Reduction In Drainage Research Articles

Banana peel fermentation broth as a viable alternative carbon source for biological sulfate reduction in acid mine drainage

AbstractBACKGROUNDAcid mine drainage (AMD) is a major environmental threat in mining areas due to its strong acidity, high concentrations of sulfate, and heavy metals. Utilizing sulfate‐reducing bacteria (SRB) to reduce SO42− and remove heavy metals is a promising and economical AMD treatment. However, AMD lacks sufficient electron donors for sulfate reduction. Banana peel fermentation broth, containing various small‐molecule organic acids, is an effective alternative carbon source for SRB, promoting the use of agricultural waste in environmental remediation. This study aims to investigate the impact of adding banana peel fermentation broth to the bioreactor on pollutant removal performance and analyze its effect on the microbial community structure.RESULTSThe bioreactor utilising banana peel fermentation broth as an electron donor, demonstrated the effective removal of various pollutants, including sulfate (55.7%), Cu2+ (90.74%), Mn2+ (70.77%), Fe2+ (81.28%) and Cd2+ (100.00%), with an average reduction of 74.20% in chemical oxygen demand (COD). Sulfate reduction resulted in an increase in pH from 5.5 to 7.9. Microbial community structure analysis revealed that the primary genera involved in sulfate reduction were Desulfurispora and Desulfovibrio, while Delftia contributes to the immobilization of heavy metals. Additionally, the key genera responsible for fermentation to produce small molecule acids were Leptolinea and Sedimentibacter. In symbiosis with SRB, they play a crucial role in the removal efficiency of metals and sulfate.CONCLUSIONThe overall sulfate and heavy metals removal efficiency was found to be satisfactory after 30 days of continuous reactor operation. There was a notable increase in microbial abundance and community diversity within the reactor. Therefore, banana peel fermentation broth is a promising alternative organic carbon source in acid mine drainage(AMD) treatment. © 2025 Society of Chemical Industry (SCI).

Analysis of the efficacy of Percutaneous Transhepatic Cholangiography Drainage (PTCD) and Endoscopic Retrograde Cholangiopancreatography (ERCP) in the treatment of Malignant Obstructive Jaundice (MOJ) in palliative drainage and preoperative biliary drainage: a single-center retrospective study

PurposeThis study aimed to assess the safety and efficacy of percutaneous transhepatic cholangiography drainage (PTCD) and endoscopic retrograde cholangiopancreatography (ERCP) in palliative drainage and preoperative biliary drainage for treating malignant obstructive jaundice (MOJ).MethodsA total of 520 patients with MOJ who underwent PTCD or ERCP were enrolled and classified into palliative drainage group and preoperative biliary drainage group. Baseline characteristics, liver function, blood routine, complications were compared among the groups.ResultsThe technical success rates for PTCD and ERCP in palliative group were 97.1% and 85.9%. In palliative drainage group, PTCD had higher levels of total bilirubin (TB) reduction (53.0 (30.0,97.0) vs. 36.8 (17.9,65.0), p < 0.001) and direct bilirubin (DB) reduction (42.0 (22.0,78.5) vs. 28.0 (12.0,50.8), p = 0.001) than ERCP. However, PTCD was associated with higher rates of drainage tube displacement (20 cases, 11.8%), while ERCP had a higher incidence of biliary infection (39 cases, 22.8%) and pancreatitis (7 cases, 4.1%). In preoperative drainage group, PTCD achieved a 50% reduction in total bilirubin faster than ERCP (7.1 days vs. 10.5 days). And the time from palliation of jaundice to surgery was 24.2 days in PTCD group and 35.7 days in ERCP group, a statistically significant difference (Student’s t test, p = 0.017).ConclusionBoth PTCD and ERCP could improve liver function for MOJ patients. PTCD seems to offer better outcomes in jaundice reduction and liver function improvement in palliative drainage, but requires careful postoperative management. In preoperative biliary drainage, PTCD may be a better preoperative bridge to improve liver function and control infection.

Combining biochar with sediment in the treatment for the effectiveness of sulfate and heavy metal Pb reduction of acid mine drainage

The increasing mining activities have led to the problem of acid mine drainage (AMD) pollution. A method that combines biochar treatment as an adsorbent with wetland sediment treatment as a source of sulfate-reducing bacteria is used to address AMD effectively. This research aimed to determine the ability of biochar in combination with wetland sediment treatment to reduce sulfate and heavy metal content in acid mine drainage wastewater. This research was conducted on a laboratory scale in an AMD wastewater treatment reactor with the following treatments of biochar mixed with wetland sediment. Observations included sulfate content, pH, and heavy metal content. Scanning electron microscope (SEM) analysis was also performed on the biochar. SEM observations revealed the presence of small, dense, and irregularly shaped pores on the surface of the biochar. The results on day 30 showed that biochar mixed with wetland sediment was able to reduce sulfate concentration by 74.19% and reduced Pb by 73.79%, compared with treatment sediment only to 64.81% sulfate concentration and reduced Pb by 53.85%, treatment biochar only had reduced sulfate of 46.90% and reduced Pb by 58.67% and control 1.79% sulfate concentration and reduced Pb by 1.87%.

Contrasting effects of soil type and use of cover crops on nitrogen and phosphorus leaching in agricultural systems of the Argentinean Pampas

Agricultural systems of the Argentinean Pampas generally use rotations of summer crops under no-tillage and apply fertilization at rates that do not compensate nutrient exports with grain harvest, resulting in negative balances and soil fertility loss. However, the humid climate, long fallow periods and soil factors enhance nitrogen (N) and phosphorus (P) leaching, which has a negative impact on freshwater ecosystems. We propose to evaluate the effect of soil type and replacement of fallow periods with cover crops on drainage and N and P leaching. We used lysimeters containing two Mollisols of contrasting texture and porosity (a Typic Argiudoll and a Typic Hapludoll), and cultivated between 2014 and 2022 with a maize-soybean rotation with (M-CC-S) and without (M-S) cover crops under no tillage.The amount of drainage varied with rainfall (p<0.01), was similar between soils and higher in M-S than in M-CC-S during the intercropping period (148 vs 96 mm, p<0.05). The correlation between daily rainfall and drainage was higher for the Typic Argiudoll (r=0.46 and 0.42, for M-S and M-CC-S, respectively) than for the Typic Hapludoll (0.28 and 0.23 for M-S and M-CC-S, respectively) when considering same-day events, and it decreased for the Typic Argiudoll (r=0.31 and 0.26, for the M-S and M-CC-S, respectively) but not for the Typic Hapludoll (0.28 and 0.21 for the M-S and M-CC-S, respectively) with a 1-day lag between series. The amount of N and P lost through leaching varied with drainage (p<0.01). Cover crops reduced nitrate leaching to a greater extent in the Typic Hapludoll (from 30.4 to 3.3 kg ha−1 period−1) than in the Typic Argiudoll (from 17.6 to 2.0 kg ha−1 period−1) (p<0.05). Soil type modified P leaching in M-S (0.32 and 0.11 kg ha−1 period−1 for the Typic Argiudoll and the Typic Hapludoll, respectively) and M-CC-S had intermediate P leaching (0.17 and 0.16 kg P ha−1 period−1 for the Argiudoll and Hapludoll, respectively) (p<0.05).We conclude that: 1) water flows through different channels in each soil, the rapid flow of the Typic Argiudoll probably occurs through preferential flow channels (macropores) while the slower flow of the Typic Hapludoll is probably associated to matrix porosity, 2) P leaching is related to soil type (Typic Argiudoll > Typic Hapludoll) and drainage reduction by cover crops off-sets P leaching through macropores in the Typic Argiudoll, and 3) cover crops reduce N leaching, due to drainage reduction and N retention in cover crop biomass.

Source reduction and end treatment of acid mine drainage in closed coal mines of the Yudong River Basin.

After the closure of the Yudong coal mine, the pH value was approximately 3.0, and the Fe and Mn concentrations reached 380 and 69 mg/L, respectively, in the acid mine drainage (AMD), causing serious pollution to the water bodies in the nearby watershed. Combined with the formation conditions of AMD, the comprehensive treatment technology of source reduction-end treatment is adopted to treat the AMD. The treatment area of the goaf is 0.3 km3, the filling and grouting volume is about 6.7 m3, and the curtain grouting volume is 4,000 m3. Through the grouting and sealing treatment in the goaf, the water volume is reduced to less than 85% of the initial volume (100 m3/h). After the end treatment, the pH value of the effluent is around 7.0, the content of Fe and Mn is less than 0.1 mg/L, and the removal rate is above 99%. The project was subsequently operated at RMB 0.85 yuan/t. This project is aimed at the treatment of AMD from small coal mines in complex terrain conditions. It has the characteristics of low cost and high efficiency and can provide an effective treatment technology for AMD in southwestern China and areas with the same geological conditions.

Dural Arteriovenous Fistula Presenting as Acute Unilateral Vestibulopathy

Intracranial dural arteriovenous fistula (dAVF) is characterized by an abnormal connection between branches of arteries and veins in the dura mater. Clinical manifestations of dAVF vary depending on their location, feeder arterial supply, amount of shunting, and most importantly, their venous drainage pattern. Acute vertigo has been rarely reported as an initial presentation of dAVF due to venous congestion in the brainstem. We report a patient who presented with acute right vestibulopathy without any brainstem signs in dAVF involving the transversesigmoid sinus. The patient showed abnormal caloric response but normal head impulse in the affected ear. Without any treatment, the patient’s symptoms gradually improved with a normalization of right canal paresis. Follow-up cerebral angiography also revealed a spontaneous regression of the shunt flow and reduction of venous drainage at the right transverse-sigmoid sinus. Based on the results of vestibular function tests and cerebral angiography, acute vertigo in our patient may be ascribed to impaired reabsorption of endolymph by focal venous congestion.

Pleural drainage vs video-assisted thoracoscopic debridement in children affected by pleural empyema

BackgroundBoth thoracic drainage and video-assisted thoracic surgery (VATS) are available treatment for pleural empyema in pediatric patients.Materials and methodsThis retrospective multicenter study includes pediatric patients affected by pleural empyema treated from 2004 to 2021 at two Italian centers. Patients were divided in G1 (traditional approach) and G2 (VATS). Demographic and recovery data, laboratory tests, imaging, surgical findings, post-operative management and follow-up were analyzed.Results70 patients with a mean age of 4.8 years were included; 12 (17.1%) in G1 and 58 (82.9%) in G2. Median surgical time was 45 min in G1, 90 in G2 (p < 0.05). Mean duration of thoracic drainage was 7.3 days in G1, 6.2 in G2 (p > 0.05). Patients became afebrile after a mean of 6.4 days G1, 3.9 in G2 (p < 0.05). Mean duration of antibiotic therapy was 27.8 days in G1, 25 in G2 (p < 0.05). Mean duration of postoperative hospital stay was 16 days in G1, 12.1 in G2 (p < 0.05). There were 4 cases (33.3%) of postoperative complications in G1, 17 (29.3%) in G2 (p > 0.05). 2 (16.7%) patients of G1 needed a redosurgery with VATS, 1 (1.7%) in G2.ConclusionsVATS is an effective and safe procedure in treatment of Pleural Empyema in children: it is associated to reduction of chest tube drainage, duration of fever, hospital stay, time of antibiotic therapy and recurrence rate.

Association of the Japanese herbal kampo medicine kakkonto with antibiotic use and surgical drainage for noninfectious mastitis: A nationwide database study.

Kakkonto, a Japanese herbal kampo medicine, is empirically prescribed to improve milk stasis and ameliorate breast inflammation in patients with noninfectious mastitis. We investigated whether early use of kakkonto is associated with a reduction in antibiotic use and surgical drainage in patients with noninfectious mastitis. We identified 34 074 patients with an initial diagnosis of noninfectious mastitis within 1 year of childbirth between April 2012 and December 2022 using the nationwide administrative JMDC Claims Database. Patients were divided into the kakkonto (n = 9593) and control (n = 9648) groups if they received and did not receive kakkonto on the day of the initial diagnosis of noninfectious mastitis, respectively. Antibiotic administration and surgical drainage within 30 days after the initial diagnosis of noninfectious mastitis in the two groups were compared using propensity score-stabilized inverse probability of treatment weighting analysis. The frequency of antibiotic administration within 30 days after the initial diagnosis of noninfectious mastitis was significantly lower in the kakkonto group than in the control group (10% vs. 12%; odds ratio, 0.88 [95% confidence interval, 0.80-0.96]). The frequency of antibiotic administration during 1-3 and 4-7 days after the initial diagnosis were also significantly lower in the kakkonto group than in the control group. The frequency of surgical drainage did not differ significantly between the two groups. Kakkonto was associated with reduced administration of antibiotics for noninfectious mastitis, making it a potential treatment option for relieving breast inflammation and promoting antimicrobial stewardship.

Microbial electrolysis cell as an effective tool for mitigating the toxic effects of Cu2+ on microorganisms in the acid mine drainage treatment: Efficacy and mechanisms

It is poorly understood how the mechanism of heavy metals affect sulfate-reducing bacteria (SRB) in microbial electrolysis cell (MEC) system, however, this is essential to improve the effectiveness of sulfate reduction in acid mine drainage (AMD). This study evaluated the impact of Cu2+ on MEC performance using SRBs enriched biocathode. The sulfate reduction rate and pathway, the fate of Cu, as well as the succession of microbial community were investigated. Results showed that MEC performance was optimized at influent Cu2+ concentration of 20 mg/L, while slight inhibition was observed at 40 mg/L. The removal of Cu2+ was accomplished through bioprecipitation and biosorption, forming CuS and Cu(OH)2. However, MEC performance was inhibited at higher Cu2+ concentrations (60 mg/L) due to damaged cell structure, increased charge transfer internal resistance, and altered microbial communities. Specifically, the relative abundance of Firmicutes and Bacteroidota decreased, and functional genes related to sulfate reduction metabolism were reduced, leading to impairment of the assimilatory and dissimilatory sulfate reduction pathways. This knowledge provides revealing insights and theoretical support for developing a feasible approach to realize the treatment of wastewater containing multiple pollutants.

Rock Aggregate Size Influence on Physical Degradation of Laminate Sedimentary Rocks in Flexible Pavement

Rock aggregates are the most fundamental material of highways, railroads, and other construction activities. Degradation of aggregate is the disintegration of aggregate due to the wheel load and moisture saturation causing loss of support in unbound granular layer and reduction of drainage in pavement materials. Sedimentary rocks from different quarries were evaluated to determine the influence of aggregate size on the degradation of materials. This study is to find out the effect of size on physical parameters of aggregate used in flexible and rigid pavement. The performance of river rock and crushed rock physical properties has been tested and compared. In order to determine the effect of aggregate size on their degradation properties, different sieve ranges were selected. The sieve sizes are graded into four categories according to Indian Standard sieve: R1 (20 mm-16 mm), R2 (16 mm-12.5 mm), R3 (12.5 mm-10 mm), and R4 (10 mm-4.75 mm) for degradation test such as, aggregate impact value (AIV), aggregate crushing value (ACV), water absorption (WA) and weathering test such as soundness and slake durability. It is observed that the larger size of aggregate has given better resistance against impact load, abrasion, weathering action and water absorption compare to smaller size over the same volume of rock. The rate at which aggregate degradation occurs is influenced by size. Along with the wheel load, environmental factors including moisture and temperature changes are the primary causes of the early degradation of aggregate. The river rock has a better resistance against degradation and weathering action as it has lesser lamination (a small scale sequence of fine rock layer). Adoption of larger size aggregate in the pavement base course is suggested to withstand the effect of environmental action and wheel load, especially on the friable and quickly disintegrated rock. Arating value, rating index and regression equation have been developed for aggregate degradation. Keywords: Particle Size, Properties of Rock, Weathering, Slake Durability, Degradation Index

Simulated impacts of winter rye cover crop on continuous corn yield and soil parameters

AbstractCover crops (CC) provide numerous ecosystem services such as improving soil health, reducing nutrient loss, increasing productivity, and mitigating greenhouse gas emission. However, adoption of CC has been hindered by perceived negative impacts on main crop productivity and additional production costs. This is partly attributed to the gap in current state of knowledge in CC and its interaction with main crop production under different biophysical conditions. In this study, Decision Support System for Agrotechnology Transfer model was used to evaluate the long‐term impact of cereal rye (Secale cereale L.) on corn (Zea mays L.) yield, soil organic carbon (SOC), nitrate leaching, soil water, and drainage for a range of climate, soil, and irrigation management in Eastern and Central Nebraska. A 30‐year (1991–2020) simulation showed no difference in corn yield and SOC between CC and no‐cover crop treatments at both sites under irrigated and rainfed conditions. However, CC resulted in reduction of N loss by up to 48% at the Eastern Nebraska Research and Extension Center and 24% at South Central Agricultural Laboratory under irrigation. Cereal rye has no significant effect on total soil water but, a significant reduction in cumulative subsurface drainage of 44% was determined at both sites. This study has shown the possible effect of cover crop on corn crop yield and soil properties over different regions in Nebraska. Future research extending the scope and geographic area is needed to test and quantify possible impacts of multiple CC species under diverse management and biophysical conditions.

Lymphatic Embolization versus Sclerotherapy for Symptomatic Post-operative Pelvic Lymphocele (LESPOL): A Randomized Pilot Study

This study was performed to compare clinical outcomes of lymphatic n-butyl cyanoacrylate (NBCA) embolization and ethanol sclerotherapy in patients with symptomatic postoperative pelvic lymphoceles. This prospective, open-label, randomized, and controlled trial took place at two medical centers in Korea. Patients ≥ 18 years old with symptomatic postoperative pelvic lymphoceles were randomly assigned (1:1) to one of two study arms: embolization or sclerotherapy. A target of 44 patients was set for each group to assess clinical outcomes. The trial began in August 2018 but ended as a pilot study in February 2020. Problematic patient enrollment precluded full population recruitment, only 11 patients (embolization, 5; sclerotherapy, 6) eventually participating. Clinical success and drainage tube removal rates were 100% in both arms. To achieve clinical success, significantly more procedures were needed in the sclerotherapy group than in the embolization group (4.5 ± 1.23 vs 2.0 ± 1.00; p < 0.001). The time to achieve daily drainage reduction thresholds (< 20 mL or < 20% of initial daily drainage) was also less in the sclerotherapy (vs embolization) group but fell short of statistical significance (4.8 ± 2.63 days vs 7.0 ± 3.67 days; p = 0.267). No significant adverse events or recurring symptomatic lymphoceles were evident at 3-month follow-up visits. Both NBCA embolization and ethanol sclerotherapy might be safe and effective in treating symptomatic postoperative pelvic lymphoceles, showing no significant difference in clinical outcomes. In a future randomized prospective study, it is essential to establish better inclusion criteria to selectively include intractable lymphoceles to find out the difference in the outcome between the two treatments. Trial registration: The study is registered as KCT0003078 at the Clinical Research Information Service ( http://cris.nih.go.kr ). Registered April 2, 2018.

Endoscopic ultrasound-guided intervention for inaccessible papilla in advanced malignant hilar biliary obstruction.

Advanced malignant hilar biliary obstruction (MHBO) with inaccessible papilla poses a significant challenge to endoscopists, as drainage of multiple liver segments may be warranted. Transpapillary drainage may not be feasible in patients with surgically altered anatomy, duodenal stenosis, prior duodenal self-expanding metal stent, and after initial transpapillary drainage, but require re-intervention for draining separated liver segments. Endoscopic ultrasound-guided biliary drainage (EUS-BD) and percutaneous trans-hepatic biliary drainage are the feasible options in this scenario. The major advantages of EUS-BD over percutaneous trans-hepatic biliary drainage include a reduction in patient discomfort and internal drainage away from the tumor, thus reducing the possibility of tissue or tumor ingrowth. With innovations, EUS-BD is helpful not only for bilateral communicating MHBO but also for non-communicating systems with bridging hilar stents or isolated right intra-hepatic duct drainage by hepatico-duodenostomy. EUS-guided multi-stent drainage with specially designed cannulas and guidewires has become a reality. A combined approach with endoscopic retrograde cholangiopancreatography for re-intervention, interventional radiology, and intraductal tumor ablative therapies has been reported. Stent migration and bile leakage can be minimized with proper stent selection and technique, and stent blocks can be managed with EUS-guided interventions in a majority of cases. Future comparative studies are required to establish the role of EUS-guided interventions in MHBO as rescue or primary therapy.

Hypothermia impairs glymphatic drainage in traumatic brain injury as assessed by dynamic contrast-enhanced MRI with intrathecal contrast.

The impact of hypothermia on the impaired drainage function of the glymphatic system in traumatic brain injury (TBI) is not understood. Male Sprague-Dawley rats undergoing controlled cortical impact injury (CCI) were subjected to hypothermia or normothermia treatment. The rats undergoing sham surgery without CCI were used as the control. Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) with intrathecal administration of low- and high-molecular-weight contrast agents (Gd-DTPA and hyaluronic acid conjugated Gd-DTPA) was performed after TBI and head temperature management. The semiquantitative kinetic parameters characterizing the contrast infusion and cleanout in the brain, including influx rate, efflux rate, and clearance duration, were calculated from the average time-intensity curves. The qualitative and semiquantitative results of DCE-MRI obtained from all examined perivascular spaces and most brain tissue regions showed a significantly increased influx rate and efflux rate and decreased clearance duration among all TBI animals, demonstrating a significant impairment of glymphatic drainage function. This glymphatic drainage dysfunction was exacerbated when additional hypothermia was applied. The early glymphatic drainage reduction induced by TBI and aggravated by hypothermia was linearly related to the late increased deposition of p-tau and beta-amyloid revealed by histopathologic and biochemical analysis and cognitive impairment assessed by the Barnes maze and novel object recognition test. The glymphatic system dysfunction induced by hypothermia may be an indirect alternative pathophysiological factor indicating injury to the brain after TBI. Longitudinal studies and targeted glymphatic dysfunction management are recommended to explore the potential effect of hypothermia in TBI.

Interplay of Interfacial and Rheological Properties on Drainage Reduction in CO2 Foam Stabilised by Surfactant/Nanoparticle Mixtures in Brine

Although nanoparticles (NPs) are known to increase foam stability, foam stabilisation is not observed in all surfactant/NP combinations. The present study evaluates the stability of CO2 foams containing surfactant/NP mixtures with attractive or repulsive electrostatic interactions at the low pH imposed by CO2 in the presence of a high-salinity brine. Three ionic surfactants and two oxide NPs (SiO2 and Al2O3) were used in combinations of similar or opposite charges. Surface tension, viscosity, ζ-potential and hydrodynamic size experiments allowed the analysis of CO2 foam stability based on the impact of surfactant–NP interactions on bulk and interfacial properties. All oppositely charged systems improved the foam half-life; however, a higher NP concentration was required to observe a significant effect when more efficient surfactants were present. Both bulk viscosity and rigidity of the interfacial films drastically increased in these systems, reducing foam drainage. The mixture of SiO2 with a zwitterionic surfactant showed the greatest increase in CO2 foam stability owing to the synergy of these effects, mediated by attractive interactions. This study showed that the use of NPs should be tailored to the surfactant of choice to achieve an interplay of interfacial and rheological properties able to reduce foam drainage in applications involving CO2 foam in brine.

Letter to the editor regarding the article by Elhage et al.: "Closed-incision negative pressure therapy at -125 mmHg significantly reduces surgical site complications following total hip and knee arthroplasties: A stratified meta-analysis of randomized controlled trials".

Letter to the editor regarding the article by Elhage et al.: "Closed-incision negative pressure therapy at -125 mmHg significantly reduces surgical site complications following total hip and knee arthroplasties: A stratified meta-analysis of randomized controlled trials".

Glycerol amendment enhances biosulfidogenesis in acid mine drainage-affected areas: An incubation column experiment.

Microbial sulfate (SO4 2−) reduction in Acid Mine Drainage (AMD) environments can ameliorate the acidity and extreme metal concentrations by consumption of protons via the reduction of SO4 2− to hydrogen sulfide (H2S) and the concomitant precipitation of metals as metal sulfides. The activity of sulfate-reducing bacteria can be stimulated by the amendment of suitable organic carbon sources in these generally oligotrophic environments. Here, we used incubation columns (IC) as model systems to investigate the effect of glycerol amendment on the microbial community composition and its effect on the geochemistry of sediment and waters in AMD environments. The ICs were built with natural water and sediments from four distinct AMD-affected sites with different nutrient regimes: the oligotrophic Filón Centro and Guadiana acidic pit lakes, the Tintillo river (Huelva, Spain) and the eutrophic Brunita pit lake (Murcia, Spain). Physicochemical parameters were monitored during 18 months, and the microbial community composition was determined at the end of incubation through 16S rRNA gene amplicon sequencing. SEM-EDX analysis of sediments and suspended particulate matter was performed to investigate the microbially-induced mineral (neo)formation. Glycerol amendment strongly triggered biosulfidogenesis in all ICs, with pH increase and metal sulfide formation, but the effect was much more pronounced in the ICs from oligotrophic systems. Analysis of the microbial community composition at the end of the incubations showed that the SRB Desulfosporosinus was among the dominant taxa observed in all sulfidogenic columns, whereas the SRB Desulfurispora, Desulfovibrio and Acididesulfobacillus appeared to be more site-specific. Formation of Fe3+ and Al3+ (oxy)hydroxysulfates was observed during the initial phase of incubation together with increasing pH while formation of metal sulfides (predominantly, Zn, Fe and Cu sulfides) was observed after 1–5 months of incubation. Chemical analysis of the aqueous phase at the end of incubation showed almost complete removal of dissolved metals (Cu, Zn, Cd) in the amended ICs, while Fe and SO4 2− increased towards the water-sediment interface, likely as a result of the reductive dissolution of Fe(III) minerals enhanced by Fe-reducing bacteria. The combined geochemical and microbiological analyses further establish the link between biosulfidogenesis and natural attenuation through metal sulfide formation and proton consumption.

Water flux and biomass production of native plants at different substrate compactions for landfill phytocaps in Southeast Queensland, Australia

Phytocaps for landfills and degraded sites have multiple benefits provided by the established vegetation, including reducing substrate moisture content and drainage through evapotranspiration. One of the factors affecting plant development measured by biomass production is substrate compaction. However, the relationship between drainage and biomass production related to substrate compaction is largely unknown. In an outdoor pot experiment, four native plant species: Eucalyptus terreticornis, Acacia concurrens, Allocasuarina littoralis, and Themeda triandra, grown in coal overburden at three different relative compactions (RC) levels (64%, 77% and 87% of the maximum compaction) in Southeast Queensland, Australia, over seven months. Evapotranspiration rate was measured by change in pot weight. Drainage for each pot was collected in a container after every irrigation and rainfall. Biomass of plants were harvested for dry shoot and root weight measurement. Additionally, root length density was obtained by a root scanner. Our statistical model predicted that 69.8% RC for maximum weekly ET. Overall, 77% RC treatment resulted in higher total evapotranspiration (1.5%), more significant shoot and root biomass production (17.2% and 11.8%, respectively), higher root length density (33.4%) and lower total drainage (20%) compared to 64% and 87% RC treatments. Native woody species had a higher rate of evapotranspiration and a lower rate of drainage than the native grass. Reduction in drainage was correlated with increase in evapotranspiration rate for all plant species, as the soil moisture content decreases through evapotranspiration. These findings support the notion of maximising evapotranspiration to minimise drainage, a key objective for phytocap design. From biomass production, root biomass closely correlates to a reduction in drainage. Multiple lines of evidence from this study indicate that 77% RC treatment maximises evapotranspiration and plant development and minimises deep drainage for phytocap applications, even though harsh microclimate in this experiment may have constrained plant development and affected the water flux.

Cellular Distribution of Brain Aquaporins and Their Contribution to Cerebrospinal Fluid Homeostasis and Hydrocephalus.

Brain aquaporins facilitate the movement of water between the four water compartments: blood, cerebrospinal fluid, interstitial fluid, and intracellular fluid. This work analyzes the expression of the four most abundant aquaporins (AQPs) (AQP1, AQP4, AQP9, and AQP11) in the brains of mice and discuss their contribution to hydrocephalus. We analyzed available data from single-cell RNA sequencing of the central nervous system of mice to describe the expression of aquaporins and compare their distribution with that based on qPCR, western blot, and immunohistochemistry assays. Expression of AQP1 in the apical cell membrane of choroid plexus epithelial cells and of AQP4 in ependymal cells, glia limitans, and astrocyte processes in the pericapillary end foot is consistent with the involvement of both proteins in cerebrospinal fluid homeostasis. The expression of both aquaporins compensates for experimentally induced hydrocephalus in the animals. Recent data demonstrate that hypoxia in aged animals alters AQP4 expression in the choroidal plexus and cortex, increasing the ventricle size and intraventricular pressure. Cerebral distensibility is reduced in parallel with a reduction in cerebrospinal fluid drainage and cognitive deterioration. We propose that aged mice chronically exposed to hypoxia represent an excellent experimental model for studying the pathophysiological characteristics of idiopathic normal pressure hydrocephalus and roles for AQPs in such disease.

Alternatives how to mitigate urban flooding and CSO in urbanized areas used in European cities and towns

Urban flooding and combined sewer overflow in city, or town areas represents potential risk in environmental, economic, or social aspects. The goal of this study is to process and evaluate efficiency of individual solutions to reduce occurrence of flooding in urban areas caused by intense rainfall events. The known conventional solutions are stormwater chambers, or storage drains. The new trend in reduction of stormwater drainage into combined sewer system are parts of blue-green infrastructure. Blue-green infrastructure represents environmental urban infrastructure which consists of sensitively selected urban vegetation combined with ingenious hydrological elements of urban city drainage. The study also deals with experimental usage of decentralized real time control, based on a gossip-based algorithm of moveable gates in sewage network. Experiment was proposed for drainage system of the city of Cosenza in Italy. Evaluation will assess application value of individual proposed solutions for the reduction of combined sewer overflow for Slovak republic and its urban cities, or towns.