Domestic Genetic Resources Research Articles

Genetic features of representatives of Malus Mill. for the formation of identified collections and breeding use

For the active mobilization of bioresources of the leading agricultural fruit plants, especially apple (Malus × domestica Borkh.), and the inclusion of the best samples of the gene pool in the breeding, it is necessary to know their genetic characteristics according to the priority desired traits. The purpose of the study is to study the allelic polymorphism of the Md-PG1 in representatives of Malus Mill. to identify sources of long-term keeping of fruits and to form an identified collection of apple trees for breeding use. The studies were carried out in the Center for Collective Use «Research and Breeding Collection of Genetic Resources of Horticultural Crops»; generally accepted programs and methods of variety study and breeding of apple trees. Based on the data of molecular genetic analysis, carriers of the allelic combination AA for the Md-PG1 gene were identified - apple varieties: Smeralda, Egemen, Georgiya, hybrids 17/1-6-42 (Champion × Modi) and 17/2-5-15 (Renuartsiv × Carmen). The selected varieties and hybrid forms are promising in the process of creating domestic genetic resources with improved quality indicators, including a genetically determined increase in the shelf life of fruits.

Economic principles of innovative development of breeding pig breeding in Ukraine

The article is devoted to the economic principles of formation of innovative development of breeding pig breeding in Ukraine. It is established that the breeding herd of breeding plants is carried out on the principle of self-repair, breeding pigs by the method of "closed" population, breeding breeders - mainly repair pigs of own reproduction, obtained and raised directly on the farm. Repair boars to replenish the herd are purchased from other farms, and when the ancestral herd is organized, they are raised directly in the association. In commodity herds of pig farms and breeding complexes it is desirable to carry out repair boars and pigs from breeding farms, and if necessary with a clear primary zootechnical record breeding repair pigs to replace lost queens can be carried out directly in the enterprise. Given the limited import of breeding pigs due to the spread of ASF in the world, the main task of breeding farms is the cultivation and sale of high quality young stock. However, in recent years, repair boars and pigs obtained and raised in domestic breeding enterprises are often not in demand in the market. Based on the results of the analysis, it can be concluded that the established domestic breeding base does not require unsystematic mass imports except for a small number of high-value breeding young or sperm odes of prominent breeding boars to refresh the blood of some herds under the methodical control provided by the state scientific and technical programs. The current situation in industrial pig breeding primarily requires solving the problems of automation of management of breeding and breeding work with the use of modern computer technology. The solution of this problem is especially important if the production of commercial pork uses hybridization systems, which requires a clear organization of the breeding herd when working with large populations of animals. At the same time, stabilization and further effective development of breeding enterprises cannot be ensured without modernization of instruments of state support of breeding farms, preservation and efficient use of the best domestic genetic resources and world gene pool of pig breeds, improvement of production activities and strengthening of integration with large industrial complexes. Key words: breeding pig breeding, agricultural enterprise, state support, commercial pig breeding, innovation.

Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds

Karakaş and Norduz sheep have been adapted to the Lake Van region of Turkey for many years as being domestic genetic resources and meat, milk, and fleece traits are a reliable economic resource especially preferred by small family businesses around the area. In this study, the data set consisted of milk yield (MY) and milk content components; milk fat (MF), fat-free dry matter (FFDM), dry matter (DM), protein (Pro), lactose (Lac), pH, acidity H (aH), and lactic acid (Lac). Besides, Karakaş (n = 30) and Norduz (n = 26) sheep were chosen to investigate based on the SNP method. Correspondingly, genomic DNA from both breeds exon 2 of the GDF9 gene region was amplified, 815 base pairs (bp) in length, by means of PCR. Therefore, there were three noval SNPs detected in both breeds under investigation. Although SNP1 and 2 with genotypes of GG and AG had statistically significant impacts on both milk production and milk components (p < 0.01), SNP3 with genotypes of TT, TC had no significant effects on the milk characteristics in question.

Mobilization of genetic diversity of the genus Malus on the basis of information technologies for the breeding of varieties with complex resistance to fungal pathogens

Breeding renewal of the southern assortment of apple trees for the Russian fruit growing industry based on the accelerated process of creating domestic genotypes with a complex increased resistance to the main fungal pathogens of the culture (Venturia inaequalis (Cooke) Wint., Podosphaera leucotricha (Ell. &amp; Ev.) E.S. Salmon, Phyllosticta mali Pr. et. Del., Phyllosticta briardi Sacc.) is due to the systematic use of modern methods, innovative breeding techniques and improved techniques. The purpose of the study is to develop and create a database of representatives of the genus Malus Mill., which is promising for use in the production and accelerated breeding of new domestic genetic resources for the southern region of Russia. During the research work, Russian modern and generally accepted programs and methods of breeding, variety study for fruit plants were used. The formed database “Main economically valuable, morphological and molecular-genetic characters of representatives of the genus Malus Mill. with resistance to the main fungal pathogens in the south of Russia”; filed an application for its patent. Using the data of the obtained information system will significantly speed up the solution of practical selection problems and reduce the costs of organizing the breeding process.

Contemporary evolution of maize landraces and their wild relatives influenced by gene flow with modern maize varieties

Mexico is recognized as the center of origin and domestication of maize. Introduction of modern maize varieties (MVs) into Mexico raised concerns regarding the possible effects of gene flow from MVs into maize landraces (LRs) and their wild relatives (WRs), teosintes. However, after more than 60 y from the release of the first MVs, the impact of the sympatry with LRs and their WRs has not been explored with genetic data. In this work, we assessed changes in the genomes of 7 maize LRs and 2 WR subspecies from collections spanning over 70 y. We compared the genotypes obtained by genotyping by sequencing (GBS) for LRs and WRs before and after the adoption of MVs, and observed introgression from sympatric MVs into LRs and into the WR Zea mays ssp. mexicana sampled after the year 2000. We also found a decrease in the paired divergence index (FST ) between MV-LR and MV-WR over the same time frame. Moreover, we determined that LR genetic diversity increased after 2000, probably as a result of gene flow from MVs introduced in the 1990s. Our findings allowed us to identify ongoing changes in the domesticated and wild maize genetic pools, and concur with previous works that have evaluated short-term gene flow from MVs into LRs in other crops. Our approach represents a useful tool for tracking evolutionary change in wild and domesticated genetic resources, as well as for developing strategies for their conservation.

Screening of cryoprotectants (CPAs) for cryopreservation in the Nitzschia sp. of marine microalgae

Biodiversity has continued to degrade in the 21st century due to global warming occasioned by destruction of the environment around the world.. The Nagoya protocol places Korea in a unique position to effectively develop and protect its domestic genetic resources. Microalgae under study in this research contains large amount of antioxidant substances such as beta carotene and astaxanthin, that can be used as biological resource owing to the large amounts of biomass that can be secured through photosynthesis. However, it is difficult to preserve it since cryopreservation method used for long-term preservation is yet to be developed. A basic study for long term cryopreservation was carried out on Nizschia frustulum and Nitzschia amabilis which belong to marine diatoms. As cryoprotectants (CPAs), glycerol, DMSO, and methanol which penetrate into cells were prepared at 5%, 10%, and 15% concentrations each, in case of methanol, it was tested at concentrations of 5%, 10% and 12% by its nature. Two kinds of microalgae, N. frustulum and N. amabilis, were diluted with 102, 103 and 104 cells ml-1, respectively. The highest survival rate was shown at12% concentration of methanol, and the figures were 6.94 ± 0.31% in N. frustulum and 8.85 ± 0.16% in N. amabilis. As a result of 3 weeks cultivation of thawed microalgae after freezing, the result is shows that N. frustulum increased about 10 times faster and N. amabilis increased about 12 times the original concentration.

RATIONAL MANAGEMENT OF THE GENE POOL OF VALUABLE BREEDS OF ANIMALS FOR THE PURPOSE OF CONSERVATION OF BIOLOGICAL DIVERSITY

Aim. The aim is to conduct an analysis of biodiversity of valuable breeds of animals, as well as to assess its condition, find ways of preserving domestic genetic resources and identify their economically valuable qualities. Discussion. The material is presented, which proves the need to preserve valuable, rare and endangered breeds of agricultural animals characterized by high quality indices of production, longevity, fertility, resistance to diseases, adaptive abilities to extreme environmental conditions. Importance of carrying out these measures is associated with the absorption of domestic flocks by foreign breeds, which led to a decrease in genetic diversity and the continuing trend in the displacement of rare and endangered breeds of animals. One of the ways of preserving animals that cause breeders' concern is the use of seed cryopreservation, which is actively pursued by a number of scientists and specialized institutions. Lack of effective legislative framework, as well as the lack of state support for conservation programs for these breeds, causes significant damage to the problem of preserving local breeds. Together with the listed benefits from breeding local livestock, their role in the biological diversity should be taken into account. Conclusion. Despite the invaluable contribution to the preservation of local breeds and animal populations made by the profile research institutes (the Federal Research Center for Animal Husbandry - L. K. Ernst Institute of Animal Husbandry, All Russian Research Institute Of Animal Breeding) and individual scientists, the given problem requires its inclusion in the legislative framework and effective state support.

A review of genetic resources of almonds and stone fruits (Prunus spp.) in Iran

Iran is amongst the countries in the world widely known for cultivation of Prunus spp. (or stone fruits). It is both a centre of origin and diversity of the stone fruits. Numerous wild species as well as many cultivars and landraces of these fruit crops are important genetic resources today in Iran and can be used for improvement and breeding of scion and rootstock cultivars which are resistant to many biotic and abiotic stresses through modern genomics and genetic technologies. This paper discusses the distribution, ethno-botany, diversity and utilization of wild and domesticated genetic resources of stone fruits including almond (Prunus dulcis (Miller) D. A. Webb.), peach and nectarine (P. persica Batsch), European and Japanese plum (P. × domestica L., and P. salicina L., respectively), sweet and sour cherry (P. avium L., and P. cerasus L., respectively), and apricot (P. armeniaca L.), all of which are members of the Rosaceae family. The goal of this paper is to highlight the importance of Iran as a main contributor to the diversity of Prunus genetic resources in the world, as well as, present major achievements regarding identification, collection, evaluation, conservation and utilization of this valuable genetic resource in Iran.

A review of genetic resources of pome fruits in Iran

Iran is very rich in plant genetic resources, due largely to the diversity in the agro-ecological landscape. Protection, preservation and utilization of genetic resources would facilitate development of premium varieties and biotech products that can potentially lead to a change in the food, agricultural, pharmaceutical and chemical industries in Iran. This paper discusses the distribution, ethnobotany, diversity and utilization of wild and domestic genetic resources of pome fruit species in Iran including apple (Malus spp.), pear (Pyrus spp.), quince (Cydonia oblonga Mill.), hawthorn (Crataegus spp.), medlar (Mespilus germanica L.) and loquat (Eriobotrya japonica Lindl.), all of which are members of the Rosaceae family. The goal of this paper is to highlight the importance of Iran as a contributor to the diversity of pome fruit genetic resources in the world.

Identification of facultative heterofermentative Lactobacillus species in Lighvan cheese.

Facultative Heterofermentative Lactobacilli (FHL) are responsible for enhancing unique flavour properties in traditional dairy products such as Lighvan cheese through biochemical mechanisms. In addition to saving domestic genetic resources, industrial production of semi-traditional dairy products is one of the major objectives of non starter microflora identification in indigenous dairy products. In this study performed on the Lighvan cheese at 4 months ripening period Based on morphologicaland biochemical methods were identified Lb. plantarum group (Lb.pentosus, Lb. plantarum, Lb. paraplantarum) and Lb. casei group ( Lb. casei, Lb. paracasei) .Moreove the development of the FHL in Lighvan cheese varied according to ripening time. Keywords: Lighvan cheese, Lactobacillus plantarum, Lactobacillus casei

Changes in selected physicochemical parameters of breast muscles of geese from Polish conservation flocks depending on duration of the post slaughter period

Abstract. In four Polish conservative flocks of Suwałki (Su), Kartuzy (Ka), Kielce (Ki) and Subcarpathian (Sb) geese, registered by FAO as domestic genetic resources the mean values of qualitative traits of breast muscles (pH15', pH30', pH45', pH24h; colour parameters: L*15', L*30', L*45', L24h, a*15', a*30', a*45', a*24h, b*15', b*30', b*45', b*24h and conductivity: EC45', EC3h, EC24h) were determined. The experimental flocks showed significant variations in the investigated parameters. The Kartuzy goose breast muscles were characterized by higher values of pH15' (6.74), pH30' (6.62), pH45' (6.56) and b*15' parameter (0.80) in comparison with the remaining flocks. Moreover, these muscles had the lowest pH24h (5.65) and values of L* parameter (L*15', L*30', L*45' and L*24h) and EC45' (3.23 mS/cm). The pH value and L* and a* parameters (except L*24h and a*24h) of the analysed flocks were decreasing, and conductivity increasing together with the time post slaughter, and the rate of changes depended on the birds’ origin. Greater differences between pH values, measured 15 min and 24 h after slaughter were stated in breast muscles from Ka (1.09) and Su (0.90) geese than from the other two flocks investigated (Ki – 0.74; Sb – 0.58). The highest increase in conductivity (from EC45' to EC24h) was affirmed in Ka and Ki muscles (3.84 mS/cm) and the lowest in breast muscles of Sb geese (2.72 mS/cm).

Comparison of physical and qualitative traits of meat of two Polish conservative flocks of ducks

Abstract. In two Polish conservative flocks of Miniduck (K2) and Pekin (P33), registered by FAO as domestic genetic resources (World Watch List, 2000) the mean values of physical (tissue components) and qualitative traits of meat (pH15, pH24, colour of meat) were determined. The experimental flocks showed significant variations in body weight at 7 weeks of age (1540 vs 2088 in drakes and 1395 vs 1986 g in ducks), in the proportion of breast and leg muscles and skin with subcutaneous fat in eviscerated carcass. K2 ducks are characterized by outstanding musculature of breast and P33 ducks by that of lower leg and by low fatness. pH15 values of breast and leg muscles in K2 and P33 birds were greater than pH24 of these muscles. In both flocks pH15 values of 6.09 to 6.33 and pH24 values of 5.90 to 6.05 in breast muscles were lower than in leg muscles (6.33 to 6.46 and 6.12 to 6.37, respectively). A significantly lower L* value (darker colour) of breast muscle was characteristic of K2 birds (43.7 in males and 40.5 in females) compared to P33 males and females. Furthermore, breast muscles of K2 drakes and ducks had significantly higher redness (a*) and yellowness values (b*) than P33. In pectoralis superficialis muscle of K2 and P33 females, a significantly lower diameter was found for white muscle fibres (αW) (30.0 to 30.5 μm) and for red muscle fibres (βR) (16.9 to 17.6 μm) than in biceps femoris muscle (αW from 48.3 to 54.2; βR from 36.0 to 37.1 μm). Muscle fibres of K2 compared to P33 ducks were characterized by significantly greater diameters of βR muscle fibre in pectoralis superficialis muscle, and by lower αW and βR fibre diameters in biceps femoris muscle. The experiment showed that meat of ducks from the conservative flocks studied is valuable from the consumer’s point of view.