Dorsal Lingual Surface Research Articles

SEM study on the dorsal lingual surface of the large flying fox, Pteropus vampyrus.

The dorsal lingual surface of the large flying foxes were examined by scanning electron microscopy. Filiform, fungiform and vallate papillae were observed. The filiform papillae were distributed over the entire dorsal surface of the tongue. The filiform papillae notably differed in morphology by their location on the tongue and could be classified into 6 types: 1) scale-like, 2) giant trifid, 3) small crown-like, 4) large crown-like, 5) long conical and 6) short conical papillae. The fungiform papillae were present rounded bodies on the anterior 2/3 of the tongue. The large flying foxes showed the triangular arrangement of the three vallate papillae, with the apex of the triangle directed posteriorly. These findings indicate that the tongue of the large flying bat is similar to that of the lesser dog-faced fruit bat.

SEM study on the dorsal lingual surface of the lesser dog-faced fruit bat, Cynopterus brachyotis.

The dorsal lingual surface of the lesser dog-faced fruit bat was examined by scanning electron microscopy (SEM). Filiform (Fi), fungiform (Fu) and vallate papillae (V) were observed. The Fi papillae were distributed over the entire dorsal surface of the tongue. The Fi papillae notably differed in morphology by their location on the tongue and could be classified into 5 types: 1) crown-like papillae, 2) giant trifid papillae, 3) scale-like papillae, 4) small conical papillae, and 5) large conical papillae. The Fu papillae were present rounded bodies on the anterior 2/3 of the tongue. The lesser dog-faced fruit bat showed the triangular arrangement of the three V, with the apex of the triangle directed posteriorly.

Morphology of the dorsal lingual papillae in the black rhinoceros (Diceros bicornis).

The dorsal lingual surface of a black rhinoceros (Diceros bicornis) was examined by scanning electron microscopy. The tongue was about 30 cm in length. There were about 60 vallate papillae on both sides. Filiform, fungiform and vallate papillae were found. The filiform papillae were distributed over the entire dorsal surface of the tongue. The papillae had a hair-like shape. The fungiform papillae were round in shape, and more densely distributed on the lingual apex. No foliate papillae were seen on the dorsal surface. The vallate papillae were located on both sides of the posterior end of the lingual body. Each papilla was surrounded by a groove.

Morphology of the dorsal lingual papillae in the bush dog (Speothos venaticus).

The dorsal lingual surface of a bush dog (Speothos venaticus) was examined by scanning electron microscopy (SEM). The tongue was about 7 cm in length. Filiform, fungiform and vallate papillae were found. The filiform papillae were distributed over the entire dorsal surface of the tongue. Each filiform papillae on the apical surface of the tongue had several conical processes, in the midportion were larger than those on the apex in size. In the region of the vallate papillae, the filiform papillae had not the conical processes and more larger than those on the midportion of the tongue. The fungiform papillae were present rounded bodies and more densely distributed on the tip of the lingual apex. There were 5 vallate papillae on both sides. The vallate papillae were located on both sides of the posterior end of the lingual body. Each papilla was surrounded by a groove and a crescent pad. In the dorsal surfaces of the papillae, small conical papillae were observed.

Morphology of the dorsal lingual papillae in the barbary sheep, Ammotragus lervia.

The dorsal lingual surface of a barbary sheep (Ammotragus lervia) was examined by scanning electron microscopy (SEM). The tongue was about 20 cm in length. There were about 30 vallate papillae on both sides. Filiform, conical, fungiform and vallate papillae were found. The filiform papillae were distributed over the entire dorsal surface of the tongue, excepted for the lingual torus where conical papillae were present. The fungiform papillae were present rounded bodies, and more densely distributed as compared to that of the lingual body on the tip and ventral surface of lingual apex. No foliate papillae were seen on the dorsal surface. The vallate papillae were located on both sides of the midline in the caudal part. Each papilla was surrounded by a groove. These findings indicate that the tongue of the barbary sheep is similar to that of the formosan serow, japanese serow and blackbuck.

SEM study on the dorsal lingual surface of the flying squirrel, Petaurista leucogenys

The dorsal lingual surface of the flying squirrel was examined by scanning electron microscopy (SEM). Filiform (FI), fungiform (FU), foliate (FO) and vallate papillae (VA) were observed. The FI were distributed over the entire dorsal surface of the tongue. In the region of the VA, the FI appeared as giant cones. The FU were present as rounded bodies scattered over the dorsal surface of the tongue. They were relatively scarce and appear to be concentrated around the edges of the tongue. The FO were observed on the posterolateral regions of the tongue. The flying squirrel showed the triangular arrangement of the three VA, with the apex of the triangle directed posteriorly.

COMPARATIVE LIGHT AND SCANNING ELECTRON MICROSCOPIC STUDIES ON THE TONGUE OF THE NILE CROCODILE, CROCODYLUS NILOTICUS AND THE DESERT FAT SAND RAT, PSAMMOMYS OBESUS

The dorsal lingual surface of the Nile crocodile, Crocodylus niloticus, a carnivorous freshwater reptilian animal and the desert fat sand rat, Psammomys obesus, a herbivorous mammal, were examined by the light and Scanning Electron microscopes. Filiform (Fl), fungiform (FU) and circumvallate papillae (CI) were observed in the tongue of the Nile crocodile while the sand rat tongue contained only, (Fl) and (FU) papillae. In the crocodile the fungiform papillae were scattered in clusters among the filiform ones and contained taste buds. In the fat sand rat, however, the filiform papillae were denselydistributed all over the dorsal surface of the lingual body and flowershaped (FU) papillae were scarcely found in between. Histologically, the dorsal lingual epithelium revealed differences between the two animals. In the crocodile it consisted of a stratified squamous epithelium while in the fat sand rat it consisted of a keratinized stratified squamous epithelium. Taste buds were found in the lingual epithelium of the crocodile but completely absent in that of the fat sand rat. The lingual submucosa of the crocodile contained a network of melanophores and numerous lymphocytic aggregations which werecompletely absent in that of the fat sand rat. The mucous glands were present in the ventral lingual mucosa of the fat sand rat but completely absent in that of the crocodile. The tongue of the crocodile was supported by a cartilaginous rod which was longer than that of the fat sand rat. It may be concluded that the histological differences between the tongues of both Crocodylus niloticus and Psammomys obesus, obviously reflect their carnivorous and herbivorous modes of feeding respectively.

Morphology of the dorsal lingual papillae in the blackbuck, Antilope cervicapra.

The dorsal lingual surface of a blackbuck (Antilope cervicapra) was examined by scanning electron microscopy (SEM). The tongue was about 125 mm in length. There were about 30 vallate papillae on both sides. Filiform, conical, fungiform and vallate papillae were found. The filiform papillae were distributed over the entire dorsal surface of the tongue, excepted for the lingual torus where conical papillae were present. The fugiform papillae were present rounded bodies, and more densely distributed on the tip and ventral surface of ligual apex. No foliate papillae were seen on the dorsal surface. The vallate papillae were located on both sides of the midline in the caudal part. Each papilla was surrounded by a groove. These findings indicate that the tongue of the blackbuck is similar to that of the formosan and japanese serow.

SEM study on the dorsal lingual surface of Microtus montebelli.

The dorsal lingual surface of Microtus montebelli was examined by scanning electron microscopy (SEM). The filiform papillae were distributed over the entire dorsal surface of the tongue. The filiform papillae notably differed in morphology by their location on the tongue and could be classified into 4 types: 1) simple conical papillae, 2) true filiform papillae, 3) giant conical papillae, and 4) saw-like papillae. The fugiform papillae were present rounded bodies scattered over the anterior dorsal surface of the tongue. A vallate papilla was located at the middle end of the lingual body. The surface of the radix zone was smooth and without lingual papillae.

Ultrastructure of Weber's salivary glands of the root of the tongue in the rat.

The mammalian tongue encompasses several sizeable agglomerations of minor salivary glands. The ultrastructure of the various glands in the body of that organ has already been determined. In contrast, almost nothing is known of the structure of Weber's glands, a collection of salivary glands in the root of the tongue. The entire tongue was extirpated from anesthetized adult male rats that had been perfused with fixative and specimens of tissue that included Weber's glands were dissected from the lingual root. These were prepared for electron microscopic examination by conventional means. Weber's glands in the rat are mixed glands, consisting of long mucous tubules that often are capped by serous demilunes. There appear to be no ducts per se, the mucous tubules increasing in caliber and approaching the crypts of the dorsal lingual surface while still retaining their mucous secretory character. As these ducts near the surface, their lining changes to stratified squamous epithelium. The mucous cells are cytologically similar to those in the sublingual glands of the same animal. Mucous droplets undergo vertical fusion to form centrally situated intracellular channels through which mucus is exocytosed, much in the same manner as goblet cells. The serous cells, which have all of the hallmarks of protein-secreting cells, probably empty their secretory granules via cellular extensions that directly reach the tubule lumen since there are no intercellular canaliculi between adjacent mucous cells. The mucus elaborated by Weber's glands undoubtedly aids in swallowing dry food. We postulate that the serous cells in these glands, as in the more anterior von Ebner's glands, might play a role in the mechanism of taste, especially where posteriorly situated, nonlingual taste buds are concerned.

Morphostructural study of the lingual papillae in the buffalo (Bubalus bubalus)

The morphostructural characteristics of lingual papillae in 30 adult buffaloes were studied by SEM. In this species, dorsal lingual surface papillae shows different shape so that their classification is complicated. Topography and morphology of conical and vallate papillae presents significant aspects. Particularly, the conical papillae have a different morphological organization when they are localized over the side of the tongue apex or over dorso-lateral tract of the tongue body. The functional aspects are discussed.

SEM study on the dorsal lingual surface of the common tree shrew, Tupaia glis.

The dorsal lingual surface of the common tree shrew was examined by SEM after treating it with HCl to remove the mucous substance. Filiform (FI), fungiform (FU) and circumvallate papillae (CI) were observed. The FI exhibited a small circular bulge surrounded by anterior and posterior filamentous processes. FU were scattered among the FI. There were 3 CI separating the anterior 4/5 from the posterior 1/5 of the tongue. In addition, a group of conical projections with caudal orientation was found anterior to the palatoglossal fold on each side of the tongue. Microridges were widely observed on the entire dorsal lingual surface, except on the free surface of FI processes.

Fine structure of the dorsal epithelium of the tongue of the Japanese terrapin, Clemmys japonica (Cheloia, Emydinae).

As reptiles are situated phylogenetically between the amphibians and the mammals, they exhibit considerable variation in the structure of their tongues. The present study, one of a series of studies on reptile tongues, aims to demonstrate the three-dimensional structure of the dorsal lingual surface of a turtle, the Japanese terrapin Clemmys japonica, and to clarify the ultrastructural features of the lingual epithelial cells. In the study lingual papillae were observed by scanning electron microscopy to be widely distributed over the dorsal surface of the tongue. Irregularly shaped (conical, columnar or angular) papillae were located in the anterior and central areas, and ridge-like ones, in the latero-posterior area. Histological examination revealed that the connective tissue penetrated into the core of the papillae, and the epithelium was of a stratified squamous and/or cuboidal type. Under the transmission electron microscope, two types of cells were identified in the intermediate layer of the apical epithelium of the lingual papilla: one type was probably an immature mucous cell, whereas the other was elongated in a baso-apical direction, its cytoplasm containing fine granules. In the surface layer of the apical epithelium, typical mucous cells and cells containing numerous, fine, electron-lucent granules were recognized. Both types of cells possessed microvilli on their free-surfaces. In the lateral epithelium of the lingual papillae, the cytological features from the basal layer to the superficial intermediate layer were essentially the same as in the apical epithelium. However, in the surface layer, mucous cells were significantly larger in number than in the apical epithelium.

Examination of the Dorsal Lingual Surface of the Crab-eating Monkey Macaca irus by Scanning Electron Microscope

Examination of the Dorsal Lingual Surface of the Crab-eating Monkey Macaca irus by Scanning Electron Microscope

Morphogenesis of rat lingual filiform papillae.

The morphogenesis of filiform papillae on rat tongue was investigated with the electron microscope. Tongue rudiments were first seen on the 12th day of gestation. At 15-17 days, dermal papillae had formed and were arranged in hexagonal array on the dorsal lingual surface. Capping each dermal papilla was a two-layered epithelium that protruded slightly above the lingual surface, thus forming the early filiform papilla. In the next stage of development, at 18-19 days of gestation, the epithelium lining the papilla had differentiated into two cell populations, one producing hard keratin, the other producing soft keratin. Some of the keratinized epithelial cells assumed a position at an acute angle to the tongue surface and extended deep into the epithelium. In the next stage, 20-21 days, a cleft appeared within these angularly oriented cells. This resulted in the division of the epithelium into keraatin-lined individual filiform papillae. Finally, the individual papillae increased in size to the adult form.