DPPH Antioxidant Activity Research Articles

Optimization and evaluation of Atrina pectinata polysaccharides recovered by subcritical water extraction: A promising path to natural products

In this study, the recovery of Atrina pectinata posterior adductor polysaccharides (APP-PS) using subcritical water extraction (SWE) was optimized by response surface methodology (RSM) and the physicochemical and biological properties of the recovered APP-PS were evaluated. The optimal extraction conditions, which resulted in a maximum yield of 55.58 ± 1.12 %, were temperature, 152.08 °C; extraction time, 10 min; solid–liquid ratio, 30 g/600 mL. The obtained APP-PS was found to be 88.05 ± 0.17 % total sugar. Fourier transform infrared (FT-IR) and Nuclear magnetic resonance (NMR) analyses confirmed the presence of the α-coordination of D-glucan in the polymer sample. The analysis of monosaccharide composition, along with thermogravimetric analysis, revealed the typical structure of the sample, composed of glucose alone. Total phenolic contents of APP-PS were measured as 5.47 ± 0.01 mg Gallic acid/g of dry sample and total flavonoids contents were determined to be 0.78 ± 0.06 mg Quercetin/g of dry sample. For biological activities, ABTS+, DPPH and FRAP antioxidant activities were measured to be 20.00 ± 0.71, 2.35 ± 0.05 and 4.02 ± 0.07 μg Trolox equivalent/100 g of dry sample, respectively. Additionally ACE inhibitory was confirmed to be 87.02 ± 0.47 %. These results showed that SWE is an effective method to recover biofunctional materials from marine organisms.

Synthesis, in-vitro antioxidant and anti-inflammatory properties of novel amide derivatives of substituted 2-aminothiophenes and 3,4-dimethoxy cinnamic acid

Novel amide derivatives using substituted 2-aminothiophenes (1A–12A) were synthesized by following a suitable procedure. The synthesized compounds were characterized by physical and spectral data. The compounds were screened for in-vitro antioxidant and anti-inflammatory activities with reference to the standard drugs, Ascorbic acid and Ibuprofen respectively. The majority of the compounds showed good antioxidant activity in DPPH and hydroxyl radical scavenging assays. The synthesized compounds were also tested for in-vitro anti-inflammatory activity by protein denaturation and HRBC membrane stabilization methods. Among all, the compounds 2A and 3A showed significant in-vitro anti-inflammatory activity when compared with the standard drug Ibuprofen.

Increased expression of GLUT4, catalase and nitric oxide by a crystalised fraction from ethanolic extracts of oak leaf lettuce and okra seed in C2C12 cell line

The fractions obtained from low temperature-induced crystallisation of ethanolic extracts of green- and red-oak leaf and okra seed were accounted for total polyphenol content and the DPPH antioxidant activity, resulting in 5.99, 4.44, and 12.35 mg gallic acid equivalent per g sample, and 0.45, 0.35, and 0.99 mg Trolox equivalent per g sample, respectively. Insulin resistance was the result after incubating C2C12 skeletal muscle cells in high glucose DMEM for 20 h. Oxidative stress and inflammation were triggered by re-incubating these resistant cells with insulin. The expression of p-p38 MAPK and NF-B proteins and the NF-B p65 transcription factor activity were enhanced. Such inflammatory protein markers were reduced when the crystalised extracts replaced insulin. Increased catalase activity and NO production were also determined for the incubations using the crystalised extracts. It was suitable to include these vegetables in the daily diets of prediabetic individuals for better management of the patient’s health status by increasing insulin sensitivity and decreasing inflammation.

Combining Natural Ingredients to Optimise Antioxidant Content in Brewing Herbal Drinks

Herbal drinks are one of the functional foods utilized and needed by the community to maintain health. Herbal drinks consisting of green tea, noni leaves, and butterfly pea flowers can be used to meet the community’s needs. The research was conducted by optimizing the combination of natural ingredients to obtain high antioxidant content in herbal drinks. Optimization using RSM Method on Minitab 17, with 2 factors of green tea concentration and maximum concentration of noni leaves 2.4 g, using Central Composite Design with a combination of 13 treatments, one replication. The main parameter tested was DPPH antioxidant activity as a response to the treatments tried. The results obtained were that there was an interaction between the components of green tea and noni leaves on the antioxidant activity of counteracting DPPH free radicals, with the equation: DPPH = 89.8 + 4.2 GT - 23 NL + 5.6 GT * GT + 14.2 NL * NL - 0.58 GT * NL. it can be concluded that there is an interaction between the two ingredients used in the antioxidant activity of the herbal drink.

Antioxidant study and Molecular docking of sugar-triazole derivatives

We synthesized a several of sugars-triazole derivatives containing different groups and studied their biological activity with studding molecular docking of these derivatives. To determination antioxidant activity of these derivatives DPPH was used for this purpose. DPPH radicals react with reactive species and scavenging ROS when antioxidant reacted with DPPH radical when donate a hydrogen atom or transfer electron. Molecular docking data of derivatives with spike covid19 (6W41) gives good results can be used in future on how to deal with and treat the virus.

Determination of Antioxidant Activities in Raw and Boiled Extractions of Raphanus raphanistrum L. Species Naturally Growing in Edremit Gulf (Balıkesir/Turkey)

The plants that naturally spread in Edremit Gulf are consumed by the local people in various ways either cooked or raw. In this study, Raphanus raphanistrum species from the Brassicaceae family, which is also known to be used as food, was used. DPPH and CUPRAC antioxidant activity analyses were carried out on the extracts obtained from the aerial parts of the raw and boiled fresh plant. The total phenolic contents were determined and the phenolic compounds contained and their interactions with the boiling process were determined by HPLC method. The extracts were stored in a deep freezer at -20 °C to be used in further studies. In terms of the total amount of phenolic compounds, 12.65 µg GAE/mg extract was detected in the boiled extract of R. raphanistrum species, and it has a higher value compared to the raw extract. In the boiled extract, which has a higher value in terms of total flavonoid content than raw, was found 54 µg Rutin Equivalent /mg fresh extract. Compared to the CUPRAC method, the boiled extract showed higher (68.58 µgTE/mg extract) activity. The antioxidant activity of the boiled extract was found to be higher than the DPPH method, and the EC 50 value was measured as 1975.25 µg/mL. According to HPLC method, resveratrol, rosmarinic acid, quersetin, kaempferol and naringenin substances were determined as in the raw extract. It was determined that while naringenin was destroyed in the boiled extract, were changed the amounts of other phenolic compounds.

Properties of an Emulsion Gel with Gracilaria fisheri Agar, Whey Protein Isolate and Black Seed Oil

Gracilaria fisheri agar has beneficial potential for human health; however, its application in food products needs to be incorporated into an emulsion gel due to its low gel strength. A response surface methodology with a central composite design was utilized to prepare emulsion gels containing 4–6% Gracilaria fisheri Agar (AGF), 4–8% Whey Protein Isolate (WPI) and 10–20% Black Seed Oil (BSO). The optimum levels of AGF, WPI, and BSO were determined to be 6%, 4% and 10% respectively to give the preferred gel strength of 966.24 g, water holding capacity of 96.12%, and DPPH antioxidant activity of 59.16%.

Comparative analysis of total flavonoid, total phenolic content, and antioxidant activity of Theobroma cacao Pod Husk extract and its derived fractions

Secondary metabolites from plants are known to be promising sources of raw materials for drugs in terms of safety. This research focuses on cacao pod husk as a candidate for discovering natural antioxidant compounds. The stages in this study include the extraction of cacao pod husk using methanol as a solvent, followed by liquid-liquid fractionation to produce ethyl acetate and acetone fractions. Subsequently, total flavonoid and phenolic content, DPPH antioxidant activity tests, and GCMS metabolite analysis were conducted. The research results indicate that the ethyl acetate fraction has the highest total flavonoid and phenolic content compared to methanol extract and acetone fraction. The methanol extract has the highest antioxidant activity with an IC50 of 0.0736 mg/mL. The identified metabolites belong to the groups of polyphenols, flavonoids, steroids, and their derivatives, as well as some volatile compounds such as hexadecanoic acid methyl ester. Compounds with potential antioxidant properties include Eugenol, Myristicine, Piperine, and Theobromine.

Preparation, stability and digestive properties of tea polysaccharide nanoparticles

This study aimed to investigate the storage stability and functional properties of different sizes of tea polysaccharides, and explore the relationship between sizes and digestive properties, so as to provide reference for the subsequent utilization of tea polysaccharides. In this study, TPS-NPs of about 200 nm and 400 nm were prepared by electrostatic interaction with TPS and EDTA ratios of 6:1 and 100:1, respectively. The structural properties of TPS-NPs were investigated by a series of characterization analyses. The results showed that the major functional groups of TPS-NPs did not change significantly with particle size, but the CO stretching vibrations in the methylated carboxyl group were more pronounced. However, their DPPH free radical scavenging ability and antioxidant activity of TPS were decreased in vitro because of the destruction of the apparent structure of tea polysaccharides nanoparticles during preparation. Stability measurements revealed that TPS-NPs have high storage, temperature, pH and ionic concentration stability, especially for TPS-NPs with a particle size of 200 nm. The digestibility of 400 nm TPS-NPs was improved to 3.89% compared to TPS. The results showed that the TPS-NPs has excellent stability and digestibility, which contributed to the development of TPS applications in the food industry and related products.

Comparative evaluation of phytochemical, in vitro antioxidant activities and elemental composition of the fruit, leaves, and stem bark of Tetrapleura tetraptera

Introduction: Nowadays, medicinal plants are widely accepted as a better option than synthetic medications due to their lower cost and little to no side effects. The current study aimed to compare the elemental composition, phytochemical profile, and antioxidant efficacy of n-hexane extracts derived from Tetrapleura tetraptera fruit, stem bark, and leaves. Methods: The fresh fruits, leaves, and stem bark of the plant were collected and dried. The dried samples were pulverized and exhaustively extracted with n-hexane. The extracts were screened for both qualitative and quantitative phytochemical constituents. Antioxidant parameters such as Ferric Reducing Antioxidant Property (FRAP) and 2,2-Diphenyl-1-picryl hydroxyl radical scavenging activity (DPPH) were also carried out. The elemental analysis of the various organs was also evaluated using standard protocols. Results: The presence of alkaloids, flavonoids, tannins, saponins, terpenes, resins, and phenolswas revealed in the phytochemical data. The stem bark of T. tetraptera has higher concentrations of alkaloids and flavonoids than the leaf and fruit, according to a quantitative examination of the phytochemical elements of the plant. The stem bark had the highest Total Phenolic Content at 1000 µg/mL, whereas the leaves had the lowest TPC at 200 µg/mL.At a concentration of 1000 µg/mL, the stem bark exhibited the greatest activity of total flavonoid (TFC) in the plant extracts, followed by the fruits and leaves. At 1000 µg/mL concentration, the FRAP test revealed the maximum activity in the stem bark. Generally speaking, when concentration rises, so do the TPC, TFC, and FRAP activities. Compared to the standard ascorbic acid (2.76 µg/mL), stem bark exhibited the highest level of DPPH antioxidant activity, with an IC 50 of 13.34 µg/mL. The elemental analysis revealed that the stem bark had the highest concentrations of Ca, Mg, and Fe, followed by the fruit and leaves, in that order.Conclusion: This research result has established that the plant, T tetraptera can be standardized and offer an alternative eco-friendly drug for immunity building. The stem barks are underutilized, however, its phytochemical studies and other investigations pointed to its potential in the cosmeceutical industries and medicine. Keywords: Antioxidant activity, ethnomedicine, ferric-reducing assay, medicinal plant

Assessment of physicochemical parameters, and antioxidant properties of mango concentrate during different storage intervals

ABSTRACT Pakistan is one of the biggest mango producing country in the world. Post-harvest losses of mango occur due to lack of storage facilities. Mango can be converted into concentrate to utilize it for longer period. In the current study, the physicochemical profile and storage stability of mango concentrate was investigated to evidence the opportunity of preservation method. Three different concentrations (0.1%, 0.2%, and 0.5%) of citric acid, sodium benzoate and potassium sorbate were applied. All chemical preservatives significantly (p ˂ 0.05) increased viscosity, total soluble solids (TS), reducing sugar (RS), non-reducing sugar (NRS), total soluble solids (TSS), and titratable acidity (TA), while the moisture content, pH, DPPH antioxidant activity, TPC (total phenolic content), TFC (total flavonoid content) and the level of texture acceptance decreased during the storage. The moisture content was decreased from 43.4% to 34.9% during storage. Phytochemical analysis showed that mango concentrate is a potential source of antioxidant compounds and has higher DPPH radical scavenging activity. Regarding preservation solution, it is suggested that sodium benzoate and citric acid might be utilized for industrial applications.

Psephellus huber-morathii (Wagenitz) Wagenitz'in potansiyelini keşfetme: Fitokimyasal bileşiminin kapsamlı bir UHPLC-MS/MS analizi ve antioksidan, antimikrobiyal ve antiproliferatif aktivitelerinin değerlendirmesi

Aims: Psephellus huber-morathii (PH) is an endemic species in the Eastern Anatolia Region. In this study, besides the biological activities of the 70% methanol extract of PH, its comprehensive phytochemical composition was investigated.
 Material and methods: The phenolic composition of the PH extract was analyzed using the UHPLC-MS/MS method. To evaluate its antimicrobial characteristics, the microdilution technique was employed. The antioxidant capabilities, total phenolic and flavonoid contents of the extract were determined using the spectrophotometer. Additionally, the effects of the extracts on cell proliferation and NCI-60 survival parameter values were assessed using the MTT assay.
 Results: Quinic acid and chlorogenic acid were major compounds. The extract showed high antioxidant activity in DPPH (IC50:13.9±0.4 μg mL-1) and FRAP (61.3±2.3 mg TE g extract-1) methods. The total phenolic and flavonoid contents of the extract were found as 52.2±1.9 mg GAE g extract-1 and 28.6±0.9 mg QE g extract-1, respectively. 
 Discussion: The extract showed low antiproliferative activity against normal lung and retinal cell lines, promising anticancer effect on gynecological and colon cancer cells, and moderate antimicrobial activity against Bacillus cereus and Enterococcus faecium.
 Conclusions: The study demonstrated the medicinal potential and value of PH extract against infectious diseases and cancer.

Anti-inflammatory activity of verbascoside- and isoverbascoside-rich Lamiales medicinal plants

Verbascoside and isoverbascoside are two active phenylethanoid glycosides mainly found in plants of the order Lamiales. This study analyzes the verbascoside and isoverbascoside levels and the total phenolic contents in the water and ethanolic extracts of 20 medicinal plants of the order Lamiales commonly used in Thailand. The related bioactivities, including the antioxidant activity via the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reduction activity potential assays and anti-tyrosinase and -inflammatory activities via the cyclooxygenase and nitric oxide assays are also investigated. The extracts of several plant species, including Barleria prionitis, B. lupulina, Rhinacanthus nasutus, Orthosiphon aristatus, and Nicoteba betonica, exhibit high verbascoside and isoverbascoside content levels. The correlation analysis between the bioactive activities and the active compounds demonstrates a significant association between the verbascoside level in the water extracts and both the DPPH antioxidant activity and the nitric oxide level in the anti-inflammatory assays. This study provides the first report on the verbascoside and isoverbascoside quantification of several plant samples. The findings provide valuable insights for future research on lesser-studied plants possessing high verbascoside and isoverbascoside levels, which exhibit promising anti-inflammatory activities.

Impact of In Vitro Digestion on the Digestibility, Amino Acid Release, and Antioxidant Activity of Amaranth (Amaranthus cruentus L.) and Cañihua (Chenopodium pallidicaule Aellen) Proteins in Caco-2 and HepG2 Cells.

This study evaluated the impact of in vitro gastrointestinal digestion on the digestibility, amino acid release, and antioxidant activity of proteins from amaranth (Amarantus cruentus L.) and cañihua (Chenopodium pallidicaule Aellen). Antioxidant activity was assessed using ORAC, ABTS, DPPH, and cellular antioxidant activity (CAA) assays in human intestinal Caco-2 and hepatic Hep-G2 cell lines. The results showed that amaranth had higher protein digestibility (79.19%) than cañihua (71.22%). In addition, intestinal digestion promoted the release of essential amino acids, such as leucine, lysine, and phenylalanine, in both protein concentrates. Concentrations of amaranth and cañihua proteins, ranging from 0.125 to 1.0 mg mL-1, were non-cytotoxic in both cell lines. At a concentration of 0.750 mg mL-1, simulated gastrointestinal digestion enhanced cellular antioxidant activity. Intestinal digest fractions containing peptides >5 kDa were the principal contributors to CAA in both cell lines. Notably, cañihua proteins exhibited high CAA, reaching values of 85.55% and 82.57% in Caco-2 and HepG2 cells, respectively, compared to amaranth proteins, which reached 84.68% in Caco-2 and 81.06% in HepG2 cells. In conclusion, both amaranth and cañihua proteins, after simulated gastrointestinal digestion, showcased high digestibility and released peptides and amino acids with potent antioxidant properties, underscoring their potential health benefits.

Pengembangan produk teh kulit buah kopi dari tiga klon kopi robusta

Coffee skin is a waste from green coffee production as much as 40-45% which has the potential as a source of natural antioxidants. The diversity of local Robusta coffee clones in West Lampung as raw materials for the processing of coffee products provides a variety of coffee skins which are thought to have strong antioxidant activity which is useful as a functional drink for coffee skin tea (cascara).The aims of the study werestudy aimed of the study were to identify the sweetness level of coffee cherries skin from three local coffee robusta clones (rope, huerona, and srintil), identify antioxidant activity, test consumer preferences, and calculate the added value of coffee skin tea (cascara). The research method uses DPPH antioxidant activity analysis, consumer preference test (organoleptic hedonic test), and the calculation of added valueadded value calculation using the Hayami method. The results showed that the antioxidant activity of the Rope clone had strong antioxidant activity, the cascara drink on the level of aroma, taste, and liking for cascara tea found no difference between samples based on analysis of variance, but. Still, the cascara drink from the Rope clone had a darker color. The added value generated by the cascara is IDR 27,941 per kilogram of raw materials used

Evaluation of several macroalgae species on methane emission and antioxidant activity based on in vitro rumen fermentation characteristics

Marine macroalgae are the prospective and promising Indonesian natural resources containing bioactive compounds. This study aimed at evaluating various seaweed species with optimal biological activity to mitigate in vitro rumen methane emission and to confirm their antioxidant properties. In addition, relationships among several phenolic fractions and methane (CH4) production of seaweeds were elucidated by Pearson Correlation to screen seaweeds for their capacity to reduce CH4 formation. For in vitro batch cultures experiment, the diets were 500 mg of a substrate of seaweed. They were incubated with 50 ml of buffered rumen fluid (1:2 ratio of rumen fluid to buffer media) in a 100 ml serum vial at 39°C for 72 hours incubation time. A randomized block design was carried out with 14 dried seaweed treatments and 5 different cattle rumen fluid groups as a block. For total phenol, flavonoid, and antioxidant activity, a completely randomized design with 14 treatments and 3 replications was carried out. Data were analyzed by Analysis of Variance (ANOVA) and Duncan analysis. The results showed that the lowest methane production (ml/g DM incubated) significantly (P<0.05) was reduced by Halymenia sp. (0.24) and Caulerpa racemosa (0.41). Caulerpa racemosa showed high flavonoid content (1.5 mg Quercetin/g) and Ulva lactuca had the highest phenol content (0.55 mg Gallic Acid/g). Gracilaria coronopifolia had strong antioxidant activity DPPH radical scavenging activity (77.5% inhibition). It was concluded that Halymenia sp., Caulerpa racemosa and Gracilaria coronopifolia could be used as methane inhibitors and antioxidants for ruminants.

Synthesis and biological evaluation of ciprofloxacin – 1,2,3-triazole hybrids as antitumor, antibacterial, and antioxidant agents

Six novel ciprofloxacin-1,2,3-triazole hybrids (6a-f) were synthesized via click reaction, by reacting of methyl 1-cyclopropyl-6-fluoro-4-oxo-7-(4-(3-oxobutanoyl)piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylate (5) with various aryl azides (9a-f). The new compounds were characterized using High-Resolution Mass Spectrometry (HRMS), 1H NMR, 13C NMR, and elemental analysis. Compounds (6a-f) screened for their in vitro anticancer activity against three cell lines, namely, non-small cell lung cancer (A549), glioblastoma (U-87 MG), and breast cancer (MCF7). Hybrids 6a and 6b exhibited remarkable anti-proliferative activity against all three cell-lines. IC50 values of 6b for all cancer cell lines were significantly lower comparing to the standard reference compound IC50. The IC50 of 6b for the normal cell (HDF) line was significantly higher than the reported for cisplatin [IC50 = 170.7 ± 8.1 μM/ml (HDF), (p ≤ 0.001)], indicating less toxicity towards normal cells and thereby has a better therapeutic index, with a selectivity index of 142.3 for U87 cell line. Compounds 6e, 6d, and 6f displayed significant cytotoxic activity against only U-87 and MCF-7 cancer cell lines, compared to normal cells (HDF). Compound 6f [IC50 = 7.9 ± 2.3 μM/ml (U-87) and 10.6 ± 3 μM/ml (MCF-7)] was more potent than cisplatin [IC50 = 28.3 ± 5.3 μM/ml (U-87) and 26.9 ± 4.7 μM/ml (MCF-7)] in displaying anti-proliferative effect against U-87 and MCF-7 cells, with less cytotoxic to normal cells [IC50 = 141.7 ± 4.1] than cisplatin [IC50 = 40.9 ± 5.4]. Moreover, they were tested for their antioxidant activity in DPPH and ABTS assays and antibacterial activity.

Endemic Mountain Tea (Sideritis dichotoma Huter): Antioxidant and Antibacterial Activities, Mineral Content by ICP-MS and Phytochemical Content by LCHR-MS.

Endemic Sideritis dichotoma Huter, herbal tea, is used for colds, coughs and digestive ailments. Since at least 25 % of the active ingredients of pharmacological drugs are obtained from plants, it is important to investigate many plants, both traditionally used and whose potential hasnt yet been discovered, before they disappear. When the results compared to other Sideritis species, it has better antioxidant activity in DPPH⋅ scavenging activity, Fe3+ and Cu2+ reducing capacity methods and is also rich in minerals, necessary for human body, K, Mg, Ca, P, S. Zone values (10-19,5 mm) obtained on S. aureus, P. aeruginosa, E. coli, S.enteritidis, E. faecalis, P. putida and K. pnemoniae strains are an indication that the plant has antibacterial activity. 27 secondary metabolites were identified by LC-HRMS. Verbascoside, pharmacologically active compound known to have antitumor effect, cytotoxic selectivity and anticancer activity, is the most abundant phenolic in ethanol extract (49297.13 mg/kg).

Unveiling the Impact of Drying Methods on Phytochemical Composition and Antioxidant Activity of Anthemis palestina.

Different drying techniques may alter the chemical composition of plant extracts and consequently affect their bioactivity potential. The current study was designed to reveal the effect of four different drying methods on the phytochemical composition and antioxidant activity of hydrodistilled essential oil (HD-EO) and methanolic (APM) extract obtained from the aerial part of Anthemis palestina from Jordan. Aerial parts of A. palestina in their fresh (FR) form and after drying in shade (ShD), sun (SD), oven at 40 °C (O40D) and 60 °C (O60D), in addition to microwave (MWD), were used to extract their essential oils by hydrodistillation and to prepare the different methanolic extracts (APM). GC/MS analysis of the different HD-EOs revealed qualitative and quantitative differences among the different samples. While FR, O40D, O60D, and MWD EO samples contained mainly sesquiterpene hydrocarbons (35.43%, 29.04%, 53.69%, and 59.38%, respectively), ShD sample was rich in oxygenated monoterpenes (33.57%), and SD-EO contained mainly oxygenated sesquiterpenes (40.36%). Principal component analysis (PCA) and Cluster analysis (CA) grouped the different drying methods based on their impact on the concentration of chemical constituents. SD-EO demonstrated high DPPH and ABTS antioxidant activity (1.31 ± 0.03) × 10-2; (1.66 ± 0.06) × 10-2 μg/mL, respectively). Furthermore, A. paleistina methanolic extracts (APM) obtained after subjecting the plant to different drying methods showed interesting patterns in terms of their TPC, TFC, antioxidant activity, and phytochemical profiling. Of all extracts, SD-APM extract had the highest TPC (105.37 ± 0.19 mg GA/g DE), highest TFC (305.16 ± 3.93 mg Q/g DE) and demonstrated the highest DPPH and ABTS scavenging activities ((4.42 ± 0.02) × 10-2; (3.87 ± 0.02) × 10-2 mg/mL, respectively); all were supported by correlation studies. LC-MS/MS analysis of the different extracts revealed the richness of the SD-APM extract in phenolic acids and flavonoids.

The ethanol extract of Syzygium cumini exhibits cytotoxic potentials against breast cancer cells due to antioxidant properties

The present investigation was designed to evaluate in vitro cytotoxic potentials of ethanol extract of Syzygium cumini against breast cancer cells and also estimate its antioxidant activity. The ethanol extract of Syzygium cumini was prepared by successive solvent extraction process using sox-let apparatus. The ethanol extract was evaluated for its in-vitro anticancer potentials against MCF-7 human breast cancer cell lines by MTT assay and concentration of the ethanol extract required to inhibit 50% of cell growth (IC50) was recorded. To determine antioxidant properties of the extract, superoxide scavenging, lipid peroxidation and DPPH methods were used and IC50 values of extracts were noted. In current study, ethanol extract of Syzygium cumini has shown anticancer property by exhibiting significant IC50 values against growth of breast cancer cell growth. The extract has also shown significant IC50 values in superoxide scavenging, lipid peroxidation and DPPH methods and antioxidant activity was comparable to standard drug ascorbic acid. The results obtained from the present research work suggested that the ethanol extract of Syzygium cumini possess significant in vitro cytotoxic potentials against breast cancer cells. The results also suggested that the ethanol extract have in vitro antioxidant properties.&#x0D; Keywords: Anticancer activity, Syzygium cumini, IC50 value, MTT assay and antioxidant activity.