Connected and autonomous vehicles (CAVs) and human-driven vehicles (HDVs) will inevitably coexist on roads in the future, creating mixed-flow traffic. The heterogeneous car-following (CF) behavior of HDVs can degrade the control performance of CAVs and introduce inefficiencies in CAV operations. To address these challenges, it is necessary to comprehend HDV CF behavior in mixed-flow traffic. This driving simulator-based study investigates HDV CF behavior in mixed-flow traffic under three different CAV control settings (string-stable, string-unstable, and HDV-like). The effects of traffic congestion level and demographic characteristics on CF behavior are also considered. Statistical analysis and CF model calibration, based on trajectory data collected from 72 participants in driving simulator experiments, are performed to examine the impacts of these factors on string stability, traffic efficiency, and safety. Then, online parameter estimation is conducted to illustrate the time-varying desired time headway, and sensitivity to spacing and speed variations (i.e., CF behavior evolution). Additionally, analysis of post-experiment interview results and eye-tracking data show that the string-stable CAV control setting is preferred by most HDV drivers, but can trigger driver distraction. The results also provide insights for CF behavior prediction and the optimal mixed platoon formation to enhance CAV benefits for traffic flow.
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