The antihemophilic factor (A.H.F., Factor VIII) level in the plasma of 221 normal donors has been assayed in an attempt to set up a panel of high level A.H.F. donors for use in the treatment of patients with hemophilia. Fifteen per cent of the donors tested were unacceptable for the panel owing to A.H.F. levels of less than 90 per cent. The validity of the assay method used is discussed. The normal range of A.H.F. activity in a random population has been found to agree with that established by other investigators. The optimal conditions for collection, preparation and storage of fresh frozen plasma in order to minimize the loss of A.H.F. are listed.
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