We investigate the composition of plasmas produced by laser ablation of metals with two time-delayed short laser pulses using fast imaging and time- and space-resolved optical emission spectroscopy. The ablated material is deposited on mica substrates and analyzed by atomic force microscopy. The laser-produced craters are inspected by optical microscopy to evaluate the ablated material quantity. It is shown that the fraction of nanoparticles in the ablation plume is strongly altered when a second laser pulse of sufficiently large delay is applied. Comparing the results obtained for different metals, we observe a significant nanoparticle reduction for interpulse delays of the order of the characteristic time of electron-lattice thermalization. More detailed analyses show that the plume changes occur on two different characteristic times, indicating two different mechanisms at its origin. Here, we discuss the involved processes and we propose a simple and efficient technique for the measurement of electron-lattice thermalization times based on plume observations during double pulse laser ablation.