Bacillus species and organomineral fertilizers can improve various crop responses. The objective of this study was to evaluate the efficiency of organomineral fertilizer with B. subtilis and B. licheniformis, in comparison to mineral fertilizer, on soybean growth, leaf nutrient content, grain yield, and soil characteristics. Two soybean field experiments (305 km apart) were set up in southeastern Brazil. Two sources of fertilizers (inoculated granulated organomineral, 5-26-0 + 0-0-32 N-P2O5-K2O, and coated mineral fertilizer, 10-52-0 + 0-0-60 N-P2O5-K2O), and four doses of P plus K (no fertilizer, 50, 75, 100 kg ha−1 of each P2O5 and K2O) were applied. At 60 days after sowing, plant height and leaf analysis were assessed. Weight of 1000 seeds, grain yield, and soil analysis were evaluated (120 days after sowing). In both fields, there was interaction between source and P plus K dose factors for soybean grain yield. In Site 1, the highest grain yield (4,543.69 kg ha−1) was observed for 100 kg ha−1 of P2O5 and K2O dose of coated mineral fertilizer; in Site 2, the highest grain yield (4,638.55 kg ha−1) was observed for 75 kg ha−1 of P2O5 and K2O dose of organomineral fertilizer. These factors also affected N, P, and K leaf contents. Less effects were observed on plant height, weight of 1000 seeds, and soil characteristics. Inoculated organomineral fertilizer can be used for total or partial supply of nutrients without a clear difference from mineral fertilization. Smaller doses of inoculated organomineral fertilizer could be used without compromising soybean grain yield.