Samples of the lone star tick, Amblyomma americanum (L.), were taken systematically in 10 study areas. With each sample, the type of vegetation was ascertained and data recorded included such parameters as the dominant herbaceous and woody species of plants, density and height of vegetation, amount of leaf litter on the ground, and location of the sample area with relation to the woods and prairie. Lone star tick nymphs were most abundant in areas where the dominant trees were sassafras, persimmon, or winged elm, whereas lowest nymphal and adult populations existed in open prairies. The highest number of adults were encountered in openings in woods and in sassafras-persimmon woods. Where herbaceous or brushy vegetation in woodlots was thin or absent (0–25%), high numbers of nymphs existed, while larger populations of adults were found where there was more than 75% coverage. The highest adult populations were located where less than 25% of the ground was covered with leaf litter, while nymphs were more abundant in areas with greater than 25% coverage. Observations on tick distribution in vegetation of various heights revealed that adults and nymphs were most prevalent in brush and low trees. Tick numbers collected per drag generally decreased with an increase in height of woody vegetation. In grassy associations nymphal and adult numbers were higher in taller vegetation. A study of tick distribution in prairies showed higher populations of nymphs and adults in the immediate vicinity of the ecotone. In general, populations decreased with an increase in distance from the ecotone.