Future spacecraft missions aim to communicate with the Earth using near-infrared lasers. The possible bit rate of free-space optical communication (FSOC) is orders of magnitude greater when compared to current radio frequency transmissions. The challenge of ground-space FSOC is that atmospheric turbulence perturbs optical wavefront propagation. These wavefront aberrations can be measured using a Shack-Hartmann wavefront sensor (SHWFS). A ground-based adaptive optics (AO) system can mitigate these aberrations along the optical path by translating wavefront measurements into deformable mirror commands. However, errors result from atmospheric turbulence continuously evolving, and there are unavoidable delays during AO wavefront correction. The length of an acceptable delay is referred to as the coherence time-a parameter dependent on the strength of turbulence profile layers and their corresponding wind-driven velocity. This study introduces a novel technique, to the best of our knowledge, for using SHWFS single-source observations, e.g., the downlink signal from a geostationary satellite, to measure the strength and velocity of turbulence profile layers. This work builds upon previous research and demonstrates that single-source observations can disentangle turbulence profile layers through studying the cross-covariance of temporally offset SHWFS centroid measurements. Simulated data are used to verify that the technique can recover the coherence time. The expected and measured results have a correlation coefficient of 0.95.