Fluid Droplets Research Articles

Binary collision dynamics of immiscible Newtonian and non-Newtonian fluid droplets

This experimental and theoretical study is devoted to the investigation of head-on collisions of two immiscible Newtonian and non-Newtonian droplets. The density of the two droplets is similar, and the viscosity of 0.3% carboxymethyl cellulose droplet is slightly larger than 10 cSt silicone oil. The sizes and relative velocity of the colliding droplets close to the point of impact are measured by means of image processing. The deformed states after the impact and their evolution with time are studied by experimental visualization and the energy evolution with time are discussed by numerical results. The accuracy of the two-dimensional axisymmetric three-phase flow computational model is validated. We study the effects of collisions of non-Newtonian droplets with Newtonian droplets and the subsequent retraction kinetics. Droplet “cannibalization” is commonly observed: after collision and spreading, the droplet retracts rapidly, resulting in a Newtonian droplet wrapping around a non-Newtonian droplet. We show the whole process of droplet collision captured by a high-speed camera and obtain the cloud and velocity vector maps of the droplets by numerical simulation. The droplet wrapping phenomenon is produced by different three-phase interfacial tensions and viscosities. We delineate the different phases of the collision process and discuss the dominant forces in each phase. We calculate the energy evolution of the spreading phase and use it to derive a predictive model for the dimensionless maximum spreading diameter and spreading time.

Molecular-Level Investigation of Binary Fluid Droplets Impacting Surfaces

Droplets impacting with surfaces are commonly encountered processes in the field of protective coatings. The behavior of a colliding binary liquid droplet is sensitive to the impact velocity, surface wetting properties, and the droplet composition. Modeling molecular dynamics and classical density functional theory studies of impacting droplets as well as interfacial-surface free energies was reported on. The presence of two components in the liquid drop makes the surface collision a complicated problem. During the collision the kinetic energy of the drop is converted into heat. Thus, the temperature varies during the collision and throughout the droplet. Two extreme situations were captured by performing both adiabatic and isothermal simulations. Molecular dynamics and classical density functional theory were used to explore the effects of the mixing parameter on the phase diagram of the binary AB mixed droplets. The location of liquid–vapor and liquid–liquid phase separation was determined. In addition, the value of the interfacial tensions of all interfaces was computed. These can be used to predict when an A-rich and B-rich droplet will stay attached and when it will detach.

Oviduct epithelial spheroids during in vitro culture of bovine embryos mitigate oxidative stress, improve blastocyst quality and change the embryonic transcriptome

BackgroundIn vitro embryo production is increasingly used for genetic improvement in cattle but bypasses the oviduct environment and exposes the embryos to oxidative stress with deleterious effects on further development. Here we aimed to examine the effect of oviduct epithelial spheroids (OES) on embryo development and quality in terms of morphology and gene expression during two co-culture times (4 days: up to embryonic genome activation at 8–16 cell stage vs. 7 days: up to blastocyst stage) and under two oxygen levels (5% vs. 20%).MethodsBovine presumptive zygotes produced by in vitro fertilization (day 0) using in-vitro matured oocytes were cultured in droplets of synthetic oviductal fluid (SOF) medium with or without (controls) OES for 4 or 7 days under 5% or 20% oxygen (4 treated and 2 control groups). Cleavage rates were evaluated on day 2 and blastocyst rates on days 7–8. Expanded blastocysts on days 7–8 were evaluated for total cell numbers and gene expression analysis by RNA-sequencing.ResultsUnder 20% oxygen, blastocyst rates and total cell numbers were significantly higher in the presence of OES for 4 and 7 days compared to controls (P < 0.05), with no difference according to the co-culture time. Under 5% oxygen, the presence of OES did not affect blastocyst rates but increased the number of cells per blastocyst after 7 days of co-culture (P < 0.05). Both oxygen level and OES co-culture had a significant impact on the embryonic transcriptome. The highest number of differentially expressed genes (DEGs) was identified after 7 days of co-culture under 20% oxygen. DEGs were involved in a wide range of functions, including lipid metabolism, membrane organization, response to external signals, early embryo development, and transport of small molecules among the most significantly impacted.ConclusionOES had beneficial effects on embryo development and quality under both 5% and 20% oxygen, mitigating oxidative stress. Stronger effects on embryo quality and transcriptome were obtained after 7 than 4 days of co-culture. This study shows the impact of OES on embryo development and reveals potential molecular targets of OES-embryo dialog involved in response to stress and early embryonic development.

Topological transport of a classical droplet in a lattice of time

AbstractThouless charge pumps are quantum mechanical devices whose operation relies on topology. They provide the means for transporting quantum matter in space lattices with a single quantum precision. Contrasting space crystals that spontaneously break a continuous spatial translation symmetry and form crystals in space, time crystals have emerged as novel states of matter that organise into time lattices and spontaneously break a discrete time translation symmetry. The utility of Thouless pumps that enable topologically protected quantised transport of electrons and neutral atoms in spatial superlattices leads to the question if corresponding devices exist for time crystals. Here we show that topological pumps can be realised for time solids by transporting droplets of a liquid forward and backward in time lattices and we measure the topological index that characterises such pumping processes. By exploiting a synthetic time dimension, classical time crystals can circumvent the quantum tunnelling that underpins Thouless charge pumps. Our results establish topological pumping through time instead of space and pave the way for applications of time crystals.Key Points Periodically driven fluid droplets can be made to bounce persistently in a gravitational field and may undergo spontaneous time translation symmetry breaking forming classical time crystals. These classical time crystals can be pumped from one time site to another, and this pumping process is characterised by a topological index. Our observations establish a classical temporal counterpart to Thouless pumping of quantum particles in space lattices.

Frozen Patterns in Viscoelastic Droplets Impacting on a Subcooled Surface.

The impact of droplets on a cold surface is ubiquitous in nature and various industrial applications, ranging from the icing of supercooled droplets on aircraft to the solidification of ink droplets in 3D printing. However, our understanding of the impact dynamics of droplets of complex fluids on cold surfaces is still very limited. Here, we experimentally study the spreading and frozen patterns of viscoelastic polymer droplets falling onto a subcooled substrate. We observe that the maximum spreading diameter of post-impact droplets decreases with increasing the subcooling temperature and the polymer concentration. Remarkably, all experimental data for spreading collapse into a universal curve, following the classic theory that accounts for inertial, capillary, and viscous forces. Unexpectedly, we find that, in contrast to the case of pure fluids, which exhibits three frozen modes, only two distinct modes, namely, freezing and hierarchical cracking, can be observed for polymer droplets. Finally, based on the undercooling temperature and polymer concentration, we construct a phase diagram for characterizing the morphologies of all frozen patterns. We expect that our findings may have implications in understanding the solidification of complex fluids on cold surfaces, for instance, in the fields of spray coating, inkjet printing, and additive manufacturing.

Fluid-mediated impact of soft solids

A viscous, lubrication-like response can be triggered in a thin film of fluid squeezed between a rigid flat surface and the tip of an incoming projectile. We develop a scaling for this viscous approach stage of fluid-mediated normal impact, applicable to soft impactors. Under the assumption of mediating fluid being incompressible, the impacting solid displays two limit regimes: one dominated by elasticity, and the other by inertia. The transition between the two is predicted by a dimensionless parameter, which can be interpreted as the ratio between two time scales that are the time that it takes for the surface waves to warn the leading edge of the impactor of the forthcoming impact, and the characteristic duration of the final viscous phase of the approach. Additionally, we elucidate why nearly incompressible solids feature (a) substantial ‘gliding’ prior to contact at the transition between regimes, (b) the largest size of entrapped bubble between the deformed tip of the impactor and the flat surface, and (c) a sudden drop in entrapped bubble radius past the transition between regimes. Finally, we argue that the above time scale ratio (a dimensionless number) can govern the different dynamics reported experimentally for a fluid droplet as a function of its viscosity and surface tension.

Dynamics of non-Newtonian droplets eccentrically impacting hydrophobic spherical surfaces

In this study, the dynamic behaviors of non-Newtonian fluid droplets with shear-thinning properties eccentrically impacting hydrophobic particle surfaces are investigated through a combination of numerical simulations and experiments. The simulation integrates the dynamic contact angle and a non-Newtonian fluid power-law model within the volume of fluid model framework. The effects of apparent viscosity (η), impact velocity (v0), and dimensionless eccentricity parameter (B) on the dynamic behaviors of non-Newtonian droplets are analyzed. Furthermore, the study offers insight into the progression of pressure distribution, kinetic energy, and liquid viscosity across droplets during the entire impact process. An energy balance analysis, which includes kinetic energy, surface energy, potential energy, and viscous dissipation, is employed to elucidate the fundamental physical mechanisms that govern the dynamics of eccentric impacts of non-Newtonian droplets. Finally, a model (Recr D* = −95.7 + 11 450.6e−B/0.18) is proposed to predict the adhesion or detachment of shear-thinning droplets eccentrically impacting hydrophobic particle surfaces.

Microballistics in fungi and plants

Ballistic movements in biology are powered by muscle contraction, explosive chemical reactions, the formation and collapse of gas bubbles, merger of fluid droplets, and release of hydrostatic pressure. At the macroscopic end of this kinetic carnival we find jumping fleas, violent spider jaws, shrimp claw hammers, and squirting beetles and cucumbers. The speeds are startling, but the mechanisms seem familiar because they occur on a spatial scale that overlaps with our physical experiences. We jump, albeit more slowly than fleas, for example, and it does not seem strange that seeds will spurt from a swollen cucumber when it hits the ground. Ballistics in microscopic dimensions are very different, operating in a seemingly alien world of fluid mechanics where thin air becomes soup and gravity vanishes.

Visualization study on the dynamic behavior of shear thinning droplets impacting superhydrophobic spheres

The surface and droplet characteristics significantly affect the dynamic of droplet impact on solid surfaces. In present work, a platform is utilized to experimentally study the dynamic behavior of shear thinning fluid droplets impacting superhydrophobic spheres, where the effects of different concentrations, Weber numbers (We), particle size ratios on droplets impact are studied. The results showed that on the superhydrophobic sphere, as the shear thinning droplet concentration increases, the diffusion diameter increases, and the contact time decreases. For the same curvature, the contact time between the droplet and the sphere decreases with the increase of the We. When the We reaches 75 or above, the contact time remains constant with an increase in the We. For various curvature conditions, the contact time increases as the curvature increasing. When the diameter ratio of the sphere to the droplet is D∗≥6, the maximum diffusion coefficient remains almost constant. However, when D∗<6, the maximum diffusion dimensionless diameter significantly increases as the particle size ratio decreases. More importantly, a theoretical model considering gravity effect is presented to predict the maximum dimensionless diameter of shear thinning droplets on superhydrophobic spheres according to energy conservation. The predicted results are rationally agreement with experiments with the deviation within ±10 %.

Dynamics of an acoustically levitated fluid droplet captured by a low-order immersed boundary method

We present a variant of the immersed boundary (IB) method that implements acoustic perturbation theory to model acoustically levitated fluid droplets. Instead of resolving sound waves numerically, our hybrid method solves acoustic scattering semi-analytically to obtain the corresponding time-averaged acoustic forces on the droplet. This framework allows the droplet to be simulated on inertial timescales of interest, and therefore works with much larger time steps than traditional compressible flow solvers. To benchmark this technique and demonstrate its utility, we implement the hybrid IB method for a single droplet in a standing wave. Simulated droplet shape deformations and streaming profiles agree with available theoretical predictions. Our simulations also yield insights into the streaming profiles for elliptical droplets, for which a comprehensive analytic solution does not yet exist.

Capillary wave-assisted collapse of non-Newtonian droplets

Understanding the peripheral capillary wave propagation during droplet impact is crucial for comprehending the physics of wetting onset and droplet fragmentation. Although Newtonian droplets have been extensively studied, we show how capillary waves deform non-Newtonian droplets in such a way that rheological features, such as the critical concentrations for the overlap (c*) and entangled polymer molecules (c**), may be directly obtained from the deformation history. Determining these critical concentrations is essential as they mark transitions in the rheological behavior of aqueous polymeric solutions, influencing viscosity, elasticity, and associated fluid dynamics. We have also compared capillary waves among Newtonian, shear-thinning, and Boger fluid droplets and found that although the fluid kinematics appear to be purely biaxial extensional flow, the infinite-shear properties of the droplets dominate the physics of capillary wave formation and propagation.

Dynamics of the Shape of Magnetic Fluid Droplets Under the Influence of a Magnetic Field: Experiment and Lattice Boltzmann Simulation

Dynamics of the Shape of Magnetic Fluid Droplets Under the Influence of a Magnetic Field: Experiment and Lattice Boltzmann Simulation

Wetting and pressure gradient performance in a lattice Boltzmann color gradient model

An accurate implementation of wetting and pressure drop is crucial to correctly reproduce fluid displacement processes in porous media. Although several strategies have been proposed in the literature, a systematic comparison of them is needed to determine the most suitable for practical applications. Here, we carried out numerical simulations to investigate the performance of two widely used wettability schemes in the lattice Boltzmann color gradient model, namely, the geometrical wetting scheme by Leclaire et al. [Phys. Rev. E 95(3), 033306 (2017)](scheme-I) and the modified direction of the color gradient scheme by Akai et al. [Adv. Water Resour. 116, 56–66 (2018)] (scheme-II). We showed that scheme-II was more accurate in simulating static contact angles of a fluid droplet on a solid surface. However, scheme-I was more accurate in simulating a dynamic case of a binary fluid flow in a horizontal capillary tube described by the Washburn equation. Moreover, we investigated the performance of two popular pressure gradient implementation types. Type-I used the so-called Zou–He pressure boundary conditions at the inlet and the outlet of the domain, while type-II used an external body force as a pressure gradient. We showed that the type-I implementation was slightly more accurate in simulating a neutrally wetting fluid in a horizontal capillary tube described by the Washburn equation. We also investigated the differences between the two types of pressure gradient implementation in simulating two fluid displacement processes in a Bentheimer sandstone rock sample: the primary drainage and the imbibition displacement processes.

Hypergolic fuel impacting a gelled oxidizer wall: Droplet dynamics, heat release, ignition, and flame analysis

The combination of high-concentration solutions of hydrogen peroxide, known as High Test Peroxide (HTP), with the green fuel composed of tetramethylethylenediamine, dimethylaminoethanol, and methanol (TMEDA/DMEA/MeOH, 1:1:1 vol%), catalyzed by 1 wt% of copper chloride dihydrate, has significant potential for space hypergolic propulsion applications in terms of performance and safe operation. Besides the well-known and mature propulsion systems adopting liquid and solid propellants, gel propellants also present interesting characteristics that can be better explored to enable the creation of alternative propulsive systems. The present work employed HTP at 98 wt% with 6 wt% of fumed silica as a gelling agent to create gelled HTP (GHTP). Droplets of the green fuel impinged on a layer of GHTP placed over a glass slide, acting as a solid oxidizer, mimicked an element of a new hybrid gel/liquid hypergolic propulsion system. Data from infrared and visible light cameras enabled a detailed analysis of the dynamics of a reactive droplet impinging on a gelled oxidizer wall, as well as the heat release, ignition, and flame spread of the hypergolic GHTP/green fuel pair under open-air conditions. The heat release was observed to be distributed across concentric annular areas for both the fuel surrogate and the fuel with catalyst, before and after ignition, demonstrating a strong correlation with non-reactive droplet fluid dynamics, which was corroborated by spread diameter analysis processed from visible image data. Vapor and Ignition Delay Times (VDT and IDT) were found to be as low as 4 and 17 ms, respectively, for the highest droplet impact velocity and catalyst concentration. The reaction rate in the gas phase, considering the flame spread area, showed a dependency of both impact velocity and catalyst concentration, with the latter exhibiting a more pronounced and clear effect. The surface temperature ranges where first vaporization and ignition occurred were 60 °C ∼ 70 °C and 120 ∼ 170 °C, respectively, which is close to the boiling point of methanol and the auto-ignition temperature of TMEDA. This finding, along with the chemical mechanisms in the gas phase related to the presence of a catalyst in the fuel, may be important for hypergolic ignition. The relatively low ignition temperatures and the short ignition times represent additional advantages of the present hypergolic combination for propulsion applications.

Study on the regulation of droplet motion behavior on superhydrophobic surfaces

Wettability properties of the surface is essential for controlling the dynamic sliding of droplets, thereby facilitating surface self-cleaning and drag reduction. This study makes use of chemical etching and surface modification techniques to prepare superhydrophobic surfaces with micro-nano pore structures on copper. This research examines and evaluates the chemical composition, surface morphology, and hydrophobic characteristics of superhydrophobic surfaces, also investigates the exhibited excellent superhydrophobic properties, a positive correlation observed between the concentration of the modifier solution and the surface’s hydrophobic strength. Droplets move in an accelerated fashion on superhydrophobic surfaces, and the total resistance to droplet sliding decreases as the surface hydrophobicity increases and the wall tilt angle decreases. During the motion of droplets, the fluctuation range of the receding angle is considerably greater than that of the advancing angle, thereby rendering the receding angle as the primary determinant factor for droplet movement, and as the hydrophobicity of the surface improves, the fluctuation extent of the droplet’s receding angle experiences a significant diminution. Droplet motion on superhydrophobic surfaces comprises both slipping and rolling behaviors, the increased hydrophobicity of the surface and the larger tilt angle facilitate droplet movement in a slipping mode on the surface, within the experimental parameters, the proportion of rolling distance to slipping distance varies from 35 % to 15 %. This study has revealed innovative regulatory approaches for modulating the moving behavior of liquids on microstructured surfaces, potentially enabling the development of superior functional surfaces for fluid drag reduction or droplet manipulation in the future.

Super anti‐impact silicone elastomer with shell‐less shear thickening fluid droplets in matrix

AbstractA soft and stretchable safeguarding silicone rubber is obtained by embedding large amount of shell‐less shear thickening fluid (STF) droplets into polydimethylsiloxane (PDMS) matrix through the synergistic process of emulsification and polymerization. Feeding time and temperature are optimized to generate the efficient STFs with 72.2 wt% SiO2 and high thickening viscosity of 6340 Pa·s. The embedding process and the evolution of the shell‐less STF droplets in PDMS matrix are explored by both optical and scanning electronic microscopes. The influence of the STF content on the mechanical and anti‐impact performance of the composites is explored through tensile tests and drop hammer tests. The STF content could be as much as 75 wt%, which enhances not only the softness and stretchability (elongation at break &gt;200%) but also the impact‐protective performance of the silicone rubber. The STF/PDMS composites covering on planar and nonplanar glass substrates demonstrate self‐adaptive protection effect by reducing the force impact peak by 35%, due to the shear thickening and energy absorption of STF in the composites. This study develops a facile and scalable method for the implantation of the STF into polymer matrix, extending the applications of STF/PDMS composites in varied fields.

Inkjet-Assisted Electroforming of Magnetically Guidable Devices for Interfacial Microfluidic Manipulation

In the last few decades, inkjet printing (IJP) evolved into a versatile manufacturing technique able to find application in a great number of industrial fields (e.g. electronics, display production, flexible devices). Thanks to its scalability and efficiency, IJP proved to be one of the most promising technologies to pattern materials on a wide variety of rigid and flexible substrates. Recently, it also found application in the field of microfabrication. It was employed, for example, to manufacture microelectromechanical systems (MEMS) [1] or microelectronic components and sensors [2]. Another possible application of IJP, still not developed in the existing literature, is the production of magnetically guidable microrobots [3]. These can find application in a wide variety of fields, including for example micromanipulation, cell transport and drug delivery applications.Many applications of IJP in microfabrication, and microrobots production in particular, require the presence of conductive or magnetic layers to allow actuation or sensing. By directly employing IJP, however, it is difficult to obtain highly conductive and mechanically stable layers. From this point of view, the variety and the performances of IJP printed conductive and magnetic materials are in many cases limited. To expand IJP applicability to micromanufacturing, we developed a novel approach to indirectly pattern bulk metallic microstructures by combining IJP printing and electroforming. Initially, a layer of SU-8 photoresist is IJP printed [4] on a conductive substrate to form a negative of the final pattern. The positive pattern is then growth by means of electrodeposition inside this negative SU-8 pattern. SU-8 is finally removed, leaving an indirectly printed metal pattern. It is also possible to dissolve the substrate in a suitable aggressive solution, leaving thus freestanding electroformed planar structures.In the present work, the technique described is applied to the microfabrication of untethered functional microdevices inspired to the shape and behavior of water-striders (Gerridae) [5]. These insects exploit water surface tension to walk on the surface of shallow lakes and ponds. Taking inspiration from Gerridae, micromachined devices able to move on the air-water interface were developed. A stack of three metallic layers (Cu, NiFe, Cu) was electrodeposited inside a SU-8 pattern. The substrate was subsequently dissolved, releasing thus the electroformed microdevices. These were actuated by applying a controlled magnetic field gradient and they proved able to manipulate droplets of fluids floating at the air-water interface. Moreover, the possibility to carry out microreactions by merging droplets in a controlled way was demonstrated as well.[1] R. Bernasconi et al., Micromachines 14, 2082 (2023)[2] N. K. Mani et al., “Bioelectrochemical Interface Engineering”, chapter 19, John Wiley & Sons (2020)[3] S. Palagi et al., Nat. Rev. Mater. 3(6), 113 (2018)[4] R. Bernasconi et al., Polymer 185, 121933 (2019)[5] R. Bernasconi et al., ACS Appl. Mater. Interfaces 15, 2396-2408 (2023)

Droplet Microfluidics for High-Throughput Screening and Directed Evolution of Biomolecules.

Directed evolution is a powerful technique for creating biomolecules such as proteins and nucleic acids with tailor-made properties for therapeutic and industrial applications by mimicking the natural evolution processes in the laboratory. Droplet microfluidics improved classical directed evolution by enabling time-consuming and laborious steps in this iterative process to be performed within monodispersed droplets in a highly controlled and automated manner. Droplet microfluidic chips can generate, manipulate, and sort individual droplets at kilohertz rates in a user-defined microchannel geometry, allowing new strategies for high-throughput screening and evolution of biomolecules. In this review, we discuss directed evolution studies in which droplet-based microfluidic systems were used to screen and improve the functional properties of biomolecules. We provide a systematic overview of basic on-chip fluidic operations, including reagent mixing by merging continuous fluid streams and droplet pairs, reagent addition by picoinjection, droplet generation, droplet incubation in delay lines, chambers and hydrodynamic traps, and droplet sorting techniques. Various microfluidic strategies for directed evolution using single and multiple emulsions and biomimetic materials (giant lipid vesicles, microgels, and microcapsules) are highlighted. Completely cell-free microfluidic-assisted in vitro compartmentalization methods that eliminate the need to clone DNA into cells after each round of mutagenesis are also presented.

Does dispersed phase inertia affect the shape of sheared emulsion droplets?

Inertial effects on sheared emulsion droplets are a topic of scientific and industrial interest for several applications from processing to microfluidics. Most of the literature have addressed so far the role of inertia of the continuous phase, which is known to affect shear-induced droplet deformation and migration at values of the Reynolds number of the external fluid Rec &amp;gt; 1. However, less attention has been paid to the case of inertial effects inside the droplets, corresponding to values of the Reynolds number of the droplet fluid Red &amp;gt; 1. Such a case is especially relevant when the viscosity ratio λ between the droplet and the external fluid is ≪ 1, which is typical of water-in-oil emulsions where the low values of droplet viscosity can result in Red &amp;gt; 1, while Rec &amp;lt; 1 due to the larger oil viscosity. Here, we focus on the effect of droplet inertia under shear flow at λ ≪ 1 by high-speed video microscopy experiments in a microcapillary and by numerical simulations based on a front-tracking finite-difference method. The results unveil the droplet's three-dimensional shape under shear flow at low viscosity ratios and show that droplet inertia tends to increase droplet deformation and orientation along the flow direction and to form two vortices inside the droplets even at small Rec. The latter findings are at variance with the case of external fluid inertia, where droplets become more aligned with the velocity gradient direction.

Weather-related changes in the dehydration of respiratory droplets on surfaces bolster bacterial endurance

HypothesisThe study shows for the first time a fivefold difference in the survivability of the bacterium Pseudomonas Aeruginosa (PA) in a realistic respiratory fluid droplet on fomites undergoing drying at different environmental conditions. For instance, in 2023, the annual average outdoor relative humidity (RH) and temperature in London (UK) is 71 % and 11 °C, whereas in New Delhi (India), it is 45 % and 26 °C, showing that disease spread from fomites could have a demographic dependence. Respiratory fluid droplet ejections containing pathogens on inanimate surfaces are crucial in disease spread, especially in nosocomial settings. However, the interplay between evaporation dynamics, internal fluid flow and precipitation and their collective influence on the distribution and survivability of pathogens at different environmental conditions are less known. ExperimentsShadowgraphy imaging is employed to study evaporation, and optical microscopy imaging is used for precipitation dynamics. Micro-particle image velocimetry (MicroPIV) measurements reveal the internal flow dynamics. Confocal imaging of fluorescently labelled PA elucidates the bacterial distribution within the deposits. FindingsThe study finds that the evaporation rate is drastically impeded during drying at elevated solutal concentrations, particularly at high RH and low temperature conditions. MicroPIV shows reduced internal flow under high RH and low temperature (low evaporation rate) conditions. Evaporation rate influences crystal growth, with delayed efflorescence and extending crystallization times. PA forms denser peripheral arrangements under high evaporation rates and shows a fivefold increase in survivability under low evaporation rates. These findings highlight the critical impact of environmental conditions on pathogen persistence and disease spread from inanimate surfaces.