Doses Of Essential Oil Research Articles

Improving the insecticidal efficacy of Thuja orientalis essential oil by gamma radiation against Trogodermagranarium

The essential oils isolated by hydrodistillation from Thuja orientalis unripe fruits exposed to gamma radiation at dose levels of 0, 1, 3, and 5 kGy were analyzed by GC–mass spectrometry (MS) and assessed for their insecticidal activities on Trogoderma granarium Everts. The results showed slight changes were observed in the chemical composition of essential oil. The results showed that irradiated oil treatments induced higher toxicity to larvae of T. granarium than unirradiated oil with LD50 values (41.5, 54.2, and 87.1 μl/larva), and LD90 values (122.8, 207.5, and 314.7 μl/larva) for irradiated oil with 5.0, 3.0, and 1.0 kGy, respectively compared with unirradiated oil (LD50 = 153.8 μl/larva) and (LD90 = 622.8 μl/larva) by contact method. The same trend was obtained in the fumigant assay, the irradiated oil treatments with 3.0 and 5.0 kGy increased the toxicity to larvae of T. granarium than unirradiated oil with LC50 values were 280.7 and 183.6 μl/L, and LC90 values were 1171.6 and 687.4 μl/L, respectively compared with unirradiated oil (LC50 = 449.4 μl/L) and (LC90 = 1656.1 μl/L). The results showed also, that at 0.063 and 0.126 μl/cm2, the irradiated T. orientalis with 5.0 kGy, showed strong repellent activity against T. granarium larvae with the percentage repellency 100% (Class V) compared with the unirradiated oil (23.3 and 53.3%) after 2 h treatment. While at 0.252 μl/cm2, all three irradiated T. orientalis essential oil doses caused complete repellence for T. granarium larvae compared with the unirradiated oil (90%) after 2 h treatment. The present findings indicated that gamma radiation improves the insecticidal efficacy of T. orientalis essential oil and could be successfully used for the management of this insect pest.

Antimicrobial efficiency of rosemary, thyme and clove essential oils on the preservation of marinated chicken breasts (fillets)

Essential oils can be used as natural preservatives in the poultry meat industry. The aim of this research was to determine the effect of some essential oils on the microbial, physicochemical, and sensory properties of marinated chicken breast. For this purpose, rosemary, thyme, and clove essential oils were used at doses of 125 mg·kg-1 and 250 mg·kg-1 while marinating chicken breasts. After the marinated chicken breasts were divided into groups, they were stored in the refrigerator at 4°C. The results showed that 250 mg·kg-1 doses of essential oils, especially at 24 h, and rosemary had more inhibitory effects on some microbial (total mesophilic aerobic, total psychrophilic aerobic, and yeast–mold) parameters. However, the sensory groups with the addition of 125 mg·kg-1 were more accepted. Among these groups, the most acceptable group was the group that added 125 mg·kg-1 of thyme essential oil. The study shows that the use of thyme essential oil as an alternative to chemical preservatives may be beneficial both in extending the shelf life of marinated chicken breasts and in terms of consumer taste.

Origanum dubium Boiss. (Cypriot oregano) Use for the Preservation of Fresh Spearmint Quality and Safety

Very little is known about the preservation and storage of fresh medicinal and aromatic plants (MAPs) and/or herbs. As with many leafy vegetables, i.e., lettuce, fresh spearmint is a very perishable product throughout the production line and requires special handling/processing. The current study aimed to examine the antibacterial activity of selected MAPs (Origanum dubium, Salvia fruticosa and Sideritis cypria) grown in Cyprus towards foodborne pathogens. Then, the most effective MAPs’ essential oil (EO) and hydrosol (at different doses; combination of concentration and time of dipping submerge) were tested to preserve fresh spearmint quality and safety. The results showed that O. dubium EO and hydrosol presented great in vitro antibacterial activity against four foodborne pathogens and those products were further selected for application on fresh spearmint. During sensory evaluation, it was observed that higher O. dubium EO concentrations and longer time of application resulted into a less marketable product (less acceptable with less spearmint-like aroma), while hydrosol resulted in a more marketable product even at high doses. In general, EO and hydrosol applications increased spearmint’s antioxidants (including polyphenols, flavonoids, and ascorbic acid), resulting in a product of increased nutritional value. The bacterial populations of Salmonella enterica and Listeria monocytogenes inoculated on fresh spearmint were decreased with the EO and hydrosol application doses applied, and their effects were evident even after six days of storage at 4 °C. From the findings of this study, it can be concluded that O. dubium EO and hydrosol could be a potential sanitation method for fresh spearmint preservation.

Comparative assessment of anti-ulcer effects: Hydro-ethanolic extract from air-dried ray and disc florets vs essential oil of traditional Tagetes erecta (Asteraceae) in Swiss Albino rats

The Tagetes erecta Linn (family: Asteraceae) herb possesses medicinal and antioxidants value. The herb is traditionally used to treat various ailments, including ulcers. In order to investigate antiulcer activity of T. erecta herb, experimental study was performed between ray and disc florets extracted with 30 parts of water and 70 parts of ethanol solvents using Soxhlet apparatus and leaf's essential oil extracted in Clevenger apparatus by steam distillation method. Two doses of the hydroethanolic extract (400 and 800 mg/kg, Per os) and single dose of essential oil (10 ml/Kg, Per os) were administered for five consecutive days. Rat's pyloric ligation and aspirin (200 mg/kg, Per os) were used to induce acute and chronic gastric ulcers respectively. Antiulcer activity of both the herb's parts was compared on gastric juice parameters (pH, free acidity, total acidity) ulcer-index and ulcer percent protection. The p value of less than 0.05 was considered significant in statistical analysis. Elevation in pH, reduction in free acidity, total acidity and ulcer-index in both the ulcer models confirm that the test drug showed the ulcer-protective activity in rats. Dose-dependent effects were observed in the antiulcer activity of the hydroethanolic extract. However, the leaf's essential oil showed 94 % and 96.93 % ulcer protection in the pyloric ligation and aspirin-induced gastric ulcer models respectively. The hydroethanolic extract and the essential oil may be capable in part, regulating gastric acid secretion pathways or stimulating the cytoprotective secretion of bicarbonate, mucus and prostaglandins. Histological studies supported the observed antiulcer activity of the herb. The essential oil has more potent and significant anti-ulcer activity than hydro-ethanolic extract and respective standard drugs: ranitidine (50 mg/kg, Per os) & pantoprazole (20 mg/kg, Per os). The herb exhibits antiulcer activity, which could be due to the presence of lead phytochemicals: Quercetin-3-methyl ether, quercetin-7-methyl-ether & kaempferol flavonoids in the extract and limonene & terpinolene in leaf's essential oil and, thus, confirmed the traditional uses of T. erecta in the treatment of ulcers. The herb may cure acid pepsin disorders.

Oregano Essential Oil as a Natural Plant Additive Affects Growth Performance and Serum Antibody Levels by Regulating the Rumen Microbiota of Calves.

This experiment aimed to investigate whether supplementation of calves with different doses of oregano essential oil (OEO) could promote the development of the gastrointestinal tract and enhance the immune ability of calves by regulating the rumen microbiota. Twenty-four 70-day-old healthy and disease-free Holstein male calves were randomly divided into four groups, with the control group fed a basal diet, and the treatment group provided 4 g, 6 g, and 8 g of oregano essential oil per day in addition to the basal diet. After the 14-day pre-test, a 56-day formal test was conducted. At days 0 and 56 of the standard test period, calves were weighed, the average daily weight gain of calves during the test period was calculated, and serum samples were collected to measure the concentration of immunoglobulins (IgA, IgG, and IgM) in the serum; at day 56 of the formal test period, rumen fluid was collected from the calves, and 16SrRNA was sequenced to analyze changes in the rumen microbiota of the calves. The changes in the rumen microbiota of calves were analyzed by 16SrRNA sequencing. The results of the study showed that (1) OEO supplementation in calves significantly increased end weight and average daily gain (p < 0.05); (2) OEO supplementation in calves significantly increased serum concentrations of immunoglobulins IgA and IgM (p < 0.05); (3) OEO supplementation in calves significantly increased the abundance and diversity of rumen microbial organisms (p < 0.05); (4) OEO supplementation in calves significantly regulates the relative abundance of some species, and biomarkers with significant differences were screened by LEfSe analysis: g_Turicibacter, g_Romboutsia, f_Peptostreptococcaceae, f_Clostridiaceae, g_Clostridium_sensu_stricto_1, o_Clostridiales, g_unclassified_f_Synergistaceae, c_Coriobacteriia, o_Coriobacteriales, f_Atopobiaceae, g_Olsenella, p_Actinobacteriota, g_Defluviitaleaceae_UCG-011, f_Defluviitaleaceae, o_Corynebacteriales, g_Corynebacterium, f_Corynebacteriaceae, g_Shuttleworthia, f_Hungateiclostridiaceae, o_norank_c_Clostridia, g_Saccharofermentans, g_Streptococcus, f_Streptococcaceae, g_unclassified_o_Oscillospirales, and f_unclassified_o_Oscillospirales (p < 0.05, LDA ≥ 3); and (5) OEO supplementation in calves significantly enriched the metabolism of cofactors and vitamins pathway (p < 0.05). (6) Using Superman's correlation analysis, we screened unclassified_c_Clostridia, Shuttleworthia, and Christensenellaceae_R-7_group, three beneficial strains for calves. (7) Daily supplementation with 8g of OEO significantly affected rumen microbiota regulation in calves.

Bazı Uçucu Yağların Kimyasal Yapısı ve Laboratuvar Koşullarında Ceratitis capitata ‘nın (Wiedemann) (Diptera: Tephritidae) Larva Canlılığı Üzerindeki İnsekitisidal Etkisi

Aims:The Mediterranean fruit fly, medfly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) is a serious pest of many fruits and vegetables. This study was conducted to determine antifeeding and insecticidal activities of some essential oils extracted from Pelargonium graveolens (Geraniaceae), geranium, Lavandula intermedia Mill. (Lamiaceae), lavender, Nigella sativa L. (Ranunculaceae) black cumin, and Laurus nobilis L. (Lauraceae), laurel, against second instars of the Medfly on artificial diet.&#x0D; Material and Methods:The essential oils were obtained by Clevenger-type water distillation and a laboratory reared medfly colony was used in the study. Each tested essential oils with 6 different doses were distributed over the artificial diet in a Petri dish having 20 larvae. All experiments were performed under laboratory conditions of 23±1°C, 50% RH and 16: 8 (L:D) photoperiods. Probit MsChart was used to estimate LC50 and LC90 values of the tested essential oils. GGE Biplot analyzes were created with the larval mortality based on the different essential oil doses.&#x0D; &#x0D; Results: As results, the highest larval mortality was determined with addition of laurel and black cumin oils into the diet. The highest mortality was detected in black cumin oil at the lowest concentrations. Based on GGE Biplot analyzes, the essential oil of black cumin has observable larvicidal properties. &#x0D; Discussion: The results were provided fundemental information about insecticidal and antifeeding properties of the medfly in laboratory.&#x0D; Conclusions:Further studies needed to integrate sustainable management programmes with natural insecticides against the medfly larvae.

Immunity, rumen metagenomics, ruminal variables, and growth performance of calves fed milk with sage (Salvia officinalis) essential oil.

The study aimed to determine the effect of sage (Salvia officinalis) essential oil (EO) to the drinking milk until the weaning stage of Holstein calves on the growth performance, body measurements, ruminal fermentation, rumen metagenomic profile, proinflammatory cytokines [interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and IL-1β], immune globulins (IgG, IgM, and IgE), and acute phase proteins (serum amyloid-A, SAA). In the study, 24 Holstein calves were divided into three groups as 0 μL (SAG0; control group), 100 μL (SAG100), or 200 μL (SAG200) of sage EO to the milk per calf per day. The addition of sage EO to the milk linearly increased the live weight, feed intake, and daily body weight gain (P < 0.05). The addition of sage EO to the milk in calves linearly increased serum IgG titter dose dependently (P < 0.05), but serum IgM and IgE titters did not change (P > 0.05). The concentrations of serum TNF-α, IL-1β, and IL-6 in the weaned calves linearly decreased by the sage aromatic oil addition to the milk (P < 0.05). The serum SAA concentrations of calves did not differ among the control and treatment groups (P > 0.05). Probiotic Bifidobacterium and Acidaminococcus genusin calf rumen fluid canincrease bysage EO addition to milk. The relative abundance of genus Prevotella, Prevotellaceae_NK3B31_group, and Prevotella_9 increase with sage EO. The ruminal ammonia-nitrogen (NH3-N) concentration and total short chain fatty acid (T-SCFA) molarity decreased by sage EO addition to the drinking milk (P < 0.05). Themolarities of iso butyric (IBA) and iso valeric acids (IVA) in rumen fluid of the weaned calves linearly reduced by the increasing sage EO dose to the milk (P < 0.05). The butyric acid (BA) and iso caproic acid (ICA) molarities in rumen fluid did not change by the sage EO addition (P > 0.05). Consequently, it has been observed that sage EO addition to the milk of calves positively affected the immune system variables and performance parameters.Sage EO additionof calves before weaning may support the immune system in the eventual immunosuppression at the weaning stage.

Callosobruchus maculatus males and females respond differently to grandparental effects.

In this study, we used the cowpea weevil Callosobruchus maculatus (Coleoptera: Chrysomelidae) and two essential oils (mint and rosemary) to investigate the effect of the parents (F0) exposure to a sublethal dose of essential oil on grand offspring (F2) encountering the same essential oil. Then we evaluated biological parameters, including immature development time, sex ratio, adult emergence, egg number, egg hatch, longevity, and mating behaviors in three generations (F0, F1, and F2). Results showed when F0 experienced essential oil in the embryonic stage, parental and grandparental effects were more severe than adulthood experiences. Also, grandparental effects increased or decreased reactions of F2 generation when faced with a similar essential oil, depending on grand offspring sex. For example, when grandparents experienced rosemary essential oil in the embryonic stage, they produced more tolerant female grand offspring with a better ability to cope with the same essential oil (increased adult longevity and egg number). However, male grandoffspring were more sensitive (had a higher mortality percentage and less copulation success). Grandparental effects of exposure to mint essential oil diminished female grand offspring longevity and improved male copulation behavior parameters such as increased copulation duration and decreased rejection by females. In all, grandparental effects were different in male and female grand offspring based on the essential oil type experienced by F0.

Chemical profiling and assessment of biological activities of wild Artemisia absinthium L. essential oil from Algeria

Artemisia absinthium L. (A. absinthium L.) is an important perennial shrubby plant widely employed in traditional medicine for the treatment of several ailments including gastritis, wound healing, and digestive disorders. In this study we assessed the chemical composition, as well as the antioxidant, anti-inflammatory and analgesic activities of a pure essential oil obtained from the aerial part of A. absinthium L., growing wild in Cherchell (Tipaza, Algeria). The essential oil, obtained through hydrodistillation using a Clevenger-type apparatus, underwent gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS) analysis. The essential oil yield was 0.67 ± 0.02% (w/w), comprising 38 identified components, constituting 89.05% of the essential oil. Notable components included trans-thujone (54.7%), chamazulene (10.05%), β-eudesmol (4.7%), cis-thujone (4.2%), terpinen-4-ol (4.2%), and germacrene D (4.1%). Antioxidant activities were assessed via the DPPH٠free radical test (IC50: 489.19 ± 5.28 μg/mL) and the reducing power test (EC0.5: 424.95 ± 1.25 μg/mL). While the essential oil exhibited relatively low antioxidant activity compared to standard antioxidants, it showed significant antioxidant activity in the β-carotene-linoleic acid test (78.38 ± 0.83%). An acute toxicity study using various essential oil doses (10-100 mg/kg) revealed no signs of toxicity or mortality. Anti-inflammatory and antinociceptive activities were observed when testing the essential oil on carrageenan-induced paw edema in mice and acetic acid-induced pain in mice, with significant reductions (P < 0.05). In conclusion, A. absinthium L. essential oil possesses antioxidant, anti-inflammatory, and antinociceptive properties, suggesting its potential as a natural preservative ingredient in the food, cosmetics, and pharmaceutical industries.

Effect of cinnamon essential oil dietary supplementation on the growth, fatty acid composition, and meat quality of tilapia.

This study was conducted to investigate the effects of dietary supplementation of 0%, 0.2%, 0.35%, and 0.5% cinnamon essential oil on growth performance, fatty acid, and fillet quality of tilapia (Oreochromis niloticus). The results of growth experiments showed that the weight gain rate linearly and quadratically increased with increasing cinnamon essential oil doses (p<0.05). The results of fatty acids experiments showed that the addition of essential oil significantly decreased the saturated fatty acids levels from 36.67% to 30.82% and increased the polyunsaturated fatty acids (PUFA) levels from 24.55% to 46.89%; especially, the n-3 PUFA of 0.5% essential oil treatment showed the highest levels. Moreover, the n-6 PUFA of 0.2% essential oil treatment were increased from 22.17% to 32.99%. The results of fillet quality experiments showed that the hardness and cohesiveness were linearly and quadratically increased with the increasing essential oil doses on days 4 and 7, respectively. The b* values linearly and quadratically decreased as the doses increased on day 7 (p<0.05). The total volatile basic nitrogen levels were quadratically decreased with increasing cinnamon essential oil doses on day 7 (p<0.05). In general, it can be concluded that cinnamon essential oil presented positive effects on the growth, nutritive values, and meat quality in tilapia.

The Potential of Essential Oils from Active Packaging to Reduce Ethylene Biosynthesis in Plant Products. Part 2: Fruits (Blueberries and Blackberries).

Plant essential oils (EOs) have an important ability to inhibit ethylene biosynthesis. Nevertheless, the effects of EOs on the key components of ethylene biosynthesis (l-aminocyclopropane-1-carboxylic (ACC) oxidase activity, ACC synthase activity, and ACC content) have not yet been thoroughly studied. Accordingly, this study focused on the effects of emitted EOs from active packaging (EO doses from 100 to 1000 mg m-2) on the key components of ethylene biosynthesis of blueberries and blackberries under several storage temperatures. Anise EO and lemon EO active packaging induced the greatest inhibitory effects (60-76%) on the ethylene production of blueberries and blackberries, respectively, even at high storage temperatures (22 °C). In terms of EO doses, active packaging with 1000 mg m-2 of anise EO or lemon EO led to the highest reduction of ethylene production, respectively. At 22 °C, the investigated EO active packing reduced the activities of ACC synthase and ACC oxidase up to 50%. In order to minimise ethylene biosynthesis in blueberries and blackberries when they are stored even under improper temperature scenarios at high temperatures, this EO active packaging is a natural and efficient technological solution.

The Potential of Essential Oils from Active Packaging to Reduce Ethylene Biosynthesis in Plant Products. Part 1: Vegetables (Broccoli and Tomato).

Essential oils (EOs) extracted from plants have a high potential to reduce ethylene biosynthesis, although their effects have not been deeply studied yet on the key components of the ethylene biosynthesis pathway: l-aminocyclopropane-1-carboxylic (ACC) oxidase activity, ACC synthase activity, and ACC content. Hence, the present study aimed to elucidate the effects of released EOs from active packaging (with different EO doses ranging from 100 to 1000 mg m-2) on the ethylene biosynthesis key components of broccoli and tomato under different storage temperature scenarios. The largest ethylene inhibitory effects on broccoli and tomatoes were demonstrated by grapefruit EO and thyme essential EO (up to 63%), respectively, which were more pronounced at higher temperatures. Regarding EO doses, active packaging with a thyme EO dose of 1000 mg m-2 resulted in the strongest reduction (33-38%) of ethylene production in tomatoes. For broccoli, identical results were shown with a lower grapefruit EO dose of 500 mg m-2. The studied EO-active packaging decreased ACC synthase and ACC oxidase activities by 40-50% at 22 °C. Therefore, this EO-active packaging is a natural and effective technology to reduce ethylene biosynthesis in broccoli and tomatoes when they are stored, even in unsuitable scenarios at high temperatures.

Chemical composition and herbicidal activity of Oregano (Origanum onites) essential oil on weeds and wheat

ABSTRACT Oregano essential oil (EO) is known as one of the EOs with the highest biological activity on target plant species. In this study, the inhibitory effects of different doses of oregano (Origanum onites L.) EO on the germination and seedling growth of some wheat cultivars and some problem weed species in wheat fields was investigated. For this purpose, both laboratory and pot experiments were carried out. Oregano EO obtained by steam distillation method and component analysis was made via GS/MS. Germination tests and pot experiments were carried out under controlled laboratory and greenhouse conditions. In the study, five wheat varieties, four for bread and one for durum, and five different weed species were used. The effects of different doses of oregano EO on the germination rate, viable plant rate, seedling length, and biomass of test plants were analyzed. According to the results, it was observed that oregano EO showed bioactivity on all species and cultivars included in the experiment. In terms of all parameters, it was concluded that wheat varieties showed higher resistance to oregano EO than weed species. These results show that oregano EO can be used to control some weed species that are a problem in wheat.

A Study of the Chemical Composition, Acute and Subacute Toxicity of Bulgarian Tanacetum parthenium Essential Oil.

Tanacetum parthenium (L.) Sch.Bip. (T. parthenium) is an aromatic perennial plant belonging to the Asteraceae family, also known as feverfew. It is widely distributed in various regions of Europe and other parts of the world. The plant has a rich background in the traditional medicine of many nations and has been used as a remedy for fever, pain, inflammation, asthma, rheumatism, menstrual disorders, etc. Methods: GC-MS analysis was conducted to determine the chemical composition of the isolated essential oil (EO). Using the method proposed by Litchfield and Wilcoxon, the average lethal dose (LD50) of the EO on Wistar rats was determined for two routes of administration: oral (p.o.) and intraperitoneal (i.p.). The subacute toxicity of the EO was also tested by oral administration of a daily dose of 1.0 g/kg body weight (BW) for 28 days. The toxicity of the EO was evaluated by observing and evaluating changes in behavior, body weight, basic hematological and serum biochemical parameters, and histopathological changes of the internal organs. Thirty-seven volatile organic compounds representing 94.58% of the total oil composition were tentatively detected in the obtained T. parthenium EO. The dominant compounds were camphor (45.47%), trans-chrisantenyl acetate (21.65%), camphene (9.48%), and cis-isogeraniol (5.42%). The results showed that the EO was not toxic when administered in acute oral doses. The acute mean lethal dose for intraperitoneal administration was LD50 i.p. = 2.13 g/kg BW. In the subacute study involving administration of an oral dose of EO for 28 days, there were a number of changes in the hematological and serum biochemical parameters of the blood compared with the control group of animals. However, no symptoms of toxicity, changes in the body weight of the rats, death, or pathological changes in the histological indicators of the examined organs-brain, heart, stomach, liver, spleen and kidney-were found. Extrapolating the results obtained from the rat experiments, we can state that the EO is safe for use in doses below 1 g/kgBW for a period not exceeding one month.

Lower dose of plant substance more effective in repelling Rhyzopertha dominica F . (Coleoptera, Bostrichidae) and Sitophilus granarius L. (Coleoptera, Dryophthoridae).

The study compares the effect of two concentrations (0.1% and 1%) of plant material on repelling two dangerous stored pests - Rhyzopertha dominica F. and Sitophilus granarius L. Both species were treated with the essential oils of Foeniculum vulgare Mill., Carum carvi L., Mentha pulegium L., and Cananga odorata (Lam.) Hook.f. & Thomson, as well as the chemicals contained in these oils: anethole, L-carvone, pulegone and thymoquinone. Laboratory tests were carried out at 29±1 °C with 60±5% relative humidity (RH). Repellence of insects were noted after 1, 2, 3, 4, 5, 24 and 48 h. A lower dose of essential oils and some of the tested compounds caused a stronger repellence in the specified species of beetles. In the case of R. dominica, the concentration of 0.1% pulegone and L-carvone had the greatest repellent effect. Differences in the repellence of R. dominica were found between the concentrations of 0.1%-1% among all the tested substances in each time interval. This difference ranged from 5.0%-52.92%. All substances used in the tests had a strong repellent effect on S. granarius (30%-100%). There were differences in the repellency of S. granarius between 0.1%-1% concentration in most of the tested substances in each time interval, but they were not as large as for R. dominica.. The most interesting results were obtained concerning the relationship: the lower tested substance concentration caused a much stronger repellent effect of R. dominica and S. granarius..

Elucidating the role of two types of essential oils in regulating antibiotic resistance in soil

Although several approaches for reducing antibiotic resistance genes (ARGs) in soil have been proposed, the application of environmentally friendly approaches is now attracting much more attention. In the present study, two types of essential oils (EOs), namely lavender essential oil (LEO) and oregano essential oil (OEO), were selected to investigate their roles in regulating ARGs in soil. In a 28-day microcosm experiment, it was found that the different types and doses of EOs significantly changed the composition of microbial communities. The LEO treatments enriched more taxa belonging to Actinobacteria than the control, whereas the low dose of OEO reduced Actinobacteria enrichment. Besides, the control and the treatments with a high dose of LEO and OEO all significantly enriched the functional pathways related to Human Diseases, which were positively associated with ARGs. However, the low dose of these EOs helped to reduce the pathways. Because of inhibition of the functional pathways and ARG hosts, the low dose of OEO reduce the ARGs related to antibiotic efflux by 71.8% and the resistance genes to multidrug by 56.4%, but these roles did not occur in LEO treatments. These outcomes provide practical and theoretical support for the application of EOs in remediating ARG-contaminated soils.

Impact of dietary supplementation with Cinnamomum cassia essential oil on silver catfish (Rhamdia quelen): Toxicological effects and muscle antioxidant and fatty acid profiles

ABSTRACT The aim of this study was to determine the biological effects of dietary supplementation with 0.05% and 0.1% cinnamon essential oil extracted from Cinnamomum cassia on silver catfish (Rhamdia quelen). The final body weight, weight gain, and specific growth rate were significantly higher in fish supplemented with 0.05% cinnamon essential oil than in the control(untreated) group. Muscle reactive oxygen species and lipid peroxidation levels were significantly lower in fish supplemented with 0.05% cinnamon essential oil but higher at the 0.1% concentration. Muscle antioxidant capacity against peroxyl radicals (ACAP) and superoxide dismutase activity were significantly higher in fish supplemented with 0.05% cinnamon essential oil, while ACAP levels were lower in fish supplemented with 0.1%. The total saturated fatty acid content was significantly higher in the muscle of supplemented fish than in controls, while the total monounsaturated fatty acid content was significantly higher only in fish fed 0.1% cinnamon essential oil. Finally, the total content of polyunsaturated fatty acids was significantly lower in fish fed 0.1% essential oil. Thus, data demonstrated that 0.05% C. cassia essential oil improves fish health by improving performance and muscle oxidant/antioxidant status. Higher doses of cinnamon essential oil produced oxidative stress in muscle, suggesting toxicity at the 0.1% level. Although this cinnamon essential oil diet exerted positive health effects, this diet impaired the muscle fatty acid profile, suggesting adverse impacts on human health.

Seed priming with essential oils for sustainable wheat agriculture in semi-arid region.

Drought is one of the major constraints to global crop production. A number of sustainable systems have focused on the development of environmentally friendly innovative biotechnological interventions to prevent yield losses. The use of essential oils as a seed priming agent can make an important contribution as a natural stimulant in increasing drought stress tolerance. This study focuses on the effects of seeds coated with different doses (D0 (0%), D1 (0.01%), D2 (0.05%), D3 (0.10%) and D4 (0.25%)) of sage, rosemary and lavender essential oils on wheat germination, seedling establishment and yield parameters. Turkey's local wheat genotype Köse was used as plant material. The impact of the seed priming on germination rate, coleoptile length, shoot length, root length, shoot fresh and dry weight, root fresh and dry weight, relative water content (RWC), proline, and chlorophyll contents was assessed in laboratory experiments. In addition, the effect of essential oil types on yield parameters and agronomic components (plant height, spike height, number of grains per spike, grain yield per spike, grain yield per unit area, thousand-grain weight) was evaluated in a field experiment during the 2019-2020 crop seasons in a semi-arid climate. According to laboratory results, the highest germination rate among all treatment doses was determined in the D2 treatment (rosemary 93.30%, sage 94.00% and lavender 92.50%), while the lowest germination rates for all essential oil types were determined in the D4 treatment (rosemary 41.70%, sage 40.90% and lavender 40.90%). Increasing treatment doses showed a similar suppressive effect on the other parameters. In the field experiment, the highest grain yield (256.52 kg/da) and thousand-grain weight (43.30 g) were determined in the rosemary treatment. However, the priming treatment has an insignificant on the number of grains per spike and the spike length. The light of these results, the effects of essential oil types and doses on yield parameters were discussed. The findings highlight the importance of using essential oils in seed priming methods for sustainable agricultural practices.

In vitro Antifungal Effects of Plant Essential Oils Against Some Fungal Disease Agents Causing Internal Rot of Dried Pepper Fruits

Hasat öncesi ve sonrası tarımsal ürünlerde meyve iç çürüklüğüne neden olan fungal etmenler ciddi kalite ve verim kayıplarına neden olurken, gıdalarda ürettikleri mikotoksinler tüketiciler için sağlık riski oluşturur. Bu çalışmada, Hatay ilinde yetiştirilen ve yerel pazarlarda satılan kurutmalık biber meyvelerinde iç çürüklüğü belirtisine neden olan fungal hastalık etmenlerinin, izolasyonu, morfolojik ve MALDI-TOF yöntemleri ile tanılanması, yaygın hastalık etmenlerine karşı farklı bitki uçucu yağlarının antifungal etkilerinin belirlenmesi amaçlanmıştır. Hastalık belirtisi gösteren meyve örneklerinden yapılan izolasyonları müteakiben yapılan morfolojik ve MALDI-TOF tanılama çalışmaları sonucunda Alternaria alternata ve Aspergillus niger en sık rastlanan fungal hastalık etmenleri olarak belirlenmiştir. Hastalık etmenlerine karşı kekik (Thymus vulgaris L., Tymbra spicata L. ve Origanum syriacum L.), rezene (Foeniculum vulgare Mill.), defne (Laurus nobilis L.) ve okaliptüs (Eucalyptus camaldulensis Dehnh) uçucu yağlarının buhar fazında farklı dozlarının antifungal etkinlikleri in vitro koşullarda araştırılmıştır. Fungus izolatlarına karşı en yüksek antifungal etkinlik (% 100 engelleme) Thymbra spicata, Origanum syriacum ve Thymus vulgaris uçucu yağlarının 4.0-6.0 µl petri-1 dozlarında gözlenmiştir. Test edilen fungal izolatlara karşı en düşük antifungal etkinlik ise Eucalyptus camaldulensis (16.0-40.0 µl petri-1) uçucu yağı tarafından gösterilmiştir. Yapılan çalışmalarla uçucu yağların antifungal özellikleri ve EC50 değerleri belirlenmiştir. Elde edilen sonuçlara göre bitki uçucu yağları, doza bağlı bir şekilde test edilen izolatlara karşı antifungal etkinlik göstermişlerdir. Elde edilen sonuçlar bitki uçucu yağlarının, kurutmalık tarımsal ürünlerde biyofumigant olarak uygulanabileceğini göstermiştir.

A Status Review on Health-Promoting Properties and Global Regulation of Essential Oils.

Since ancient times, essential oils (EOs) have been known for their therapeutic potential against many health issues. Recent studies suggest that EOs may contribute to the regulation and modulation of various biomarkers and cellular pathways responsible for metabolic health as well as the development of many diseases, including cancer, obesity, diabetes, cardiovascular diseases, and bacterial infections. During metabolic dysfunction and even infections, the immune system becomes compromised and releases pro-inflammatory cytokines that lead to serious health consequences. The bioactive compounds present in EOs (especially terpenoids and phenylpropanoids) with different chemical compositions from fruits, vegetables, and medicinal plants confer protection against these metabolic and infectious diseases through anti-inflammatory, antioxidant, anti-cancer, and anti-microbial properties. In this review, we have highlighted some targeted physiological and cellular actions through which EOs may exhibit anti-inflammatory, anti-cancer, and anti-microbial properties. In addition, it has been observed that EOs from specific plant sources may play a significant role in the prevention of obesity, diabetes, hypertension, dyslipidemia, microbial infections, and increasing breast milk production, along with improvements in heart, liver, and brain health. The current status of the bioactive activities of EOs and their therapeutic effects are covered in this review. However, with respect to the health benefits of EOs, it is very important to regulate the dose and usage of EOs to reduce their adverse health effects. Therefore, we specified that some countries have their own regulatory bodies while others follow WHO and FAO standards and legislation for the use of EOs.