Among all types of water pollutants, pesticides are of the greatest concern. Carbosulfan is one such carbamate pesticide extensively employed in rural communities, that enters the aquatic environment by the proximity of agricultural lands to water bodies or through direct application in such environments. The consequences of these sublethal pollutants/toxicants exposures can be quantified in terms of biochemical, physiological, or the fish organism's histological reactions. Proteins have a unique role in cell metabolism and proteomic analysis provides valuable information when variations that occur within the proteome of organisms are compared as a consequence of biological perturbations or external stimuli. The study's goal was to investigate the acute toxicity of carbosulfan and its influence on electrophoretic protein patterns in the tissues of Cyprinus carpio. Fingerlings of C.carpio were subjected to various carbosulfan concentrations ranging from 2.6 mg/L to 4.6 mg/L for 96 h, and acute toxicity was determined as 3.41 mg/L. The comparison of the 96 h LC50 values between fish species shows that C.carpio is more resistant to carbosulfan. SDS-PAGE was performed on muscle, liver, gill, and brain tissues after exposing fish to two sublethal concentrations (1/10th and 1/5th of the 96 h LC50) in a semi-static system for 3 weeks. The intensity of protein subunits in carbosulfan-exposed tissues decreased, and some protein subunits disappeared. Carbosulfan attacks proteins having two or more domains easily in low concentrations, and as concentration increases, it begins to attack single domain proteins as well. Variations in protein subunits band patterns due to protein deprivation, proteolysis, or maybe insufficient amino acid incorporation into the polypeptide chain. The study concludes that carbosulfan provides a larger risk to the animal and alters the entire physiology of the fish. Given the importance of fish in human nutrition, biological monitoring of water and fish meat should be done frequently to ensure the continued safety of freshwater food. To prevent these pesticides and other toxins from entering the environment, safe disposal and recycling of domestic sewage and industrial wastes should be practiced and precautions should be taken while using even low concentrations of carbosulfan, and prohibiting or restricting carbosulfan usage is preferable.