Aims & Objectives: Stroke volume (SV) and, consequently, cardiac output monitoring is a cornerstone of advanced hemodynamic assessment. Non-invasive monitoring technologies are increasingly used in pediatric populations. This study compared SV measurements obtained by transcutaneous Doppler ultrasound techniques (USCOM), transthoracic echocardiography jugular (TTE-J), and apical views (TTE-P) performed by cardiologists and non-cardiologists in pediatric spontaneously ventilating patients. Methods A single center study was conducted on patients aged 7.6 ± 4.65 years. In total, 37 patients were enrolled in the study. USCOM and TTE were used to measure SV. Each operator obtained three sets of USCOM measurements within a period of 3–5 minutes, followed with TTE measurements from both apical and suprasternal views. The USCOM and TTE evaluations were performed by two investigators—operator A, non-cardiologist (OP-A) and operator B, cardiologist (OP-B). Results Both USCOM and TTE methods were applicable in 89% of patients. The intra-observer variability of USCOM measurements were 5.4 (5.6) and 7.9 (8.0) in OP-A and OP-B, respectively. The intra-observer variability of TTE-J and TTE-P measurements were 5.8 (5.4) and 6.2 (4.0) in OP-A and 6.5 (4.3) and 7.8 (5.8) in OP-B. The percentage error in SV with USCOM was 39% relative to TTE-J in OP-A and 46% in OP-B. The percentage error in SV with TTE-P was 33% relative to TTE-J in OP-A and 19% in OP-B. Conclusions USCOM and TTE-J show low intra-operator variability. The methods are not interchangeable as SV values by USCOM are higher in comparison to the SV values obtained by TTE from jugular and apical views.