Double Peak Research Articles

Specificity of Key Sex Determination Genes in a Mammal with Ovotestes: The European Mole Talpa europaea.

Here, for the first time, the structure of genes involved in sex determination in mammals (full Sry and partial Rspo1, Eif2s3x, and Eif2s3y) was analyzed for the European mole Talpa europaea with ovotestes in females. We confirmed male-specificity for Eif2s3y and Sry. Five exons were revealed for Rspo1 and the deep similarity with the structure of this gene in T. occidentalis was proved. The most intriguing result was obtained for the Sry gene, which, in placental mammals, initiates male development. We described two exons for this canonically single-exon gene: the first (initial) exon is only 15 bp while the second exon includes 450 bp. The exons are divided by an extended intron of about 1894 bp, including the fragment of the LINE retroposon. Moreover, in chromatogram fragments, which correspond to intron and DNA areas, flanking both exons, we revealed double peaks, similar to heterozygous nucleotide sites of autosomal genes. This may indicate the existence of two or more copies of the Sry gene. Proof of copies requires an additional in-depth study. We hypothesize that unusual structure and possible supernumerary copies of Sry may be involved in ovotestes formation.

Six-channel colorimetric sensing of metal ions and advanced molecular information security based on fish scale-derived carbon–gold–silver nanocomposites

Due to its high flexibility and expansibility, nanomaterials-based systems have been constructed and applied from sensing, and medicine to molecular information technology. However, the current challenges include tedious and harsh nanomaterials preparation, the need for a wider range of response channels, new information paradigms, higher information density and length. Herein, fish-scale derived carbon nanomaterials (FSCN) were used as reducing and stabilizing agents for direct, green, and facile synthesis of carbon-bimetallic nanocomposites and applications in six-channel colorimetric sensing of Hg2+, and multi-combination and multi-round information encoding and protection for long text. First, the reaction sequence of three precursors (FSCN, Au3+, Ag+) was optimized to prepare FSCN–Au–Ag nanocomposites with double plasmonic absorption peaks of Au/Ag nanoparticles (NPs). The addition of Hg2+ causes the aggregation of nanoparticles and the formation of Ag/Hg amalgam or Au/Hg amalgam, FSCN–Au–Ag NPs were capable of multi-channel sensing (color, absorbance, and peak shift) of Hg2+. Especially, the detection limit of Hg2+ was 8.9 nM based on absorption response in wide wavelength range (456–546 nm), which was at least 6 times more sensitive than that at single wavelength. Moreover, different long text information can be encrypted and hidden in six-channel selective responses of FSCN–Au–Ag NPs-based colorimetric sensing system. By setting the threshold, these selective responses can be abstracted as a series of binary strings and then divided into shorter strings. Utilizing multi-combination and multi-round information coding, these short binary strings can be decrypted and merged into various long text content. This research promotes preparation and multi-purpose application of carbon-bimetallic nanomaterials, which provides new way to improve the sensing sensitivity of nanosystems and also offers new direction for molecular digitization.

Changes in the epidemiological characteristics of influenza in children in Zhengzhou, China, in the post-COVID-19 era

BackgroundInfluenza is a contagious respiratory disease posing a huge burden of disease for children around the world. The purpose of this study was to investigate the epidemiologic changes in childhood influenza in Zhengzhou, China, before, during, and after the COVID-19 outbreak. The aim of this study was to determine the impact of the COVID-19 outbreak and related prevention and control policies on the children’s influenza epidemiological trend.MethodsAll influenza report card data from the Children’s Hospital Affiliated with Zhengzhou University’s Disease Surveillance Reporting Management System were collected and analyzed monthly from January 2018 to December 2023. The period of the study was divided into three phases for comparison: the pre-pandemic period, the pandemic period, and the post-pandemic period.ResultsBetween January 2018 and December 2023, a total of 82,030 children with influenza were diagnosed at our hospital, including 46,453 males and 35,577 females. A total of 11,833 of them had to be hospitalized for influenza, and 321 of them were brought to the ICU. Influenza showed low-level epidemiologic status during the COVID-19 pandemic, and there was a substantial rise in influenza and a surge in the number of cases after the COVID-19 pandemic period. The year 2023 will had the most influenza cases (40,785). The peak incidence of influenza changes in 2022, from July to October, and in 2023, from February to April and from October to December. During the post-pandemic period, the proportion of new-borns and young children among influenza patients decreased, while the proportion of school-age children increased significantly, and the proportion of influenza patients hospitalized and the proportion of ICU admissions decreased.ConclusionInfluenza showed low-level epidemiologic status during the COVID-19 pandemic. In the post-pandemic period, there is a large increase in influenza incidence, with a double peak in influenza incidence. The proportion of school-age children with influenza has also increased. As a result, we recommend that influenza vaccination for key populations, particularly school-age children, be completed by October of each year in Henan Province, and that the government and schools increase education about nonpharmacological influenza prevention approaches.

A coupled thermo-mechanical model for investigating cracking and failure of composite interbedded rock

Composite interbedded rock is a special geological phenomenon that occurs when layers of rock (beds) of a given lithology lie between or alternate with other layers of a different lithology. The thermal and mechanical properties of the composite interbedded rocks exhibit strong anisotropy. Studying the thermomechanical coupling properties of natural composite interbedded rocks using existing experimental techniques is challenging. A coupled thermo-mechanical composite interbedded rock model was developed based on Fourier's heat-conduction law and Newton's second law. The thermodynamic responses and biaxial mechanical properties of composite interbedded rocks with different interlayer angles under the coupling of initial stress and thermal loading were studied for the first time. The results indicate that the thermal stresses within the granite interlayers are higher than those within the sandstone interlayers. Throughout the thermal loading process, thermal cracking occurred primarily within the (stiffer) granite interlayers. The number of thermal cracks generated during the cooling process far exceeds that during heating; at 400 °C, the number of thermal cracks during cooling is more than 35 times that during heating. When the interlayer dip angle approaches 90° and is aligned with the major principal stress direction, the phenomenon of the “double peaks” and layered failure becomes more pronounced. Within the range of variables considered, the relative impact of the three variables on peak stress in composite interbedded rock is as follows, from greatest to least: interlayer angle, thermal shock temperature, and model size. The results and developed modeling methods will contribute to the management and optimization of future engineering geology projects.

Authenticity Identification of F1 Hybrid Offspring and Analysis of Genetic Diversity in Pineapple

Breeding is an effective method for the varietal development of pineapple. However, due to open pollination, it is necessary to conduct authentic identification of the hybrid offspring. In this study, we identified the authenticity of offspring and analyzed the genetic diversity within the offspring F1 hybrids resulting from crosses between ‘Josapine’ and ‘MD2’ by single nucleotide polymorphism (SNP) markers. From the resequencing data, 26 homozygous loci that differentiate between the parents have been identified. Then, genotyping was performed on both the parents and 36 offspring to select SNP markers that are suitable for authentic identification. The genotyping results revealed that 2 sets of SNP primers, namely SNP4010 and SNP22550, successfully identified 395 authentic hybrids out of 451 hybrid offspring. We randomly selected two true hybrids and four pseudohybrids for sequencing validation, and the results have shown that two true hybrids had double peaks with A/G, while pseudohybrids had single peaks with base A or G. Further study showed that the identification based on SNP molecular markers remained consistent with the morphological identification results in the field, with a true hybridization rate of 87.58%. K-means clustering and UPGMA tree analysis revealed that the hybrid offspring could be categorized into two groups. Among them, 68.5% of offspring aggregated with MD2, while 31.95% were grouped with Josapine. The successful application of SNP marker to identify pineapple F1 hybrid populations provides a theoretical foundation and practical reference for the future development of rapid SNP marker-based methods for pineapple hybrid authenticity and purity testing.

LOV1 protein of Pseudomonas cichorii JBC1 modulates its virulence and lifestyles in response to blue light

Bacteria perceive light signals via photoreceptors and modulate many physiological and genetic processes. The impacts played by light, oxygen, or voltage (LOV) and blue light (BL) photosensory proteins on the virulence-related traits of plant bacterial pathogens are diverse and complex. In this study, we identified LOV protein (Pc-LOV1) from Pseudomonas cichorii JBC1 (PcJBC1) and characterized its function using LOV1-deficient mutant (JBC1Δlov1). In the dark state, the recombinant Pc-LOV1 protein showed an absorption band in UV-A region with a double peak at 340 nm and 365 nm, and within the blue-region, it exhibited a main absorption at 448 nm along with two shoulder peaks at 425 nm and 475 nm, which is a typical feature of oxidized flavin within LOV domain. The adduct-state lifetime (τrec) of Pc-LOV1 was 67.03 ± 4.34 min at 25 °C. BL negatively influenced the virulence of PcJBC1 and the virulence of JBC1Δlov1 increased irrespective of BL, indicating that Pc-LOV1 negatively regulates PcJBC1 virulence. Pc-LOV1 and BL positively regulated traits relevant to colonization on plant surface, such as adhesion to the plant tissue and biofilm formation. In contrast, swarming motility, exopolysaccharide production, and siderophore synthesis were negatively controlled. Gene expression supported the modulation of bacterial features by Pc-LOV1. Overall, our results suggest that the LOV photosensory system plays crucial roles in the adaptive responses and virulence of the bacterial pathogen PcJBC1. The roles of other photoreceptors, sensing of other wavelengths, and signal networking require further investigation.

Heme-catalyzed degradation of linoleate 9-hydroperoxide (9-HPODE) forms two allylic epoxy-ketones via a proposed pseudo-symmetrical diepoxy radical intermediate

Heme-initiated decomposition of unsaturated fatty acid hydroperoxides creates alkoxyl radicals that propagate a complex series of reactions to hydroxy, keto, epoxy and aldehydic products. Herein, among the products from the hematin-catalyzed degradation of 9-hydroperoxy-linoleic acid (9-HPODE), we observed a double peak on normal-phase HPLC that resolved on RP-HPLC into equal proportions of two epoxy-allylic ketones with identical UV spectra. Their proton NMR spectra were also indistinguishable and consistent with 9,10-trans-epoxy-11E-13-keto- and 9-keto-10E-12,13-trans-epoxy-octadecenoic acids. Acid hydrolysis to the corresponding dihydroxy-ketones and GC-MS analysis identified the earlier eluting product on RP-HPLC as the 9,10-epoxy regio-isomer. Starting from the C9-hydroperoxide, recovery of the two epoxy-ketones in equal proportions suggests their formation from a common intermediate. Earlier work has proposed formation of a pseudo-symmetrical diepoxy radical (9,10-epoxy-11(•)-12,13-epoxy, derived from an epoxy allylic hydroperoxide precursor) in the carbon chain fragmentation leading to aldehydic products. This intermediate in pathways of alkoxyl radical reactions forms equal pairs of aldehydes, and now also a pair of epoxy-ketones, and based on mechanism the same products arise from either 9-HPODE or 13-HPODE. Our results point to the intermediacy of this diepoxy-carbinyl radical in the origin of at least two classes of linoleate peroxidation products, and it should be considered as a viable intermediate for homo-conjugated diene peroxidation in general. The reactions could contribute to the aldehydes and epoxy-ketones in tissues undergoing oxidative transformations of polyunsaturated fatty acids.

Assessment of the impact of observations at Nainital (India) and Comilla (Bangladesh) on the CH4 flux inversion

Methane emission in South Asia is poorly understood due to a lack of observations, despite being a major contributor to methane emissions globally. We present the first results of atmospheric CH4 inversions using air samples collected weekly at Nainital, India (NTL), and Comilla, Bangladesh (CLA), in addition to surface background flask measurements by NOAA, CSIRO and AGAGE using the MIROC4-ACTM. Our simulations span from 2000 to 2020 (considering the fixed “edge” effect), but the main analysis period is 2013–2020, when both the NTL and CLA datasets are available. An additional flux uncertainty reduction of up to 40% was obtained (mainly in the northern part of the Indian subcontinent), which enhanced our confidence in flux estimation and reaffirmed the significance of observations at the NTL and CLA sites. Our estimated regional flux was 64.0 ± 4.7 Tg-CH4 yr−1 in South Asia for the period 2013–2020. We considered two combinations of a priori fluxes that represented different approaches for CH4 emission from rice fields and wetlands. By the inversion, the difference in emissions between these combinations was notably reduced due to the adjustment of the CH4 emission from the agriculture, oil and gas, and waste sectors. At the same time, the discrepancy in wetland emissions, approximately 8 Tg-CH4 yr−1, remained unchanged. In addition to adjusting the annual totals, the inclusion of NTL/CLA observations in the inversion analysis modified the seasonal cycle of total fluxes, possibly due to the agricultural sector. While the a priori fluxes consisted of a single peak in August, the a posteriori values indicated double peaks in May and September. These peaks are highly likely associated with field preparation for summer crops and emissions from rice fields during the heading stage (panicle formation). The newly incorporated sites primarily exhibit sensitivity to the Indo-Gangetic Plain subregion, while coverage in southern India remains limited. Expanding the observation network is necessary, with careful analysis of potential locations using back-trajectory methods for footprint evaluation.

Pore Structure Characterization and Fractal Characteristics of Tight Limestone Based on Low-Temperature Nitrogen Adsorption and Nuclear Magnetic Resonance

Pore structure characterization and fractal analysis have great significance for understanding and evaluating tight limestone reservoirs. In this work, the pore structure of tight limestone, low-temperature nitrogen adsorption (LTNA), and low-field nuclear magnetic resonance (NMR) are characterized, and the fractal dimension of the pore structure of tight limestone is discussed based on LTNA and NMR data. The results indicate that the pores of tight limestone have H3 and H4 types, the pore size distribution (PSD) of the H3 type is a wave distribution ranging from 2 to 10 nm, and the PSD of the H4 type is a unimodal distribution ranging from 2 to 10 nm. The transverse relaxation time (T2) spectrum of tight limestone shows a single peak (DF), double peak (SF), and triple peak (TF), and the ranges for the T2 spectra for micropores, mesopores, and macropores are 0.1 to 10 ms, 10 to 100 ms, and greater than 100 ms, respectively. The LTNA fractal dimension of tight limestone (DL) ranges between 2.4446 and 2.7688, with an average of 2.5729, and the NMR fractal dimensions of micropores (DNMR1), mesopores (DNMR2), and macropores (DNMR3) are distributed between 0.3744 and 1.1293, 2.4263 and 2.9395, and 2.6582 and 2.9989, respectively. Moreover, there is a negative correlation between DL and average pore radius, a positive correlation between DL and specific surface area, and a positive correlation between DNMR2 and DNMR3 and micropore content, while DNMR2 and DNMR3 are negatively correlated with the content of mesopores and macropores.

Diurnal variation of heart rate variability in individuals with spinal cord injury

BackgroundHeart rate variability (HRV) may provide objective information about cardiogenic autonomic balance in individuals with spinal cord injury (SCI). The aim of this study was to characterize the diurnal variation of HRV in individuals with SCI at lesion level T6 and above and lesion level below T6.MethodsThis was a retrospective analysis of a prior cross-sectional study. Individuals with chronic SCI underwent 24 h recording of the time between consecutive R waves (RR interval) to derive parameters of HRV as follows: standard deviation of all normal-to-normal R–R intervals (SDNN) and square root of the mean of the squared differences between successive R–R intervals (RMSSD) (time domain); and high frequency power (HF), low-frequency power (LF), very low frequency power (VLF), ultra-low frequency power (ULF) and total power (TP) (frequency domain). Changes in the magnitude of HRV outcomes over the 24 h period were investigated using a novel multi-component cosinor model constrained to the form of a three-harmonic Fourier series.ResultsParticipants were grouped as lesion level T6 and above (n = 22) or below T6 (n = 36). Most of them were male (n = 40, 69%) and the median age (interquartile range) was 50.5 (28) years. Both groups exhibited similar diurnal patterns in most HRV metrics. The lowest values occurred in the late afternoon (4–6 pm) and gradually increased, peaking around midnight to early morning (1–6 am). Exceptions included RMSSD, which peaked before midnight, and ULF, which showed a double peak pattern that peaked from 11 am to 1 pm and 4–6 am in participants with lesion level at T6 and above. The HRV values in participants with lesion level T6 and above were generally lower than participants with lesion level below T6, except for peak values of RMSSD, HF and LF.ConclusionThis study demonstrated substantial diurnal variation of HRV in participants with SCI in both groups of participants. In clinical and research settings, diurnal variations in HRV must be taken into consideration.

A unique wheel-shaped exposed core LSPR-PCF sensor for dual-peak sensing: Applications in the optical communication bands, M-IR region and biosensing

Photonic Crystal Fibers (PCF) effectiveness in practice decreases if the fabrication of the sensor becomes too complex. Keeping this in mind, we propose a one-of-a-kind wheel shaped PCF sensor with an exposed core containing only three air holes with exceptional sensing features. The sensor is equipped with dual plasmonic layers, Indium Tin Oxide (ITO, 10 % wt) and silver (Ag) with a coating of TiO2 to enhance the sensing capabilities and provide protection against oxidation. The sensor's distinctive configuration enables it to exhibit two distinct peaks within a range of refractive index from 1.32 to 1.38 for y-polarization and from 1.35 to 1.38 for x-polarization. The sensor specifications have been optimized to achieve the maximum levels of wavelength sensitivity (WS) and double peak shift sensitivity (DPSS). The sensor portrays a WS of 50,652 nm/RIU and the highest DPSS ever recorded, measuring 50,000 nm/RIU. Additionally, the sensor exhibits a significantly high scale of amplitude sensitivity (AS) of 1668.34 RIU−1 which is a very remarkable value considering silver as plasmonic material along with an outstanding figure of merit (FOM) of 1017.11 RIU−1. In addition, our sensor is able to manifest resolutions in the order of 10−6, demonstrating a resolution of 5.94 × 10−6 RIU with the deployment of amplitude interrogation method and 1.97 × 10−6 RIU with the wavelength interrogation method. The design spans an extensive spectrum, covering ultraviolet to mid-infrared wavelengths, enabling detection of biomolecules and biochemicals, along with operation in the optical communication band.

An ultra wide-band, high-sensitivity Q-band receiver for single-dish telescopes, eQ: Rest-frequency determination of CCS (JN = 43–32) and SO (JN = 10–01) and high-redshift CO (J = 1–0) detection

Abstract We report on the development and commissioning of a new Q-band receiver for the Nobeyama 45 m telescope, covering 30–50 GHz with a receiver noise temperature of about 15 K. We name it eQ (extended Q-band) receiver. The system noise temperatures for observations are measured to be ∼30 K at 33 GHz and ∼75 K at 45 GHz. The half-power beam-width (HPBW) is around 38${^{\prime \prime }}$ at 43 GHz. To enhance the observation capability, we tested the smoothed bandpass calibration technique and demonstrated that the observation time can be significantly reduced compared to the standard position switch technique. The wide-bandwidth capability of this receiver provides precise determination of rest frequencies for molecular transitions with an accuracy of a few kHz through simultaneous observations of multiple transitions. Particularly, we determined the rest frequency of SO (JN = 10–01) to be 30.001542 GHz, along with the rest frequency of CCS (JN = 43–32) being 45.379033 GHz, adopting CCS (JN = 32–21) at 33.751370 GHz as a reference line. The SO profile shows a double peak shape at the Cyanopolyyne Peak (CP) position of the Taurus Molecular Cloud-1 (TMC-1). The SO peaks coincide well with the CCS sub-components located near the outer parts of the TMC-1 filament. We interpret that the gravitational infall of TMC-1 generates shocks which enhance the SO abundance. The TMC-1 map shows that carbon-chain molecules are more abundant in the southern part of the filament, whereas SO is more abundant in the northern part. The eQ’s excellent sensitivity allowed us to detect faint CO (J = 1–0) spectra from the high-redshift object at a redshift of 2.442. Our receiver is expected to open new avenues for high-sensitivity molecular line observations in the Q-band.

Phase separation provides a mechanism to drive phenotype switching.

Phenotypic switching plays a crucial role in cell fate determination across various organisms. Recent experimental findings highlight the significance of protein compartmentalization via liquid-liquid phase separation in influencing such decisions. However, the precise mechanism through which phase separation regulates phenotypic switching remains elusive. To investigate this, we established a mathematical model that couples a phase separation process and a gene expression process with feedback. We used the chemical master equation theory and mean-field approximation to study the effects of phase separation on the gene expression products. We found that phase separation can cause bistability and bimodality. Furthermore, phase separation can control the bistable properties of the system, such as bifurcation points and bistable ranges. On the other hand, in stochastic dynamics, the droplet phase exhibits double peaks within a more extensive phase separation threshold range than the dilute phase, indicating the pivotal role of the droplet phase in cell fate decisions. These findings propose an alternative mechanism that influences cell fate decisions through the phase separation process. As phase separation is increasingly discovered in gene regulatory networks, related modeling research can help build biomolecular systems with desired properties and offer insights into explaining cell fate decisions.

Dependence of rain attenuation on rain rate and drop size distribution for Ka-band satellite communication over India

The Raindrop size distribution (DSD) is the most effective parameter to calculate rain-induced attenuation, which can be severe at Ka-band and higher frequencies. The authors have investigated the impact of rain rate and DSD on rain-induced specific attenuation over a tropical location Kolkata, India. DSD data from disdrometer has been used to calculate the specific rain attenuation with the help of Mie scattering theorem. Low to medium drop diameters (up to 3 mm) are the largest contributors to the various classes of rain rate and attenuation. Double peaks of drop sizes are significant for medium rain rate. The contribution of smaller drop diameters decrease, whereas the contribution of higher drop diameters increase with increasing rain attenuation. At lower rain attenuation, (up to 1.5 dB), complementary cumulative distribution function (CCDF) shows higher value for stratiform rain, with respect to convective rain. The higher attenuation occurrences are mostly observed during 12–18 Indian standard time (IST), i.e., in the afternoon. The results will be helpful for Ka-band (GSAT-14) and higher frequency satellite communication channel modeling over India.

Impact ionization double peaks analyzed in high temporal resolution on Solar Orbiter

Abstract. Solar Orbiter is equipped with electrical antennas performing fast measurements of the surrounding electric field. The antennas register high-velocity dust impacts through the electrical signatures of impact ionization. Although the basic principle of the detection has been known for decades, the understanding of the underlying process is not complete, due to the unique mechanical and electrical design of each spacecraft and the variability of the process. We present a study of electrical signatures of dust impacts on Solar Orbiter's body, as measured with the Radio and Plasma Waves electrical suite. A large proportion of the signatures present double-peak electrical waveforms in addition to the fast pre-spike due to electron motion, which are systematically observed for the first time. We believe this is due to Solar Orbiter's unique antenna design and a high temporal resolution of the measurements. The double peaks are explained as being due to two distinct processes. Qualitative and quantitative features of both peaks are described. The process for producing the primary peak has been studied extensively before, and the process for producing the secondary peak has been proposed before (Pantellini et al., 2012a) for Solar Terrestrial Relations Observatory (STEREO), although the corresponding delay of 100–300 µs between the primary and the secondary peak has not been observed until now. Based on this study, we conclude that the primary peak's amplitude is the better measure of the impact-produced charge, for which we find a typical value of around 8 pC. Therefore, the primary peak should be used to derive the impact-generated charge rather than the maximum. The observed asymmetry between the primary peaks measured with individual antennas is quantitatively explained as electrostatic induction. A relationship between the amplitude of the primary and the secondary peak is found to be non-linear, and the relation is partially explained with a model for electrical interaction through the antennas' photoelectron sheath.

Primitive Chain Network Simulations for Double Peaks in Shear Stress under Fast Flows of Bidisperse Entangled Polymers.

A few experiments have reported that the time development of shear stress under fast-startup shear deformations exhibits double peaks before reaching a steady state for bimodal blends of entangled linear polymers under specific conditions. To understand this phenomenon, multi-chain slip-link simulations, based on the primitive chain network model, were conducted on the literature data of a bimodal polystyrene solution. Owing to reasonable agreement between their data and our simulation results, the stress was decomposed into contributions from long- and short-chain components and decoupled into segment number, stretch, and orientation. The analysis revealed that the first and second peaks correspond to the short-chain orientation and the long-chain stretch, respectively. The results also implied that the peak positions are not affected by the mixing of short and long chains, although the intensity of the second peak depends on mixing conditions in a complicated manner.

Qualitative and Quantitative Detection of Typical Reproductive Hormones in Dairy Cows Based on Terahertz Spectroscopy and Metamaterial Technology.

Progesterone (PROG) and estrone (E1) are typical reproductive hormones in dairy cows. Assessing the levels of these hormones in vivo can aid in estrus identification. In the present work, the feasibility of the qualitative and quantitative detection of PROG and E1 using terahertz time-domain spectroscopy (THz-TDS) and metamaterial technology was preliminarily investigated. First, the time domain spectra, frequency domain spectra, and absorption coefficients of PROG and E1 samples were collected and analyzed. A vibration analysis was conducted using density functional theory (DFT). Subsequently, a double-ring (DR) metamaterial structure was designed and simulated using the frequency domain solution algorithm in CST Studio Suite (CST) software. This aimed to ensure that the double resonance peaks of DR were similar to the absorption peaks of PROG and E1. Finally, the response of DR to different concentrations of PROG/E1 was analyzed and quantitatively modeled. The results show that a qualitative analysis can be conducted by comparing the corresponding DR resonance peak changes in PROG and E1 samples at various concentrations. The best R2 for the PROG quantitative model was 0.9872, while for E1, it was 0.9828. This indicates that terahertz spectral-metamaterial technology for the qualitative and quantitative detection of the typical reproductive hormones PROG and E1 in dairy cows is feasible and worthy of in-depth exploration. This study provides a reference for the identification of dairy cow estrus.

An Electronic Structure Investigation of PEDOT with AlCl4- Anions-A Promising Redox Combination for Energy Storage Applications.

In this work, we use density functional theory to investigate the electronic structure of poly(3,4-ethylenedioxythiophene) (PEDOT) oligomers with co-located AlCl4- anions, a promising combination for energy storage. The 1980s bipolaron model remains the dominant interpretation of the electronic structure of PEDOT despite recent theoretical progress that has provided new definitions of bipolarons and polarons. By considering the influence of oligomer length, oxidation or anion concentration and spin state, we find no evidence for many of the assertions of the 1980s bipolaron model and so further contribute to a new understanding. No self-localisation of positive charges in PEDOT is found, as predicted by the bipolaron model at the hybrid functional level. Instead, our results show distortions that exhibit a single or a double peak in bond length alternations and charge density. Either can occur at different oxidation or anion concentrations. Rather than representing bipolarons or polaron pairs in the original model, these are electron distributions driven by a range of factors. Distortions can span an arbitrary number of nearby anions. We also contribute a novel conductivity hypothesis. Conductivity in conducting polymers has been observed to reduce at anion concentrations above 0.5. We show that at high anion concentrations, the energy of the localised, non-bonding anionic orbitals approaches that of the system HOMO due to Coulombic repulsion between anions. We hypothesize that with nucleic motion in the macropolymer, these orbitals will interfere with the hopping of charge carriers between sites of similar energy, lowering conductivity.

Identifying isotopes in HCl gas using high-resolution terahertz absorbance spectroscopy

This study presents a novel method for identifying the isotopes H35Cl and H37Cl using a unique high-resolution terahertz (THz) spectroscopy system. To prevent the absorbance of water vapor, chlorodimethylsilane (DMCS) was used as the source of HCl gas. Our analysis revealed periodic peaks at ∼1.9, 2.5, 3.1, 3.7, 4.4, 5.0, and 5.6 THz. Notably, we observed double peaks in the HCl isotopes H35Cl and H37Cl. The results were analyzed using least-square fitting, which showed that themeasurements agreed extremely well. The obtained peaks correlated with the rotational constants of HCl molecules, including 6B, 8B, 10B, 12B, 14B, 16B, and 18B.

Gravitational waves from phase transitions and cosmic strings in neutrino mass models with multiple majorons

We explore the origin of Majorana masses within the majoron model and how this can lead to the generation of a distinguishable primordial stochastic background of gravitational waves. We first show how in the simplest majoron model only a contribution from cosmic string can be within the reach of planned experiments. We then consider extensions containing multiple complex scalars, demonstrating how in this case a spectrum comprising contributions from both a strong first order phase transition and cosmic strings can naturally emerge. We show that the interplay between multiple scalar fields can amplify the phase transition signal, potentially leading to double peaks over the wideband sloped spectrum from cosmic strings. We also underscore the possibility of observing such a gravitational wave background to provide insights into the reheating temperature of the universe. We conclude highlighting how the model can be naturally combined with scenarios addressing the origin of matter of the universe, where baryogenesis occurs via leptogenesis and a right-handed neutrino plays the role of dark matter.