Dos Pinhais Research Articles

Consequences of enamel preparation with sodium hypochlorite, polyacrylic and phosphoric acids for the bonding of brackets with resin-modified glass ionomer cements

The aim of this study was to evaluate the effects of deproteinization with 5.25% sodium hypochlorite (NaOCl) prior to enamel conditioning with 10% polyacrylic acid (PAA) and 35% phosphoric acid (PA) on the bond strength (BS) of brackets bonded with resin-modified glass ionomer cement (RMGIC). One hundred human premolars extracted for orthodontic reasons were divided into 5 groups (n = 20 in each group): G1 (control), enamel conditioning with PA, application of adhesive and bonding of brackets with TransbondTM XT composite resin (3M/Unitek, Monrovia, CA, USA); G2, enamel conditioning with PAA and bonding with RMGIC (Fuji OrthoTM LC, GC America, Alsip, IL, USA); G3, NaOCl-treated enamel, conditioning with PAA and bonding with RMGIC; G4, enamel conditioning with PA and bonding with RMGIC; and G5, NaOCl-treated enamel, conditioning with PA and bonding with RMGIC. Once the brackets were bonded, the teeth were stored in distilled water for 24 hours at room temperature and pressure until being subjected to shear stress in a Universal Mechanical Testing Machine (EMIC® DL 500, São José dos Pinhais, PR, Brazil). The BS value was higher in G1 (17.08 ± 6.39) than in any of the experimental groups (p < 0.05). No statistically significant differences were noted between groups using RMGIC (p > 0.05), except between G3 (9.86 ± 2.90) and G5 (5.00 ± 2.49). No statistically significant differences were noted between the mean Adhesive Remnant Index values among the evaluated groups (p > 0.05). Conclusion: The use of NaOCl combined with PAA increased the BS of brackets bonded with RMGIC. The deproteinization of the group treated with PA reduced the shear bond strength of the brackets.

Effect of different concentrations of papain gel on orthodontic bracket bonding

BackgroundThe objective of this study was to verify the hypothesis that enamel deproteinization with papain gel at concentrations of 2%, 4%, 6%, 8%, and 10% increases shear bond strength as concentration increases.MethodsA total of 180 bovine mandibular permanent incisors were used, divided into six groups (n = 30), and denominated as follows: group 1 is the control group (CG) in which brackets are bonded with resin-modified glass ionomer cement (RMGIC) according to the manufacturer's recommendations and groups 2, 3, 4, 5, and 6 have brackets bonded with RMGIC after enamel deproteinization with papain gel at concentrations of 2%, 4%, 6%, 8%, and 10%, respectively. After bonding, teeth were immersed in artificial saliva and kept at a temperature of 37°C for 24 h. Mechanical tests were then performed in a universal mechanical test machine EMIC DL 5000 (Sao Jose dos Pinhais, Brazil). Values obtained were submitted to analysis of variance and then to Tukey's test (p < 0.05).ResultsThe results demonstrated that groups 5 and 6 showed the highest shear bond strength, differing statistically from the other groups (p < 0.05). CG with no papain gel used showed the lowest value and in turn showed no differences for groups 2, 3, and 4. As regards adhesive remnant index, CG showed statistical differences from the others. Groups 2, 3, 4, 5, and 6, in which papain gel was used, presented no statistical differences among them (p > 0.05).ConclusionsIt was concluded that enamel deproteinization with 8% and 10% papain gel increases shear bond strength of orthodontic brackets bonded with RMGIC.

Comparison of static friction with self-ligating, modified slot design and conventional brackets

To compare the static frictional forces generated at the bracket/wire interface of stainless steel brackets with different geometries and angulations, combined with orthodontic wires of different diameters. The frictional forces were evaluated with three different types of metal brackets: a passive self-ligating (SmartClipTM, 3M/Unitek, Monrovia, USA), with a modified slot design (Mini Uni TwinTM, 3M/Unitek, Monrovia, USA) and conventional (Kirium, Abzil, São José do Rio Preto, Brazil). The samples were mounted in a testing device with three different angulations and tested with 0.014" and 0.018" stainless steel wires (American Orthodontics, Sheboygan, USA). The static frictional force was measured using a universal testing machine (DL 500, EMIC®, São José dos Pinhais, Brazil) with a crosshead speed of 1 mm/min. Statistical analysis was performed by two-way ANOVA followed by Bonferroni's post hoc test. There was a significant difference (p<0.05) in static friction when the three types of brackets were tested with the same wire size. The wire diameter influenced friction only when the brackets had a 10º angulation (p<0.05). The angulation influenced friction (p<0.05) when the brackets were associated with a 0.018" wire. Brackets with a modified slot design showed intermediate static frictional force values between the conventional and self-ligating brackets tested.

Comparison of static friction with self-ligating, modified slot design and conventional brackets

ObjectiveTo compare the static frictional forces generated at the bracket/wire interface of stainless steel brackets with different geometries and angulations, combined with orthodontic wires of different diameters. Material and MethodsThe frictional forces were evaluated with three different types of metal brackets: a passive self-ligating (SmartClipTM, 3M/Unitek, Monrovia, USA), with a modified slot design (Mini Uni TwinTM, 3M/Unitek, Monrovia, USA) and conventional (Kirium, Abzil, São José do Rio Preto, Brazil). The samples were mounted in a testing device with three different angulations and tested with 0.014" and 0.018" stainless steel wires (American Orthodontics, Sheboygan, USA). The static frictional force was measured using a universal testing machine (DL 500, EMIC®, São José dos Pinhais, Brazil) with a crosshead speed of 1 mm/min. Statistical analysis was performed by two-way ANOVA followed by Bonferroni's post hoc test. ResultsThere was a significant difference (p<0.05) in static friction when the three types of brackets were tested with the same wire size. The wire diameter influenced friction only when the brackets had a 10º angulation (p<0.05). The angulation influenced friction (p<0.05) when the brackets were associated with a 0.018" wire. ConclusionBrackets with a modified slot design showed intermediate static frictional force values between the conventional and self-ligating brackets tested.

Uncommon mandibular osteomyelitis in a cat caused by Nocardia africana

BackgroundNocardiosis is an unusual infection in companion animals characterized by suppurative to pyogranulomatous lesions, localized or disseminated. Cutaneous-subcutaneous, pulmonary and systemic signs are observed in feline nocardiosis. However, osteomyelitis is a rare clinical manifestation in cats. Nocardia cyriacigeorgica (formerly N. asteroides sensu stricto), Nocardia brasiliensis, Nocardia otitidiscaviarum, and Nocardia nova are the most common pathogenic species identified in cats, based on recent molecular classification (16S rRNA gene). The present report is, to our knowledge, the first case of mandibular osteomyelitis in a cat caused by Nocardia africana, diagnosed based upon a combination of methods, including molecular techniques.Case presentationA one-year-old non-neutered female cat, raised in a rural area, was admitted to the Companion Animal Hospital-PUCPR, São José dos Pinhais, State of Paraná, Brazil, with a history a progressive facial lesion, difficulty apprehending food, loss of appetite, apathy and emaciation. Clinical examination showed fever, submandibular lymphadenitis, and a painless, 8 cm diameter mass, which was irregularly-shaped, of firm consistency, and located in the region of the left mandible. The skin around the lesion was friable, with diffuse inflammation (cellulitis), multiple draining sinuses, and exudation of serosanguinous material containing whitish “sulfur” granules.Diagnosis was based initially in clinical signs, microbiological culture, cytological, and histopathological findings, and radiographic images. Molecular sequencing of 16S rRNA of isolate allowed diagnosis of Nocardia africana. Despite supportive care and antimicrobial therapy based on in vitro susceptibility testing the animal died.ConclusionThe present report describes a rare clinical case of feline osteomyelitis caused by Nocardia africana, diagnosed based upon a combination of clinical signs, microbiological culture, cytological and histopathological findings, radiographic images, and molecular methods. The use of modern molecular techniques constitutes a quick and reliable method for Nocardia species identification, and may contribute to identification to new species of Nocardia that are virulent in cats.

Prevalência da hepatite viral C em adultos usuários de serviço público de saúde do município de São José dos Pinhais - Paraná

We aimed to investigate the prevalence of Hepatitis C in adult users of the Public Health Services of São José dos Pinhais - Paraná. We performed an epidemiological survey with a probabilistic sample of 5,017 volunteers who answered a questionnaire and were submitted to an anti-HCV quick test. Data were organized using Epi-info 3.5.1. The association between the presence of the disease and the factors of interest in the study were evaluated by the Chi-square test. We used a Logistic Regression Adjusted Model for risk factor analysis and the Wald test for decision making on the importance of the risk factors. The absolute frequency of positive anti-HCV was 13, with a prevalence of 0.30%, (IC = 0.12% - 0.40%). A higher probability of the disease was reported in males (p = 0.008) and in single, separated and widowed subjects (p = 0.045); in subjects with prior HCV symptoms (p < 0.001) and a previous blood transfusion (p < 0.001); and with the presence of a tattoo (p = 0.033). Drug abuse, blood transfusion and age > 40 years increased the risk for disease, regardless of sex, age and marital status. We found a low prevalence of Hepatitis C, albeit expected for Southern Brazil. Our results did not differ from other studies as to contamination risks. The study may contribute to highlight the importance of Hepatitis C, the need to implement strategies to cope with it, and stimulate better understanding of Hepatitis C.

Spatial–temporal composition of Chrysomelidae (Insecta: Coleoptera) communities in southern Brazil

We examined the Chrysomelidae communities from representative sites of different ecosystems in Paraná State, Brazil, comparing their spatial and temporal changes. Sampling was undertaken using malaise traps at eight sites during a 2-year period. Chrysomelidae represented 19.1% of all the Coleoptera sampled, with a total of 7611 specimens and 465 species. Galerucinae was the most abundant and richest subfamily. Ponta Grossa was the most abundant site, São José dos Pinhais the richest, Fênix the poorest, Jundiaí do Sul the most uniform, and Antonina had the highest dominance of just a single species. Approximately 79% of the species were represented by fewer than 10 specimens and the 10 most abundant species represented 44.7% of all chrysomelids collected. None of the species were collected at all of the sites, but 325 were unique, reflecting the low similarity among sites. Sites with similar phytophysiognomies were more similar in terms of their Chrysomelidae species compositions. Similarities between years were slightly higher than similarities between sites.

First record of Spintherophyta semiaurata (Klug) (Coleoptera: Chrysomelidae) damaging strawberry flowers

Spintherophyta semiaurata (Klug) is reported for the first time damaging flowers of strawberry (Fragaria x ananassa) in the municipality of São José dos Pinhais, State of Parana, Brazil.

&lt;b&gt;Anurofauna da Colônia Castelhanos, na Área de Proteção Ambiental de Guaratuba, Serra do Mar paranaense, Brasil&lt;/b&gt;&lt;br&gt;doi: 10.5007/2175-7925.2010v23n2p123

A riqueza de espécies e a distribuição espacial dos anuros foram estudadas ao longo de 12 meses em uma região de Floresta Atlântica no Município de São José dos Pinhais, estado do Paraná, Brasil. Foram registradas 32 espécies, distribuídas em dez famílias: Brachycephalidae (2), Bufonidae (2), Centrolenidae (1), Cycloramphidae (1), Hemiphractidae (1), Hylidae (18), Hylodidae (1), Leiuperidae (2), Leptodactylidae (3) e Microhylidae (1). A área aberta apresentou 16 espécies, enquanto a área de borda apresentou 17 e o interior de floresta 20. Quanto à utilização dos microhabitats, as espécies foram encontradas: 1) no solo (oito); 2) na água (duas); 3) estrato inferior (onze); 4) estrato intermediário (cinco); 5) estrato superior (quatro). Cinco foram abundantes (15,6%), doze foram comuns (37,5%) e quinze foram raras (46,9%). A maioria das espécies é característica de áreas florestais, reforçando a importância da conservação da Floresta Atlântica.

Qualidade fisiológica de sementes de camomila [Chamomilla recutita (L.) Rauschert] após envelhecimento acelerado e estresse salino

O objetivo do trabalho foi verificar a qualidade fisiológica de sementes da camomila submetidas a diferentes tempos de envelhecimento acelerado e ao estresse salino. Os experimentos foram realizados no Laboratório de Técnica de Sementes na PUCPR em São José dos Pinhais, Paraná. Os tempos de envelhecimento acelerado foram de 0, 24, 48, 72 e 96 h e o estresse salino foi induzido por soluções de cloreto de sódio com potenciais osmóticos de 0; -0,2; -0,4; -0,6; -0,8; -1,0 MPa, ambos com quatro repetições. Foram avaliados a germinação, o índice de velocidade de germinação (IVG), o tempo médio de germinação (TMG), a velocidade media de germinação (VMG) e a entropia. O delineamento experimental adotado foi o de blocos casualizados . Concluiu-se que as sementes de camomila tiveram sua qualidade fisiológica afetada após 24h de envelhecimento acelerado e sob estresse salino causado por solução de potencial osmótico de -0,6 MPa

Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil

Brazilian spotted fever (BSF) is a vector-borne zoonosis caused by Rickettsia rickettsii bacteria. Dogs can be host sentinels for this bacterium. The aim of the study was to determine the presence of antibodies against Rickettsia spp. in dogs from the city of São José dos Pinhais, State of Paraná, Southern Brazil, where a human case of BSF was first reported in the state. Between February 2006 and July 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirect immunofluorescence assay (IFA) against R. rickettsii and R. parkeri. All sera that reacted at least to one of Rickettsia species were tested against the six main Rickettsia species identified in Brazil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis. Sixteen samples (4.4%) reacted to at least one Rickettsia species. Among positive animals, two dogs (15.5%) showed suggestive titers for R. bellii exposure. One sample had a homologous reaction to R. felis, a confirmed human pathogen. Although Rickettsia spp. circulation in dogs in the area studied may be considered at low prevalence, suggesting low risk of human infection, the present data demonstrate for the first time the exposure of dogs to R. bellii and R. felis in Southern Brazil.

TROCLEOPLASTIA POR RESSECÇÃO ASSOCIADA À TRANSPOSIÇÃO DA TUBEROSIDADE TIBIAL E SOBREPOSIÇÃO DA FÁSCIA LATA PARA O TRATAMENTO DA LUXAÇÃO PATELAR EM CÃES

Neste estudo retrospectivo é relatada a experiência com a técnica cirúrgica composta de trocleoplastia por ressecção associada à transposição da tuberosidade tibial e sobreposição da fáscia lata no tratamento da luxação patelar em cães. Vinte e sete cães adultos, de ambos os sexos, participaram deste estudo. Os animais apresentavam diferentes graus de luxação patelar, após a intervenção cirúrgica foram acompanhados por período que variou de três meses a dois anos. Apenas um teve recorrência da luxação. O retorno à função do membro afetado variou de uma semana a quatro meses de período pós-operatório. A reconstituição cirúrgica foi de fácil execução e os resultados obtidos neste estudo retrospectivo permitem concluir que a tática cirúrgica empregada é apropriada para luxações patelares, tanto mediais quanto laterais.

Diatomáceas perifíticas da represa Itaqui, São José dos Pinhais, Paraná: Fragilariales, Eunotiales, Achnanthales e Gomphonema Ehrenberg

Realizou-se o levantamento florístico das espécies de diatomáceas perifíticas incluídas em Fragilariales, Eunotiales, Achnanthales e Gomphonema Ehrenberg, em uma represa do Rio Itaqui, localizada no município de São José dos Pinhais, PR. Lâminas de vidro foram utilizadas como substrato, as quais permaneceram submersas durante 30 dias no ambiente. Quatro coletas trimestrais, de maio de 2008 a fevereiro de 2009, foram realizadas para análise qualitativa. Amostras foram coletadas para estimar o estado trófico da represa, a qual foi classificada como hipereutrófica. Determinaram-se 49 táxons, sendo 44 em nível infragenérico. Doze novos registros foram adicionados à diatomoflórula paranaense: Fragilaria socia, Eunotia rabenhorstiana var. elongata, E. soleirolii, E. ventricosa var. brevis, Gomphonema parvulum f. saprophilum, G. pumilum, Achnanthes minuscula, Nupela wellneri, Planothidium frequentissimum, Ulnaria sp., Eunotia sp.2 e Gomphonema sp.2. Os táxons determinados em nível genérico, Ulnaria sp., Eunotia sp.1, Eunotia sp.2, Gomphonema sp.1 e Gomphonema sp.2 devem ser alvo de estudos posteriores, pois nenhuma forma semelhante foi registrada na literatura consultada.

Photoelastic Stress Analysis in Screwed and Cemented Implant-Supported Dentures With External Hexagon Implants

The aim of this study was to assess the stress distribution of the retention systems (screwed and cemented) for implant-supported fixed partial dentures by means of photoelastic method. Two models were made of photoelastic resin PL-2 with 2 implants (phi = 4.00 x 10 mm) located in the second premolar and molar region in each photoelastic model, varying the retention system (screwed and cemented). The implant-supported fixed partial dentures were standardized and made of Ni-Cr alloy. Axial and oblique (45 degrees) forces of 100 N were applied on the occlusal surface by means of a Universal Testing Machine (EMIC-DL 3000; São José dos Pinhais, Paraná, Brazil). The results were observed and photographed in the field of a circular polariscope and qualitatively analyzed with the aid of computer software (Adobe Photoshop, San Jose, CA). The screw retention system presented the highest number of fringes when the loads were applied on the premolar, pontic, and molar and showed this behavior in all load applications, under axial and oblique loads. It was concluded that there was a better stress distribution and lower magnitude of stress on the cemented implant-supported dentures, under axial and oblique loads. Oblique load caused an increase in stress concentrations in all the models.

Surveillance of canine visceral leishmaniasis in a disease-free area

Leishmaniasis is an important re-emergent parasitosis worldwide, particularly in tropical countries. There are no reports of autochthonous disease in the State of Paraná, southern Brazil. No surveillance has been carried out in the most populated areas such as the city of Curitiba and its surroundings. The purpose of the present study was to determine the seroprevalence of visceral leishmaniasis in dogs at the Center for Zoonosis Control of São José dos Pinhais, Paraná, before euthanasia. Enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT) were used to detect antibody levels against Leishmania sp. in dog sera. Imprints of the popliteal lymph nodes that were also randomly collected from 50 dogs with suspected clinical signs of visceral leishmaniasis, and evaluated under light microscopy for the detection of amastigote forms, were negative. A total of 364 dog samples were tested. The results showed only one positive sample (0.0027%) by ELISA test but negative by IFAT, however, the dog had no clinical signs. Random surveillance of dog populations from several districts of a metropolitan area may be a means of preventing Leishmania spreading. Based on our results, the city of Curitiba and its metropolitan area were considered at low risk for visceral leishmaniasis.

A new species of Aurigoniella Takiya, Mejdalani &amp; Felix (Hemiptera, Cicadellidae, Cicadellini) from southern Brazil

Aurigoniella meridionalis, sp. nov. is described and illustrated from Morretes and São José dos Pinhais, Paraná State, southern Brazil. It can be easily distinguished from the Aurigoniella type-species, A. dutrai, by its distinct color pattern, smaller size, shorter crown, hindleg knees attaining lateral lobe of pronotum, aedeagus broadest height around mid-length, and paraphyses ramus biramous.

Ocorrência de Leishmania spp. em felinos do município de AraÇatuba, SP

Brazilian Spotted Fever (BSF) is an often fatal zoonosis caused by the obligate intracellular bacterium Rickettsia rickettsii. The disease is generally transmitted to humans by Amblyomma spp. ticks. Serological evidence of past infection by R. rickettsii has been reported in horses, but the pathogenicity of R. rickettsii in horses remains unknown. Cart horses are still widely used in urban and urban fringe areas in Brazil, and these animals may constitute suitable sentinels for BSF human in these areas, for example, in Sao Jose dos Pinhais, where the first BSF human case in the state of Parana was diagnosed. Serum samples were randomly obtained from 75 cart horses between April 2005 and June 2006 and were tested by means of the indirect immunofluorescence assay (IFA) for antibodies against rickettsia of the spotted fever group. A total of 9.33% of the animals were considered positive, with titers ranging from 64 to 1,024. These results indicate the presence of the agent in such areas, although at low rates.

Surveillance of canine visceral leishmaniasis in a disease-free area Vigilância da leishmaniose visceral canina em área indene

Leishmaniasis is an important re-emergent parasitosis worldwide, particularly in tropical countries. There are no reports of autochthonous disease in the State of Parana, southern Brazil. No surveillance has been carried out in the most populated areas such as the city of Curitiba and its surroundings. The purpose of the present study was to determine the seroprevalence of visceral leishmaniasis in dogs at the Center for Zoonosis Control of Sao Jose dos Pinhais, Parana, before euthanasia. Enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT) were used to detect antibody levels against Leishmania sp. in dog sera. Imprints of the popliteal lymph nodes that were also randomly collected from 50 dogs with suspected clinical signs of visceral leishmaniasis, and evaluated under light microscopy for the detection of amastigote forms, were negative. A total of 364 dog samples were tested. The results showed only one positive sample (0.0027%) by ELISA test but negative by IFAT, however, the dog had no clinical signs. Random surveillance of dog populations from several districts of a metropolitan area may be a means of preventing Leishmania spreading. Based on our results, the city of Curitiba and its metropolitan area were considered at low risk for visceral leishmaniasis.

PREVALÊNCIA DA LEUCOSE ENZOÓTICA EM BOVINOS LEITEIROS CRIADOS NA REGIÃO METROPOLITANA DE CURITIBA – PARANÁ

Enzootic bovine leukosis (EBL) is an infectious, contagious disease caused by bovine leukemia virus (BLV), which is an oncogenic retrovirus of the family Retroviridae. This study evaluates the prevalence of BLV in dairy cattle raised in Curitiba and its surrounding areas. A total of 268 serum samples were collected from five different herds in the municipalities of Sao Jose dos Pinhais, Campina Grande do Sul, Pinhais, and Fazenda Rio Grande. An immunodiffusion (ID) test revealed that 151 (56,34%) animals were positive and 117 (43,66%) were negative.

Assessment of the flexural strength of two heat-curing acrylic resins for artificial eyes

Prosthetic eyes are artificial substitutes for the eyeball, made of heat-curing acrylic resin, serving to improve the esthetic appearance of the mutilated patient and his/her inclusion in society. The aim of this study was to assess the flexural strength of two heat-curing acrylic resins used for manufacturing prosthetic eyes. Thirty-six specimens measuring 64 x 10 x 3.3 mm were obtained and divided into four groups: acrylic resin for artificial sclera N1 (Artigos Odontológicos Clássico, São Paulo, SP, Brazil), heat-cure water technique (GI) and microwave-cured (GII); colorless acrylic resin for prosthetic eyes (Artigos Odontológicos Clássico, São Paulo, SP, Brazil), heat-cure water technique (GIII) and microwave-cured (GIV). Mechanical tests using three point loads were performed in a test machine (EMIC, São José dos Pinhais, PR, Brazil). The analysis of variance and the Tukey test were used to identify significant differences (p < 0.01). Groups GII and GIV presented, respectively, the highest (98.70 +/- 11.90 MPa) and lowest means (71.07 +/- 8.93 MPa), with a statistically significant difference. The cure method used for the prosthetic eye resins did not interfere in their flexural strength. It was concluded that all the resins assessed presented sufficient flexural strength values to be recommended for the manufacture of prosthetic eyes.