Double Line Research Articles

The Hilbert function of general unions of lines and double lines in the projective space

We study the Hilbert function of a general union X⊂P3 of x double lines and y lines. In many cases (e.g. always for x=2 and y≥3 or for x=3 and y≥2 or for x≥4 and y≥⌈((3x+43)−27x−12)/(3x+2)⌉+3−x) we prove that X has maximal rank. We give a few examples of x and y for which X has not maximal rank.

Assessment of photovoltaic generation penetration effect on the maximum loadability of the system

This paper proposed a method to investigate the effect of increasing PV penetration on the voltage stability of an IEEE 14-bus test system considering maximum PV penetration and system loadability limit. The critical bus of the test system has been decided based on nose curve analysis. The solar PV system is deployed with the most critical bus of the system. The effect of increasing PV penetration on the improvement in the loading capacity of various power system components like transmission lines, transmission line transformers, and generators is investigated over the adopted test system. Based on solar PV penetration up to 100 MW, the maximum loadability limit of the IEEE 14-bus test system increases, as shown by the results. Bus 14 is found to be the most severe bus of the test system using the continuation power flow (CPF) method. However, in some cases, overloading situations develop after a certain limit of PV penetration in the power system. In this condition, the overloading of the power system equipment is improved by the use of a Static Synchronous Compensator (STATCOM) at bus number 14, a double circuit line in the critical line (9–14), and Static Synchronous Series Compensator (SSSC) in the most severe line (9–14) in the system. The maximum loadability of the system gets maximum enhanced from 4.0349 p.u. to 4.5602 p.u. under simultaneous use of solar PV generation, SSSC, and STATCOM at the most critical bus and in most severe line of the system. As evidence, the enhancement in maximum loadability of the system found using the proposed method has been also compared with the existing research. The maximum system loadability has been also enhanced under normal and (N-1) contingency conditions by the use of PV penetration in the system. PSAT/MATLAB software is used for simulation and maximum loadability has been investigated by continuation power flow (CPF) method.

Highly efficient relay triggering circuit for fault detection during Power swings

This paper introduces a Discrete Wavelet Transform based very simple and fast acting algorithm with multi-resolution analysis to sense all types of faults in presence of power swings using current signal analyzation. The algorithm confirms very quick and efficient detection of various fault types in the first signal decomposition level of signal. The novelty of proposed algorithm lies in use of special type of Battle Lemarie mother wavelet having an advantage of perfect symmetry ensuring decomposition into B-Spline or same order polynomials capturing excellent speed and time-frequency localization of signal. The algorithm is tested for different fault parameters such as fault resistance, fault distance and time of initiation of faults considering EHV double circuit transmission line network and IEEE 9 Bus system developed in MATLAB environment. The proposed algorithm is capable of detecting all types of faults consistently within minimum time of 0.001 sec.

HAND factors regulate cardiac lineage commitment and differentiation from human pluripotent stem cells

BackgroundTranscription factors HAND1 and HAND2 (HAND1/2) play significant roles in cardiac organogenesis. Abnormal expression and deficiency of HAND1/2 result in severe cardiac defects. However, the function and mechanism of HAND1/2 in regulating human early cardiac lineage commitment and differentiation are still unclear.MethodsWith NKX2.5eGFP H9 human embryonic stem cells (hESCs), we established single and double knockout cell lines for HAND1 and HAND2, respectively, whose cardiomyocyte differentiation efficiency could be monitored by assessing NKX2.5-eGFP+ cells with flow cytometry. The expression of specific markers for heart fields and cardiomyocyte subtypes was examined by quantitative PCR, western blot and immunofluorescence staining. Microelectrode array and whole-cell patch clamp were performed to determine the electrophysiological characteristics of differentiated cardiomyocytes. The transcriptomic changes of HAND knockout cells were revealed by RNA sequencing. The HAND1/2 target genes were identified and validated experimentally by integrating with HAND1/2 chromatin immunoprecipitation sequencing data.ResultsEither HAND1 or HAND2 knockout did not affect the cardiomyocyte differentiation kinetics, whereas depletion of HAND1/2 resulted in delayed differentiation onset. HAND1 knockout biased cardiac mesoderm toward second heart field progenitors at the expense of first heart field progenitors, leading to increased expression of atrial and outflow tract cardiomyocyte markers, which was further confirmed by the appearance of atrial-like action potentials. By contrast, HAND2 knockout cardiomyocytes had reduced expression of atrial cardiomyocyte markers and displayed ventricular-like action potentials. HAND1/2-deficient hESCs were more inclined to second heart field lineage and its derived cardiomyocytes with atrial-like action potentials than HAND1 single knockout during differentiation. Further mechanistic investigations suggested TBX5 as one of the downstream targets of HAND1/2, whose overexpression partially restored the abnormal cardiomyocyte differentiation in HAND1/2-deficient hESCs.ConclusionsHAND1/2 have specific and redundant roles in cardiac lineage commitment and differentiation. These findings not only reveal the essential function of HAND1/2 in cardiac organogenesis, but also provide important information on the pathogenesis of HAND1/2 deficiency-related congenital heart diseases, which could potentially lead to new therapeutic strategies.

Ballast-Supported Foundation Designs for Low-Cost Open-Source Solar Photovoltaic Racking

Although solar photovoltaic (PV) system costs have declined, capital cost remains a barrier to widespread adoption. Do-it-yourself (DIY) system designs can significantly reduce labor costs, but if they are not attached to a building structure, they require ground penetrating footings. This is not technically and economically feasible at all sites. To overcome these challenges, this study details systems designed to (1) eliminate drilling holes and pouring concrete, (2) propose solutions for both fixed and variable tilt systems, (3) remain cost effective, and (4) allow for modifications to best fit the user’s needs. The ballast-supported foundations are analyzed for eight systems by proposing two separate ballast designs: one for a single line of post systems, and one for a double line of post systems, both built on a 4-kW basis. The results of the analysis found that both designs are slightly more expensive than typical in-ground concrete systems by 25% (assuming rocks are purchased at a landscaping company), but the overall DIY system’s costs remain economically advantageous. Sensitivity analyses are conducted to show how modifications to the dimensions influence the weight of the system and thus change the economic value of the design, so users can trade dimensional freedom for cost savings, and vice versa. Overall, all wood-based PV racking system designs provide users with cost-effective and easy DIY alternatives to conventional metal racking, and the novel ballast systems presented provide more versatility for PV systems installations.

Simulation Analysis of Electric Field on the Body Surface of Personnel Engaged in Interphase Live Working on 750 kV Double Circuit Transmission Lines on the Same Tower

This article focuses on the assistance of electric lifting devices for phase-to-phase live working. Taking a typical 750 kV double circuit vertical arrangement line on the same tower as the research object, a three-dimensional simulation model of a real tower conductor simulated human is established. The field strength on the human body surface is calculated and analyzed during the process of operators entering the equipotential (conductor) while riding the electric lifting device in order to determine the risk points of the operation and determine the safety protection measures for the human body. Through simulation, it is found that when simulating human entry (exit) and phase-down operations, the human body is located at different distances of 0.4 m-1.5 m directly below the conductor. When not wearing shielding clothing, the maximum field strength of the human head is much greater than the allowable value. If using shielding clothing with a shielding efficiency of 60 dB for safety protection, the surface field strength inside the worker’s clothing can be reduced to 2.25 kV/m, meeting the requirements of live working. Operators should pay attention to the electric field protection at each phase wire when using an electric lifting device to enter (exit) the interphase wire. Equipotential operators must wear a complete set of shielding clothing for 750 kV live working.

Tobacco NtUBC1 and NtUBQ2 enhance salt tolerance by reducing sodium accumulation and oxidative stress through proteasome activation in Arabidopsis

The ubiquitin/proteasome system plays a crucial role in the regulation of plant responses to environmental stress. Here, we studied the involvement of the UBC1 and UBQ2 genes encoding a ubiquitin conjugating enzyme (E2) and ubiquitin extension protein, respectively, in the response to salt stress. Our results showed that the constitutive expression of tobacco NtUBC1 and NtUBQ2 in Arabidopsis thaliana improved salt tolerance, along with the lower Na+ level and higher K+/Na+ ratio compared to control plants. Moreover, the expression levels of sodium transporters, including AtHKT1 (High-Affinity K+ Transporter1) and AtSOS1 (Salt Overly Sensitive 1), were higher in NtUBC1- and NtUBQ2-Arabidopsis. However, the transcript level of AtNHX1 (Na+/H+ Exchanger 1) was similar between control and transgenic plants. After salt exposure, the activity of the 26S proteasome markedly increased in NtUBC1- and NtUBQ2-expressing plants; however, ubiquitinated protein levels decreased compared to control plants. Furthermore, higher activity of antioxidant enzymes and lower ROS production were observed in UBC1- and UBQ2-expressing plants. We further challenged atubc1, atubc2, and atubq2 single mutants and atubc1ubc2 double mutant lines with salt stress; interestingly, the salt sensitivity and sodium levels of the studied mutants were enhanced, while the potassium levels were reduced. However, the atubc1ubc2 double mutant illustrated a more severe phenotype than the single mutants, probably due to the redundant function of UBC1 and UBC2 in Arabidopsis. Taken together, NtUBC1 and NtUBQ2 enhance salt tolerance by enhancing 26S proteasome activity and reducing Na+ accumulation, ROS, and ubiquitinated/salt-denatured proteins.

CLLC Modeling and Control in V2G Mode to Mitigate Double-Line Frequency Current for High-Power Density On-Board Charger

CLLC Modeling and Control in V2G Mode to Mitigate Double-Line Frequency Current for High-Power Density On-Board Charger

Zinc white marker paint in Mondrian’s neoplastic paintings

This research provides new insights into the composition of zinc white paints used by Piet Mondrian during his neoplastic period. Nine paintings, dated between 1921 and 1935, were studied, with a focus on three works in the collection of the Fondation Beyeler (Basel, Switzerland)—Tableau I (1921–1925), Composition with yellow and blue (1932) and Composition with double line and blue (1935)—and on Lozenge composition with yellow lines (1933) in Kunstmuseum Den Haag (The Netherlands). Cross sections from other paintings, most of which previously studied by Van Asperen de Boer in the early 1990’s, were reexamined as well. The analyses revealed a zinc white paint with aluminum phosphate inclusions (ZW-Al/P). In two of the works, a zinc white paint with aluminum sulphate was also found. The occurrence of aluminum phosphate (or aluminum sulphate) in 1920–1930’s paint formulations, and in paintings by Mondrian, has never been reported so far. Likely, the use of this zinc white paint in Mondrian’s and other artworks is more widespread than currently known, but it may have been overlooked in similar case studies, since its identification can only be accomplished with detailed scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM–EDX) analysis of cross sections. Mondrian’s use of pure ‘bright’ ZW-Al/P paint is limited to the later paintings (1932–1935) of this study, whereas in the earlier works (1921–1929) it was mostly mixed with lead white, likely conveying a different hue. Both the ZW-Al/P and Zn-Al/S ‘marker paints’ were also added to yellow, blue or black paint in specific paint layers of the planes and lines, respectively. It was also shown that Mondrian used the same paint on the frames as in the white planes and that the frames were painted while still working on the composition, and not only when he considered the work completed. Indeed, the detection of the zinc white marker paints might help to delineate the chronology of Mondrian’s working process in other paintings and may also be important in the interpretation of overpaints in the course of a treatment when removal of certain paint layers is considered.

DISC1 and reelin interact to alter cognition, inhibition, and neurogenesis in a novel mouse model of schizophrenia.

The etiology of schizophrenia (SCZ) is multifactorial, and depending on a host of genetic and environmental factors. Two putative SCZ susceptibility genes, Disrupted-in-Schizophrenia-1 (DISC1) and reelin (RELN), interact at a molecular level, suggesting that combined disruption of both may lead to an intensified SCZ phenotype. To examine this gene-gene interaction, we produced a double mutant mouse line. Mice with heterozygous RELN haploinsufficiency were crossed with mice expressing dominant-negative c-terminal truncated human DISC1 to produce offspring with both mutations (HRM/DISC1 mice). We used an array of behavioral tests to generate a behavioral phenotype for these mice, then examined the prefrontal cortex and hippocampus using western blotting and immunohistochemistry to probe for SCZ-relevant molecular and cellular alterations. Compared to wild-type controls, HRM/DISC1 mice demonstrated impaired pre-pulse inhibition, altered cognition, and decreased activity. Diazepam failed to rescue anxiety-like behaviors, paradoxically increasing activity in HRM/DISC1 mice. At a cellular level, we found increased α1-subunit containing GABA receptors in the prefrontal cortex, and a reduction in fast-spiking parvalbumin positive neurons. Maturation of adult-born neurons in the hippocampus was also altered in HRM/DISC1 mice. While there was no difference in the total number proliferating cells, more of these cells were in immature stages of development. Homozygous DISC1 mutation combined with RELN haploinsufficiency produces a complex phenotype with neuropsychiatric characteristics relevant to SCZ and related disorders, expanding our understanding of how multiple genetic susceptibility factors might interact to influence the variable presentation of these disorders.

Magneto-thermoelastic coupled resonance characteristics of a rotating functionally graded ferromagnetic cylindrical shell under double harmonic line loads

The magneto-thermoelastic coupled resonance of a rotating functionally graded (FG) ferromagnetic cylindrical shell subjected to double harmonic line loads in magnetic and temperature fields is investigated. In accordance with Donnell's thin shell theory on the physical neutral surface, the nonlinear geometric equations are determined. Utilizing the thermoelastic constitutive relationships, combining with the gradient characteristics and temperature dependence of physical parameters, the governing equations of kinetic energy and strain energy are given. Based on the magnetoelasticity theory, and considering the nonlinear magnetization of ferromagnetic metal, the calculation formulas of Lorentz force and magnetizing force acting on the shell are deduced. The magneto-thermoelastic coupled dynamic equations are derived by applying Hamilton principle and discretized by Galerkin method. Subsequently, considering the different resonance forms, the multiple scales method and Lyapunov stability theory are employed to obtain the theoretical solutions and stability discriminants. Numerical examples are carried out to plot the characteristic diagrams with different parameters, which are used to analyze the characteristics of nonlinear coupled resonance. Furthermore, the primary resonance and combination resonance are selected to analyze the bifurcation and chaotic behavior. Results show that the variation of different parameters affects obviously the resonance amplitude and stability. The changes of amplitude and position of hard excitation can lead to resonance region drift. Amplitude, frequency, and position of excitation have significant effects on bifurcation and chaos. The motion state of cylindrical shell is transmitted from periodic response to chaotic response through the accumulation of period-doubling bifurcation.

Xenophagy receptors Optn and p62 and autophagy modulator Dram1 independently promote the zebrafish host defense against Mycobacterium marinum.

Anti-bacterial autophagy, also known as xenophagy, is a crucial innate immune process that helps maintain cellular homeostasis by targeting invading microbes. This defense pathway is widely studied in the context of infections with mycobacteria, the causative agents of human tuberculosis and tuberculosis-like disease in animal models. Our previous work in a zebrafish tuberculosis model showed that host defense against Mycobacterium marinum (Mm) is impaired by deficiencies in xenophagy receptors, optineurin (Optn) or sequestome 1 (p62), and Damage-regulated autophagy modulator 1 (Dram1). However, the interdependency of these receptors and their interaction with Dram1 remained unknown. In the present study, we used single and double knockout zebrafish lines in combination with overexpression experiments. We show that Optn and p62 can compensate for the loss of each other's function, as their overexpression restores the infection susceptibility of the mutant phenotypes. Similarly, Dram1 can compensate for deficiencies in Optn and p62, and, vice versa, Optn and p62 compensate for the loss of Dram1, indicating that these xenophagy receptors and Dram1 do not rely on each other for host defense against Mm. In agreement, Dram1 overexpression in optn/p62 double mutants restored the interaction of autophagosome marker Lc3 with Mm. Finally, optn/p62 double mutants displayed more severe infection susceptibility than the single mutants. Taken together, these results suggest that Optn and p62 do not function downstream of each other in the anti-mycobacterial xenophagy pathway, and that the Dram1-mediated defense against Mm infection does not rely on specific xenophagy receptors.

Flashover pattern analysis for 275 kV double circuit transmission lines during direct lightning strikes

Lightning overvoltage causes outages of transmission lines (TLs) in tropical countries. Identifying and analyzing the key factors responsible for tripping the lines can improve the performance of the lines. In this work, the flashover patterns due to direct lightning strikes on 275 kV double circuit TLs and towers in Malaysia was evaluated with Electromagnetic Transient Program (EMTP-RV). Three parameters that include lightning current magnitude, power frequency angle and tower footing resistance were analyzed on the line performance during direct lightning strikes. The simulated results found that two key parameters, lightning current at 270 kA and footing resistance at 50 Ω, reveal the highest impact on the insulator flashover. This finding was also verified from multivariate analysis results which is rarely found in the previous works in this field. As a key contribution in this work and in validation to the model, the simulated fault waveform patterns and voltage magnitudes were compared with the actual site data obtained from six different locations in Peninsular Malaysia. The analyzed results show a similar waveform pattern with an overall average voltage magnitude difference of 6.0 % before and after fault. The model is useful to electric utilities for analyzing transient performance of TLs.

Recurrent Squamous Cell Carcinoma of the Upper Eyelid Treated With Combination Therapy of ALA Photodynamic Therapy and Surgery With Secondary Healing

Background. Cutaneous squamous cell carcinoma (cSCC) frequently occurs in photoexposed areas. Surgery remains the mainstay of treatment in attempts to reduce recurrence, but it must be combined with other therapy because of the limited excision possible in the region of the eyelid, lip, and nose. Photodynamic therapy (PDT) is a relatively new treatment modality that involves the administration of a photosensitizing drug and its subsequent activation by specific wavelengths of light to produce reactive oxygen species that specifically destroy target cells. Case Report. An 87-year-old female presented 4 weeks after initial resection with recurrent medium-differentiated cSCC measuring 5.2 cm × 3 cm × 2 cm in the left upper eyelid. Subsequent treatment involved palliative resection with an additional 1 cm at 3 margins of the tumor (excluding the bottom edge of the double eyelid line) and 3 applications of PDT using 5-aminolevulinic acid as the photosynthesizing agent in the open wound over a 2-week period. The wound healed well within 6 weeks. During the following 4 years, the patient showed satisfactory progress in both aesthetics and function, with no sign of recurrence or metastasis. Conclusion. Refractory cSCC was successfully managed using a combination of PDT and secondary healing, and functions of the head and face were well protected. These results suggest that such management warrants consideration in clinical settings.

Targeting FGFR3 signaling and drug repurposing for the treatment of SLC26A2-related chondrodysplasia in mouse model

BackgroundMutations in Slc26a2 cause a spectrum of autosomal-recessive chondrodysplasia with a significant and negligible influence on the quality of life. It has been reported that Slc26a2 deficiency triggers the ATF6 branch of the UPR, which may, in turn, activate the negative regulator of the FGFR3 signaling pathway. However, the correlation between the deletion of Slc26a2 and the augmentation of downstream phosphorylation of FGFR3 has not been investigated in vivo. MethodsFirst, we constructed Slc26a2 and Fgfr3 double knockout mouse lines and observed gross views of the born mice and histological staining of the tibial growth plates. The second approach was to construct tamoxifen-inducible Cre-ERT2 mouse models to replicate SLC26A2-related non-lethal dysplastic conditions. Pharmacological intervention was performed by administering the FGFR3 inhibitor NVP-BGJ398. The effect of NVP-BGJ398 on chondrocytes was assessed by Alcian blue staining, proliferation, apoptosis, and chondrocyte-specific markers and then verified by western blotting for variations in the downstream markers of FGFR3. The growth process was detected using X-rays, micro-CT examination, histomorphometry staining of growth plates, and immunofluorescence. ResultsGenetic ablation of Fgfr3 in embryonic Slc26a2-deficient chondrocytes slightly attenuated chondrodysplasia. Subsequently, in the constructed mild dysplasia model, we found that postnatal intervention with Fgfr3 gene in Slc26a2-deficient chondrocytes partially alleviated chondrodysplasia. In chondrocyte assays, NVP-BGJ398 suppressed the defective phenotype of Slc26a2-deficient chondrocytes and restored the phosphorylation downstream of FGFR3 in a concentration-dependent manner. In addition, in vivo experiments showed significant alleviation of impaired chondrocyte differentiation, and micro-CT analysis showed a clear improvement in trabecular bone microarchitectural parameters. ConclusionOur results suggested that inhibition of FGFR3 signaling pathway overactivation and NVP-BGJ398 has promising therapeutic implications for the development of SLC26A2-related skeletal diseases in humans. The translational potential of this articleOur data provide genetic and pharmacological evidence that targeting FGFR3 signaling via NVP-BGJ398 could be a route for the treatment of SLC26A2-associated skeletal disorders, which promisingly advances translational applications and therapeutic development.

Long-term Effects of Irrigation with Treated Municipal Wastewater on Soil Chemical and Physical Responses in Commercial Vineyards in the Coastal Region of South Africa

The prolonged drought in the Western Cape province of South Africa in recent years has been particularly detrimental to the wine industry. Water restrictions imposed by authorities and the limited supply of fresh water that can be stored, have emphasisedthe need for alternative water sources for vineyard irrigation. Treated municipal wastewater has been used successfully as an alternative source of irrigation water in other countries. A long-term trial was conducted in commercial vineyards in the Coastal region of South Africa to assess the impact of treated municipal wastewater irrigation on vineyards. Cabernet Sauvignon and Sauvignon blanc grapevines were irrigated using treated municipal wastewater from the Potsdam wastewater treatment works for 11 years. Grapevines were either rainfed (RF), irrigated with treated municipal wastewater via a single dripper line (SLD), or received twice the volume of wastewater via a double dripper line (DLD). Irrigation using treated municipal wastewater increased soil pH(KCl), the electrical conductivity of the saturated extract (ECe) and Cl-. Substantial amounts of Na+ and K+ accumulated in the topsoil due to irrigation with treated municipal wastewater. These soil K+ increases could have a negative effect on wine colour stability should the levels of soil K+ be such that they are absorbed excessively by grapevines. The near-saturation hydraulic conductivity (Kns) at the surface of the soil could be related to the ECe in the topsoil. The results represent specific in-field situations in three commercial vineyards under one set of climatic conditions.

Settlement rule of underpass railway model in shield tunnel and influence of nanomaterials on slurry performance during construction

In the shield tunnel, the phenomenon of structure penetration occurs frequently. Therefore, strict control of ground subsidence is imperative during the construction phase. In addition, other variables may be influence the performance of the slurry during the grouting procedure. This study aims to investigate and analyze the impact of nanomaterials on the properties of slurry used in construction, with the goal of determining the optimal construction route for the shield, ensuring quality construction of a U-shaped section, and meeting settlement control requirements. Firstly, the impact of double line spacing on the subsidence of U-shaped grooves is analyzed. Subsequently, the influence of common nanomaterials on slurry properties is examined through compressive strength testing. Among these nanomaterials, micro-silica powder exhibits the most significant enhancement effect on construction slurry performance with an optimal content of 5%. If the content of silica powder reaches 5%, the initial strength and later strength of the cement slurry will increase by 35% and 25% respectively, thereby promoting the enhanced performance of the slurry. In this study, the settlement of a U-shaped groove on the surface and subgrade is controlled by the settlement of a shield tunnel underpass railway model based on the distance between shield construction lines, providing valuable insights for future constructions with similar characteristics.

Cytogenetic and agro-morphological study of Wheat addition lines possessing Thinopyrum elongatum and Aegilops triuncialis chromosomes

Novel genetic variation can be induced by introgression of alien chromosomes in wheat for which wheat-alien addition lines carrying 45 chromosomes are instrumental. In the course of developing wheat addition lines with 45 chromosomes, two different parental lines Wheat-Thinopyrum elongatum disomic addition lines (Wheat-Th. DALs; 2n=44)) and Wheat-Th. elongatum- Aegilops triuncialis 3CSAT double monosomic addition lines (Wheat-Th. DMALs; 2n=44) need to cross. The present study mainly focuses on the effects of chromosomal additions in wheat background by comparing the morphological and agronomic traits of the parental lines. Both the parental lines were reconfirmed 2n=44 cytologically by using squash technique. Pollen fertility was highest (75%) in Wheat-3CSAT-6E DMAL and lowest (44.73%) in Wheat-5E DAL, whereas, spike length was longest (8.71 cm) in Wheat-3CSAT-3E DMAL and shortest (5.92 cm) in Wheat-1E DAL. Plant height was highest (97.6 cm) in Wheat-3CSAT-5E DMAL and lowest (55.33 cm) in Wheat-7E DAL. The plant height of DMALs always exceeded that of DALs. Furthermore, seed set was highest (31 seeds per spike) in Wheat-7E DALs and lowest (13 seeds per spike) in addition lines disomic for 4E chromosomes. The effect of different alien chromosomes in wheat is discussed in this paper.

Dynamic Investigations of Grid Connected Fixed-Speed Wind Turbine During Grid Faults

Especially during grid faults, grid code requirements for wind power integration have become a key element in improving grid efficiency and reliability. Under severe problems for network operation, wind turbines are expected to continue operating and supporting the grid during frequency restoration. This paper presents simulation results of a fixed-speed grid-connected wind turbine under various short-circuit current contributions. Fault analysis is carried out by studying the grid side line to ground fault, double line fault, double line to ground fault, and three phase fault involving ground and without ground. The obtained current waveforms are analyzed to explain the behavior, such as the rate of decay and peak values. Variations of active and reactive power during post-fault conditions and faulty conditions are investigated. Moreover, recommendations for switchgear and protection equipment are performed.

Identifying and Characterizing Candidate Genes Contributing to a Grain Yield QTL in Wheat.

The current study focuses on identifying the candidate genes of a grain yield QTL from a double haploid population, Westonia × Kauz. The QTL region spans 20 Mbp on the IWGSC whole-genome sequence flank with 90K SNP markers. The IWGSC gene annotation revealed 16 high-confidence genes and 41 low-confidence genes. Bioinformatic approaches, including functional gene annotation, ontology investigation, pathway exploration, and gene network study using publicly available gene expression data, enabled the short-listing of four genes for further confirmation. Complete sequencing of those four genes demonstrated that only two genes are polymorphic between the parental cultivars, which are the ferredoxin-like protein gene and the tetratricopeptide-repeat (TPR) protein gene. The two genes were selected for downstream investigation. Two SNP variations were observed in the exon for both genes, with one SNP resulting in changes in amino acid sequence. qPCR-based gene expression showed that both genes were highly expressed in the high-yielding double haploid lines along with the parental cultivar Westonia. In contrast, their expression was significantly lower in the low-yielding lines in the other parent. It can be concluded that these two genes are the contributing genes to the grain yield QTL.