Event Abstract Back to Event Proliferative potential of double positive CD4+/CD8+ T lymphocytes in coculture with tumor cells Florina M. Bojin1*, Luminita Cernescu1, Ada Cean1, Marius Toma1, Radu-Adrian Lazarovicz1, Laura Marusciac1, Gabriela Tanasie1, Carmen Panaitescu1 and Virgil Paunescu1 1 "Victor Babes" University of Medicine and Pharmacy Timisoara, Functional Sciences, Romania Immune response involved in tumor progression is not fully understood yet. It seems that patients with tumors develop mechanisms to escape the immune surveillance, leading to abnormal anti-tumoral immune response. The purpose of this study was to investigate in vitro behavior of peripheral blood mononuclear cells from healthy individuals when placed in contact with breast cancer cells (SKBR3). SKBR3 tumor cells were cultured in standard conditions and specific media. PBMCs were isolated from healthy donors using Ficoll density gradient and further cocultured with tumor cells for 5 days. Freshly isolated PBMCs were analyzed for expression of CD4, CD8, CD25, CD38, CD69, and HLA-DR. Adherent fraction and culture supernatant were harvested every day and analysed for the same phenotypical markers. Adherent SKBR3 were immunostained and flowcytometrically investigated. We found that CD45 positive initial population was approximately 70% and remained constant. CD4+ T cells decreased progressively from 70% to 46%, while CD8+ T cells maintained constant proportion of 18%. From day two of coculture we identified presence of double positive CD4+/CD8+ T cells population with a tendency to increase. CD25 and HLA-DR, late activation markers, presented an increased expression towards day 5 of experiment. SKBR3 cells proliferation was inhibited and aggressive markers expression was decreased. We may conclude that occurrence and proliferation of double positive CD4+/CD8+ T cells population when in contact with tumor antigens can be associated with anti-tumoral response of T lymphocytes. Acknowledgements PNII IDEI 318/2011 Keywords: CD4+CD8+ T cells, tumor cells, coculture, proliferative capacity, immunesuppresion Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Bojin FM, Cernescu L, Cean A, Toma M, Lazarovicz R, Marusciac L, Tanasie G, Panaitescu C and Paunescu V (2013). Proliferative potential of double positive CD4+/CD8+ T lymphocytes in coculture with tumor cells. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00404 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Florina M Bojin, "Victor Babes" University of Medicine and Pharmacy Timisoara, Functional Sciences, Timisoara, 300041, Romania, florinabojin@umft.ro Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Florina M Bojin Luminita Cernescu Ada Cean Marius Toma Radu-Adrian Lazarovicz Laura Marusciac Gabriela Tanasie Carmen Panaitescu Virgil Paunescu Google Florina M Bojin Luminita Cernescu Ada Cean Marius Toma Radu-Adrian Lazarovicz Laura Marusciac Gabriela Tanasie Carmen Panaitescu Virgil Paunescu Google Scholar Florina M Bojin Luminita Cernescu Ada Cean Marius Toma Radu-Adrian Lazarovicz Laura Marusciac Gabriela Tanasie Carmen Panaitescu Virgil Paunescu PubMed Florina M Bojin Luminita Cernescu Ada Cean Marius Toma Radu-Adrian Lazarovicz Laura Marusciac Gabriela Tanasie Carmen Panaitescu Virgil Paunescu Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
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