Evaluating the shear fatigue behaviour of the interface between two bituminous layers is important in order to assure the structure can withstand repeated loadings. The 2T3C (Torsion-Traction-Compression sur Cylindre Creux, in French) hollow cylinder apparatus developed at the University of Lyon/ENTPE can apply torsion, tension, and compression on hollow cylinder samples. In this study, the apparatus is combined with 3D Digital Image Correlation (3D-DIC) to study the fatigue behaviour of the double-layered hollow cylinder samples with the interfaces with a tack coat made of emulsified bitumen. The fatigue failure was observed at the interfaces and not in layers. Six fatigue criteria are proposed to determine the fatigue life of an interface. They are: (1) the 50% reduction of stiffness criterion; (2) the maximal phase angle criterion, (3) maximal dissipated energy criterion, (4) slope change in the dissipated energy evolution curve criterion, (5) slope change in the stress evolution curve criterion, and (6) slope change in the displacement gap at the interface evolution curve criterion. The first criterion is more conservative in comparison with the last five criteria.