We study matrix string scattering amplitudes and matrix string instantons on a marked Riemann surface in the limit of a vanishing string coupling constant. We give an explicit parameterization of the moduli space of such instantons. We also give a description of the set of fermionic supermoduli. The integration over the supermoduli leads to the inclusion of picture changing operators at the interaction points. Finally we investigate the large N limit of the measure on the instanton moduli space and show its convergence to the Weil-Petersson measure on the moduli space of marked Riemann surfaces.