Purpose : to study the dynamics of catalase activity in the blood and liver liver tissues with oxidative stress caused by the modeling of dyslipoproteinemia with the subsequent mechanical trauma of the skeletal muscle. Materials and methods . The study was conducted on white, unconventional sexual healthy rags of six months of age, weighing 200-230 grams, in the amount of 120 pieces contained in standard conditions of vivarium. All animals were divided into four groups, 30 animals in each group. Animals of the first group are intact rats to which no effects were applied. Animals of the second group (regeneration control) had a mechanical dissection in the area of the middle third of the calf muscle of the posterior limb. Animals of 1 and 2 groups were on the standard diet of Vivaria and had free access to water and food. The animals of the third and fourth groups were first modeled by dyslipoprotein for 63 days. All this time, animals received a high -carb and high -fat diet with a weight of dry substances increased up to 30 % and replacement of drinking water with a 20 % fructose solution. After the specified time, the animals of the fourth group were injured by the middle third of the calf muscle of the posterior limb. The activity of catalase was investigated in animals before the start of the experiment, as well as on 1, 3, 5, 14, 21 days of experience according to the standard methodology of Queen M. A. The capture of the material and the removal of rats from the experiment was carried out with accurate observance of all ethical norms applicable to laboratory animals . Under etheric anesthesia, a blood fence was made, and then the liver fence, which was washed with physiological solution and frozen. Then, by mechanical grinding of fabrics weighing 1 g and mixing with 9 ml of Tris Bufer (pH 7.4), homogenates were prepared in an automatic homogen at a speed of 5,000 rpm in a vessel with double walls, constantly cooled by running water. Results . The mechanical trauma of the calf muscle and dyslipoprotemia contributed to the intensification of oxidative processes at the local and systemic levels of the body, but the combination of these facts was characterized by extremely intense oxidative stress and led to a decrease in the activity of catalase in the blood and liver tissues.
Read full abstract