Dose Of Diminazene Aceturate Research Articles

Disposition of diminazene aceturate (Berenil ®) in trypanosome-infected pregnant and lactating cows

Three cows were repeatedly infected with different strains of Trypanosoma congolense and treated intramuscularly each time with a different dose of diminazene aceturate (Berenil ®). Biphasic decline was observed of the maximal plasma drug levels, which were attained at 15 min after the first treatment and at 30 min after the second and third treatments. The rate constants for the distribution and terminal phases depended on the period of exposure to parasitaemia of the animal at the time of treatment. Maximal diminazene aceturate residue levels were found in milk 8 h post treatment and declined biexponentially to 4.56 ng ml −1 and 8.76 ng ml −1 at 21 days post treatment after 3.5 mg kg −1 and 7.0 mg kg −1 doses, respectively. In the three cows, higher drug residues were found in the kidney (7.04, 3.92 and 7.99 μg g −1) than in liver (3.26, 2.87 and 1.24 μg g −1) and heart (1.79, 1.25 and 1.03 μg g −1). The results of this study indicate that the level of parasitaemia (degree of anaemia) in the animal at the time of treatment affects the distribution, disposition and elimination of diminazene aceturate in the animal. Furthermore, the residue level in milk after treatment depends on the treatment dose and could easily be bioavailable to the consumer.

Chemoprophylaxis with diminazene aceturate in experimental Babesia bigemina infection in cattle

Four-month-old fully susceptible cross bred calves were immunised against Babesia bigemina infection by treating them, during the prepatent period, with one or two doses of diminazene aceturate at 3 mg (kg bodyweight)-1. After 45 days the recovered calves were challenged with the homologous strain of B bigemina infected blood. The ensuing host responses, body temperature, parasitaemia, haemoglobin, packed cell volume, haemoglobinuria, capillary agglutinating antibodies and mortality, were studied. The four untreated control calves developed a severe form of the disease with classical symptoms and two died. Calves of both groups which recovered resisted the challenge infection with a mild relapse in the immunised group, thus indicating a state of premunition. The effect of one dose of diminazene was sufficient to suppress all the clinical effects of the disease.