The vibration of a single-degree-of-freedom bluff body positioned in water is intensified by the positive excitation, which is affected by the upstream interfering body and also by the downstream obstacles. This paper investigates the effect of downstream interference on galloping piezoelectric energy harvester performance. A mathematical model of the piezoelectric energy harvester under disturbance is established based on the extended Hamilton’s principle, and the theoretical output is further derived. The accuracy of proposed model is verified by the results of circulating water channel experiment. The effect of interference shape and approximate spacing (L/D) on system output is further analyzed, and the results show that the harvester performance is substantially improved when the distance between the bluff body and downstream interference is sufficiently small, and the interference is an elliptical cylinder with an aspect ratio of 0.6, i.e., the output power is 0.624 mW, which is improved by 24.39 % when L/D=1.8 and U=0.5m/s. Precise numerical calculations are further utilized to indicate that the harvester’s output performance is optimal when the downstream interference angle is 30°.