Downstream Channel Research Articles

The role of CBL-CIPK signaling in plant responses to biotic and abiotic stresses.

Plants have a variety of regulatory mechanisms to perceive, transduce, and respond to biotic and abiotic stress. One such mechanism is the calcium-sensing CBL-CIPK system responsible for the sensing of specific stressors, such as drought or pathogens. CBLs perceive and bind Calcium (Ca2+) in response to stress and then interact with CIPKs to form an activated complex. This leads to the phosphorylation of downstream targets, including transporters and ion channels, and modulates transcription factor levels and the consequent levels of stress-associated genes. This review describes the mechanisms underlying the response of the CBL-CIPK pathway to biotic and abiotic stresses, including regulating ion transport channels, coordinating plant hormone signal transduction, and pathways related to ROS signaling. Investigation of the function of the CBL-CIPK pathway is important for understanding plant stress tolerance and provides a promising avenue for molecular breeding.

A key gene, violaxanthin de-epoxidase-like 1, enhances fucoxanthin accumulation in Phaeodactylum tricornutum

BackgroundFucoxanthin has been widely investigated owing to its beneficial biological properties, and the model diatom Phaeodactylum tricornutum, possessing fucoxanthin (Fux) chlorophyll proteins as light-harvesting systems, is considered to have the potential to become a commercial cell factory for the pigment production.ResultsHere, we compared the pigment contents in 10 different P. tricornutum strains from the globe, and found that strain CCMP631 (Pt6) exhibited the highest Fux content but with a low biomass. Comparison of mRNA levels revealed that higher Fux content in Pt6 was related with the higher expression of gene violaxanthin de-epoxidase-like (VDL) protein 1 (VDL1), which encodes the enzyme catalyzing the tautomerization of violaxanthin to neoxanthin in Fux biosynthesis pathway. Single nucleotide variants of VDL1 gene and allele-specific expression in strains Pt1 (the whole genome sequenced strain CCMP632) and Pt6 were analyzed, and overexpressing of each of the 4 VDL1 alleles, two from Pt1 and two from Pt6, in strain Pt1 leads to an increase in downstream product diadinoxanthin and channels the pigments towards Fux biosynthesis. All the 8 VDL1 overexpression (OE) lines showed significant increases by 8.2 to 41.7% in Fux content without compromising growth, and VDL1 Allele 2 OE lines even exhibited the higher cell density on day 8, with an increase by 24.2–28.7% in two Pt1VDL1-allele 2 OE lines and 7.1–11.1% in two Pt6VDL1-allele 2 OE lines, respectively.ConclusionsThe results reveal VDL1, localized in the plastid stroma, plays a key role in Fux over-accumulation in P. tricornutum. Overexpressing VDL1, especially allele 2, improved both the Fux content and growth rate, which provides a new strategy for the manipulation of Fux production in the future.

The impact of dams and sand-gravel extractions on the downstream channel deformations (in the example of the Goychay River)

The impact of dams and sand-gravel extractions on the downstream channel deformations (in the example of the Goychay River)

Analyzing river disruption factors and ecological flow in China's Liu River Basin amid environmental changes.

Water resources variability and availability in a basin affect river flows and sustain river ecosystems. Climate change and human activities disrupt runoff sequences, causing water environmental issues like river channel interruptions. Therefore, determining ecological flow in changing environments is challenging in hydrological research. Based on an analysis of long-term changes in hydrological and meteorological variables and interruption conditions in the semi-arid Liu River Basin (LRB), this study summarizes the controlling factors of river interruption at different temporal and spatial scales and proposes a framework to determine ecological flow under changing environments. Hydrological model and the monthly optimal probability distribution were used to determine the optimal ecological runoff of LRB. The results showed that from 1956 to 2017, precipitation and potential evapotranspiration in the basin showed no significant decreasing trend, but the streamflow significantly decreased, and the downstream interruption worsened, with an average annual interruption duration of 194days at Xinmin Station from 1988 to 2017. The controlling factors of river interruption are as follows: (1) soil and water conservation measures in the upstream significantly reduce the runoff capacity; (2) the operation mode of the controlling reservoir in the middle reaches changes from "all-year discharge" to "winter storage and spring release" to "combined storage and supply," severing the hydraulic connection between upstream and downstream; and (3) siltation in the downstream river channel coupled with over-extraction of groundwater increases the seepage capacity of the river. The monthly ecological flow of Naodehai Reservoir was determined by considering the monthly seepage losses after reconstructing the natural runoff using the SWAT model and determining the optimal probability distribution function for monthly runoff. The findings are important for downstream LRB ecological restoration and for determining the ecological flow of other river basins in changing environments.

Flood Modeling in a Composite System Consisting of River Channels, Flood Storage Areas, Floodplain Areas, Polder Areas, and Flood-Control-Protected Areas

The Linhuaigang flood control project (LFCP), situated on the Huaihe River, China, uses the river channels upstream of the LFCP, together with the hinterland areas outside the channels, to retain and store fluvial floodwaters that exceed the downstream channel’s discharge capacity. The hinterland areas are split into seven flood storage areas, three floodplain areas, eight polder areas, and three flood-control-protected areas, and they are connected to the river in various ways. A coupled hydrodynamic model was established to simulate the hydrodynamic and water volume exchange between the river channels and the hinterland areas. The flood storage area, under the control of a flood diversion sluice, was simulated with a 2D hydrodynamic model, and the inflow process initiated by the flood diversion sluice was simulated as a control structure. The polder area was generalized as a reservoir that would be filled in several hours once put into use because of its small size. The uncontrolled inflow process between the flood-control-protected areas and the channel was simulated by means of a dam break model, which could simulate levee breaching. The flooding within the flood-control-protected area, which represents a vast space, was simulated with a 2D hydrodynamic model. The floodplain area was laterally connected to the river channel along the river levee. The difference between the simulated and the measured flood peak water stage did not exceed 0.2 m in 2003 and 2007, indicating that the accuracy of the model was relatively high. In the scenario of a design flood with a return period of 100 years, the flood storage areas and the LFCP were used in the following order: Mengwa, Qiujiahu, Nanrunduan, Shouxihu, Jiangtanghu, Chengxihu, Chengdonghu, and the LFCP. When the Huaihe River encounters a flood with a return period of 1000 years that exceeds the design standard, the highest water stage upstream of the LFCP and Zhengyangguan shall not exceed 29.30 m and 27.96 m after the use of all the flood storage areas, floodplain areas, and flood-control-protected areas. The results of this research can provide technical support for the flood risk management of the LFCP.

Effects of baffle position in serpentine flow channel on the performance of proton exchange membrane fuel cells

This study used a three-dimensional numerical model of a proton exchange membrane fuel cell with five types of channels: a smooth channel (Case 1); eight rectangular baffles were arranged in the upstream (Case 2), midstream (Case 3), downstream (Case 4), and entire cathode flow channel (Case 5) to study the effects of baffle position on mass transport, power density, net power, etc. Moreover, the effects of back pressure and humidity on the voltage were investigated. Results showed that compared to smooth channels, the oxygen and water transport facilitation at the GDL-Channel interface were added 11.53%−20.60% and 7.81%−9.80% at 1.68 A·cm−2 by adding baffles. The closer the baffles were to upstream, the higher the total oxygen flux, but the lower the flux uniformity and the worse the water removal. The oxygen flux of upstream baffles was 8.14% higher than that of downstream baffles, but oxygen flux uniformity decreased by 18.96% at 1.68 A·cm−2. The order of water removal and voltage improvement was Case 4 > Case 5 > Case 3 > Case 2 > Case 1. Net power of Case 4 was 9.87% higher than smooth channel. To the Case 4, when the cell worked under low back pressure or high humidity, the voltage increments were higher. The potential increment for the backpressure of 0 atm was 0.9% higher than that of 2 atm. The potential increment for the humidity of 100% was 7.89% higher than that of 50%.

Modulation of Cerebrospinal Fluid Dysregulation via a SPAK and OSR1 Targeted Framework Nucleic Acid in Hydrocephalus.

Hydrocephalus is one of the most common brain disorders and a life-long incurable condition. An empirical "one-size-fits-all" approach of cerebrospinal fluid (CSF) shunting remains the mainstay of hydrocephalus treatment and effective pharmacotherapy options are currently lacking. Macrophage-mediated ChP inflammation and CSF hypersecretion have recently been identified as a significant discovery in the pathogenesis of hydrocephalus. In this study, a pioneering DNA nano-drug (TSOs) is developed by modifying S2 ssDNA and S4 ssDNA with SPAK ASO and OSR1 ASO in tetrahedral framework nucleic acids (tFNAs) and synthesis via a one-pot annealing procedure. This construct can significantly knockdown the expression of SPAK and OSR1, along with their downstream ion channel proteins in ChP epithelial cells, thereby leading to a decrease in CSF secretion. Moreover, these findings indicate that TSOs effectively inhibit the M0 to M1 phenotypic switch of ChP macrophages via the MAPK pathways, thus mitigating the cytokine storm. In in vivo post-hemorrhagic hydrocephalus (PHH) models, TSOs significantly reduce CSF secretion rates, alleviate ChP inflammation, and prevent the onset of hydrocephalus. These compelling results highlight the potential of TSOs as a promising therapeutic option for managing hydrocephalus, with significant applications in the future.

Enhancing flow boiling using a microchannel with pillar–cavity mixed structures: A lattice Boltzmann study

Enhancement of flow boiling in microchannels through adjusting surface structures has attracted much attention in recent years. However, most of the existing studies focus on homogeneous surface structures. In the present study, a novel vertical microchannel with pillar–cavity mixed structures is conceived to enhance flow boiling heat transfer. In the mixed microchannel, cavities and pillars are distributed on the vertical sidewalls of the upstream and downstream flow channel, respectively. A multicomponent phase-change lattice Boltzmann model is employed to investigate the flow boiling performance of the mixed microchannel. Numerical results show that the cavities in the mixed microchannel can supply effective nucleation sites for timely departure of bubbles, while the pillars in the mixed microchannel can suppress the expansion of the vapor film from the outlet toward the inlet. Moreover, the bubbles from the upstream cavities can entrain the cold liquid to disrupt the vapor film covering the downstream pillars for the rewetting of the heated surface. As a result, the flow boiling performance can be significantly enhanced by the synergistic effect of the pillar and cavity structures, and the best flow boiling performance can be achieved by controlling the ratio of the number of cavities to the total number of structures in the mixed microchannel to optimize the synergistic effect. The influences of the structural parameters of pillars and cavities on the flow boiling performance have also been studied. It is found that the height of the pillars and the depth of the cavities have important influences on the flow boiling performance, while the boiling performance is not sensitive to the width of the pillars.

A study on the classification of geological background source cadmium migration phases in Zhejiang Province, China

The release of cadmium during the natural weathering process of cadmium-containing strata is a significant source of cadmium pollution in both water and soil. The Hetang formations, wildly located in Jiangxi Province Zhejiang Province and Anhui Province in China, is a typical example of a cadmium-rich black shale stratum and is one of the primary sources of soil cadmium contamination. The bottom coal seam of this formation has been found to contain high levels of cadmium, with concentrations up to 11.26 mg/kg. As a result of water flow eroding the riverbed, parent rocks containing cadmium undergo continuous weathering, releasing cadmium elements. This process contributes to cadmium pollution in downstream soil, originating from the geological background. By utilizing techniques such as FE-SEM, EDS, and optical microscopy, this study investigates the distribution of iron in rock fragments with varying degrees of weathering in the riverbed. The research aims to examine the influence of iron distribution on the migration of cadmium originating from geological background sources. Based on the co-occurrence and co-migration characteristics of iron and cadmium in the study area, this research aims to reveal the migration features of geological background source cadmium by exploring the distribution patterns of iron sediment within rock fragments. By comparing sediment samples from both upstream and downstream river channels, we were able to identify three distinct stages that indicate the presence of cadmium originating from geological sources under weathering conditions: the unweathered stage; the intra-detritus migration stage, and the completely weathered stage; intra-detritus migration stage; and completely weathered stage. Furthermore, after leaving the Hetang formations shale outcrop area, there was a sharp decrease in content for Hetang formations detritus. At this point geological background source cadmium had largely escaped from constraints imposed by rock microstructure and had come into full contact with water. The findings from this research deepen our understanding regarding migration patterns for geological background source cadmium. They also reveal formation processes for geological background source cadmium pollution and provide a theoretical basis for identification and treatment for geological background source cadmium pollution.

Analysis of autogenic bifurcation processes resulting in river avulsion

Abstract. River bifurcations are constituent components of multi-thread fluvial systems, playing a crucial role in their morphodynamic evolution and the partitioning of water and sediment. Although many studies have been directed at exploring bifurcation dynamics, the conditions under which avulsions occur, resulting in the complete abandonment of one branch, are still not well understood. To address this knowledge gap, we develop a novel 1D numerical model based on existing nodal point relations for sediment partitioning, which allows for the simulation of the morphodynamic evolution of a free bifurcation. Model results show that when the discharge asymmetry is so high that the shoaling branch does not transport sediments (partial avulsion conditions) the dominant branch undergoes significant degradation, leading to a higher inlet step between the bifurcates and further amplifying the discharge asymmetry. The degree of asymmetry is found to increase with the length of the downstream channels to the point that when they are sufficiently long, the shoaling branch is completely abandoned (full avulsion conditions). To complement our numerical findings, we also formulate a new analytical model that is able to reproduce the essential characteristics of the partial avulsion equilibrium, which enables us to identify the key parameters that control the transition between different configurations. In summary, this research sheds light on the fundamental processes that drive avulsion through the abandonment of river bifurcations. The insights gained from this study provide a foundation for further investigations and may offer valuable information for the design of sustainable river restoration projects.

A Vision For Green, A Future For All

Future Green is committed to promoting green influence through market and marketing methods, connecting all upstream and downstream channels to truly provide sustainable green solutions. Our goal is to integrate the concepts of environmental protection into people's daily lives, allowing everyone to contribute to the protection of the Earth.

Electromagnetic Accessibility of Starlink User Terminals

The results of assessing the electromagnetic accessibility of radio emission sources of the Starlink satellite communication system are presented. The features of the organizational and technical structure of the system are considered. Its main technical capabilities for organizing the provision of telecommunications services to users of subscriber terminals are analyzed. The technical features of the infrastructure of the Starlink satellite communication system, which are essential for the electromagnetic accessibility of its sources, have been studied. The possibility of radio links for transmitting information content in the upstream and downstream channels of the Starlink satellite communication system was assessed. The main stages of the developed methodology for assessing the electromagnetic accessibility of sources are presented. Analytical expressions are given for calculating the probability of detection and attenuation of signals. The requirements for the sensitivity of the receiving equipment of monitoring equipment are substantiated. Dependences of the level of signal attenuation on the distance of control equipment were obtained.

Decreasing trends of downstream channel width: a comprehensive review of the scientific literature

Decreasing trends of downstream channel width: a comprehensive review of the scientific literature

The Influence of Hydraulic Characteristics on Structural Performance in a Pump-Turbine under No-Load Conditions

The operating state of a pump-turbine unit under no-load conditions is directly related to its safe and stable operation. In order to probe into the influence of hydraulic characteristics on structural performance, a pump-turbine assembled in China is selected for research by using CFD (computational fluid dynamics) and unidirectional FSI (fluid–structure interaction) methods. The vortex distribution and the law of pressure pulsation propagation are analyzed to capture the peculiar flow phenomena. The results show that the vortex distribution in the runner channel appears initially at the suction side of the blades but then propagates toward the pressure side with GVO. This produces rotating stall frequencies (0.7fn) and a combination of the RSI, asymmetry of the water ring in vaneless space, and high-amplitude pressure pulsations in the downstream channel close to the runner inlet and elbow section of the draft tube. This, in turn, is associated with the structural stress of the runner and guide vane. The stress level of the guide vane becomes alleviated under no-load conditions with large GVO, but the stress distribution of the runner is no longer symmetrical, which aligns with the vortex evolution in the runner passage. The stress concentration that develops further along the blade root increases the structural failure, which is also captured and verified as a crack in the prototype runner. The phenomena suggest that the RPT should avoid operating under no-load conditions with large GVO as far as possible. Therefore, in the design or optimization of the pump-turbine unit, the structures of the guide vanes and runner could be treated as a whole to investigate the resulting internal flow and structure characteristics.

Physical and numerical modeling of a landslide dam breach and flood routing process

Landslide dams are common hazards in mountainous regions, and outburst floods following dam breaches significantly impact people and infrastructure along rivers. The breaching processes associated with landslide dams and the interactions of outburst floods with channel terrain are very complex. The lack of event-based observations hampers the understanding of the mechanisms underlying these processes. Here, we combine a large-scale field experiment conducted on a natural river channel with a 3D numerical simulation. The numerical model was calibrated by a flume test and validated by experimental data from the large-scale physical model, demonstrating that it can accurately simulate the breaching process of landslide dams. The results show that the channel terrain controls the spatial and temporal distribution of the flow velocity and depth of the breach flood. The area of high flow velocity is mainly located in the block-confined narrow channel. In addition, outburst floods with high sediment concentrations led to extensive deposition in the downstream channels. The deposition thickness generally decreases in the flow direction and is affected by the channel topography. The measured changes in the particle size distribution of bed sediment before and after the dam breach support our findings. Moreover, the effect of upstream inflow on breach discharge was also investigated. It was found that the increasing inflow rate significantly accelerated the breaching process and increased the peak discharge. Our study enhances the understanding of the breach mechanism and reveals the connection between the hydrodynamic processes and geomorphic effects of breach floods, which can provide insight into the risk assessment of landslide dam hazards.

Accumulation and migration of particulate trace metals by artificial flood event of the Yellow River: From Xiaolangdi reservoir to estuary

Reservoir construction increasingly alters the natural transport of riverine water and sediment to the sea, including the trace metals and other pollutants. In 2018, an intensive flood event and 412 million tons of sediment were released from the Xiaolangdi dam during the water-sediment regulation of Yellow River, one of the world's largest sediment releases. During the artificial flood event, the surface sediments in Xiaolangdi Reservoir (XLD) and the Yellow River estuary, suspended sediments at Lijin Station were collected. The concentration and speciation of particulate Cr, Ni, Cu, Zn, Cd and Pb, as well as the major geochemical composition were analyzed, to characterize the behaviors of the metals from the reservoir to estuary mixing zone, and elucidate the controlling mechanisms. The results showed that for exogenous phases, Cr, Ni, Cu and Zn were likely bound to the FeMn oxides, whereas Pb and Cd were mainly adsorbed in the carbonates. The trace metals in XLD were stably combined with fine-grained bottom sediments at high concentrations before dam release. During the delivery from reservoir to downstream channel, the binding of Cr, Ni and Cu with FeMn oxides was markedly enhanced. Pb and Cu showed obvious migration from carbonates to FeMn oxides, and Cd and Pb were even released into the water. The accumulation and migration of trace metals were controlled by the adsorption of fine-grained components, especially FeMn oxides and carbonates, and influenced by the oxidizing processes. After entering the estuary, the trace metals were greatly scavenged by reservoir-sourced fine particles, tended to bound to organic matter affected by the reducing environment. Our results suggest that dam regulation and artificial flood events will likely alter the existing forms and redox state of trace metals and the potential environmental effects should be considered.

Modelling and numerical analysis of combined dual impact of SRS and FWM on the performance of upstream and downstream channels of a novel UDWDM/DWDM-PON

Wavelength division multiplexing (WDM) is an important method in modern optical communication systems for both long-haul and access networks. Particularly, after being standardized by Telecommunication Standardization Sector of International Telecommunication Union (ITU-T), WDM passive optical network (WDM-PON) becomes a considerable choice for fronthaul implementations of 5G networks. Stimulated Raman scattering (SRS) and four-wave mixing (FWM) are crucial nonlinear impacts that significantly limit the performance of WDM-based optical fiber communication systems. In this paper, an ultra-dense/dense WDM-PON (UDWDM/DWDM-PON) architecture has been proposed and combined dual impact of SRS and FWM on performances of upstream channels (USCs) and downstream channels (DSCs) of the proposed UDWDM/DWDM-PON has been numerically analyzed. USCs have been assumed to operate in 1310 nm region while operating wavelength region of DSCs has been taken as 1550 nm. Simulations have been performed on both UDWDM-PON architectures with 3.125 GHz and 6.25 GHz channel spacings and DWDM-PON architectures with channel spacings between 12.5 GHz and 100 GHz. 2x15-channel and 2x63-channel system structures have been considered in simulations. Signal-to-crosstalk ratio (SXR) has been chosen as performance parameter and its variation with channel input powers, channel spacings and channel lengths have been simulated for both USCs and DSCs of 2x15- and 2x63-channel UDWDM/DWDM-PONs. Results show that the proposed UDWDM/DWDM-PONs using single G.652 fiber can exhibit a reliable communication with minimum 23 dB SXR performance under combined dual impact of SRS and FWM unless some highlights about input powers and channel lengths mentioned in the paper are neglected.

Modelling flood frequency and magnitude in a glacially conditioned, heterogeneous landscape: testing the importance of land cover and land use

Abstract. A reliable flood frequency analysis (FFA) requires selection of an appropriate statistical distribution to model historical streamflow data and, where streamflow data are not available (ungauged sites), a regression-based regional flood frequency analysis (RFFA) often correlates well with downstream channel discharge to drainage area relations. However, the predictive strength of the accepted RFFA relies on an assumption of homogeneous watershed conditions. For glacially conditioned fluvial systems, inherited glacial landforms, sediments, and variable land use can alter flow paths and modify flow regimes. This study compares a multivariate RFFA that considers 28 explanatory variables to characterize variable watershed conditions (i.e., surficial geology, climate, topography, and land use) to an accepted power-law relationship between discharge and drainage area. Archived gauge data from southern Ontario, Canada, are used to test these ideas. Mathematical goodness-of-fit criteria best estimate flood discharge for a broad range of flood recurrence intervals, i.e., 1.25, 2, 5, 10, 25, 50, and 100 years. The log-normal, Gumbel, log-Pearson type III, and generalized extreme value distributions are found most appropriate in 42.5 %, 31.9 %, 21.7 %, and 3.9 % of cases, respectively, suggesting that systematic model selection criteria are required for FFA in heterogeneous landscapes. Multivariate regression of estimated flood quantiles with backward elimination of explanatory variables using principal component and discriminant analyses reveal that precipitation provides a greater predictive relationship for more frequent flood events, whereas surficial geology demonstrates more predictive ability for high-magnitude, less-frequent flood events. In this study, all seven flood quantiles identify a statistically significant two-predictor model that incorporates upstream drainage area and the percentage of naturalized landscape with 5 % improvement in predictive power over the commonly used single-variable drainage area model (p<2.2×10-16). Leave-one-out model testing and an analysis of variance (ANOVA) further support the parsimonious two-predictor model when estimating flood discharge in this low-relief landscape with pronounced glacial legacy effects and heterogeneous land use.

Barrier effect, blocking effect, and interference effect of electrohydrodynamic heat transfer enhancement in rectangular channels

This study investigates the heat transfer enhancement by electrohydrodynamic (EHD) in a rectangular channel with various geometric and operational parameters in a wide NEHD range of 0.4–5. Here, NEHD is a dimensionless number, describing the ratio of electrostatic, inertial, and viscous forces. Two working regimes in EHD are identified: inertial and electrostatic regimes. The results show that the barrier effect exists only in the inertial regime; however, the interference effect occurs in the electrostatic regime. The barrier effect relies heavily on NEHD rather than the dimensionless distance between electrodes l* and the dimensionless channel width H* because the thermal boundary layer cannot be disturbed sufficiently by a small electrostatic force; however, the interference effect depends on l* and H* rather than NEHD due to the stagnant area initiated only by a strong interaction between adjacent emitting electrodes. A new effect, the “blocking effect,” is found in the electrostatic regime. The mechanism of the blocking effect is different from that of the barrier and interference effects. The blocking effect is initiated by a giant vortex, which “blocks” the airflow flowing toward the downstream channel. The average Nusselt number of channels can be reduced by at least 8%, 13%, and 5% for the barrier, interference, and blocking effects, respectively. A working spectrum of EHD-induced heat transfer enhancement in a rectangular channel is provided under NEHD and channel area coupling conditions. We believe the spectrum can help in designing EHD-induced heat transfer enhancement because it provides theoretical guidance for avoiding the three effects.

Influence of Downstream Channel Width on Flow Features in a T-Shaped Open-Channel Confluence

Influence of Downstream Channel Width on Flow Features in a T-Shaped Open-Channel Confluence