Structure Of Droplets Research Articles

High internal phase Pickering emulsions stabilized by tannic acid-ovalbumin complexes: Interfacial property and stability

While protein-stabilized high internal phase Pickering emulsions (HIPEs) have demonstrated promising potential for various applications in food, their poor interfacial stability remains as a major challenge to overcome. In this work, tannic acid (TA) was used to increase the stability of ovalbumin (OVA)-stabilized HIPEs. The binding clusters between OVA and TA were hypothesized by the molecular docking simulation, and the interfacial properties of OVA and TA complexes (OVA-TA) were investigated by interfacial tension and three-phase contact angle. The characteristics of HIPEs under different pHs were studied by static light scattering and confocal laser scanning microscopy (CLSM), as well as rheology. The results showed that there were some specific clusters in OVA and TA which may form hydrogen bonds and hydrophobic interactions. The wettability of OVA was significantly improved by TA, resulting in greater interfacial stability at the oil-water interface. Compared with OVA-stabilized HIPEs (O-HIPEs), OVA-TA-stabilized HIPEs (OT-HIPEs) had narrower size distribution, more compact droplet structure and denser gel network to resist droplet aggregation. Especially at pH 4.0, OT-HIPEs exhibited stronger gel network and higher viscosity than at other pH values, because of smaller electrostatic repulsion between OVA and TA, and easier crosslinking on the oil-water interface. In addition, the viscosity of OT-HIPEs was increased after 24 h incubation, indicating the formation of more hydrogen bonds. Meanwhile, OT-HIPEs showed good storage and freeze-thaw stability. Our research findings from this study are expected to provide novel insight on protein-based particles stabilized HIPEs from an interfacial perspective.

Rice OsClo5, a caleosin protein, negatively regulates cold tolerance through the jasmonate signalling pathway.

Caleosin is a lipid droplet-binding protein involved in maintenance of the lipid droplet structure and in signal transduction. However, the role of caleosin proteins in stress resistance is limited. Here, we report data for a rice caleosin protein gene, OsClo5, involved in cold stress tolerance via influence and regulation of the JA signalling pathway. Overexpression lines and RNAi lines of OsClo5 were subjected to cold stress and recovery to measure electrolyte leakage and survival rate. Changes were also detected in the genome-wide transcriptome of OsClo5 overexpressed plants. OsClo5 is located mainly in lipid droplets and expressed in all tissues tested. Its expression was upregulated by various stress conditions when subjected to cold treatment. Overexpression of OsClo5 decreased cold tolerance, and RNAi lines of OsClo5 had higher survival than WT seedlings. OsClo5 inhibited one jasmonate biosynthetic gene and several jasmonate ZIM domain (JAZ) genes, which were upregulated in response to cold stress. OsClo5 is a constitutively expressed caleosin protein that regulates plant cold resistance through inhibition of jasmonate signalling and JA synthesis.

Flow Cytometry Optimization of Three Different BODIPY Molecules for Staining Lipid Droplets of Cryptococcus neoformans

The pathogenic Cryptococcus neoformans causes life-threatening disease in immunocompromised patients. Although there are hypotheses about the role of lipid droplets in C.neoformans pathogenesis, there is still not extensively data determined on the subject yet. Lipid droplets are dynamic cytoplasmic energy storage bodies in yeasts. Diazo dyes, Nile red, LD540 and borradiazaindasen (BODIPY) molecules are frequently used in the lipid droplets studies. BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyestuffs are among the brightest green light emitting fluorophores. These neutral molecules have high lipophilicity and can easily pass through the cell wall and membrane. In this study, for the future studies on lipid droplets of C.neoformans, we aimed to optimize three different BODIPY (BODIPY480/525, BODIPY480/530, BODIPY480/535) molecules for fluorescence microscopy and flow cytometry system. Ten molecularly confirmed environmental C.neoformans strains were grown on Sabouraud dextrose agar with and without oleic acid. BODIPY staining protocols at different concentrations were used for C.neoformans lipid droplets fixed with paraformaldehyde. The visualization (by fluorescence microscopy) and detection (by flow cytometer) of the lipid droplet structures of C.neoformans strains were evaluated. Forwardscatter and side-scatter analysis were performed to evaluate the number of lipid droplets determined in the cytoplasmic region quadrant in flow cytometry. The staining of the lipid droplets of C.neoformans of all three BODIPY molecules used in the study was observed by fluorescence microscope with creating distinct brightness and sharp contrast with the background. All BODIPY molecules could be examined by fluorescence microscopy without loss of brightness in more than one minute. The optimal dye concentration of BODIPY compounds were found as 2 µM. Incubation at room temperature for five minutes was sufficient for fluorochrome staining. They were also shown to be able to stain lipid droplets in heatinactivated C.neoformans strains in all three compounds. The synthesized BODIPY480/525, BODIPY480/530 and BODIPY480/535 molecules were evaluated in accordance with the staining of lipid droplets, which were claimed to play a role in the pathogenesis of C.neoformans, and analysis by fluorescence microscopy and analysis with flow cytometer. BODIPY molecules may exhibit different properties in staining lipid droplets. These molecules should be tested for demonstrating the presence of lipid droplets in different yeast species and their suitability for pathogenesis studies.

Novel dispersant based on the synergy of nickel hydroxide and sulfonated lignin for applications in oil spill remediation

Chemical dispersants are a controversial solution for oil spill remediation, and the potential toxicity of chemical surfactants to the marine ecology has prompted much research for eco-friendly dispersants with superior dispersing ability. Herein, an efficient Pickering emulsifier was prepared on the basis of the synergistic effect of sulfonated lignin (SL) and nickel hydroxide (Ni(OH)2) micron particles. The interaction of SL and Ni(OH)2 particles was characterized by the contact angle, shear viscosity and Zeta potential measurements. Confocal fluorescence microscopy and optical microscopy images revealed the oil droplet structure. The average emulsion droplet size and emulsification index were investigated. These results indicate that the addition of SL has a synergistic stabilizing effect in high salinity seawater. Here, Ni(OH)2 particles alone are not an efficient emulsifier in seawater because of the strong flocculation caused by high salinity. The adsorption of Ni(OH)2 particles by SL changed their contact angle and benefitted their adsorption at the oil-seawater interface, providing a solid barrier to avoid droplet coalescence, and the stable O/W emulsion exhibited great stability, smaller droplet size and larger oil-water interface area, which facilitated the subsequent natural degradation of crude oil. Therefore, this may be an environmentally friendly and harmless new method for SL and Ni(OH)2 particles to deal with marine oil spill problems.

Tunable crystal structures of binary mixtures of various patchy colloids and droplets

In this work, a series of binary mixtures of patchy colloids with desired symmetries, including quadrangle, pentagon, hexagon, is studied by means of Metropolis Monte Carlo simulations. The patches on the colloid surface attract the droplets via the Pickering effect, while both the colloid-colloid and the droplet-droplet interactions are pure hard-core. We find a rich phase diagram by tuning the size ratio, particle packing fraction, angle parameter. The four-patch and six-patch colloids can easily assemble into the different crystal structures, while the five-patch colloids can form a local ordered state that is similar to a quasicrystal with a five-fold symmetry. In the self-assembled structures of six-patch colloids, both droplets and colloids form hexagonal orders, however, they arrange in themselves on different sublattices. Considering the four patches in a quadrilateral geometry, we show that the simulated systems undergoes a structural transition from a rectangle lattice to square lattice and then to rhombohedral lattice upon changing the characteristic angle. In addition, the absence of crystal structures in the trapezoidal patches suggests that the geometrical constraint in order to form the crystal states is that the patchy pattern needs to have the inversion center located at the diameter of the colloidal sphere.

Formation and arsenic distribution of egg-type structure of matte droplets inside the copper smelting slag

Arsenic (As) element is always accompanied with Cu-rich phase and forms egg-type structure in the copper slag concentrate through beneficiation process, which means that As element may be recycled through beneficiation process and therefore increase the burden of smelting system. In this work, the formation mechanism of egg-type structure in the slag is systemically studied through thermodynamics and dynamics, which may provide theoretic guidance to separate As and Cu elements in the copper smelting system.

Lattice Boltzmann and Jones matrix calculations for the determination of the director field structure in self-propelling nematic droplets.

Nematic droplets immersed in aqueous surfactant solutions can show a self-propelled motion induced by a Marangoni flow in the droplet surface. In addition to the self-propulsion, the Marangoni flow induces within the droplet a convective flow which considerably influences the nematic director field of the droplet. We report numerical simulations aiming at the determination of the director field in the self-propelling droplet. The convective flow and the resulting structure of director field are described by a lattice Boltzmann model. The reliability of the obtained structures is proved by subsequent Jones matrix calculations which enable the direct comparison of experimental polarizing microscopy images of self-propelling droplets with calculated images based on the simulated structures.

Modeling the dynamics of charged drop of one liquid in another under the action of an electric field

The work is devoted to the study of the features of the behavior of a group of droplets of one viscous liquid in another under the influence of various physical fields. When considering the dynamics of two drops under the action of an electric field it is assumed that a drop in the form of a sphere with radius а will be placed in an electric field with an intensity E , investigates how droplets will react to each other under the influence of an electric field. A mathematical model has been built and a computer program has been developed for the nu- merical solution of this problem. The behavior of several drops in an electric field is studied for different physical parameters of the material of the drops and the environment, as well as for different initial distribu- tions of drops and the strength of the electric field. It is shown for the first time that emulsion droplets distrib- uted in space, under the action of an electric field, begin to move and after a certain time a new stationary structure of droplets is formed. It was found that the relaxation time depends on the electric field strength, the size of the droplets and their initial distribution.

The impact of pH on mechanical properties, storage stability and digestion of alginate-based and soy protein isolate-stabilized emulsion gel beads with encapsulated lycopene

In alginate-based emulsion gels containing protein-coated droplets, pH can influence the gelation mechanism of alginate gels, and the interactions between alginate molecules and protein-coated droplets, and thus properties of emulsion gels. This study investigated the impact of pH 3–7 on the properties (e.g., surface structures of droplets, mechanical properties, storage stability, digestion behavior) of alginate gel beads containing soy protein isolate(SPI)-stabilized oil droplets. Emulsion droplets were SPI-coated droplets at pH 6–7 and alginate/SPI-coated droplets at pH 3–5. Emulsion droplet flocculation only occurred in emulsions at pH 7.0. Emulsion gel beads at pH 3.0 had lower mechanical strength, higher storage stability, faster release of encapsulated lycopene during in-vitro digestion, and higher bioaccesibility of lycopene after 2 h of intestinal digestion than those at pH 7.0 and 5.0. The findings of this study are crucial to emulsion gel beads with controlled release and improved storage stability of encapsulated compounds by changing the pH of emulsions.

Transient hexagonal structures in sheared emulsions of isotropic inclusions on smectic bubbles in microgravity conditions

We describe the collective behavior of isotropic droplets dispersed over a spherical smectic bubble, observed under microgravity conditions on the International Space Station (ISS). We find that droplets can form two-dimensional hexagonal structures changing with time. Our analysis indicates the possibility of spatial and temporal periodicity of such structures of droplets. Quantitative analysis of the hexagonal structure including the first three coordination circles was performed. A peculiar periodic-in-time ordering of the droplets, related to one-dimensional motion of droplets with non-uniform velocity, was found.

The invisible arbiter of emulsion structure: insight from an examination of two types of 3-phase emulsions

It has long been established that the equilibrium properties of a water–oil–surfactant system governs the morphology of the emulsion formed—an inherently non-equilibrium system. In this work, we reinforce this concept and bring forward additional insights. Two types of 3-phase emulsions are examined, one obtained from suspension polymerization of silicone and acrylate in an aqueous phase and another obtained from mechanical dispersion of a silicone oil and a sunflower seed oil into an aqueous phase. We show that the specific emulsification process has an impact on the detailed structure of the final multiple droplets. In suspension polymerization, the complete miscibility of silicone with acrylate monomers prior to polymerization renders uniformity in the composition of the starting droplets; hence the final droplets after polymerization all have identical equilibrium tensions as well as relative volume ratios between the separated phases. Consequently, the final droplets have uniformly the same structure as predicted based on thermodynamics. On the other hand, the 3-phase emulsion formed from mechanical dispersion of two immiscible oils in water shows an overall morphology that is predicted by equilibrium tensions, however, a wide variation in the specific geometry is observed. This latter aspect reflects the randomness in drop break-up-and-recombination associated with the homogenization process, which results in variable volume ratio between the two immiscible oils among different droplets.

High fat / high cholesterol diet does not provoke atherosclerosis in the ω3-and ω6-polyunsaturated fatty acid synthesis–inactivated Δ6-fatty acid desaturase–deficient mouse

ObjectiveAn increased ω6/ω3-polyunsaturated fatty acid ratio in the current Western diet is regarded as a critical epigenetic nutritional factor in the pathogenesis of several human lifestyle diseases, metabolic syndrome, cardiovascular disease, the central nervous system and the female and male reproductive systems. The impact of nutrient ω3-and ω6-PUFAs in the pathogenesis of dyslipoproteinemia and atherosclerosis has been a topic of intense efforts for several decades. Cellular homeostasis of the ω3-and ω6- PUFA pool is maintained by the synthesis of ω3-and ω6-PUFAs from essential fatty acids (EFA) (linoleic and α-linolenic acid) and their dietary supply.In this study, we used the auxotrophic Δ6-fatty acid desaturase- (FADS2) deficient mouse (fads2−/−), an unbiased model congenial for stringent feeding experiments, to investigate the molecular basis of the proposed protective role of dietary ω3-and ω6-PUFAs (Western diet) in the pathogenesis of multifactorial dyslipoproteinemia and atherosclerosis. We focused on the metabolic axis—liver endoplasmic reticulum (ER), serum lipoprotein system (Lp) and aorta vessel wall. Furthermore, we addressed the impact of the inactivated fads2-locus with inactivated PUFA synthesis on the development and progression of extended atherosclerosis in two different mouse mutants with disrupted cholesterol homeostasis, using the apoe−/− and ldlr−/− mutants and the fads2−/− x apoe−/− and fads2−/− x ldlr−/− double mutants. MethodsCohorts of +/+ and fads2−/− mice underwent two long-term dietary regimens: a) a PUFA-free standard chow diet containing only EFAs, essential for viability, and b) a high fat/high cholesterol (HFHC) diet, a mimicry of the human atherogenic “Western” diet. c) To study the molecular impact of PUFA synthesis deficiency on the development and progression of atherosclerosis in the hypercholesterolemic apoe−/− and ldlr−/− mouse models fed PUFA-free regular and sustained HFHC diets, we generated the fads2−/− x apoe−/− and the fads2−/− x ldlr−/− double knockout mutants. We assessed essential molecular, biochemical and cell biological links between the diet-induced modified lipidomes of the membrane systems of the endoplasmic reticulum/Golgi complex, the site of lipid synthesis, the PL monolayer and neutral lipid core of LD and serum-Lp profiles and cellular reactions in the aortic wall. Resultsω3-and ω6-PUFA synthesis deficiency in the fads2−/− mouse causes a) hypocholesterolemia and hypotriglyceridemia, b) dyslipoproteinemia with a shift of high-density lipoprotein (HDL) to very low-density lipoprotein (VLDL)-enriched Lp-pattern and c) altered liver lipid droplet structures. d) Long-term HFHC diet does not trigger atherosclerotic plaque formation in the aortic arc, the thoracic and abdominal aorta of PUFA-deficient fads2−/− mice. Inactivation of the fads2−/− locus, abolishing systemic PUFA synthesis in the fads2−/− x apoe−/− and fads2−/− x ldlr−/− double knockout mouse lines. ConclusionsDeficiency of ω3-and ω6-PUFA in the fads2−/− mutant perturbs liver lipid metabolism, causes hypocholesterolemia and hypotriglyceridemia and renders the fads2−/− mutant resistant to sustained atherogenic HFHC diet. Neither PUFA-free regular nor long-term HFHC-diet impacts the apoe- and LDL-receptor deficiency–provoked hypercholesterolemia and atherosclerotic plaque formation, size and distribution in the aorta. Our study strongly suggests that the absence of PUFAs as highly vulnerable chemical targets of autoxidation attenuates inflammatory responses and the formation of atherosclerotic lesions.The cumulative data and insight into the molecular basis of the pleiotropic functions of PUFAs challenge a differentiated view of PUFAs as culprits or benefactors during a lifespan, pivotal for legitimate dietary recommendations.

Structure and Contact Angle in Sessile Droplets of Binary Mixtures of Lennard-Jones Chains: A Molecular Dynamics Study.

Molecular dynamics simulations were carried out to investigate cylindrical droplets consisting of binary mixtures of Lennard-Jones (LJ) fluids in contact with a solid substrate. The droplets are composed of mixtures of the monomeric LJ fluid plus linear-tangent chains of 2, 10, 20, and 30 segments per chain that interact through a harmonic potential and the spherically truncated and shifted potential Lennard-Jones. The solid surface was modeled as a semi-infinite platinum substrate with an FCC structure that interacts with the fluid by means of a LJ 9-3 potential. We place emphasis on the effect of mixing a monomeric LJ fluid with heavy components on the contact angle and on the droplet structure, especially in the liquid-solid region. The density profiles of the droplets reveal a strong discrete layering of the fluid in the vicinity of the solid-liquid interface. The layering is more pronounced at low temperatures and for mixtures of short chains (symmetric mixtures). The ordering of the fluid was much less intense for fluids of long chains (asymmetric mixtures), and some cases even show gas enrichment at the solid-liquid interface. Enrichment at the vapor-liquid interfaces and density inversion can also be observed. However, these effects are not as marked as in planar interfaces. The contact angle between the droplet and the substrate is calculated by fitting an ellipse to the vapor-liquid interface defined by the Gibbs dividing surface. In general, an increment in the concentration of the heavy component and a reduction of the temperature resulted in an increase of the contact angle, which in turn disfavored the wetting of the droplet.

The use of modified silica to control the morphology of polyamide 11 and poly(phenylene oxide) blends

Silica having amine functional groups (A-SiO2) obtained by the sol-gel process was used to improve compatibility of polyamide 11 and poly(phenylene oxide) (PA11/PPO 80/20) blend via reactive extrusion in a co-rotating twin screw extruder. Amine functional groups of A-SiO2 can react with the carboxyl groups of PA11 to form graft copolymer with PA11 which can efficiently control the phase morphology of the blend. Silica, thanks to the reinforcing effect, significantly increased stiffness of PA11/PPO blend. On the other hand, it greatly improved impact strength and reduced the crystallinity without affecting the crystallization temperature of PA11due to excellent compatibilizing effect. SEM results showed that despite the lower content of PPO, it formed a continuous phase and PA11 - a dispersed. The addition of A-SiO2 changed the morphology from the droplet-matrix to co-continuous with interpenetrating phases. The greatest size-reduction of both phases, reflecting the highest impact toughness, was observed for the content of 3 wt % A-SiO2. With a higher silica loading, phase inversion was observed with the reappearance of the droplet structure, resulting in a slight decrease in impact strength and significant in elongation at break. TGA showed that the composites exhibited better thermal properties as evidenced by the higher initial degradation temperature (Tonset) and the maximum weight loss rate temperature (Tmax).

Flexible fabrication of lipophilic-hydrophilic micromotors by off-chip photopolymerization of three-phase immiscible flow induced Janus droplet templates

Self-propelled microparticles are promising for lots of applications ranging from analytical detection to water treatment. Herein, we present an effective approach to fabricate lipophilic-hydrophilic micromotors via the photocuring of three-phase immiscible flow induced droplet templates. In the microfluidic system, two immiscible inner fluids, the lipophilic 1, 6-Hexanediol diacrylate (HDDA), and the hydrophilic poly (ethylene glycol) diacrylate (PEGDA), are simultaneously injected into a theta-shaped cylindrical capillary from two separate inlets, and they are emulsified into Janus drops when encountering the outer immiscible silicone oil. Because of the immiscible feature of droplet templates, off-chip photopolymerization strategy has been used, which can significantly decrease the blocking chance of microdevice. And also, the lipophilic-hydrophilic structure of droplets is convenient for the loading of cargos with different characteristics. More importantly, the size and configuration of droplet templates can be flexibly regulated by changing the flow rates of three different phases. Accordingly, multifunctional micromotors can be fabricated by adding different nanoparticles and materials into the HDDA or PEGDA phase first and then photocuring the droplets. Taking the bubble-propelled micromotors for example, we prepare microswimmers by loading Ag, TiO2 and Fe3O4 nanoparticles into the PEGDA phase. The swimming behaviors of micromotors in H2O2 solution are systematically investigated, finding that the proportion of PEGDA phase and the concentration of H2O2 both positively affect the moving speed. Furthermore, the applicability of motor particles on water treatment is successfully demonstrated by using neutral red solution as the model pollutant. And the micromotors can be recycled using magnets after the catalytic degradation process. Therefore, this micromotor generation technique and this kind of micromotor can be attractive for many applications.

Roles of the Coupling Agent and Surfactant in Droplet Structure in Sizing Emulsions: A Molecular Dynamics Simulations Study

Sizing emulsions used as glass fiber surface treatments in composites manufacturing are aqueous suspensions of hydrophobic film formers, surface coupling agents, and surfactants. We employ all-atom molecular dynamics simulations to characterize droplet structures in several aqueous blends of the film-former diglycidyl ether of bisphenol A, coupling agent glycidoxypropyl trimethoxysilane, and a triblock copolymer surfactant (Pluronic L35 PEO/PPO copolymer). We show that the quasi-equilibrium states of emulsion droplets are invariant to different initial configurations. We examine the role of the surfactant in determining coupling agent partitioning between the droplet shell and corona and coupling agent cluster size distributions. This work takes a step toward systematic understanding of the sizing chemistry to optimize the interface between the glass and the resin in commercially relevant composites.

Comparison of silver carp fin gelatins extracted by three types of methods: Molecular characteristics, structure, function, and pickering emulsion stabilization

High value-added utilization of different aquatic by-products is an increasingly urgent issue in aquatic science and industry. In this work, the effects of extraction methods on the molecular characteristics, structural properties, functional properties, and Pickering emulsion stabilization behaviors of silver carp fin gelatins were comprehensively studied. All the results showed molecular characteristics of silver carp fin gelatin was the key parameter to determine their functional properties such as wide gel strength range, excellent water-holding capacity, and excellent Pickering emulsion stabilization ability. The Pickering emulsion stabilization mechanisms of fin gelatins involved an “extraction method – protein molecular characteristics – fat-binding capacity – droplet structure – water phase properties – Pickering emulsion stability” route. This work could be helpful to understand the basic information on how the molecular characteristics determine the functions of gelatins. It would be also useful for the high value-added utilization of aquatic by-products and gelatins.

Water-in-Water Emulsions, Ultralow Interfacial Tension, and Biolubrication

Water-in-Water Emulsions, Ultralow Interfacial Tension, and Biolubrication

Molecular dynamics study on emulsified oil droplets with nonionic surfactants

The stability of heavy crude oil is closely related to the netlike structure formed by the aggregation of asphaltene and resin molecules inside the oil droplets, and the aggregation is too complex to accurately obtain the microstructure of these molecules experimentally. In this paper, molecular dynamics (MD) simulation was performed to investigate the structure of emulsified oil droplets on the molecular level. The simulation results showed: (i) The stacking structure of asphaltene and resin molecules form the netlike structure of the oil droplet, and it is also considered as the main reason of high stability and viscosity of heavy crude oil. (ii) When nonionic surfactants were introduced, some of them can adsorb to the surface of oil droplet and change its hydrophilicity; others can enter the interior of the oil droplets and destroy the stacking structure of asphaltene or resins inside the oil droplet. The decreased viscosity of emulsified heavy oil in the present of surfactants attributed to the increase of hydrophilicity on the oil drop surface and the damage of stacking structure inside the oil drops. (iii) the steered molecular dynamics (SMD) simulation proved that the mechanical properties of emulsified heavy oil droplet become easier to be destroyed with the increase of surfactant concentration, and it indicates that nonionic surfactant is beneficial to promote demulsification and viscosity reduction of crude oil.

Effects of three essential oils and their nano-emulsions on Listeria monocytogenes and Shigella flexneri in Egyptian Talaga cheese

Talaga cheese is a soft Egyptian cheese that has been associated with foodborne pathogens such as Listeria monocytogenes and Shigella flexneri. Essential oils (EOs) play a pivotal role in sustainably controlling foodborne diseases and as a potential preservative in soft cheeses. However, limited data is available comparing the antibacterial activity of EOs and their nano-emulsions (NEs) when inoculated into Talaga cheese. Therefore, this study aimed to examine the antibacterial activity of carvacrol, clove, and cumin EOs, in addition to their NEs, against L. monocytogenes (NCTC 13372/ATCC® 7644) and S. flexneri (ATCC®12022TW⁎) inoculated into laboratory-manufactured Egyptian Talaga cheese during refrigerated storage. The NEs had a Z-average diameter of 32.98 ± 29.75 nm, 45.2 ± 34.25 nm, and 50.23 ± 15.7 nm and a PDI of 0.326, 0.245, and 0.307 for carvacrol, clove, and cumin NEs, respectively. The flow of active functional groups of EOs and NEs as clarified by Fourier-transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) showed the spherical-shaped droplet structure of the prepared NEs. The minimum inhibitory concentration (MIC) of all EOs and their NEs was 0.78% against L. monocytogenes and 1.56% against S. flexneri, while those of carvacrol EO and its NE were 0.78% against both microorganisms. By supplementation in cheese, NEs significantly reduced the counts of inoculated pathogens from 8.2 log10cfu/g to 1.5 log10cfu/g after 2 to 3 weeks compared to EOs, which reduced them after 4 to 5 weeks. The carvacrol NE showed excellent antibacterial activity with no cheese sensory impairment. It reduced L. monocytogenes by 99% (R%) after 7 days and after 3 weeks for S. flexneri at 0.78% concentration, while higher concentrations and a longer period were required for the other NEs to show an inhibitory effect. NEs showed a greater antimicrobial effect than their non-emulsified counterparts, especially when interacting with food items, and carvacrol NE at a low concentration (0.78%) demonstrated its efficacy as an antibacterial and natural food preservative.