Domestic Animals Research Articles

Bioactive Molecules, Ethnomedicinal Uses, Toxicology, and Pharmacology of Peltophorum africanum Sond (Fabaceae): Systematic Review

Plants have long been used to treat serious illnesses in both humans and animals. A significant underappreciated medicinal tree, Peltophorum africanum Sond is utilized by many different ethnic groups to cure a wide range of illnesses. A variety of electronic databases, including ScienceDirect, Scopus, Scielo, Scifinder, PubMed, Web of Science, Medline, and Google Scholar, were used to search the literature on P. africanum, using key words such as uses, survey, pharmacology, antigonococcal, toxicity, phytochemistry and others. Further data was obtained from several scholarly theses, dissertations, and books on general plant sciences, ethnomedicine, and other pertinent ethnobotanical topics. The plant species possess very important pharmacological activities in vitro, which includes antimicrobial, anti-HIV, antioxidant, anticancer, antidiabetic, and other activities. Phytochemically, the plant possesses various classes of compounds, dominated by flavonols, which may well explain its wider range of pharmacological activities. Although the plant is promising anti-HIV activity, the mode of action and safety profiles of the plant also need to be explored as its extracts exerted some degree of mutagenicity. It is also important to further explore its ethnoveterinary use against a plethora of nematodes that infects both wild and domestic animals. Given its potent pharmacological activity, the further in vivo studies need to be explored to ascertain the comprehensive toxicology of the plant species, thereby developing possible medications. The plant species may further be elevated to a potent pharmaceutical product against plethora of infections.

Morphological and Molecular Identification of Obligatory Myiasis-Causing Species in Wild Cervids in Croatia

The infestation of ruminants by fly larvae of the Oestridae family can lead to nasopharyngeal or subcutaneous myiasis, which can harm animal welfare and productivity on sheep and cattle farms. The infestation of wild ruminants allows them to serve as reservoirs for subsequent transfer to domestic animals. The fly species most commonly responsible for myiasis have been well studied in many parts of the world, but less so in southeastern Europe. Here, we identified three botfly species among 197 subcutaneous larvae and 138 nasopharyngeal larvae from 36 wild roe deer (Capreolus capreolus) and 9 wild red deer (Cervus elaphus) at 30 locations across Croatia. Instars were stored in 75% ethanol and their species and developmental stage were identified based on morphological and morphometric keys. Subcutaneous larvae in both cervid species were identified as Hypoderma diana, while nasopharyngeal larvae were identified as Cephenemyia simulator in roe deer and Pharyngomyia picta in red deer. Partial sequencing of the gene-encoding mitochondrial cytochrome oxidase subunit I confirmed the taxonomic classification of C. stimulator and P. picta but was unable to confirm that of H. diana for lack of previously published sequences. This study appears to be the first to combine the morphological and molecular characterization of myiasis-causing botflies in wild cervids in southeastern Europe.

Molecular Characterization, Oxidative Stress-Mediated Genotoxicity, and Hemato-Biochemical Changes in Domestic Water Buffaloes Naturally Infected with Trypanosoma evansi Under Field Conditions

(1) Background: Surra is a debilitating disease of wild and domestic animals caused by Trypanosoma evansi (T. evansi), resulting in significant mortality and production losses in the affected animals. This study is the first to assess the genetic relationships of T. evansi in naturally affected buffaloes from Multan district, Pakistan, using ITS-1 primers and evaluating the effects of parasitemia and oxidative stress on DNA damage and hematobiochemical changes in infected buffaloes. (2) Methods: Blood samples were collected from 167 buffaloes using a multi-stage cluster sampling strategy, and trypomastigote identification was performed through microscopy and PCR targeting RoTat 1.2 and ITS-1 primers. Molecular characterization involved ITS-1 via neighbor-joining analysis. The impact of parasitemia loads was correlated with oxidative stress markers, genotoxicity, and hematobiochemical parameters using Pearson correlation and multivariable regression models. (3) Results: Field-stained thin blood film microscopy and molecular identification revealed 8.98% and 10.18% infection rates, respectively. Phylogenetic analysis based on ITS-1 region sequences of the identified isolates showed close genetic associations with Indian isolates. The mean trypomastigote count observed in the infected buffaloes was 5.15 × 106 (±5.3 × 102)/µL of blood. The parasitemia loads were significantly correlated with the alterations in oxidative stress markers, DNA damage, and changes in hematobiochemical parameters. Infected animals exhibited significant (p < 0.05) alterations in oxidative stress biomarkers, including catalase, nitric oxide, and malondialdehyde concentrations. Noteworthily, a comet assay revealed a significantly (p < 0.0001) higher mean genetic damage index in the infected buffaloes (0.7 ± 0.04) compared with the healthy ones (0.196 ± 0.004). Alongside significant (p < 0.05) reductions in red cell indices, a marked elevation in leukocyte counts and serum hepatic enzyme levels was recorded in the affected buffaloes. (4) Conclusion: T. evansi isolates of buffaloes from Multan, Pakistan, have genetic similarities to Indian isolates. This study also revealed that higher parasitemia loads induce genotoxicity in the infected animals through oxidative stress and cause hematobiochemical alterations under natural field conditions.

Rabies transmitted from vampires to cattle: An overview

Rabies is a zoonotic infectious disease of global distribution that impacts human and animal health. In rural Latin America, rabies negatively impacts food security and the economy due to losses in livestock production. The common vampire bat, Desmodus rotundus, is the main reservoir and transmitter of rabies virus (RABV) to domestic animals in Latin America. Desmodus rotundus RABV is known to impact the cattle industry, from small farmers to large corporations. We assessed the main patterns of rabies in cattle attributed to D. rotundus RABV across Latin America. Epidemiological data on rabies from Latin America were collected from the Pan American Health Organization spanning the 1970–2023 period. Analyses revealed an average of 450 outbreaks annually for the countries where D. rotundus is distributed, with at least 6 animals dying in each outbreak. Brazil, Colombia, Peru, and Mexico were the Latin American countries with the highest number of rabies outbreaks during the study period and are the most affected countries in recent years. Findings suggest a re-emergence of bat-borne rabies in the region with more outbreaks reported in recent years, especially during the 2003–2020 period. Rabies outbreaks in cattle in the 2000–2020 period were significantly more frequent than in previous decades, with an increase in cross-species transmission after 2002. The size of outbreaks, however, was smaller in recent years, involving lower cattle mortality. Peru, El Salvador, and Brazil showed a strong association (R = 0.73, p = 0.01) between rabies incidence in D. rotundus (rates per million humans: 1.61, 0.94, and 1.09, respectively) and rabies outbreaks in cattle (rates per million cattle: 465.85, 351.01, and 48.22, respectively). A sustained, standardized, and widespread monitoring of D. rotundus demography and health could serve to inform an early warning system for the early detection of RABV and other bat-borne pathogens in Latin America. Current data can be used to forecast when, where, and in which intensity RABV outbreaks are more likely to occur in subtropical and tropical Latin America. A decrease in the size of outbreaks could suggest that strategies for epidemic management (e.g., education, early diagnosis, vaccination) have been effective. The increase in the number of outbreaks could suggest that the factors facilitating cross-species transmission could be on the rise.

The fabric of zoodemocracy: a systemic approach to deliberative zoodemocracy

ABSTRACT In this article, I explore whether domesticated animals (DAs) of different species belonging to the same community participate in authoring and sustaining, what I call, the fabric of zoodemocracy. The fabric refers to a set of activities, social norms, and values that together sustain our democracies (e.g. cooperation, protest, and helping one’s neighbour). I explore this by situating my intervention within systemic theories of democracy and the political turn in animal rights theory. Specifically, I situate my work within Donaldson and Kymlicka’s zoopolitical project, arguing that animals are owed rights to participation in decision-making as community members. Some, however, challenge the idea that DAs can actually sustain democratic communities because they do not have, for example, the rational capacities to control themselves. Others suggest that there are conceptual and normative problems with the concept of co-authorship advanced by Donaldson and Kymlicka. This article addresses these debates by (1) identifying some of the key elements needed to sustain the fabric of democratic communities; and (2) empirically testing whether DAs have the necessary capacities to sustain such communities. I do this by analysing the data I collected while conducting a multispecies ethnography at VINE sanctuary, Springfield, Vermont, US, for 8 weeks.*

Leptospira biofilms: implications for survival, transmission, and disease management.

Leptospirosis is a zoonotic disease caused by Leptospira bacteria, affecting humans and a broad range of wild and domestic animals in diverse epidemiological settings (rural, urban, and wild). The disease's pathogenesis and epidemiology are complex networks not fully elucidated. Epidemiology reflects the One Health integrated approach of environment-animal-human interaction, causing severe illness in humans and animals, with consequent public health burdens. Saprophytic and pathogenic leptospires have been shown to form biofilms in vivo, in vitro, and in environmental samples. Biofilms are characterized by a polymeric matrix that confers protection against hostile environments (both inside and outside of the host), favoring bacterial survival and dissemination. Despite its significance, the role of this bacterial growth mode in leptospiral survival, transmission, and decreased antibiotic susceptibility remains poorly understood and underexplored. Even so, the literature indicates that biofilms might be correlated with lower antimicrobial susceptibility and chronicity in leptospirosis. In this minireview, we discuss the aspects of biofilm formation by Leptospira and their significance for epidemiology and therapeutic management. Understanding the current scenario provides insight into the future prospects for biofilm diagnosis, prevention, and treatment of leptospirosis.

Molecular Detection of Coxiella burnetii in Ticks Isolated from Domestic Animals in Slaughterhouses and Farms, Shahr-E-Rey, Tehran, Iran

Background: Coxiella burnetii causes Q fever, a zoonotic and vector-borne disease. Ticks serve as vectors for this bac­terium. This study aimed to determine the prevalence of C. burnetii infection in ticks in Shahr-e-Rey County, Tehran Province. Methods: From December 2016 to November 2017, 179 ticks were collected on sheep at animal husbandry facilities and slaughterhouses located in Shahr-e-Rey, Tehran Province. Tick samples were morphologically identified and evalu­ated for the presence of the C. burnetii IS1111 gene using real-time PCR. Results: Ticks were classified into four genera: Hyalomma (66.48%), Rhipicepalus (23.47%), Dermacentor (7.26%), and Ornithodoros (2.79%). Furthermore, 35.20% of the ticks were Hyalomma nymphs. All 77 ticks were pooled by species, and C. burnetii was found in 22.08% (n= 17). Ornithodoros lahorensis was the most prevalent tick infected with C. burnetii. Conclusion: The distribution of C. burnetii and reports of Q fever from various regions of the country strongly suggest that the monitoring system should give this disease more attention.

Absence of infection by Trichinella spp. (Nematoda: Trichinellidae) in free-living wild carnivores in Brazil

BackgroundNematodes of the genus Trichinella are foodborne zoonotic pathogens that are widespread globally. These parasites have two epidemiological cycles, domestic and sylvatic, with the latter having wild carnivores as the main reservoirs of the parasite. Trichinella spp. have been increasingly detected in wild carnivores in Argentina and Chile. Although the disease is absent in domestic animals in Brazil, there is serological evidence that the agent is circulating in wild boars in some areas. This study aimed to diagnose Trichinella spp. infection through artificial tissue digestion and histopathology of selected tissues of wild carnivores from São Paulo state, southeastern Brazil.MethodsTissue samples (forearm muscles, diaphragm, and tongue) from 53 wild carnivores (21 Canidae, 25 Felidae, 04 Mustelidae, 03 Procyonidae) were used, along with a retrospective study of the slide bank, considering samples from the period 2010 to 2021, totaling 89 free-living carnivores (42 Canidae, 42 Felidae, 03 Mustelidae, 02 Procyonidae).ResultsEither artificial digestion or histopathological analyses did not reveal any larvae suggestive of Trichinella spp., indicating that the nematode was not circulating within the target population.ConclusionTo date, there is no direct evidence of nematode circulation in wild carnivores in the study area.

SARS-CoV-2 surveillance and detection in wild, captive, and domesticated animals in Nebraska: 2021–2023

Widespread surveillance for SARS-CoV-2 was conducted across wildlife, captive animals in zoological collections, and domestic cats in Nebraska from 2021 to 2023. The goal of this effort was to determine the prevalence, phylogenetic and spatial distribution characteristics of circulating SARS-CoV-2 variants using various diagnostic methodologies that can utilize both antemortem and postmortem samples, which may be required for wildlife such as white-tailed deer. Statewide surveillance testing revealed high variation in SARS-CoV-2 prevalence among species, with white-tailed deer identified as the primary reservoir. In 2021, seroprevalence in white-tailed deer was 63.73% (n = 91) and 39.66% (n = 237) in 2022, while virus detection in retropharyngeal lymph nodes (RLN) was 16.35% (n = 483) in 2021 and 3.61% (n = 277) in 2022. Phylogenetic analysis was conducted on 11 positive samples from 2021. This analysis revealed the presence of four lineages of the Delta variant: AY.100, AY.119, AY.3, and AY.46.4. Conversely, other species showed no virus detection, except domestic cats, which had a low seroprevalence of 2.38% (n = 628) in 2022, indicating minimal exposure. The detection of SARS-CoV-2 in white-tailed deer and the identification of multiple Delta lineages underscores the need for ongoing surveillance and the importance of using different diagnostic methodologies. These efforts are critical for understanding virus circulation and evolution in wildlife and domestic animals, informing public health strategies, and mitigating the risks of zoonotic transmission of SARS-CoV-2 and other emerging infectious diseases.

First documentation of a jaguar (Panthera onca) with otitis caused by Otobius megnini (Dugés 1883).

Otobius megnini (spinose ear tick) is a cosmopolitan soft tick that parasitizes domestic and wild mammals, as well as humans. The larval and nymphal stages are common parasites that feed on blood inside the canal ears of hosts, while adults are nonfeeding and live off the host. Different nymphal stages of O. megnini were collected from a jaguar (Panthera onca) suffering from otitis in captive conditions from Guerrero, Mexico. This is the first documentation worldwide of O. megnini in a jaguar with otitis. The presence of this tick species represents a risk of transmission to animals and humans exposed to the nymphal stages of the tick.

Avian influenza overview September-December 2024.

Between 21 September and 6 December 2024, 657 highly pathogenic avian influenza (HPAI) A(H5N1) and A(H5N5) virus detections were reported in domestic (341) and wild (316) birds across 27 countries in Europe. Many HPAI outbreaks in domestic birds were clustered in areas with high poultry density and characterised by secondary farm-to-farm spread. Waterfowl, particularly the mute swan, were primarily affected during this reporting period, with HPAI virus detections focused on south-eastern Europe. Notably, A(H5N5) viruses expanded their geographic and host range, resulting in a surge in detections and mortality events described in gulls and crows. No new HPAI virus detections in mammals were reported in Europe during this reporting period, but the number of dairy cattle farms reportedly affected in the United States of America (USA) rose to >800 in 16 States, and HPAI virus was identified in two pigs in a mixed-species farm. Between 21 September and 11 December 2024, 56 new human cases with avian influenza virus infection were reported from North America (45 A(H5N1) cases), Viet Nam (one A(H5)) and China (ten A(H9N2) cases). Most of the A(H5) human cases in North America (95.6%, n = 43/45) had reported exposure to poultry, live poultry markets, or dairy cattle prior to avian influenza virus detection or onset of illness. Human infections with avian influenza viruses remain rare and no evidence of human-to-human transmission has been documented in the reporting period. The risk of infection with currently circulating avian A(H5) influenza viruses of clade 2.3.4.4b in Europe remains low for the general public in the European Union/European Economic Area (EU/EEA). The risk of infection remains low-to-moderate for those occupationally or otherwise exposed to infected animals or contaminated environments.

Uso de óleos vegetais para melhoria da resistência da madeira ao ataque de térmitas xilófagas

ABSTRACT There are various compounds to increase the natural resistance of wood, but they can be harmful to humans, domestic animals and the environment. Natural products are therefore being researched to ensure the sustainability of the environment, human health and reduce the use of traditional products. The objective of this research was to evaluate the efficiency of andiroba (Carapa guianenses), copaiba (Copaifera spp.) and jatropha (Jatropha curcas) oils in the biological resistance of Pinus elliottii wood to arboreal termites (Nasutitermes corniger). The andiroba and copaiba oils came from communities in the municipality of Santarém, Pará, and the jatropha oil from Fazenda Tamanduá, in the municipality of Santa Terezinha, Paraíba. They were used pure and enriched with sublimated iodine (1, 3, and 5% concentration). The effects of volatilization and leaching on the efficiency of the solutions against Nasutitermes corniger were evaluated. The lowest mass losses and damages were for wood impregnated with copaiba oil, both pure and enriched with iodine. The samples subjected to leaching showed the greatest damage (score = 9.33). Termite mortality was 100% at the end of the assay for all the treatments tested. Copaiba oil can be an environmentally friendly alternative to protect wood, especially wood in direct contact with humans and domestic animals and exposed to environments where Nasutitermes corniger is likely to attack, as it has the lowest mass losses (7.51-6.14%). However, it is not exposed to situations that could cause leaching.

Epidemiological analysis and potential factors affecting the 2022-23 Crimean-Congo hemorrhagic fever outbreak in Iraq.

Crimean-Congo hemorrhagic fever (CCHF) is an acute tick-borne disease with a case fatality rate of up to 40% in humans, posing a significant health threat. This study investigates the 2022-23 CCHF outbreaks in Iraq, the highest recorded to date, and analyzes potential factors at the human-animal-environmental interface. Data from the Iraqi government, the World Health Organization, and the World Bank were used to analyze CCHF trends and affecting factors. This included epidemiological reports, clinical data, tick infestation and seroprevalence studies, and climate data. Descriptive and statistical analyses examined case trends, geographic and demographic characteristics, clinical manifestations, risk factors, seasonal patterns, and influencing factors. A sudden rise in CCHF cases began in southern Iraq in April 2022 and expanded across all governorates, with a shift toward urban areas. Higher incidence was observed among males, aged 25-44, and those involved in slaughtering. The most common clinical manifestation was fever (97%), followed by hemorrhagic symptoms (54%). Bleeding from the gums or mouth and subcutaneous bleeding were more frequent in patients with fatal outcomes. Seasonal patterns showed peaks during spring and fall, correlating with tick activity and potentially exacerbated by climate change. Tick infestation and seroprevalence studies indicated a high prevalence of Hyalomma ticks and CCHF seropositivity among domestic animals in southern Iraq (60%), consistent with the distribution of CCHF human cases. Iraq's ongoing CCHF outbreak demands multidisciplinary One Health strategies. The Iraqi government has adopted such a control strategy, contributing to regional and global efforts to enhance pandemic preparedness.

Serological evidence of Crimean-Congo haemorrhagic fever in domestic animals from eight regions of Namibia.

Crimean-Congo haemorrhagic fever (CCHF) is a viral zoonotic disease endemic to regions of Africa, the Balkans, the Middle East, and Asia, with increasing reports of cases in southern Europe. Human transmission occurs primarily through the bite of infected ticks and by body fluids from infected human. Crimean-Congo haemorrhagic fever virus (CCHFV) affects a broad host range, including both domestic and wild vertebrates. Recent studies have suggested a potential susceptibility of companion animals, posing an additional threat to public health. In the present study, the presence of CCHFV antibodies was evaluated by screening 374 dog and 238 cat serum samples collected from eight Namibian regions using a commercial enzyme-linked immunosorbent assay. Overall, dogs (11.50%) showed significantly higher odds of seropositivity (OR = 7.60; 95% CI = 3.02 - 25.51; p-value < 0.001) compared to cats (1.68%). Most of the positive dogs and all positive cats originated from the Kunene and Hardap regions. No other factors were found to affect seroprevalence. The limited development of farming systems in these regions, combined with the higher roaming activity of dogs, may suggest a greater exposure risk to wild animals or infected ticks. Tick species of the genus Hyalomma are not commonly reported to infest dogs in Namibia, and other tick species may also be involved in transmission. The detection of CCHFV antibodies in dogs and cats in Namibia should be considered by public health authorities as a potential threat, warranting further investigation to identify infection sources and risk factors. While the level and duration of viremia in companion animals, as well as their actual infectivity, remain unknown, efforts should focus on reducing contact between domestic pets, livestock, and wild animals, as well as regular prophylactic tick treatment of pets to prevent or minimise tick infestations.

Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas.

Knowledge of pathogen epidemiological dynamics and habitat ecological features is essential for wildlife population and health monitoring and management. Toxoplasma gondii and Neospora caninum are two broadly distributed multi-host parasites that affect both wild and domestic animals and, in the case of T. gondii, cause zoonosis. This study reports the seroprevalence of both parasites in Iberian ibex (Capra pyrenaica), a mountain wild ruminant native to the Iberian Peninsula, from the Natural Space of Sierra Nevada (NSSN) in southeastern Spain. Serum from 146 Iberian ibexes were analysed using two in-house ELISA techniques. The positive and doubtful sera were further checked by Western Blot (WB). Seventeen ibexes (11.6%; 95% confidence interval 6.4-16.7) were positive for T. gondii and seven (4.8%; 95% confidence interval 1.3-8.2) for N. caninum. However, no sera were positive to T. gondii nor to N. caninum by WB. Using at least two different serological techniques is recommended when they are not validated for the target host species. The NSSN is a hypoendemic area for T. gondii and N. caninum, probably determined by the reduced abundance and restricted distribution of their definitive hosts. This would explain the hypoendemic situation in the NSSN and the lack of specific antibodies against these two parasites in the Iberian ibex population. This eco-epidemiological scenario can be challenged by climate and anthropogenic changes, recommending long-term monitoring Iberian ibex population and health, both as a conservation measure for the species and as an indicator of the potential impact of global change on high mountain ecosystems.

Respiratory System of the Two-Toed Sloth (Choloepus didactylus): Macroscopic, Histological and Scanning Electron Microscopy Analysis.

Relatively little research has been conducted regarding Choloepus didactylus and studies that have been done have mainly covered physiology and morphology, as well as studies on its respiratory system. The objective of this study was to describe the components of the respiratory system of the two-toed sloth and compare it with other species already described in the literature. For this purpose, eight adult animals were used. Macroscopic analyses were carried out on the organs that make up the respiratory system, which confirmed the existence of four cartilages of the larynx, trachea with an average of 37 incomplete tracheal cartilages and lung, highlighting the absence of the accessory pulmonary lobe being composed only of the right cranial and caudal, left cranial and caudal lobes. After the standard protocol, microscopic analyses of fragments of the organs evidenced in the macroscopic study were carried out, using optical microscopy and scanning electron microscopy, in addition to chest x-rays with positive contrast media through orotracheal intubation. Finally, we conclude that microscopically the respiratory system of the two-toed sloth is like other Xenarthra and some domestic animals. Macroscopically, the nose is like Choloepus hoffmanni, the nasal cavity to other species of Xenarthra already described in addition to domestic animals. The trachea differs from other species in terms of the number of tracheal rings and extension. As for the lungs, they were the ones that differed most when compared to other Xenarthra and to domestic animals as it was the species with the least lobation.

Species- and tissue-specific profiles and potential risks of polychlorinated biphenyls (PCBs) and their metabolites in dogs and cats.

In recent years, there has been growing concern about the long-term health effects of chemical exposure in pets, particularly dogs and cats, from sources such as pet food and house dust. Domestic cats (Felis silvestris catus) and dogs (Canis lupus familiaris) are continuously exposed to polychlorinated biphenyls (PCBs), with particular attention being paid to the toxicity of their metabolites, hydroxylated PCBs (OH-PCBs) and methylsulfonyl PCBs (MeSO2-PCBs). However, the tissue distribution and species-specific differences of these PCB metabolites in domestic animals have not been fully elucidated. This study investigates the tissue-specific profiles of PCBs, OH-PCBs, and MeSO2-PCBs by analyzing blood, brain, liver, and bile samples from dogs and cats. The analysis revealed that hexa- to octa-chlorinated OH-PCBs were the predominant congeners in the brain, liver and bile of dogs. In contrast, tri- to penta-chlorinated OH-PCBs were more prevalent in cats, with lower-chlorinated OH-PCBs tending to accumulate due to limited UDP-glucuronosyltransferase activity. In cats, OH-PCBs are more readily excreted in the bile than in dogs, probably because there are fewer higher-chlorinated thyroxine-like OH-PCBs, which are known to bind to and persist in proteins in the liver and blood. MeSO2-PCBs were detected at lower concentrations than parent PCBs and OH-PCBs and primarily accumulated in the liver due to their lipophilic nature. The consistent concentrations of MeSO2-PCBs across species, despite variations in parent PCB and OH-PCB levels, underscore species-specific differences in metabolic capacity and excretion pathways. In addition, some OH-PCB concentrations in both dog and cat brains exceeded levels known to affect neurons, suggesting the potential for neurotoxicity in these species. Therefore, continued biomonitoring and further investigation of the toxic effects of these compounds in pets is imperative.

Eficiência do óleo de candeia para melhor a resistência da madeira a fungos apodrecedores

ABSTRACT World demand for wood products and the shortage of good quality lumber species are exerting pressure on native forests. This study aimed to evaluate the efficiency of Eremanthus erythropappus crude oil in improving the resistance of Pinus taeda wood to Rhodonia (≈Postia) placenta, Gloeophyllum trabeum, Neolentinus lepideus (brown rot) and Pycnoporus sanguineus (white rot) in accelerated laboratory decay tests. Test samples measured 1.90 × 1.90 × 1.90 cm. Oil was applied to wood surfaces using a brush to obtain nominal retentions of 15, 30, 45, and 60 kg of oil per m3 of wood. After treatment, the samples reached average retentions of 16.25, 28.75, 41.50, and 53.75 kg m-3, respectively, and were submitted to a soil block decay test (accelerated decay test) for 12 weeks. Increased oil retention caused a decline in the mass loss of Pinus taeda wood exposed to the fungi tested. A retention of 16.25 kg m-3 inhibited Rhodonia placenta degradation and prevented other xylophagous fungi attacks. The most severe decay occurred with Rhodonia placenta and the least with Pycnoporus sanguineus. Oil improved Pinus taeda resistance to the decay fungi, indicating that it is useful for treating wood in contact with domestic animals and humans, since it is not toxic to them.

Cuticular composition: An alternative taxonomic approach to differentiate between Argas arboreus and Argas persicus ticks (Acari: Argasidae)

Argas arboreus and A. persicus are blood sucking ectoparasites on domestic birds in Egypt. They cause anemia in birds, in addition to transmitting a variety of pathogens that leads to economical loss in the poultry industry. It is difficult for non-taxonomists to differentiate between these species because of minor morphological characters. Therefore, it is very important to identify tick species for developing a suitable strategy to reduce risks to poultry wealth. This study characterized the female cuticular hydrocarbons of two Argas species using gas chromatography-mass spectrometry. Sixty different hydrocarbons were exclusively identified in A. arboureus, whereas only 51 in A. persicus. Some of the hydrocarbon compounds were stage-specific ones that differentiate between two species. Others shared between all feeding stages of both species that improved they are closely related ones. Genetic variability recorded its maximum value between unfed stages of the two species, and similarity reached only 25%. The present study provides the first chemotaxonomic data to differentiate between two closely related Argas species according to their cuticular hydrocarbons. Therefore, hydrocarbon composition seems to be a promising tool available as a taxonomic character, in addition improved that feeding stage was the susceptible one to be controlled.

Modulating vertebrate physiology by genomic fine-tuning of GPCR functions.

G protein-coupled receptors (GPCRs) play a crucial role as membrane receptors, facilitating the communication of eukaryotic species with their environment and regulating cellular and organ interactions. Consequently, GPCRs hold immense potential in contributing to adaptation to ecological niches and responding to environmental shifts. Comparative analyses of vertebrate genomes reveal patterns of GPCR gene loss, expansion, and signatures of selection. Integrating these genomic data with insights from functional analyses of gene variants enables the interpretation of genotype-phenotype correlations. This review underscores the involvement of GPCRs in adaptive processes, presenting numerous examples of how alterations in GPCR functionality influence vertebrate physiology or, conversely, how environmental changes impact GPCR functions. The findings demonstrate that modifications in GPCR function contribute to adapting to aquatic, arid, and nocturnal habitats, influencing camouflage strategies, and specializing in particular dietary preferences. Furthermore, the adaptability of GPCR functions provides an effective mechanism in facilitating past, recent, or ongoing adaptations in animal domestication and human evolution and should be considered in therapeutic strategies and drug development.