Laser spectroscopy is a powerful tool for performing engine diagnostics with high temporal and spatial resolution. The conventional technique for generating cycle-resolved measurements of species concentration is to collect data from independent engine cycles and to successively delay the laser shot from measurement to measurement to match the desired range of crank angles. However, due to cycle-to-cycle variations in combustion engines this approach will only produce either data sets of physically uncorrelated events or averaged results depending on data acquisition strategy. Ideally therefore measurement data should be recorded within a single cycle. Until now this has not been possible for species selective visualisation, owing to a lack of high power, high-speed tuneable laser sources and detectors. This paper presents true single cycle resolved measurements of fuel distribution, OH-distribution and chemiluminescence in a laboratory spark ignition (SI)-engine. A unique laser and detection system for high-speed imaging was used for this purpose. The laser source consists of four individual Nd : YAG lasers, whose beams are combined to provide a single output beam. Each laser can be operated in a double pulse mode, allowing for a maximum number of eight pulses to be produced per burst sequence. The time delay between the pulses can be arbitrarily varied from a few nanoseconds to 100 milliseconds. A framing camera, capable of recording eight images with maximum repetition rates of 100 MHz, was used as detector. Planar Laser Induced Fluorescence (PLIF) was used both for the fuel visualisation and for the OH measurements. 3-pentanone was used as tracer species for the fuel. The fundamental YAG-wavelength was quadrupled for excitation of 3-pentanone at 266 nm. Since it is rapidly pyrolised, 3-pentanone acts as a marker for unburned regions after onset of combustion. To investigate the changes of the reaction zone during a single cycle, OH fluorescence was used. For excitation at 283 nm a dye-laser, pumped by the Nd : YAG-cluster, was employed. The framing camera recorded the corresponding fluorescence at 309 nm. These images visualise the flame front as well as hot post flame regions. Integral aspects of flame propagation could be observed on line-of-sight chemiluminescence images, also recorded by the fast framing camera.