the prevalence of NPS use is significantly lower than that of cannabis; however, there are indications that the production and distribution of synthetic drugs is more profitable for drug dealers than with conventional plant-based drugs, such as heroin. In the USA, for example, NSOs are the primarily drugs used for opioid addiction. It remains to be seen whether NPS and NSOs will replace conventional drugs. The availability of synthetic drugs is more difficult to reduce than that of plant-based drugs. Harm reduction measures should be expanded, e.g., early warning systems for new drugs, drug checking and naloxone programs.

Barriers to access of precision guided therapies for children with high-risk cancer.

Accessing compassionate access schemes to obtain novel therapeutic agents for children with hard-to-treat cancers can be fraught with challenges such as regulatory barriers and limited resources. This study aimed to explore clinician perspectives on the barriers, impacts and ethical considerations of accessing novel therapeutic agents within the context of a paediatric oncology precision medicine trial. We gathered data from 37 semi-structured interviews with paediatric oncologists participating in the PRecISion Medicine for Children with Cancer (PRISM) study, a precision medicine clinical trial in Australia. The interviews, conducted over 2years, focused on paediatric oncologist's experiences with the PRISM trial. Interviews were re-analysed to identify themes related to access pathways and any challenges in obtaining novel agents through thematic analysis. The resulting thematic framework was discussed and refined by a multidisciplinary team. Three main themes were identified: (i) barriers to access, including poor drug availability, lack of evidence and the time burden of the application process; (ii) impacts of inaccessibility, encompassing medical consequences and financial burden on families; and (iii) ethical considerations, centred around balancing realistic expectations and providing compassionate care to patients and families. Paediatric oncologists expressed frustration with the complex regulatory landscape and the lack of systematic reporting on applications and outcomes of obtaining novel agents. Lengthy wait times for decision notifications were also highlighted, raising concerns about missed therapeutic opportunities for patients. This study provides insight to the challenges faced when seeking access to novel therapies for paediatric oncology patients. There is a clear need for improved communication, streamlining processes and increased resources to facilitate access to novel agents. Further resource development is necessary to address these complexities in accessing novel therapy agents to ultimately ensure equitable and timely access.

Exploring the burden of care: challenges faced by caregivers of HIV-infected children in Limpopo Province, South Africa

The number of new HIV cases worldwide has gradually declined; however, South African adolescent females and young women continue to account for the most infections. Although the availability and accessibility of antiretroviral drugs have improved the survival rates of women, some still transmit HIV to their children despite the availability of HIV prevention interventions, resulting in a dual responsibility for the care of themselves and their infected children. This study aims to explore the challenges experienced by caregivers of HIV-infected children in Limpopo Province. The study employed qualitative, exploratory, and descriptive designs, and data was collected from 12 caregivers through semi-structured interviews. Data were analysed using Tesch’s eight steps of analysis. The study identified three challenges facing caregivers of HIV-positive children. Caregivers struggled with disclosure and stigma, finding it difficult to reveal the child's HIV status. The burden of care was significant, including the management of the child's unstable health, ensuring compliance with treatment, and attending frequent hospital visits. The unemployment rate posed a financial burden, particularly in arranging hospital travel costs and providing adequate nutrition to HIV-infected children. These challenges highlight the need for increased support for caregivers of HIV-infected children, including education and awareness campaigns to reduce stigma. Policymakers should consider providing financial support to caregivers. A holistic, multisectoral approach involving healthcare, education, social services, and policymakers is essential.

Nitrate assimilation compensates for cell wall biosynthesis in the absence of Aspergillus fumigatus phosphoglucose isomerase.

Phosphoglucose isomerase (PGI) links glycolysis, the pentose phosphate pathway (PPP), and the synthesis of cell wall precursors in fungi by facilitating the reversible conversion between glucose-6-phosphate (Glc6p) and fructose-6-phosphate (Fru6P). In a previous study, we established the essential role of PGI in cell wall biosynthesis in the opportunistic human fungal pathogen Aspergillus fumigatus, highlighting its potential as a therapeutic target. In this study, we conducted transcriptomic analysis and discovered that the Δpgi mutant exhibited enhanced glycolysis, reduced PPP, and an upregulation of cell wall precursor biosynthesis pathways. Phenotypic analysis revealed defective protein N-glycosylation in the mutant, notably the absence of glycosylated virulence factors DPP V and catalase 1. Interestingly, the cell wall defects in the mutant were not accompanied by activation of the MpkA-dependent cell wall integrity (CWI) signaling pathway. Instead, nitrate assimilation was activated in the Δpgi mutant, stimulating glutamine synthesis and providing amino donors for chitin precursor biosynthesis. Blocking the nitrate assimilation pathway severely impaired the growth of the Δpgi mutant, highlighting the crucial role of nitrate assimilation in rescuing cell wall defects. This study unveils the connection between nitrogen assimilation and cell wall compensation in A. fumigatus.IMPORTANCEAspergillus fumigatus is a common and serious human fungal pathogen that causes a variety of diseases. Given the limited availability of antifungal drugs and increasing drug resistance, it is imperative to understand the fungus' survival mechanisms for effective control of fungal infections. Our previous study highlighted the essential role of A. fumigatus PGI in maintaining cell wall integrity, phosphate sugar homeostasis, and virulence. The present study further illuminates the involvement of PGI in protein N-glycosylation. Furthermore, this research reveals that the nitrogen assimilation pathway, rather than the canonical MpkA-dependent CWI pathway, compensates for cell wall deficiencies in the mutant. These findings offer valuable insights into a novel adaptation mechanism of A. fumigatus to address cell wall defects, which could hold promise for the treatment of infections.

In Vitro Investigation of the Anti-Fibrotic Effects of 1-Phenyl-2-Pentanol, Identified from Moringa oleifera Lam., on Hepatic Stellate Cells.

Liver fibrosis, characterized by excessive extracellular matrix deposition, is driven by activated hepatic stellate cells (HSCs). Due to the limited availability of anti-fibrotic drugs, the research on therapeutic agents continues. Here we have investigated Moringa oleifera Lam. (MO), known for its various bioactive properties, for anti-fibrotic effects. This study has focused on 1-phenyl-2-pentanol (1-PHE), a compound derived from MO leaves, and its effects on LX-2 human hepatic stellate cell activation. TGF-β1-stimulated LX-2 cells were treated with MO extract or 1-PHE, and the changes in liver fibrosis markers were assessed at both gene and protein levels. Proteomic analysis and molecular docking were employed to identify potential protein targets and signaling pathways affected by 1-PHE. Treatment with 1-PHE downregulated fibrosis markers, including collagen type I alpha 1 chain (COL1A1), collagen type IV alpha 1 chain (COL4A1), mothers against decapentaplegic homologs 2 and 3 (SMAD2/3), and matrix metalloproteinase-2 (MMP2), and reduced the secretion of matrix metalloproteinase-9 (MMP-9). Proteomic analysis data showed that 1-PHE modulates the Wnt/β-catenin pathway, providing a possible mechanism for its effects. Our results suggest that 1-PHE inhibits the TGF-β1 and Wnt/β-catenin signaling pathways and HSC activation, indicating its potential as an anti-liver-fibrosis agent.