Doses Of UV Radiation Research Articles

Effects of photoacid generator decomposition on dissolution kinetics of poly(4-hydroxystyrene) in tetraalkylammonium hydroxide aqueous solutions

For the advancement of lithography, the resist materials and processes are the most critical factors. In particular, the process of developing resist materials is important in reducing the line width roughness and stochastic defects. In this study, a quartz crystal microbalance method was used to investigate the dissolution dynamics of poly(4-hydroxystyrene) (PHS) films containing triphenylsulfonium nonaflate (TPS-nf) in tetraalkylammonium hydroxide (TAAH) aqueous solutions. The time required for dissolution increased with the alkyl chain length of TAAH. The dissolution rate of PHS films increased with UV irradiation dose for tetramethylammonium hydroxide and tetraethylammonium hydroxide developers, whereas the dissolution time increased for the tetrabutylammonium hydroxide developer. For the tetrapropylammonium hydroxide developer, the dissolution time of PHS films without postexposure baking (PEB) slightly decreased with UV irradiation, whereas that with PEB increased. Both the surface free energy of PHS films and the molecular size of TAAH are considered to affect dissolution kinetics.

Long-Term Changes of Positive Anomalies of Erythema-Effective UV Irradiance Associated with Low Ozone Events in Germany 1983–2019

In order to assess whether there is an increasing need for adaptation to the associated human health risks, the long-term occurrence (1983–2019) of low-ozone events (LOEs) with associated near-surface anomalies of erythema-effective UV irradiance was examined using an impact-related approach. Based on satellite data, means of four locations in Germany (Sylt, Berlin, Frankfurt, Munich) were compared for three subperiods (T1: 1983–1989, T2: 1990–1997, T3: 1998–2019). The period of peak global ozone depletion in the 1990s (T2) is characterized by a larger frequency of LOEs than the preceding (T1) and the subsequent (T3) subperiods. During the most recent subperiod (T3), the mean number of LOEs is 1.1 ± 0.5 events/year, with a variability of 0 to 4.2 ± 0.8 events/year, and shows a statistically significant decrease in the annual number of −4.8%/year. The annual totals of the LOE-associated anomalies of the erythema-effective UV radiation dose show no trend during T3. With regard to LOE-associated UV index anomalies, spring is the season most affected by LOEs, with more than half of all cumulative UV peak loads, while the absolute maximum values of the LOE-associated UV index anomaly of about 1.8 UV index occur near the summer solstice. Within the most recent subperiod (T3), summer contributes an increasing share of the peak loads. Overall, the study confirms that LOEs pose health risks due to intermittent, pronounced positive anomalies in erythema-effective UV irradiance and therefore require special attention and adaptation measures. Long-term changes can be identified, but to date there has been no evidence of an increasing health risk in Germany.

Effect of germicidal short wavelength ultraviolet light on the polyphenols, vitamins, and microbial inactivation in a highly opaque apple juice

AbstractThe aim of this investigation was to study the efficacy of UV‐C light emitting diode (LED) system operating at 263 nm for the inactivation of Listeria monocytogenes and Escherichia coli O157:H7. UV‐C irradiation was applied to stirred 5 ml samples, using a collimated beam LED system operating at a 263 nm wavelength. UV irradiation doses ranging from 0 to 160 mJ/cm2 were also delivered to apple juice (AJ) and polyphenols and vitamins were profiled. Liquid chromatography–mass spectrometry (LC–MS/MS) analysis was conducted to assess the stability of polyphenols or vitamins in UV‐C exposed AJ. The polyphenol and vitamin results demonstrated that UV‐C irradiation in AJs at relevant commercial UV doses induced significant reductions in the concentrations of selected polyphenols and vitamins, p < .05. Ascorbic acid was reduced to 32%, at 160 mJ/cm2 whereas 17% reduction was observed at 40 mJ/cm2. Riboflavin was observed to be relatively stable. Epicatechin and chlorogenic were significantly reduced at high exposure doses. In contrast, minor changes were observed at 40 mJ/cm2. Results show that UV‐C irradiation effectively inactivated pathogenic microbes in AJ. Inactivation levels of all organisms were proportional to UV‐C dose. The log reduction kinetics of microorganisms followed log‐linear and with higher R2 (>0.95). The decimal reduction dose (D10) values of 4.16 and 3.84 mJ/cm2 were obtained from the inactivation of E. coli and L. monocytogenes in AJ. The results from this study imply that adequate log reduction of pathogens is achievable in AJ and suggests significant potential for UV‐C treatment of other opaque liquid foods.

Post-glass melting synthesis and photochromic properties of composite AgCl-AgPO3 glasses

We herein present a simple, fast, low-temperature, post-glass melting fabrication protocol in which a photochromic silver cation based modified zone is incorporated within silver metaphosphate glass (AgPO3). The selection of AgPO3 glass is mainly based on its relative “soft” nature (Tg = 192 °C) that enables the integration of silver cations from the surface deposited AgCl layer, while being transparent in most of the visible range, and therefore suitable for smart photochromic window applications. The suggested synthesis procedure permits the controlled formation of a silver cation modified layer within the host glass matrix, while the characteristics of the layer itself can be adjusted correspondingly. Our findings reveal a direct relationship between the developed composite AgCl–AgPO3 glass photochromic response and the morphological features of the integrated layer, i.e., thickness and position. More importantly, the photochromic response time with various UV irradiation doses is also studied, where remarkable response times of several seconds are obtained. Processes and efforts to further enhance the photochromic performance by utilizing the presence of silver nanoparticles within the glass matrix are also presented and discussed.

Techno-economic evaluation of UV light technologies in water remediation

Disinfection commonly follows conventional treatments in wastewater treatment and remediation plants aiming at reducing the presence of pathogens. However, the presence of the so called “micropollutants” has emerged as a serious concern, therefore developing tertiary treatments that are not only able to remove pathogens but also to degrade micropollutants is worth investigating. Nowadays, UV-C photo-degradation processes are widely used for disinfection due to their simplicity and easy operation; additionally, they have shown potential for the removal of contaminants of emerging concern. Conventional mercury lamps are being replaced by light-emitting diodes (LEDs) that avoid the use of toxic mercury and can be switched on and off with no effect on the lamp lifetime. This work aims to comparatively evaluate the performance of several photo-degradation technologies for the removal of two targeted micropollutants, the pharmaceutical dexamethasone (DXMT) and the herbicide S-metolachlor (MTLC), using UV irradiation doses typical of disinfection processes. To this end, the technical performance of UV-A/UV-C photolysis, UV-A/UV-C photocatalysis, UV-C/H2O2 and UV-C/NaOCl has been compared. The influence of operating conditions such as the initial concentration of the pollutants (3 mg L−1 - 30 mg L−1, concentrations found in membrane or adsorption remediation steps), pH (3−10), and water matrix (WWTP secondary effluent, and ultrapure water) on the degradation efficiency has been studied. The economic evaluation in terms of electricity and chemicals consumption and the carbon footprint has been evaluated. UV-C photolysis and UV-C photocatalysis appear as the most suitable technologies for the degradation of DXMT and MTLC, respectively, in terms of kinetics (1.53·10−1 min−1 for DXMT and 1.96·10−2 min−1 for MTLC), economic evaluation (1 € m−3 for DXMT and 32 € m−3 for MTLC) and environmental indicators (0.5 g-CO2 for DXMT and 223.1 g-CO2 for MTLC).

Investigation of the film-forming ability of proteins subjected to UV treatment for the dairy industry development

Film-forming mixtures based on reconstituted solutions of whey protein concentrates (WPC) with a protein content of 2.0 and 5.0% were subjected to UV irradiation in the range from 90 to 150 J/ml. With an increase in the dose of UV irradiation in film-forming solutions, the solubility of the protein decreased, the greatest effect was achieved in a mixture with a protein content of 2.0%. The decrease in solubility indirectly proved the process of aggregation of protein molecules in the studied solutions. It has been shown that UV exposure improves the strength properties of protein films. The highest specific stress at film rupture at an irradiation dose of 150 J/ml was 23.11 MPa in a 5.0% protein solution and 5.74 MPa in a 2.0% solution. The indicator of the applied force increased more intensively when processing a 5.0% protein solution, and amounted to 16.00 N in the case of a 2.0% solution, and 24.82 N in a 5.0% solution.

Removal of Microcystis aeruginosa and microcystin-LR by UV/Fenton system: Characteristics and degradation pathways

The increasing severity of cyanobacterial blooms owing to eutrophication in water bodies has caused widespread concern. In this study, the UV/Fenton system was evaluated for the removal efficiency of Microcystis aeruginosa, algal organic matter (AOM), and the intermediate degradation products of microcystin-LR (MC-LR) and their degradation pathways. Studies have shown that under the conditions of 300 μmol·L-1 H2O2, 125 μmol·L-1 FeSO4, 5 min UV irradiation (UV irradiation dose of 14.40 mJ·cm−2), and 10 min pre-oxidation, the removal rates of algae cells were 97.3 %, while the proteins with aromatic structures and soluble biological metabolites in the extracellular organic matter were significantly reduced, with low Fe and AOM residues reported at the end of sedimentation. Liquid chromatography-mass spectrometry analysis showed that HO produced in the UV/Fenton system can attack several reaction sites in the MC-LR molecule, including the aromatic ring and conjugated diene on the side chains of the Adda group, the CC bond of the Mdha group, and the amide bond on the polypeptide ring, and eventually mineralize to CO2, H2O, and inorganic ions. Therefore, the studied UV/Fenton system is an effective method for algae removal and MC-LR degradation, without secondary pollution.

Synthesis of ZnO@SiO2 core-shell structure-based thermal control coatings with enhanced UV irradiation stability

In recent years, core-shell structural materials have integrated the internal and external properties of materials, greatly improving the comprehensive properties of materials and attracting continuous attention in various fields. To develop an inorganic thermal control coating for surface radiators with low solar absorption and high spatial irradiation stability, this work prepared a core-shell structured pigment-based thermal control coating by sol-gel method and systematically investigated its optical properties as well as UV radiation stability. It was found that the optimal thickness of SiO2 in the shell layer was in the range of 17–77 nm, which had little effect on the optical properties of the core pigment. The solar absorption of the ZnO-based coating and the ZnO@SiO2-based coating were 0.14 and 0.15, respectively. In addition, the stability of ZnO@SiO2-based coatings is better than that of ZnO-based coatings under 1500 ESH UV irradiation dose.

The Effective Doses of Ultraviolet Irradiation of Eggs prior to Incubation by Amalgam Lamps

The disinfection of eggshells prior to the incubation of the eggs is an important element of biosecurity programs and pathogen elimination in poultry production. Ultraviolet (UV irradiation is an effective method of disinfection of eggs prior to incubation. Presently the lamps where free mercury is substituted with amalgam are increasingly used as the UV source and the application of these lamps for the disinfection of eggs is an actual topic of research. The study presented was aimed at the determination of the effective doses of UV irradiation by bactericide amalgam lamps for the inactivation of pathogenic microbial species contaminating the surface of the eggs. The preliminary cultivated test strains of the following pathogens were used: Salmonella paratyphi A №225, Escherichia coli АТСС 25922, Salmonella enteritidis №64, Proteus vulgaris HX 19 222, Staphylococcus aureus ATCC 6538-P. The eggs were directly irradiated by open-type bactericide irradiator “Svetolit 90ТN” (300 Wt; 87–90 Wt of bactericide UV radiation at wavelength 254 nm). It was found that the doses of the irradiation 82.5 mJ/cm2 with the distance between amalgam lamp and eggs 20 cm and 92.3 mJ/cm2 with the distance 35 cm effectively inactivated all test species (by 99.8–99.9%) while dose 62.1 mJ/cm2 with the distance 50 cm was less effective (inactivation efficiency 95.0–99.1%). At the doses 41.3 and 30.8 mJ/cm2 and the gaps between the amalgam lamp and eggs 20 and 35 cm the least resistant to UV-irradiation strain was Staphylococcus aureus ATCC 6538-P (elimination efficiency 90.0%). The inactivation of the strain Escherichia coli АТСС 25922 was more affected by the duration of the exposure to UV than by the gap between the lamp and the eggs.

Ultraviolet irradiation doses for coronavirus inactivation -review and analysis of coronavirus photo inactivation studies

Background: To slow the increasing global spread of the SARS-CoV-2 virus, appropriate disinfection techniques are required. Ultraviolet radianon (UV) has a well-known antiviral effect. But measurements on the radiation dose necessary to inactivate SARS-CoV-2 have not been pub- lished so far. Methods: Coronavirus inactivation experiments with ultraviolet light performed in the past were evaluated to determine the UV radiation dose required for a 90% virus reduction. This analysis is based on the fact that all coronaviruses have a similar structure and similar RNA strand length. Results: The available data reveals large variations, which are apparently not caused by the coronaviruses but by the experimental conditions selected. If these are excluded as far as possible, it appears that coronaviruses are very UV sensitive. The upper limit determined for the log-reduction dose (90% reduction) is approximately 10.6 mJ/cm2 (median), while the true value is probably only 3.7 mJ/cm2 (median). Conclusion: Since coronaviruses do not differ structurally to any great exent, the SARS-CoV-2 virus – as well as possible future mutations – will very likely be highly UV sensitive, so that common UV disinfection procedures will inactivate the new SARS-CoV-2 virus without any further modification.

Digital light processing of SiC ceramic from allylhydridopolycarbosilane with limited acrylate monomers

Digital light processing of ceramic precursor was used to prepare SiC rich ceramic parts in this study. In order to achieve appropriate light curing rate, the ceramic precursor allylhydropolycarbosilane (LHBPCS) was mixed with acrylate monomers tripropylene glycol diacrylate and trimethylolpropane triacrylate. The content of acrylate monomers was optimized to increase the ceramic yield and reduce the shrinkage during pyrolysis. According to the results of thermogravimetric analysis and photolithography experiment, 15 wt% acrylate monomers was appropriate. 330 mJ/cm2 UV irradiation dose was selected for every layer with a thickness of 25 μm, and green bodies with different shapes were successfully printed. During pyrolysis, these printed parts changed from transparent yellow to black accompanying uniform shrinkage. At 1000 °C, the shrinkage was 24.0–26.0%, and crack-free SiC rich ceramic parts with density of 2.11 g/cm3 and chemical formula of SiC1.31O0.26 were obtained.

Human mitochondrial RNA modifications associate with tissue-specific changes in gene expression, and are affected by sunlight and UV exposure.

RNA-DNA differences (RDD) have previously been identified in the human mitochondrial RNA (mt-RNA) transcripts, yet their functional impact is poorly understood. By analyzing 4928 RNA-seq samples from 23 body sites, we found that mtDNA gene expression negatively correlated with the levels of both m1A 947 16 S rRNA modification (mtDNA position 2617) and the m1A 1812 ND5 mRNA modification (mtDNA position 13,710) in 15 and 14 body sites, respectively. Such correlation was not evident in all tested brain tissues, thus suggesting a tissue-specific impact of these modifications on mtDNA gene expression. To assess the response of the tested modifications to environmental cues, we analyzed pairs of skin samples that were either exposed to the sun or not. We found that the correlations of mtDNA gene expression with both mt-RNA modifications were compromised upon sun exposure. As a first step to explore the underlying mechanism, we analyzed RNA-seq data from keratinocytes that were exposed to increasing doses of UV irradiation. Similar to sun exposure, we found a significant decrease in mtDNA gene expression upon increase in UV dosage. In contrast, there was a significant increase in the m1A 947 16 S rRNA modification levels upon elevation in UV dose. Finally, we identified candidate modulators of such responses. Taken together, our results indicate that mt-RNA modifications functionally correlate with mtDNA gene expression, and responds to environmental cues, hence supporting their physiological importance.

Analysis of Mechanical Property Degradation of Outdoor Weather-Exposed Polymers.

It is well known that many polymers are prone to outdoor weathering degradation. Therefore, to ensure the safety and integrity of the structural parts and components made from polymers for outdoor use, their weather-affected mechanical behavior needs to be better understood. In this study, the critical mechanical property for degradation was identified and modeled into a usable format for use in the virtual analysis. To achieve this, an extensive 4-year outdoor weathering test was carried out on polycarbonate (PC), polypropylene (PP), polybutylene terephthalate (PBT), and high-density polyethylene (HDPE) polymers up to a total UV irradiation of 1020 MJ/m2 at a 315~400 nm wavelength. In addition, tensile tests were performed by collecting five specimens for each material at every 60 MJ/m2 interval. With the identification of fracture strain retention as the key performance index for mechanical property degradation, a fracture strain retention function was developed using logistic regression analysis for each polymer. In addition, a method for using fracture strain retention function to establish a mechanical property degradation dataset was proposed and successfully tested by performing weathering FE analysis on the virtual automotive collision behavior of a PC part under intermittent UV irradiation doses. This work showed the potential of using fracture strain retention function to predict the performance of polymeric components undergoing mechanical property degradation upon outdoor weathering.

Phytoplankton Biodiversity in Two Tropical, High Mountain Lakes in Central Mexico

Lakes El Sol and La Luna, inside the crater of the Nevado de Toluca volcano, Central Mexico, are the only two perennial high mountain lakes [HML] in the country. HML are considered among the most comparable ecosystems worldwide. These lakes are “extreme” environments with diluted, oligotrophic, and cold waters exposed to high UV radiation doses. In this paper, we document the phytoplankton species inhabiting these two extreme tropical ecosystems. The phytoplankton diversity of Lakes El Sol and La Luna is low compared to the global phytoplankton diversity from other Mexican inland waters. Nonetheless, the phytoplankton diversity turned out to be high compared to other HML worldwide, both temperate and tropical. The phytoplankton community in Lake El Sol was formed by 92 morphospecies and in Lake La Luna by 63; in both, the most diverse groups were Bacillariophyceae and Chlorophyceae. All species found in Lake La Luna were present in Lake El Sol, but 29 species present in Lake El Sol were absent in Lake La Luna. Nevertheless, 57% of the most frequent and abundant taxa in Lake El Sol were rare in La Luna, and 47% of the most frequent and abundant taxa in Lake La Luna were rare in Lake El Sol. Associated with their shallowness and polymictic thermal pattern, 87.5% of the Bacillariophyceae morphospecies are tychoplantonic, while the remaining 12.5% are truly planktonic. Dominant taxa were Chlorophyceae in Lake El Sol and Cyanobacteria in Lake La Luna. The most extreme conditions of Lake La Luna (ultraoligotrophy, very low pH, high ultraviolet radiation) most likely explained the differences in the dominant species composition and lower species richness compared to Lake El Sol. Herein, we provide for the first time a taxonomic list of the phytoplankton community of Lakes El Sol and La Luna. This information constitutes a baseline needed to use these HML as sentinels of global and climate change.

The effect of synergism in seed treatment with electron-donor solution and UV irradiation

The paper shows the synergistic effect of simultaneous exposure to ultraviolet radiation and electron-donor solution on the seed germination of grain crops. When the seed is moistened, the biochemical processes that control the growth of the root system and stem are activated in them. The purpose of the study is to determine the presence of a synergistic effect on seed germination of grain crops with simultaneous exposure to ultraviolet radiation and electron-donor solution. It was found out that the oxidation-reduction potential (ORP) is affected by the time of seed soaking, the weight of seeds and the type of grain crops. The best soaking time turned out to be 18 hours, the greatest negative ORP was in oat seeds -510 mV (100%), in barley and rye it was 487mV (95%), in wheat - 462 mV (91%). The greatest synergistic effect was obtained with a 10-minute exposure to UV irradiation of seeds at a dose of 1,882 ± 14.1 J/m2, followed by soaking in an electron-donor solution. In this case, the height of the sprouts was 108.8 mm (100%). At an exposure of 15 min (UV radiation dose 2.813 ±18.3 J/m2), the effect was slightly lower. The height of the sprouts was 107.6 mm (98.9%)

Enhancing photosensitivity via the assembly of a uranyl coordination polymer.

Synergistic assembly of uranyl centres and luminescent 2,6-bis(pyrazol-1-yl)pyridine-4-carboxylates (bppCOOH) gives rise to a uranyl coordination polymer, namely U-bppCOO, which exhibits a luminescence quenching response toward UV or X-ray irradiation doses. Notably, the photosensitivity of U-bppCOO has been significantly enhanced via metal-ligand assembly compared with that of the naked bppCOOH ligand.

Study of the effectiveness of the use of closed-type UV-recirculators for air disinfection in enclosed space

Introduction. The research is devoted to assessing the results of our studies of indoor air concerning microbial contamination during the operation of a UV recirculator with different modes (different UV doses). Also, a theoretical calculation of the influence of the ratio of the capacity of the UV recirculator to the air volume of the treated room on the efficiency of air disinfection has been made. Materials and methods. The study of indoor air in terms of total bacterial count (TBC), including coccal microflora and yeast and mould fungi, were carried out. Air sampling and evaluation were carried out under the requirements of Methodical guidelines MUK 4.2.2942-11 “Methods of sanitary and bacteriological studies of environmental objects, air and sterility control in medical institutions”. The evaluation of the results was carried out following R 3.5.1904-04, "The use of ultraviolet bactericidal radiation for disinfection of indoor air". During the study, agar culture media were used: Sabouraud agar, yolk-salt agar (YSA), meat-peptone agar (MPA), nutrient agar with the addition of 5% sheep blood (blood agar), bismuth sulfite agar, XLD-agar, cetrimide-agar, “Shine” agar, Endo agar. Results. As a result of the studies carried out, it was shown that a dose of UV irradiation of the order of 12-15 mJ/cm2 leads to an insignificant change in the concentration of bacteria (TBC) and fungi in the air (the efficiency was 58% and 69%, respectively). UV doses of the order of 25-30 mJ/cm2 significantly reduce the concentration of bacteria (TBC) and fungi in the air (efficiency was 99.99% and 99.4%, respectively). A theoretical calculation showed that it is practical to use a UV recirculator of such a capacity that provides an air exchange rate in the room of at least 4 (with ventilation operating at a rate of at least 2). Conclusion. To effectively use UV recirculators in enclosed spaces against bacteria and fungi, it is necessary to use models that provide a UV dose of at least 25-30 mJ/cm2. In contrast, their air capacity should provide an air exchange rate of at least 4.

The Ground-Based BIOMEX Experiment Verification Tests for Life Detection on Mars.

The success of an astrobiological search for life campaign on Mars, or other planetary bodies in the Solar System, relies on the detectability of past or present microbial life traces, namely, biosignatures. Spectroscopic methods require little or no sample preparation, can be repeated almost endlessly, and can be performed in contact or even remotely. Such methods are therefore ideally suited to use for the detection of biosignatures, which can be confirmed with supporting instrumentation. Here, we discuss the use of Raman and Fourier Transform Infrared (FT-IR) spectroscopies for the detection and characterization of biosignatures from colonies of the fungus Cryomyces antarcticus, grown on Martian analogues and exposed to increasing doses of UV irradiation under dried conditions. The results report significant UV-induced DNA damage, but the non-exceeding of thresholds for allowing DNA amplification and detection, while the spectral properties of the fungal melanin remained unaltered, and pigment detection and identification was achieved via complementary analytical techniques. Finally, this work found that fungal cell wall compounds, likely chitin, were not degraded, and were still detectable even after high UV irradiation doses. The implications for the preservation and detection of biosignatures in extraterrestrial environments are discussed.

100 YEARS OF VITAMIN D: Dose-response for change in 25-hydroxyvitamin D after UV exposure: outcome of a systematic review.

A systematic review of publications addressing change in vitamin D status (25-hydroxyvitamin D (25OHD)) after exposure to UV radiation identified 2001 independent peer-reviewed publications. Of these, 21 used artificial sources of UV radiation, met all inclusion criteria and were quality assured; 13 publications used solar radiation and met sufficient inclusion criteria to be retained as supporting evidence; 1 further included publication used both solar and artificial sources. The review consistently identified that low dose, sub-erythemal doses are more effective for vitamin D synthesis than doses close to a minimum erythema dose; increasing skin area exposed increases the amount of vitamin D synthesised although not necessarily in a linear manner; constant dosing leads to a dose-dependent plateau in 25OHD, and dose–response is greatest at the start of a dosing regime; there is a large interpersonal variation in response to UV exposure. Fourteen of the studies using artificial sources of radiation were used to determine a dose–response relationship for change in 25OHD on whole-body exposure to repeated sub-erythemal doses of UV radiation, taking the form Δ25OHD (nmol/L) = A ln(standard vitamin D dose) + B. This helps quantify our understanding of UV as a source of vitamin D and enables exposure regimes for safe synthesis of vitamin D to be assessed. Specific studies of people with pigmented skin (Fitzpatrick skin types 5 and 6) were rare, and this dose–response relationship is only applicable to white-skinned individuals as skin type is a determinant of response to UV radiation. Findings provide information for vitamin D guidance updates.

Induction of gynogenesis by heterogenous sperm and cold shock treatment in Epinephelus fuscoguttatus

Gynogenesis is a chromosome set manipulation technique to accelerate inbred lines used for genomics analysis and genetic breeding. Groupers are hermaphroditic fish whereby they sexually mature as females and subsequently change sex to male. Production of the homozygous inbred lines of groupers will take a prolonged period with the traditional method. This study presents the meiotic gynogenesis induction of the commercially cultured grouper Epinephelus fuscoguttatus, using a combination of ultraviolet-irradiated heterogenous sperm of E. lanceolatus and recovering second polar body by cold-shock treatment. DNA ploidy verification by flow cytometry showed that the haploid larvae exhibited abnormalities and diploids developed normally. The results indicated that UV irradiation doses at 150 ​mJ/cm2 produced 100% haploid larvae. The high percentage (52.1% ​± ​8.50%) of gynogenetic diploidy was induced by cold shock at 5–7 ​°C for 10 ​min, initiated 6 ​min after fertilization. The gynogenetic offspring presented 48 chromosomes (2n ​= ​48t) and were morphologically identical to maternal chromosomes. Cytological and morphological evidence suggested that gynogenetic individuals were successfully obtained. Histological examination revealed that the gynogens gonads could develop into ovaries. Summarily, the gynogenesis technique was very representative of creating inbred lines in groupers. The successful production of this inbred lines is of great significance in grouper genetic breeding and genomic analysis.