Drift Time Research Articles

Supercritical Fluid Chromatography Coupled with Drift Time Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry as a Tool for Lipid Characterization of HepG2 Cells

Lipidomic studies are often conducted using shotgun mass spectrometry (MS) or reversed-phase liquid chromatography coupled with MS (LC–MS). However, chromatographic separation offers several advantages such as an additional identification parameter (retention time), lower ion suppression, and separation of isobaric species. In contrast, quantification is more difficult because ion suppression is not the same over the whole analysis, and as a consequence more standards are needed to compensate for this. Supercritical fluid chromatography (SFC) offers orthogonal separation compared to reversed-phase LC. While the separation of lipids in reversed-phase LC is mainly based on the length of the carbon chain and the number of double bonds, lipids in SFC are mainly separated according to their lipid classes, which simplifies quantification with standards. In this study, SFC coupled with drift time ion mobility quadrupole time-of-flight mass spectrometry (DTIMS-QTOF-MS)was used to characterize the HepG2 lipidome.

Species-level discrimination of microorganisms by high-resolution paper spray – Ion mobility – Mass spectrometry

There is a projection of 10 million deaths per year by 2050 due to antimicrobial resistance, highlighting the need for rapid detection and accurate identification of microorganisms. Herein, we used the paper spray technique coupled with ion mobility separation and tandem mass spectrometry (PS-IM-MS/MS) to rapidly discriminate and identify five Bacillus species in 2 min of analysis time after only 4 h of incubation time. Bacterial cells were harvested by filtering their liquid cultures and ionized directly by PS. Numerical multivariate statistics (principal component analysis, followed by linear discriminant analysis) allowed species-level discrimination with a prediction rate of 92.4% and 97.6% utilizing the negative and positive ion information of PS-MS/MS, respectively. However, upon including the biomarkers' corresponding drift times, i.e., PS-IM-MS/MS, prediction rates of ca. 99.7% and 100% were obtained utilizing the negative and positive ion information, respectively. We attributed the enhancement in the prediction rates to the capability of IM separations in resolving isomers. Surfactins and phospholipids were the major ion populations detected in our experiments. They were found to have various diagnostic isomers for various Bacillus species. Therefore, improved prediction rates could be obtained upon including high-resolution IM separations in the workflow. These proof-of-concept studies described here suggest that PS-IM-MS/MS workflow would enable rapid discrimination of closely related bacteria species and strains.

Advancements in modeling conformally doped X-MSND radiation imagers

A novel bimodal radiation imager (X-ray and thermal neutron) was designed using a fin–trench microstructured silicon diode backfilled with 6LiF, then bonded to a Timepix pixelated imaging ASIC (X-MSNDs). A simulation scheme had been devised which combined finite element analysis and discrete charge carrier transport to simulate the imaging characteristics of such arbitrarily doped 3D silicon Timepix sensors. However, the precise dopant profile of the deeply etched silicon surface that forms the X-MSND diode junction is difficult to measure with conventional optical or chemical staining means. A set of boundary conditions was instead derived from simple particle transport simulations and the constant-surface-concentration silicon diffusion model. Finite element analysis of the microstructured devices using COMSOL Multiphysics and this derived boundary condition revealed that the fin region of the sensors was generally un-depleted under the maximum 300V reverse bias, and the calculated electric field along the thickness of the sensor in the microstructured region is negligible. Charge carrier transport simulations using Allpix2 predicted charge carrier motion in the fin region is dominated by slow diffusion along the fin dimension, resulting in mean charge drift times of 1000ns to collect 95% of charge carriers. Neutron transport simulations indicated that single neutron detection events resulted in highly elongated pixel clusters, typically 1-pixel wide by 6-pixels along the fin dimension. Measured data collected from KSU fabricated X-MSND imagers matched these simulation results.

Two-Dimensional FAIMS-IMS Characterization of Peptide Conformers with Resolution Exceeding 1000.

A high-performance field asymmetric waveform ion mobility spectrometry (FAIMS)-IMS-MS platform was developed and applied to explore the conformational diversity of the singly and doubly charged bradykinin (BK + H+)+ and (BK + 2H+)2+ ions. With pure N2 as the FAIMS carrier gas, more than ten conformers of (BK + H+)+ can be resolved using FAIMS-IMS, as compared to only four conformers resolved using either FAIMS or IMS alone. Interestingly, multiple conformers of (BK + H+)+ were found to have completely different values of FAIMS compensation voltage (CV), while their IMS drift times were essentially the same, which were also proven experimentally to not result from the structural annealing by the collisional heating in the ion funnel. The separations in the FAIMS and IMS dimensions are substantially orthogonal, and the overall resolving power of two-dimensional FAIMS-IMS separation is largely proportional to the product of the separation resolving powers of FAIMS and IMS. Using a gas mixture of N2/He to further improve the resolving power of the FAIMS separation, the total resolving powers of the combined FAIMS and IMS separation were estimated to be about 1020 and 1400 for (BK + H+)+ and (BK + 2H+)2+ ions, respectively, which are significantly higher than the resolving power of any ion mobility-based separation techniques demonstrated so far. The combined FAIMS-IMS can thus be a much more powerful technique to explore the structural diversity of biomolecules.

The influence of ultrasonic waves on the characteristics of the radiation detector

The paper presents the results of a study of the field dependences of charge losses, silicon diffusion detectors that allow identifying single atoms and their clusters acting as charge carrier capture centers. In electric fields E > 1470 V/cm, there is an effective decrease in the capture processes, which manifests itself in the form of straightening of the field dependences of charge losses and in the narrowing of spectral lines. Effects - an ultrasonic field with an intensity of I * = 0.4 W / cm2 and a frequency of f = 15 MHz, on samples for a time t £ 45 minutes, leads to an increase in the amplitude of the signal. It is established that ultrasonic exposure leads to a decrease in charge loss, drift time and an improvement in the energy resolution of detectors. It should also be noted that fluctuations of the impurity relief in the Si-p-type volume are smoothed out after low-power ultrasonic waves pass through it for a long time, as evidenced by a significant decrease in the effective size of local defect clusters.

Physicochemical property evaluation of modified oligonucleotides by traveling-wave ion mobility mass spectrometry.

Therapeutic oligonucleotides have molecular weights of more than 6000 Da. They typically contain chemically modified structures such as phosphorothioate (PS) and a locked nucleic acid (LNA). To determine the effect of the length and chemical modification on the physicochemical properties, various nucleic acids with different lengths and modified structures were analyzed using traveling-wave ion mobility mass spectrometry (TWIMS). The physicochemical characteristics of the modified oligonucleotides were determined using IM-MS. Each oligonucleotide was evaluated by confirming the multivalent charge state drift times, collision cross-section (CCS) values, and CCS widths. By plotting the m/z for oligonucleotides of different lengths and the CCS values at each charge state, a bottoming-out shape plot at one charge per 4.0-3.5 bases was confirmed. Moreover, significant differences were observed in the CCS values between the PS-modified and unmodified oligonucleotides. The PS-modified oligonucleotide showed a wider CCS range that was proportional to the PS modification ratio of the oligonucleotide sequence. The TWIMS results showed a correlation between the length and modification of oligonucleotides and the CCS values. In addition, it suggested that each charge state of the oligonucleotide ion has different physicochemical properties.

High Kinetic Energy Ion Mobility Spectrometry – Mass Spectrometry investigations of four inhalation anaesthetics: isoflurane, enflurane, sevoflurane and desflurane

Here we report the first High Kinetic Energy-Ion Mobility Spectrometry-Mass Spectrometric (HiKE-IMS-MS) investigations involving four fluranes; isoflurane, enflurane, sevoflurane and desflurane. Unlike standard (atmospheric pressure) IMS, HiKE-IMS can detect these compounds in positive ion mode. This is because its low-pressure environment (∼ 14 mbar) and the associated short ion drift times in the HiKE-IMS ensure the reagent ions O2+• and H3O+ are present in the reaction region, and these can react with the fluranes by dissociative charge and proton transfer, respectively. However, their ion intensities are very dependent on the value of the reduced electric field (E/N) applied and the humidity of the air in the reaction region of the HiKE-IMS. In this paper we explore the potential use of HiKE-IMS for air quality control and breath analysis of fluranes. To help in the interpretation of the ion mobility spectra, and hence the ion-flurane chemistry occurring in reaction region, a HiKE-IMS was coupled to a Time-of-Flight Mass Spectrometer so that the m/z values of both the reagent and product ions that are contained within the various ion mobility peaks observed could be identified with a high level of confidence. The dependencies of the intensities of these ions as functions of E/N (30–115 Td) and humidity in the reaction region are reported. A number of product ions have been observed only under low humidity conditions (H2O volume-mixing ratio ∼ 100 ppmv), including CHF2+ and CHFCl + for isoflurane and enflurane, CHF2+, CF3+ and C3H2F5O+ for desflurane, and CH3O+, CHF2+, C3H3F4O+, C4H3F6O+ and C4H3F6O+(H2O) for sevoflurane. It is interesting to note that CH3O+, CHF2+, CHFCl+ and CF3+ have shorter drift times than that measured for O2+•. This is explained by resonant charge transfer reaction processes occurring in the drift region: O2+• + O2 ⇌ O2+•.O2 ⇌ O2 + O2+•.

Enhanced Top-Down Protein Characterization with Electron Capture Dissociation and Cyclic Ion Mobility Spectrometry.

Tandem mass spectrometry of denatured, multiply charged high mass protein precursor ions yield extremely dense spectra with hundreds of broad and overlapping product ion isotopic distributions of differing charge states that yield an elevated baseline of unresolved “noise” centered about the precursor ion. Development of mass analyzers and signal processing methods to increase mass resolving power and manipulation of precursor and product ion charge through solution additives or ion–ion reactions have been thoroughly explored as solutions to spectral congestion. Here, we demonstrate the utility of electron capture dissociation (ECD) coupled with high-resolution cyclic ion mobility spectrometry (cIMS) to greatly increase top-down protein characterization capabilities. Congestion of protein ECD spectra was reduced using cIMS of the ECD product ions and “mobility fractions”, that is, extracted mass spectra for segments of the 2D mobiligram (m/z versus drift time). For small proteins, such as ubiquitin (8.6 kDa), where mass resolving power was not the limiting factor for characterization, pre-IMS ECD and mobility fractions did not significantly increase protein sequence coverage, but an increase in the number of identified product ions was observed. However, a dramatic increase in performance, measured by protein sequence coverage, was observed for larger and more highly charged species, such as the +35 charge state of carbonic anhydrase (29 kDa). Pre-IMS ECD combined with mobility fractions yielded a 135% increase in the number of annotated isotope clusters and a 75% increase in unique product ions compared to processing without using the IMS dimension. These results yielded 89% sequence coverage for carbonic anhydrase.

North Equatorial Current and Kuroshio velocity variations affect body length and distribution of the Japanese eel Anguilla japonica in Taiwan and Japan

The larval stage of Japanese eel travels a substantial distance over a long duration through the North Equatorial Current (NEC) and the Kuroshio, and the spawning behavior of mature eels leads to monthly arrival waves in eastern Taiwan between November and February. The total length (TL) of the glass eel relates to its larval duration and age; therefore, the TL can indicate the larval duration. The monthly mean TLs of eels along eastern Taiwan from 2010 to 2021 were used to estimate the batch age, and the recruitment patterns and relative abundances were compared. The TLs of glass eels followed a normal distribution, and the estimated ages were highly correlated with their mean TLs. Early recruit TLs were significantly greater than those of late recruits. The mean tracer drift time was longer in early recruitment months (November–December) than in later dates (February–March). The recruitment lag between Taiwan and Japan was approximately 1–1.5 months, with relative more abundance in Taiwan for the early recruits and in Japan for the late recruits. Speculated cohorts followed the main streams of the NEC and Kuroshio, and the monthly velocity changes of these currents could affect the mean TLs as well as the distribution patterns of Japanese glass eels in Taiwan and Japan.

Simultaneous scintillation light and charge readout of a pure argon filled Spherical Proportional Counter

The possible use of a Spherical Proportional Counter for the search of neutrinoless double beta decay is investigated in the R2D2 R&D project. Dual charge and scintillation light readout may improve the detector performance. Tests were carried out with pure argon at 1.1 bar using a 6 × 6 mm2 silicon photomultiplier. Scintillation light was used for the first time to trigger in a spherical proportional counter. The measured drift time is in excellent agreement with the expectations from simulations. Furthermore the light signal emitted during the avalanche development exhibits features that could be exploited for event characterization.

Ion mobility spectrometry of <i>N</i>-methylimidazole and possibilities of its determination

Objectives. To determine the ion mobility of N-methylimidazole, establish the structure of ions corresponding to characteristic signals, and determine the detection limit of N-methylimidazole on the ion-drift detector Kerber.Methods. Ion mobility spectrometry was used to study the ionization processes. The enthalpies of the reactions of monomer and dimer ions were calculated in the ORCA 4.1.1 software by the B3LYP density functional method with a set of basic functions 6-31G (d, p).Results. The drift time and ion mobility values of N-methylimidazole were determined. A method for mathematical processing of spectra and a program for its implementation was developed. The changing peculiarities of the ion mobility spectrum during measurement at a given time were studied. According to the interpretation of the spectrum signals, the structure of the generated ions was proposed, and the enthalpies of ion formation were determined.Conclusions. The characteristic signal of the N-methylimidazole ion protonated at the nitrogen atom of the pyridine type was revealed. It was found that two signals in the ion mobility spectra of N-methylimidazole correspond to the presence of the monomer and dimer ions. The detection limit of N-methylimidazole on the ion-drift detector Kerber was determined, amounting to 3 pg.

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments.

Accurate characterization of chemical structures is important to understand their underlying biological mechanisms and functional properties. Mass spectrometry (MS) is a popular tool but is not always sufficient to completely unveil all structural features. For example, although carbohydrates are biologically relevant, their characterization is complicated by numerous levels of isomerism. Ion mobility spectrometry (IMS) is an interesting complement because it is sensitive to ion conformations and, thus, to isomerism. Furthermore, recent advances have significantly improved the technique: the last generation of Cyclic IMS instruments offers additional capabilities compared to linear IMS instruments, such as an increased resolving power or the possibility to perform tandem ion mobility (IMS/IMS) experiments. During IMS/IMS, an ion is selected based on its ion mobility, fragmented, and reanalyzed to obtain ion mobility information about its fragments. Recent work showed that the mobility profiles of the fragments contained in such IMS/IMS data can act as a fingerprint of a particular glycan and can be used in a molecular networking strategy to organize glycomics datasets in a structurally relevant way. The goal of this protocol is thus to describe how to generate IMS/IMS data, from sample preparation to the final Collision Cross Section (CCS) calibration of the ion mobility dimension that yields reproducible spectra. Taking the example of one representative glycan, this protocol will show how to build an IMS/IMS control sequence on a Cyclic IMS instrument, how to account for this control sequence to translate IMS arrival time into drift time (i.e., the effective separation time applied to the ions), and how to extract the relevant mobility information from the raw data. This protocol is designed to clearly explain the critical points of an IMS/IMS experiment and thus help new Cyclic IMS users perform straightforward and reproducible acquisitions.

Predicting vigilance vulnerability during 1 and 2 weeks of sleep restriction with baseline performance metrics.

We attempted to predict vigilance performance in adolescents during partial sleep deprivation using task summary metrics and drift diffusion modelling measures (DDM) derived from baseline vigilance performance. In the Need for Sleep studies, 57 adolescents (age = 15-19 years) underwent two baseline nights of 9-h time-in-bed (TIB), followed by two cycles of weekday sleep-restricted nights (5-h or 6.5-h TIB) and weekend recovery nights (9-h TIB). Vigilance was assessed daily with the Psychomotor Vigilance Task (PVT), with the number of lapses (response times ≥ 500 ms) as the primary outcome measure. The two DDM predictors were drift rate, which quantifies the speed of information accumulation and determines how quickly an individual derives a decision response, and non-decision time range, which indicates within-subject variation in physical, non-cognitive responding, e.g. motor actions. In the first week of sleep curtailment, faster accumulation of lapses was significantly associated with more lapses at baseline (p = .02), but not the two baseline DDM metrics: drift and non-decision time range (p > .07). On the other hand, faster accumulation of lapses and greater increment in reaction time variability from the first to the second week of sleep restriction were associated with lower drift (p < .007) at baseline. Among adolescents, baseline PVT lapses can predict inter-individual differences in vigilance vulnerability during 1 week of sleep restriction on weekdays, while drift more consistently predicts vulnerability during more weeks of sleep curtailment. Effects of Napping in Sleep-Restricted Adolescents, clinicaltrials.gov, NCT02838095. The Cognitive and Metabolic Effects of Sleep Restriction in Adolescents (NFS4), clinicaltrials.gov, NCT03333512.

Diagnostic ions guided 2D-locating strategy for characterization of chemical analogues in complex herbal medicines using liquid chromatography-ion mobility-mass spectrometry.

Rapid characterization of chemical analogues in potentially toxic complex matrix was essential for prevention of accidental poisoning. On the basis of the fragment ions possessed not only the same retention times (RT) but the same drift times (DT) on liquid chromatography-ion mobility-mass spectrometry (LC-IM-MS), an alternating frames (AF)-data independent acquisition (DIA) was utilized for simultaneous detection and rapid match of precursor/product ions with a fast switch of low/high collision energy (CE). A diagnostic ions guided 2D-locating strategy was developed for identification of chemical analogues in potentially toxic herbal medicines using LC-IM-MS. Firstly, the 2D-locations (RT, DT) of diagnostic ions were screened from high-fragment IM-MS frames according to their m/z. Then, the correlated precursor ions were extracted from the complex background interference in low-fragment IM-MS frames based on diagnostic ions' 2D-locations. Finally, the remained ions were characterized using double-bond equivalent analysis combined with MS/MS fragment interpretation. Totally, 236 diterpene alkaloids including eight compounds with potential new esterification types were characterized in processed lateral roots of Aconitum carmichaelii Debx. Moreover, the LC-IM-MS distribution regularities of diterpene alkaloids with various chemical structure types were further investigated and discussed. This study presented an innovative idea for revealing the chemical basis related to the toxicities of potentially poisonous herbal medicines to ensure the medication safety.

Development and Application of Multidimensional Lipid Libraries to Investigate Lipidomic Dysregulation Related to Smoke Inhalation Injury Severity.

The implication of lipid dysregulation in diseases, toxic exposure outcomes, and inflammation has brought great interest to lipidomic studies. However, lipids have proven to be analytically challenging due to their highly isomeric nature and vast concentration ranges in biological matrices. Therefore, multidimensional techniques such as those integrating liquid chromatography, ion mobility spectrometry, collision-induced dissociation, and mass spectrometry (LC-IMS-CID-MS) have been implemented to separate lipid isomers as well as provide structural information and increased identification confidence. These data sets are however extremely large and complex, resulting in challenges for data processing and annotation. Here, we have overcome these challenges by developing sample-specific multidimensional lipid libraries using the freely available software Skyline. Specifically, the human plasma library developed for this work contains over 500 unique lipids and is combined with adapted Skyline functions such as indexed retention time (iRT) for retention time prediction and IMS drift time filtering for enhanced selectivity. For comparison with other studies, this database was used to annotate LC-IMS-CID-MS data from a NIST SRM 1950 extract. The same workflow was then utilized to assess plasma and bronchoalveolar lavage fluid (BALF) samples from patients with varying degrees of smoke inhalation injury to identify lipid-based patient prognostic and diagnostic markers.

Drift Time Corrections Based on a Practical Measurement of the Depletion Zone to Allow Accurate and Reproducible Determination of the Reduced Mobility of Ions in DT-IMS.

The reduced mobility of an ion is a key parameter for identifying ions and comparing spectra in drift time ion mobility spectrometry. As the resolution of spectrometers improves, accurate determination of the reduced mobility is increasingly important. The drift time, used to calculate the reduced mobility, is affected by the ion gate, and this effect has previously been compensated with a linear correction. These corrections, however, do not allow for changes in the distances that the ions must drift to reach the detector caused by the electric field around the ion gate. As these corrections are a linear correction, nonlinearity in the influence of the ion gate may also lead to greater errors. By measuring the length of the depletion zone in front of the ion gate the extra distance traveled by the ions may be corrected for. This measurement also provides the boundary conditions for when a correction to the drift time may be accurately applied. This work shows that the length of the depletion zone can be experimentally measured and that it is consistent for a particular geometry of ion gate.

MLT‐Dependence of Sustained Spectral Gaps of Proton and Oxygen in the Inner Magnetosphere

AbstractTo examine the species dependence of the sustained energy spectrum gaps, in this letter, we analyzed the magnetic local time distribution of sustained proton and oxygen gaps and find that there is a clear difference between proton and oxygen ions: proton sustained gaps are predominantly distributed near the prenoon sector, while oxygen sustained gaps are mostly in the afternoon sector with lower occurrence rate than that for protons. This is primarily due to the different charge exchange loss rates of protons and oxygen ions. This scenario is supported by particle‐tracing simulations, which reproduce most of the observed characteristics. Through model‐data comparison, we found that the spectral gaps at different local times are formed by two different types of mechanisms: (a) the gap is formed due to the extended drift time which is responsible for the gap appearing on the dayside for both proton and oxygen ions; (b) the gap is formed inside the Alfven layer, which accounts for the observed sustained gaps in proton energy spectrums but not in oxygen ions due to the different charge exchange losses at various energies on the preexisting ion populations that lie on closed drift paths. These findings may provide insight into our understanding of ion composition and possible wave generation in the inner magnetosphere.

Study of charge carrier transport properties and lifetimes in HR GaAs:Cr with Timepix3

The response of a Timepix3 (256 × 256 pixels, pixel pitch 55 μm) detector with a 500 μm thick HR GaAs:Cr sensor was studied in proton beams of 125 MeV at the Danish Centre for Particle Therapy in Aarhus, Denmark and in a 120 GeV/c pion beam at the Super-Proton Synchrotron (SPS) at CERN. The sensor was biased at different voltages and irradiated at different angles. The readout chip was configured to operate in electron and hole collection modes. Measurements at grazing angles allowed to see elongated tracks with well-defined impact and exit points, so that charge carrier production depths could be determined in each pixel. We extracted the charge collection efficiencies and the charge carrier drift times as a function of the distance to the pixel plane. It was found that measured proton tracks are shorter in hole collection than in the case of electron collection, which is explained by the shorter lifetime of holes. At an angle of 60 degrees with respect to the sensor normal, the average track length in hole collection was ∼700 μm and 950 μm in electron collection mode. To understand the experimental findings, models describing the properties of HR GaAs:Cr were implemented into the Allpix2 simulation framework. We added previously presented experimental results describing the dependence of the electron drift velocity on the electric field and validated the response by comparing measurement and simulation for various X- and gamma-ray sources in the energy range of 10–60 keV. By comparison of the experimental and the simulated results, the mobility μ h and the lifetime of holes τ h were estimated as μ h = (320 ± 10) cm2/V/s and τ h = (4.5 ± 0.5) ns.

Frequency response and carrier escape time of InGaAs quantum well-dots photodiode

p-i-n photodiodes comprising dense arrays of InGaAs quantum dots (referred to as quantum well-dots) were fabricated, and the basic physical processes affecting their high-speed performance were studied for the first time by measuring the frequency response under illumination with photons absorbed either in the quantum well-dots (905-nm illumination) or mainly in GaAs layers (860-nm illumination). A GaAs p-i-n photodiode of similar design was also measured for comparison. A maximum −3 dB bandwidth of 8.2 GHz was measured for the 905-nm light illumination, and maximum internal −3 dB bandwidth of 12.5 GHz was estimated taking into account the effect of RC-parasitic by the equivalent circuit model. It was found that the internal response is mainly controlled by the carrier drift time in the depletion region; this process can be characterized by a field-dependent effective velocity of charge carriers in the layered heterostructure, which is approximately half the saturation velocity in GaAs. The carrier escape from the InGaAs quantum well-dots was found to has less effect; the escape time was estimated to be 12–17 ps depending on the reverse-bias voltage applied.

Picosecond electrical response in graphene/MoTe2 heterojunction with high responsivity in the near infrared region

Understanding the fundamental charge carrier dynamics is of great significance for photodetectors with both high speed and high responsivity. Devices based on two-dimensional (2D) transition metal dichalcogenides can exhibit picosecond photoresponse speed. However, 2D materials naturally have low absorption, and when increasing thickness to gain higher responsivity, the response time usually slows to nanoseconds, limiting their photodetection performance. Here, by taking time-resolved photocurrent measurements, we demonstrated that graphene/MoTe2 van der Waals heterojunctions realize a fast 10 ps photoresponse time owing to the reduced average photocurrent drift time in the heterojunction, which is fundamentally distinct from traditional Dirac semimetal photodetectors such as graphene or Cd3As2 and implies a photodetection bandwidth as wide as 100 GHz. Furthermore, we found that an additional charge carrier transport channel provided by graphene can effectively decrease the photocurrent recombination loss to the entire device, preserving a high responsivity in the near-infrared region. Our study provides a deeper understanding of the ultrafast electrical response in van der Waals heterojunctions and offers a promising approach for the realization of photodetectors with both high responsivity and ultrafast electrical response.