Plant Drought Research Articles

Growth adaptation of Nitraria sibirica in drought: Hormone-mediated flavonoid accumulation, altered metabolic pathways, and decreased network complexity

Drought is a severe abiotic stress condition that impedes plant growth and development. During adaptation to drought conditions, plants release numerous small-molecule compounds that enhance their adaptive capacity. Nitraria sibirica Pall. is an ecologically valuable plant; however, its metabolic adaptation mechanisms in different soil–water habitats have not been clearly reported. In this study, high-performance liquid chromatography (HPLC) and non-targeted metabolomic techniques were combined to investigate the growth adaptation mechanisms of N. sibirica in different soil environments. The results showed that the response of N. sibirica leaves to drought mainly manifested as a significant increase in abscisic acid (ABA) levels and a significant decrease in gibberellin (GA) levels (except during flowering). Drought led to the production of more metabolites during the growth and development of N. sibirica but reduced the complexity of the metabolite network. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed significant differences in the growth adaptation pathways of metabolites between the two habitats. In riparian habitats (RH), the phenylpropane biosynthesis pathway was significantly upregulated, while the synthesis of cutin, suberin, wax, α-linolenic acid metabolism, and linoleic acid metabolism was significantly downregulated. However, the tryptophan metabolism pathway was consistently downregulated in desert habitats (DH), with flavonoid and flavonol biosynthesis pathways playing important roles in drought resistance. The random forest model showed that the soil environment explained 3.21–94.34 % of the metabolite variation. Furthermore, structural equation modeling demonstrated that soil water had an indirect and positive effect on flavonoid metabolites by regulating ABA. This study revealed the adaptive mechanisms of hormones and metabolites in N. sibirica under drought conditions. It identified certain hormones that could potentially be harnessed for the development of plant additives, providing potential opportunities for exploring new stress adaptation strategies and developing new plant drought resistance products.

Advanced Biotechnological Interventions in Mitigating Drought Stress in Plants.

This comprehensive article critically analyzes the advanced biotechnological strategies to mitigate plant drought stress. It encompasses an in-depth exploration of the latest developments in plant genomics, proteomics, and metabolomics, shedding light on the complex molecular mechanisms that plants employ to combat drought stress. The study also emphasizes the significant advancements in genetic engineering techniques, particularly CRISPR-Cas9 genome editing, which have revolutionized the creation of drought-resistant crop varieties. Furthermore, the article explores microbial biotechnology's pivotal role, such as plant growth-promoting rhizobacteria (PGPR) and mycorrhizae, in enhancing plant resilience against drought conditions. The integration of these cutting-edge biotechnological interventions with traditional breeding methods is presented as a holistic approach for fortifying crops against drought stress. This integration addresses immediate agricultural needs and contributes significantly to sustainable agriculture, ensuring food security in the face of escalating climate change challenges.

Phosphorylation of sugar transporter TST2 by protein kinase CPK27 enhances drought tolerance in tomato.

Drought is a major environmental stress threatening plant growth and productivity. Calcium-dependent protein kinases (CPKs) are plant-specific Ca2+ sensors with multifaceted roles in signaling drought responses. Nonetheless, the mechanisms underpinning how CPKs transmit downstream drought signaling remain unresolved. Through genetic investigations, our study unveiled that knocking out CPK27 reduced drought tolerance in tomato (Solanum lycopersicum) plants and impaired abscisic acid (ABA)-orchestrated plant response to drought stress. Proteomics and phosphoproteomics revealed that CPK27-dependent drought-induced proteins were highly associated with the sugar metabolism pathway, which was further verified by reduced soluble sugar content in the cpk27 mutant under drought conditions. Using protein-protein interaction assays and phosphorylation assessments, we demonstrated that CPK27 directly interacted with and phosphorylated tonoplast sugar transporter 2 (TST2), promoting intercellular soluble sugar accumulation during drought stress. Furthermore, Ca2+ and ABA enhanced CPK27-mediated interaction and phosphorylation of TST2, thus revealing a role of TST2 in tomato plant drought tolerance. These findings extend the toolbox of potential interventions for enhancing plant drought stress tolerance and provide a target to improve drought tolerance by manipulating CPK27-mediated soluble sugar accumulation for rendering drought tolerance in a changing climate.

Plant species within Streptanthoid Complex associate with distinct microbial communities that shift to be more similar under drought.

Prolonged water stress can shift rhizoplane microbial communities, yet whether plant phylogenetic relatedness or drought tolerance predicts microbial responses is poorly understood. To explore this question, eight members of the Streptanthus clade with varying affinity to serpentine soil were subjected to three watering regimes. Rhizoplane bacterial communities were characterized using 16S rRNA gene amplicon sequencing and we compared the impact of watering treatment, soil affinity, and plant species identity on bacterial alpha and diversity. We determined which taxa were enriched among drought treatments using DESeq2 and identified features of soil affinity using random forest analysis. We show that water stress has a greater impact on microbial community structure than soil affinity or plant identity, even within a genus. Drought reduced alpha diversity overall, but plant species did not strongly differentiate alpha diversity. Watering altered the relative abundance of bacterial genera within Proteobacteria, Firmicutes, Bacteroidetes, Planctomycetes, and Acidobacteria, which responded similarly in the rhizoplane of most plant species. In addition, bacterial communities were more similar when plants received less water. Pseudarthrobacter was identified as a feature of affinity to serpentine soil while Bradyrhizobium, Chitinophaga, Rhodanobacter, and Paenibacillus were features associated with affinity to nonserpentine soils among Streptanthus. The homogenizing effect of drought on microbial communities and the increasing prevalence of Gram-negative bacteria across all plant species suggest that effects of water stress on root-associated microbiome structure may be predictable among closely related plant species that inhabit very different soil environments. The functional implications of observed changes in microbiome composition remain to be studied.

Effect of plant density and drought stress on seed productivity and biochemical composition of faba bean plants

Effect of plant density and drought stress on seed productivity and biochemical composition of faba bean plants

Evolutionary and expression analysis of sugar transporters from Tartary buckwheat revealed the potential function of FtERD23 in drought stress

Drought is becoming a common threat to crop production. To combat this stress and ensure global food security, the identification and utilization of excellent drought-resistant genes are crucial for developing drought-resistant crop varieties. However, sugar transporters are known to be involved in stress tolerance in many plants, while the sugar transporter gene family of Tartary buckwheat has not been systematically analyzed yet. In this study, 140 sugar transporter genes were identified from the ‘Pinku’ Tartary buckwheat genome and classified into ten subfamilies. Structural analysis showed that subfamily SGB/pGlcT had the highest number of introns compared to other subfamilies, and abundant abiotic stress-related cis-acting elements existed in the promoter region. Collinear analysis revealed that FtSUT7, FtSTP28, FtPLT1, and FtINT2 genes are relatively ancient. The expression of sugar transporter genes was screened under various abiotic stresses which revealed the association of stress tolerance with different sugar transporter genes, i.e., FtERD23, FtINT2, FtpGlcT2, and FtSTP27. Further, it was observed that the overexpression of FtERD23 maintains osmotic pressure through glucose transport, which may enhance drought stress tolerance. Moreover, gene co-expression analyses using WGCNA and FCMA identified six transcription factors that may regulate FtERD23 expression and are involved in plant drought tolerance. In summary, this systematic analysis provides a theoretical basis for further functional characterization of sugar transporter genes to improve drought tolerance in Tartary buckwheat and its related species.

Regulation of endogenous hormone and miRNA in leaves of alfalfa (Medicago sativa L.) seedlings under drought stress by endogenous nitric oxide

BackgroundAlfalfa (Medicago sativa. L) is one of the best leguminous herbage in China and even in the world, with high nutritional and ecological value. However, one of the drawbacks of alfalfa is its sensitivity to dry conditions, which is a global agricultural problem. The objective of this study was to investigate the regulatory effects of endogenous nitric oxide (NO) on endogenous hormones and related miRNAs in alfalfa seedling leaves under drought stress. The effects of endogenous NO on endogenous hormones such as ABA, GA3, SA, and IAA in alfalfa leaves under drought stress were studied. In addition, high-throughput sequencing technology was used to identify drought-related miRNAs and endogenous NO-responsive miRNAs in alfalfa seedling leaves under drought stress.ResultBy measuring the contents of four endogenous hormones in alfalfa leaves, it was found that endogenous NO could regulate plant growth and stress resistance by inducing the metabolism levels of IAA, ABA, GA3, and SA in alfalfa, especially ABA and SA in alfalfa. In addition, small RNA sequencing technology and bioinformatics methods were used to analyze endogenous NO-responsive miRNAs under drought stress. It was found that most miRNAs were enriched in biological pathways and molecular functions related to hormones (ABA, ETH, and JA), phenylpropane metabolism, and plant stress tolerance.ConclusionIn this study, the analysis of endogenous hormone signals and miRNAs in alfalfa leaves under PEG and PEG + cPTIO conditions provided an important basis for endogenous NO to improve the drought resistance of alfalfa at the physiological and molecular levels. It has important scientific value and practical significance for endogenous NO to improve plant drought resistance.

Rhizosheath drought responsiveness is variety-specific and a key component of belowground plant adaptation.

Biophysicochemical rhizosheath properties play a vital role in plant drought adaptation. However, their integration into the framework of plant drought response is hampered by incomplete mechanistic understanding of their drought responsiveness and unknown linkage to intraspecific plant-soil drought reactions. Thirty-eight Zea mays varieties were grown under well-watered and drought conditions to assess the drought responsiveness of rhizosheath properties, such as soil aggregation, rhizosheath mass, net-rhizodeposition, and soil organic carbon distribution. Additionally, explanatory traits, including functional plant trait adaptations and changes in soil enzyme activities, were measured. Drought restricted soil structure formation in the rhizosheath and shifted plant-carbonfrom litter-derived organic matter in macroaggregates to microbially processed compounds in microaggregates. Variety-specific functional trait modifications determined variations in rhizosheath drought responsiveness. Drought responses of the plant-soil system ranged among varieties from maintaining plant-microbial interactions in the rhizosheath through accumulation of rhizodeposits, to preserving rhizosheath soil structure while increasing soil exploration through enhanced root elongation. Drought-induced alterations at the root-soil interface may hold crucial implications for ecosystem resilience in a changing climate. Our findings highlight that rhizosheath soil properties are an intrinsic component of plant drought response, emphasizing the need for a holistic concept of plant-soil systems in future research on plant drought adaptation.

Tomato plant growth promotion and drought tolerance conferred by three arbuscular mycorrhizal fungi is mediated by lipid metabolism

Arbuscular mycorrhizal fungi (AMF) can promote plant growth and enhance plant drought tolerance with varying effect size among different fungal species. However, the linkage between the variation and the lipid metabolism, which is exclusively derived from plants, has been little explored thus far. Here, we established AM symbiosis between tomato (Solanum lycopersicum) plants and three AMF species (Rhizophagus intraradices, Funneliformis mosseae, Rhizophagus irregularis) under well watered (WW) or drought stressed (DS) conditions in pot experiment. The plant biomass, chlorophyll fluorescence Fv/Fm, shoot P content and mycorrhizal colonization were determined. Meanwhile, fatty acid (FA) profiles and relative expression of genes encoding for nutrition exchange (SlPT4, SlPT5, RAM2, STR/STR2) in roots were also monitored. DS significantly decreased plant biomass while AMF significantly increased it, with three fungal species varying in their growth promoting capacity and drought tolerance capacity. The growth promoting effect of R. irregularis was lower than those of R. intraradices and F. mosseae, and was associated with higher mycorrhizal colonization and more consumption of lipids. However, the drought tolerance capacity of R. irregularis was greater than those of R. intraradices and F. mosseae, and was associated with less decrease in mycorrhizal colonization and lipid content. We also found that AMF mediated plant drought tolerance via regulating both AM specific FAs and non-AM specific FAs in a complementary manner. These data suggest that lipid metabolism in AM plays a crucial role in plant drought tolerance mediated by AMF.

Bioprospecting the roles of Trichoderma in alleviating plants’ drought tolerance: Principles, mechanisms of action, and prospects

Drought-induced stress represents a significant challenge to agricultural production, exerting adverse effects on both plant growth and overall productivity. Therefore, the exploration of innovative long-term approaches for addressing drought stress within agriculture constitutes a crucial objective, given its vital role in enhancing food security. This article explores the potential use of Trichoderma, a well-known genus of plant growth-promoting fungi, to enhance plant tolerance to drought stress. Trichoderma species have shown remarkable potential for enhancing plant growth, inducing systemic resistance, and ameliorating the adverse impacts of drought stress on plants through the modulation of morphological, physiological, biochemical, and molecular characteristics. In conclusion, the exploitation of Trichoderma's potential as a sustainable solution to enhance plant drought tolerance is a promising avenue for addressing the challenges posed by the changing climate. The manifold advantages of Trichoderma in promoting plant growth and alleviating the effects of drought stress underscore their pivotal role in fostering sustainable agricultural practices and enhancing food security.

Drought Stress Induced Different Response Mechanisms in Three Dendrobium Species under Different Photosynthetic Pathways.

Many Dendrobium species, which hold a high status and value in traditional Chinese medicine, grow on barks and rocks in the wild, often encountering harsh environments and facing droughts. However, the molecular mechanisms underlying the shift in the photosynthetic pathway induced by drought remain unclear. To address this issue, three Dendrobium species with different photosynthetic pathways were selected for sequencing and transcriptome data analysis after drought treatment. The findings included 134.43 GB of sequencing data, with numerous Differentially Expressed Genes (DEGs) exhibiting different response mechanisms under drought stress. Gene Ontology (GO)-KEGG-based enrichment analysis of DEGs revealed that metabolic pathways contributed to drought tolerance and alterations in photosynthetic pathways. Phosphoenolpyruvate Carboxylase (PEPC) was subjected to phylogenetic tree construction, sequence alignment, and domain analysis. Under drought stress, variations were observed in the PEPC gene structure and expression among different Dendrobium species; the upregulation of Dc_gene2609 expression may be caused by dof-miR-384, which resulted in the shift from C3 photosynthesis to CAM, thereby improving drought tolerance in Dendrobium. This study revealed the expression patterns and roles of PEPC genes in enhancing plant drought tolerance and will provide an important basis for in-depth research on Dendrobium's adaptation mechanisms in arid environments.

AI-assisted image analysis and physiological validation for progressive drought detection in a diverse panel of Gossypium hirsutum L.

Drought detection, spanning from early stress to severe conditions, plays a crucial role in maintaining productivity, facilitating recovery, and preventing plant mortality. While handheld thermal cameras have been widely employed to track changes in leaf water content and stomatal conductance, research on thermal image classification remains limited due mainly to low resolution and blurry images produced by handheld cameras. In this study, we introduce a computer vision pipeline to enhance the significance of leaf-level thermal images across 27 distinct cotton genotypes cultivated in a greenhouse under progressive drought conditions. Our approach involved employing a customized software pipeline to process raw thermal images, generating leaf masks, and extracting a range of statistically relevant thermal features (e.g., min and max temperature, median value, quartiles, etc.). These features were then utilized to develop machine learning algorithms capable of assessing leaf hydration status and distinguishing between well-watered (WW) and dry-down (DD) conditions. Two different classifiers were trained to predict the plant treatment-random forest and multilayer perceptron neural networks-finding 75% and 78% accuracy in the treatment prediction, respectively. Furthermore, we evaluated the predicted versus true labels based on classic physiological indicators of drought in plants, including volumetric soil water content, leaf water potential, and chlorophyll a fluorescence, to provide more insights and possible explanations about the classification outputs. Interestingly, mislabeled leaves mostly exhibited notable responses in fluorescence, water uptake from the soil, and/or leaf hydration status. Our findings emphasize the potential of AI-assisted thermal image analysis in enhancing the informative value of common heterogeneous datasets for drought detection. This application suggests widening the experimental settings to be used with deep learning models, designing future investigations into the genotypic variation in plant drought response and potential optimization of water management in agricultural settings.

Plant tolerance to drought stress: Complexity and mechanism across physiological, molecular and biochemical scales

Drought stress limits crop yield globally and is growing as a result of climate change. Water deficit of soil frequently reduces crop growth and yield regardless of developmental stages and nature of genotypes. There are a variety of mechanisms involved in plants in response to drought stress and they trigger the plant drought tolerance strategies. Plants subjected to drought undergo a sequence of physiological, biochemical, and molecular mechanisms to combat the effects of drought, just like plants exposed to other environmental stressors. Using various methods, researchers are currently attempting to understand the intricate operation of the drought stress response in plants. During particular crucial stages such as seed germination, and formation of seedlings, flowering, and grains, plants are particularly vulnerable to drought stress. Through the activation of tolerance mechanisms, plants successfully mitigate the impacts of drought stress during vegetative development stages. However, drought stress during the generative phase might result in yield losses that are irreversible. The present review highlights the tolerance mechanisms in plants and the functioning of physiological, molecular, and biochemical processes involved in plants' resistance to water deficit as well as how various crops react to drying conditions at various developmental points.

Screening cotton genotypes for their drought tolerance ability based on the expression level of dehydration-responsive element-binding protein and proline biosynthesis-related genes and morpho-physio-biochemical responses.

Drought stress adversely affects growth, development, productivity, and fiber quality of cotton (Gossypium hirsutum L). Breeding strategies to enhance drought tolerance require an improved knowledge of plant drought responses necessitating proper identification of drought-tolerant genotypes of crops, including cotton. The objective of this study was to classify the selected cotton genotypes for their drought tolerance ability based on morpho-physio-biochemical traits using Hierarchical Ward's cluster analysis. Five genotypes of cotton (Takfa 3, Takfa 6, Takfa 7, Takfa 84-4, and Takfa 86-5) were selected as plant materials, and were grown under well-watered (WW; 98 ± 2% field capacity) and water-deficit (WD; 50 ± 2% field capacity) conditions for 16days during the flower initiation stage. Data on morpho-physio-biochemical parameters and gene expression levels for these parameters were collected, and subsequently genotypes were classified either as a drought tolerant or drought susceptible one. Upregulation of GhPRP (proline-rich protein), GhP5CS (Δ1-pyrroline-5-carboxylate synthetase), and GhP5CR (Δ1-pyrroline-5-carboxylate reductase) in relation to free proline enrichment was observed in Takfa 3 genotype under WD condition. An accumulation of free proline, total soluble sugar, and potassium in plants under WD conditions was detected, which played a key role as major osmolytes controlling cellular osmotic potential. Magnesium and calcium concentrations were also enriched in leaves under WD conditions, functioning as essential elements and regulating photosynthetic abilities. Leaf greenness, net photosynthetic rate, stomatal conductance, and transpiration rate were also declined under WD conditions, leading to growth retardation, especially aboveground traits of Takfa 6, Takfa 7, Takfa 84-4, and Takfa 86-5 genotypes. An increase in leaf temperature (1.1 - 4.0°C) and crop water stress index (CWSI > 0.75) in relation to stomatal closure and reduced transpiration rate was recorded in cotton genotypes under WD conditions compared with WW conditions. Based on the increase of free proline, soluble sugar, leaf temperature, and CWSI, as well as the decrease of aboveground growth traits and physiological attributes, five genotypes were categorized into two cluster groups: drought tolerant (Takfa 3) and drought susceptible (Takfa 6, Takfa 7, Takfa 84-4, and Takfa 86-5). The identified drought-tolerant cotton genotype, namely, Takfa 3, may be grown in areas experiencing drought conditions. It is recommended to further validate the yield traits of Takfa 3 under rainfed field conditions in drought-prone environments.

GhWRKY4 binds to the histone deacetylase GhHDA8 promoter to regulate drought and salt tolerance in Gossypium hirsutum

Soil drought and salinization, caused by water deficiency, have become the greatest concerns limiting crop production. Up to now, the WRKY transcription factor and histone deacetylase have been shown to be involved in drought and salt responses. However, the molecular mechanism underlying their interaction remains unclear in cotton. Herein, we identified GhWRKY4, a member of WRKY gene family, which is induced by drought and salt stress and is located in the nucleus. The ectopic expression of GhWRKY4 in Arabidopsis enhanced drought and salt tolerance, and suppressing GhWRKY4 in cotton increased susceptibility to drought and salinity. Subsequently, DAP-seq analysis revealed that the W box element in the promoter of stress-induced genes could potentially be the binding target for GhWRKY4 protein. GhWRKY4 binds to the promoters of GhHDA8 and GhNHX7 via W box element, and the expression level of GhHDA8 was increased in GhWRKY4-silenced plants. In addition, GhHDA8-overexpressed Arabidopsis were found to be hypersensitive to drought and salt stress, while silencing of GhHDA8 enhanced drought and salt tolerance in cotton. The stress-related genes, such as GhDREB2A, GhRD22, GhP5CS, and GhNHX7, were induced in GhHDA8-silenced plants. Our findings indicate that the GhWRKY4-GhHDA8 module regulates drought and salt tolerance in cotton. Collectively, the results provide new insights into the coordination of transcription factors and histone deacetylases in regulating drought and salt stress responses in plants.

The Emerging Role of Non-Coding RNAs (ncRNAs) in Plant Growth, Development, and Stress Response Signaling.

Plant species utilize a variety of regulatory mechanisms to ensure sustainable productivity. Within this intricate framework, numerous non-coding RNAs (ncRNAs) play a crucial regulatory role in plant biology, surpassing the essential functions of RNA molecules as messengers, ribosomal, and transfer RNAs. ncRNAs represent an emerging class of regulators, operating directly in the form of small interfering RNAs (siRNAs), microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs). These ncRNAs exert control at various levels, including transcription, post-transcription, translation, and epigenetic. Furthermore, they interact with each other, contributing to a variety of biological processes and mechanisms associated with stress resilience. This review primarily concentrates on the recent advancements in plant ncRNAs, delineating their functions in growth and development across various organs such as root, leaf, seed/endosperm, and seed nutrient development. Additionally, this review broadens its scope by examining the role of ncRNAs in response to environmental stresses such as drought, salt, flood, heat, and cold in plants. This compilation offers updated information and insights to guide the characterization of the potential functions of ncRNAs in plant growth, development, and stress resilience in future research.

Drought Stress Inhibits Starch Accumulation in Taro (Colocasia esculenta L. Schott).

Colocasia esculenta L. Schott is a main traditional root crop in China, serving as an important vegetable and staple food. Drought stress plays vital role on the growth and development of taro corm. Two different varieties of taro in Jiangsu were selected: Xiangsha taro and Longxiang taro. The accumulation characteristics, morphological structure, and physicochemical properties of taro corm starch were studied by microscopic observation, particle size analysis, and X-ray diffractometer (XRD) analysis. Transcriptome analyses were used to identify the related genes of taro corm under drought stress. During the growth of taro, the number of amyloplasts showed an obvious increasing trend and shifted from being dispersed throughout the cells to being gathered on one side of the cells, and morphological observations showed that smaller granular distribution gradually changed to a larger lumpy distribution. The particle size of Longxiang taro is smaller than that of Xiangsha taro. Under drought stress conditions, the occurrence of starch grains and corm size were inhibited in Xiangsha taro. Transcriptome sequencing of drought-stressed taro corms showed that the enzymes related to starch synthesis were differentially expressed. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of drought-stressed taro corms showed that drought affected hormone signal transduction, material metabolism, drought stress tolerance, plant growth and development, and stress resistance, which triggered the plant drought adaptive response. Drought stress inhibits starch accumulation in taro.

PwuWRKY48 Confers Drought Tolerance in Populus wulianensis

Populus wulianensis mainly grows in hilly and sloped areas and has strong resistance to adversity. Previous transcriptome studies have shown that a WRKY gene PwuWRKY48 is expression-induced under drought stress. In this study, we aim to characterize the gene’s structure and investigate its role in plant drought resistance. The results show that PwuWRKY48 (1113 bp) belongs to a class IIc WRKY subfamily and it was determined as a nuclear localization protein. The gene promoter region contains a variety of cis-elements in relation to stress resistance. Under drought stress, PwuWRKY48 was expression-induced in leaves and stems, 29.7 and 16.6 times those before treatment, respectively. Overexpressing PwuWRKY48 lines were associated with increased activities of peroxidase (POD) and superoxide dismutase (SOD), 2.5 and 1.6 times higher than those of the wild type. While malondialdehyde content (MDA), superoxide anion radical (O2·−), and relative conductivity were decreased by 20%, 30%, and 21.3%, proline and chlorophyll contents increased by 37.5% and 11.2%, respectively. This indicates that PwuWRKY48 efficiently improved the drought tolerance of transgenic plants. PwuWRKY48 can be used as a gene resource for molecular breeding of plant drought resistance.

Transcriptomic Insights into Abies koreana Drought Tolerance Conferred by Aureobasidium pullulans AK10.

The conservation of the endangered Korean fir, Abies koreana, is of critical ecological importance. In our previous study, a yeast-like fungus identified as Aureobasidium pullulans AK10, was isolated and shown to enhance drought tolerance in A. koreana seedlings. In this study, the effectiveness of Au. pullulans AK10 treatment in enhancing drought tolerance in A. koreana was confirmed. Furthermore, using transcriptome analysis, we compared A. koreana seedlings treated with Au. pullulans AK10 to untreated controls under drought conditions to elucidate the molecular responses involved in increased drought tolerance. Our findings revealed a predominance of downregulated genes in the treated seedlings, suggesting a strategic reallocation of resources to enhance stress defense. Further exploration of enriched Kyoto Encyclopedia of Genes and Genomes pathways and protein-protein interaction networks revealed significant alterations in functional systems known to fortify drought tolerance, including the terpenoid backbone biosynthesis, calcium signaling pathway, pyruvate metabolism, brassinosteroid biosynthesis, and, crucially, flavonoid biosynthesis, renowned for enhancing plant drought resistance. These findings deepen our comprehension of how AK10 biostimulation enhances the resilience of A. koreana to drought stress, marking a substantial advancement in the effort to conserve this endangered tree species through environmentally sustainable treatment.

Genome-wide genetic architecture for plant maturity and drought tolerance in diploid potatoes.

Cultivated potato (Solanum tuberosum) is known to be highly susceptible to drought. With climate change and its frequent episodes of drought, potato growers will face increased challenges to achieving their yield goals. Currently, a high proportion of untapped potato germplasm remains within the diploid potato relatives, and the genetic architecture of the drought tolerance and maturity traits of diploid potatoes is still unknown. As such, a panel of 384 ethyl methanesulfonate-mutagenized diploid potato clones were evaluated for drought tolerance and plant maturity under field conditions. Genome-wide association studies (GWAS) were conducted to dissect the genetic architecture of the traits. The results obtained from the genetic structure analysis of the panel showed five main groups and seven subgroups. Using the Genome Association and Prediction Integrated Tool-mixed linear model GWAS statistical model, 34 and 17 significant quantitative trait nucleotides (QTNs) were found associated with maturity and drought traits, respectively. Chromosome 5 carried most of the QTNs, some of which were also detected by using the restricted two-stage multi-locus multi-allele-GWAS haploblock-based model, and two QTNs were found to be pleiotropic for both maturity and drought traits. Using the non-parametric U-test, one and three QTNs, with 5.13%-7.4% phenotypic variations explained, showed favorable allelic effects that increase the maturity and drought trait values. The quantitaive trait loci (QTLs)/QTNs associated with maturity and drought trait were found co-located in narrow (0.5-1kb) genomic regions with 56 candidate genes playing roles in plant development and senescence and in abiotic stress responses. A total of 127 potato clones were found to be late maturing and tolerant to drought, while nine were early to moderate-late maturing and tolerant to drought. Taken together, the data show that the studied germplasm panel and the identified candidate genes are prime genetic resources for breeders and biologists in conventional breeding and targeted gene editing as climate adaptation tools.