Twenty prepubertal rabbit does, 12–14 weeks of age, were randomly allocated to a treatment group receiving 72 IU pregnant mares serum gonadotrophin (PMSG) and 36 IU human chorionic gonadotrophin (HCG) in a single i.m. dose, or to a control group receiving an injection of physiological saline. At 48 h after administration of treatment or control injections, animals were exposed to males to determine if breeding receptivity had been induced. Does were sacrificed at 96 h post treatment and their reproductive tracts were assessed for hormonal stimulation. The ovaries of treated does had significantly higher numbers of ovulation sites than did those of control animals (17.6 vs. 2.1; Fisher's LSD0.01). One doe in the treatment group and one doe in the control group showed mating receptivity. There was no age, weight, dam or replication effect. All does in the treatment group ovulated. This study shows that prepubertal rabbits, 12–14 weeks of age, can be induced to ovulate using a single, combined dose of PMSG and HCG. Although this treatment does not stimulate estrus behavior, the protocol can be employed to simplify procedures and reduce the handling of animals when inducing superovulation for the purpose of oocyte retrieval or artificial insemination.