Domain Organization Research Articles

Making cities mental health friendly for adolescents and young adults

Urban life shapes the mental health of city dwellers, and although cities provide access to health, education and economic gain, urban environments are often detrimental to mental health1,2. Increasing urbanization over the next three decades will be accompanied by a growing population of children and adolescents living in cities3. Shaping the aspects of urban life that influence youth mental health could have an enormous impact on adolescent well-being and adult trajectories4. We invited a multidisciplinary, global group of researchers, practitioners, advocates and young people to complete sequential surveys to identify and prioritize the characteristics of a mental health-friendly city for young people. Here we show a set of ranked characteristic statements, grouped by personal, interpersonal, community, organizational, policy and environmental domains of intervention. Life skills for personal development, valuing and accepting young people’s ideas and choices, providing safe public space for social connection, employment and job security, centring youth input in urban planning and design, and addressing adverse social determinants were priorities by domain. We report the adversities that COVID-19 generated and link relevant actions to these data. Our findings highlight the need for intersectoral, multilevel intervention and for inclusive, equitable, participatory design of cities that support youth mental health.

What matters in low-threshold collaboration? Perceptions of interprofessional collaboration between education and social and healthcare professionals in Finnish primary schools

ABSTRACT This study examined the factors linked to low-threshold interprofessional collaboration in the context of Finnish primary schools. The main purpose of the study was to analyze how education and health and social care professionals perceived their mutual collaboration. The PINCOM-Q scale was used to identify factors related to interprofessional collaboration in professionals’ work settings. The results indicate that individual factors such as work motivation and personal power are prominent in low-threshold collaboration. At the group level, communication has an important role to play in interprofessional collaboration. Professionals (n = 204) perceived mutual exchange of information as an important aspect of working together. The aspects that matter in the low-threshold mode of interprofessional collaboration are a complex combination of individual, group and less obvious organizational factors, all of which both reflect and are reflected in an individual’s motivation and commitment to cooperation. The establishment of long-term and systematic low-threshold, interprofessional collaboration presupposes that individual interests are realized in good interaction in equal encounters between different organizational domains.

Rare NRPS Gene Cluster for Desferriferrichrome Biosynthesis Controls the Conflict between Trap Formation and Nematicidal Activity in Arthrobotrys oligospora.

The formation of the trapping device induced by nematodes has been assumed as an indicator for a switch from saprophytic to predacious lifestyles for nematode-trapping fungi. However, fungal nematocidal activity is not completely synonymous with fungal trap formation. We found that the predominant nematode-trapping fungus Arthrobotrys oligospora harbored a rare NRPS (Ao415) gene cluster that was mainly distributed in nematode-trapping fungi. The gene Ao415 putatively encodes a protein with a unique domain organization, distinct from other NRPSs in other fungi. Mutation of the two key biosynthetic genes Ao415 and Ao414 combined with nontarget metabolic analysis revealed that the Ao415 gene cluster was responsible for the biosynthesis of a hydroxamate siderophore, desferriferrichrome (1). Lack of desferriferrichrome (1) and its hydroxamate precursor (3) could lead to significantly increased Fe3+ content, which induced fungal trap formation without a nematode inducer. Furthermore, the addition of Fe3+ strongly improved fungal trap formation but deleteriously caused broken traps. The addition of 1 significantly attenuated trap formation but enhanced fungal nematicidal activity. Our findings indicate that iron is a key factor for trap formation and provide a new insight into the underlying mechanism of siderophores in nematode-trapping fungi.

Molecular Insights into O-Linked Sialoglycans Recognition by the Siglec-Like SLBR-N (SLBRUB10712) of Streptococcus gordonii.

Streptococcus gordonii is a Gram-positive bacterial species that typically colonizes the human oral cavity, but can also cause local or systemic diseases. Serine-rich repeat (SRR) glycoproteins exposed on the S. gordonii bacterial surface bind to sialylated glycans on human salivary, plasma, and platelet glycoproteins, which may contribute to oral colonization as well as endocardial infections. Despite a conserved overall domain organization of SRR adhesins, the Siglec-like binding regions (SLBRs) are highly variable, affecting the recognition of a wide range of sialoglycans. SLBR-N from the SRR glycoprotein of S. gordonii UB10712 possesses the remarkable ability to recognize complex core 2 O-glycans. We here employed a multidisciplinary approach, including flow cytometry, native mass spectrometry, isothermal titration calorimetry, NMR spectroscopy from both protein and ligand perspectives, and computational methods, to investigate the ligand specificity and binding preferences of SLBR-N when interacting with mono- and disialylated core 2 O-glycans. We determined the means by which SLBR-N preferentially binds branched α2,3-disialylated core 2 O-glycans: a selected conformation of the 3'SLn branch is accommodated into the main binding site, driving the sTa branch to further interact with the protein. At the same time, SLBR-N assumes an open conformation of the CD loop of the glycan-binding pocket, allowing one to accommodate the entire complex core 2 O-glycan. These findings establish the basis for the generation of novel tools for the detection of specific complex O-glycan structures and pave the way for the design and development of potential therapeutics against streptococcal infections.

Sustainable Digital Evolution: Transforming the Ukrainian Customs Service

The evolving landscape of information technologies has spurred a profound digital revolution across cultural, societal, organizational, and governmental domains. This article examines the transformative impact of Information and Communications Technology (ICT) and electronic systems within Ukrainian customs operations and their broader societal implications. At the core of this exploration lies the pivotal role of ICT integration in modernizing the Ukrainian Customs Service amidst global challenges, exemplified by the COVID-19 pandemic. The study assesses the benefits, challenges, and implications of “electronic customs” and IT-driven reforms, focusing on the three pillars of sustainability, customs operations, and digital culture. Objectives encompass an evaluation of ICT’s significance in enhancing border management efficiency, addressing challenges, and leveraging opportunities through technology-driven customs initiatives. Synthesizing academic publications and governmental reports, the article provides comprehensive insights into the transformative potential of technology in customs management. Notably, the implementation of anti-corruption measures, particularly through the Single Window system, emerges as a successful endeavor in curbing malpractices while streamlining customs procedures. However, challenges persist, including swift adoption, cyber fraud risks, and systemic issues within customs authorities, necessitating phased implementation and continuous improvement. Ethical reflections on technology integration into governance mechanisms, educational reforms, and inclusivity discussions underscore the broader societal and philosophical shifts catalyzed by electronic customs. This sustainable digital evolution emphasizes ongoing ethical considerations to navigate the evolving role of technology in shaping governance, societal integration, sustainable customs operations, and the inherent alignment of digital technologies with the key objectives of sustainable development.
 Keywords: customs operations, digital culture, sustainable development, Single Window, cybersecurity, Information and Communication Technology, societal impact, ethical framework, cultural shifts

Ethical Considerations in Social Media Utilization by Religious Organizations: An In-depth Investigation

In the contemporary landscape, social media platforms have evolved into highly effective instruments facilitating participatory communication across diverse aspects of human life. The advent of the digital revolution has introduced a novel dimension to the application of social media, extending its reach into various realms of communication, management, and development initiatives. Particularly noteworthy is the role that social media platforms play in the domain of religious communication, mobilization, and organization. Among the myriad social media platforms, Twitter and Facebook stand out as extensively utilized tools by religious organizations and leaders. Leveraging these platforms, religious entities seek to establish direct communication channels with their target groups, fostering active engagement and participation in the organizational and managerial aspects of religious institutions. This paradigm shift in communication strategies has become increasingly relevant and influential. To explore the dynamics of this intersection between social media and religious engagement, the present investigation was undertaken in Delhi, focusing on the National Capital Region (NCR) – a geographical area encompassing all major religions in the country. The study delves into the multifaceted ways in which social media platforms are employed for religious outreach, examining their impact on communication strategies, organizational dynamics, and the active involvement of the faithful within the diverse religious landscape of the NCR. Through a comprehensive analysis of the use of social media in this specific context, the research aims to contribute valuable insights into the evolving nature of religious communication in the digital age. Keywords: social media platforms, religious communication, digital revolution, national capital region, participatory engagement

Development of a Taxonomy of Policy Interventions for Integrating Nurse Practitioners into Health Systems

Background: Nurse practitioners (NPs)—nurses with advanced training who can provide and prescribe care—are increasingly prevalent internationally. The growth of NPs can be attributed to physician shortages, growing demand for health services, and the professionalization of nursing. Ensuring efficacious integration of NPs into the health system is critical for maximizing their impact on patient and system outcomes. Nonetheless, there is a dearth of information on practical policy interventions facilitating the successful integration of NPs. Effective policy interventions should capture the perspectives of actors at all levels of the health system—ie, national and organizational levels. This study aimed to delineate a taxonomy of policy interventions for integrating NPs into health systems. This paper presents a taxonomy developed among gerontology NPs in Israel. Methods: This qualitative descriptive study used multiple perspective, one-to-one interviews with four professional groups—national policy-makers, organizational administrators, NPs, and physicians. Data were analyzed using deductive content analysis. Analysis accounted for diverging and converging patterns between professional groups. Results: There were 58 participants across the four professional groups. The national-level domain interventions include marketing, workforce development, professional licensure and regulation, financial incentives, stakeholder cooperation, education and training programs, and national-level research. Organizational domain interventions included organizational guidelines, infrastructure development and resource allocation, interprofessional leadership engagement, and organizational messaging. Unit- and care-team domain interventions included interprofessional experience and exposure, team communication, and mentorship. Conclusion: The taxonomy’s trichotomy of three health system level domains describes the relationship between national and organizational policy interventions. Adopting these interventions may result in an improved response to provider shortages. Policies insufficiently addressing role clarity and workforce retention resulted in poor integration and a failure to efficaciously combat workforce shortages. Future work will expand the focus of this preliminary taxonomy by further development and testing with international participants.

Screening and identification of photoresponse factors in kiwifruit (Actinidia arguta) development.

Light is essential for kiwifruit development, in which photoresponse factors contributes greatly to the quality formation. 'Light sensitive hypocotyls, also known as light-dependent short hypocotyls' (LSH) gene family can participate in fruit development as photoresponse factor. However, the key LSH gene that determine kiwifruit development remains unclear. This study aim to screen and identify the key gene AaLSH9 in A. arguta. Genome-wide identification of the LSH gene family was used to analyse LSH genes in kiwifruit. Homologous cloning was used to confirm the sequence of candidate LSH genes. qRT-PCR and cluster analysis of expression pattern were used to screen the key AaLSH9 gene. Subcellular localization of AaLSH9 in tobacco leaves and overexpression of AaLSH9 in Arabidopsis thaliana hy5 mutant plants were used to define the acting place in cell and identify molecular function, respectively. We identified 15 LSH genes, which were divided into two sub-families namely A and B. Domain analysis of A and B showed that they contained different domain organizations, which possibly played key roles in the evolution process. Three LSH genes, AaLSH2, AaLSH9, and AaLSH11, were successfully isolated from Actinidia arguta. The expression pattern and cluster analysis of these three AaLSH genes suggested AaLSH9 might be a key photoresponse gene participating in fruit development in A. arguta. Subcellular localization showed AaLSH9 protein was located in the nucleus. The overexpression of AaLSH9 gene in Arabidopsis thaliana hy5 mutant plants partially complemented the long hypocotyls of hy5 mutant, implying AaLSH9 played a key role as photoresponse factor in cells. In addition, the seed coat color of A. thaliana over-expressing AaLSH9 became lighter than the wide type A.thaliana. Finally, AaCOP1 was confirmed as photoresponse factor to participate in developmental process by stable transgenic A. thaliana. AaLSH9 can be involved in kiwifruit (A. arguta) development as key photoresponse factor. Our results not only identified the photoresponse factors AaLSH9 and AaCOP1 but also provided insights into their key role in fruit quality improvement in the process of light response.

The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein.

Argonaute (Ago) proteins are present in all three domains of life (bacteria, archaea and eukaryotes). They use small (15-30 nucleotides) oligonucleotide guides to bind complementary nucleic acid targets and are responsible for gene expression regulation, mobile genome element silencing, and defence against viruses or plasmids. According to their domain organization, Agos are divided into long and short Agos. Long Agos found in prokaryotes (long-A and long-B pAgos) and eukaryotes (eAgos) comprise four major functional domains (N, PAZ, MID and PIWI) and two structural linker domains L1 and L2. The majority (∼60%) of pAgos are short pAgos, containing only the MID and inactive PIWI domains. Here we focus on the prokaryotic Argonaute AfAgo from Archaeoglobus fulgidus DSM4304. Although phylogenetically classified as a long-B pAgo, AfAgo contains only MID and catalytically inactive PIWI domains, akin to short pAgos. We show that AfAgo forms a heterodimeric complex with a protein encoded upstream in the same operon, which is a structural equivalent of the N-L1-L2 domains of long pAgos. This complex, structurally equivalent to a long PAZ-less pAgo, outperforms standalone AfAgo in guide RNA-mediated target DNA binding. Our findings provide a missing piece to one of the first and the most studied pAgos.

Performance of Fluxgate Magnetometer with Cu-Doped CoFeSiB Amorphous Microwire Core.

In this study, we investigated the effects of Cu doping on the performance of CoFeSiB amorphous microwires as the core of a fluxgate magnetometer. The noise performance of fluxgate sensors primarily depends on the crystal structure of constituent materials. CoFeSiB amorphous microwires with varying Cu doping ratios were prepared using melt-extraction technology. The microstructure of microwire configurations was observed using transmission electron microscopy, and the growth of nanocrystalline was examined. Additionally, the magnetic performance of the microwire and the noise of the magnetic fluxgate sensors were tested to establish the relationship between Cu-doped CoFeSiB amorphous wires and sensor noise performance. The results indicated that Cu doping triggers a positive mixing enthalpy and the reduced difference in the atomic radius that enhances the degree of nanocrystalline formation within the system; differential scanning calorimetry analysis indicates that this is due to Cu doping reducing the glass formation capacity of the system. In addition, Cu doping affects the soft magnetic properties of amorphous microwires, with 1% low-doping samples exhibiting better soft magnetic properties. This phenomenon is likely the result of the interaction between nanocrystalline organization and magnetic domains. Furthermore, a Cu doping ratio of 1% yields the best noise performance, aligning with the trend observed in the material's magnetic properties. Therefore, to reduce the noise of the CoFeSiB amorphous wire sensor, the primary goal should be to reduce microscopic defects in amorphous alloys and enhance soft magnetic properties. Cu doping is a superior preparation method which facilitates control over preparation conditions, ensuring the formation of stable amorphous wires with consistent performance.

Mental Health Among Elite Youth Athletes: A Narrative Overview to Advance Research and Practice.

Participation in sports during youth is typically beneficial for mental health. However, it is unclear whether elite sport contexts contribute to greater risk of psychological distress or disorder. The aims of this paper are to highlight conceptual issues that require resolution in future research and practice, and to examine the key factors that may contribute to the mental health of elite youth athletes (EYAs). A narrative overview of the literature combined with the clinical and research expertise of the authors. Narrative overview. Level 5. EYAs experience a range of biopsychosocial developmental changes that interact with mental health in a multitude of ways. In addition, there are various sport-specific factors that contribute to the mental health of EYAs that may become more prominent in elite contexts. These include - but are not limited to - patterns relating to athlete coping and self-relating styles, the nature of peer, parental, and coach relationships, organizational culture and performance pressures, and mental health service provision and accessibility. A range of critical factors across individual, interpersonal, organizational, and societal domains have been shown to contribute to mental health among EYAs. However, this evidence is limited by heterogeneous samples and varied or imprecise terminology regarding what constitutes "youth" and "elite" in sport. Nevertheless, it is clear that EYAs face a range of risks that warrant careful consideration to progress to best practice principles and recommendations for mental health promotion and intervention in elite youth sport. Level C.

Taming Complexity in Cybersecurity of Multihospital Systems: The Role of Enterprise-wide Data Analytics Platforms

A widely accepted notion in cybersecurity practice and research posits that complexity is detrimental to cybersecurity. While this notion mainly focuses on IT, the prevalence of complexity extends beyond IT into organizational domains. Whether the assertion that complexity is detrimental to cybersecurity holds true when applied to organizational domains remains largely unexplored. Moreover, 'complexity' and 'complicatedness' are often used interchangeably to refer to a large number of interconnected components. We build on complexity science to distinguish the two terms: complexity arises from ad-hoc, non-linear interactions among interconnected components whereas complicatedness arises from structured, linear interactions. The field lacks sufficient understanding of these distinctions and their implications for cybersecurity risks and mitigations. We theorize how an organization's complicatedness and complexity in both IT and organizational domains influence cybersecurity breaches. Our context of empirical inquiry is multihospital systems (MHSs), a complex organizational form that uses IT in complex ways. Drawing on complexity science, we posit that complicatedness in medical services, health IT, and governance arrangements of an MHS increases cybersecurity breaches. We also posit that using enterprise-wide data analytics platforms (EWDAP) to structure and control ad-hoc information sharing practices of the complicated units can reduce breaches despite adding more technology components and dependencies to enterprise IT. We test these ideas in a sample of 445 MHSs in the U.S. spanning from 2009 to 2017. Complicatedness in medical services, health IT, and governance stands out as the primary contributor to cybersecurity breaches in MHSs. Ad-hoc, non-linear interactions contribute to complexity and exacerbate the cybersecurity breach effects of complicatedness. In contrast, structured interactions, exemplified by patient data sharing through EWDAP, achieve simplification and mitigate the cybersecurity breaches associated with complicatedness.

In vivo activities of heparan sulfate differentially modified by NDSTs during development.

Heparan sulfate proteoglycans (HSPGs) serve as co-receptors for growth factor signaling during development. It is well known that the level and patterns of sulfate groups of heparan sulfate (HS) chains, or HS fine structures, have a major impact on HSPG function. On the other hand, the physiological significance of other structural features of HS, including NS/NA domain organization, remains to be elucidated. A blueprint of the HS domain structures is mainly controlled by HS N-deacetylase/N-sulfotransferases (NDSTs). To analyze in vivo activities of differentially modified HS, we established two knock-in (KI) Drosophila strains with the insertion of mouse Ndst1 (mNdst1) or Ndst2 (mNdst2) in the locus of sulfateless (sfl), the only Drosophila NDST. In these KI lines, mNDSTs are expressed from the sfl locus, in the level and patterns identical to the endogenous sfl gene. Thus, phenotypes of Ndst1 KI and Ndst2KI animals reflect the ability of HS structures made by these enzymes to rescue sfl mutation. Remarkably, we found that mNdst1 completely rescued the loss of sfl. mNdst2 showed a limited rescue ability, despite a higher level of HS sulfation compared to HS in mNdst1 KI. Our study suggests that independent of sulfation levels, additional HS structural features controlled by NDSTs play key roles during tissue patterning.

Overview on tyrosinases: Genetics, molecular biology, phylogenetic relationship.

Tyrosinases (TYRs) are enzymes found in various organisms that are crucial for melanin biosynthesis, coloration, and UV protection. They play vital roles in insect cuticle sclerotization, mollusk shell formation, fungal and bacterial pigmentation, biofilm formation, and virulence. Structurally, TYRs feature copper-binding sites that are essential for catalytic activity, facilitating substrate oxidation via interactions with conserved histidine residues. TYRs exhibit diversity across animals, plants, fungi, mollusks, and bacteria, reflecting their roles and function. Eukaryotic TYRs undergo post-translational modifications, such as glycosylation, which affect protein folding and activity. Bacterial TYRs are categorized into five types based on their structural variation, domain organization and enzymatic properties, showing versatility across bacterial species. Moreover, bacterial TYRs, akin to fungal TYRs, have been implicated in the synthesis of secondary metabolites with antimicrobial properties. TYRs share significant sequence homology with hemocyanins, oxygen-carrier proteins in mollusks and arthropods, highlighting their evolutionary relationships. The evolution of TYRs underscores the dynamic nature of these enzymes and reflects adaptive strategies across diverse taxa.

Blockchain Based Auditable Access Control For Business Processes With Event Driven Policies.

The use of blockchain technology has been proposed to provide auditable access control for individual resources. Unlike the case where all resources are owned by a single organization, this work focuses on distributed applications such as business processes and distributed workflows. These applications are often composed of multiple resources/services that are subject to the security and access control policies of different organizational domains. Here, blockchains provide an attractive decentralized solution to provide auditability. However, the underlying access control policies may have event-driven constraints and can be overlapping in terms of the component conditions/rules as well as events. Existing work cannot handle event-driven constraints and does not sufficiently account for overlaps leading to significant overhead in terms of cost and computation time for evaluating authorizations over the blockchain. In this work, we propose an automata-theoretic approach for generating a cost-efficient composite access control policy. We reduce this composite policy generation problem to the standard weighted set cover problem. We show that the composite policy correctly captures all the local access control policies and reduces the policy evaluation cost over the blockchain. We have implemented the initial prototype of our approach using Ethereum as the underlying blockchain and empirically validated the effectiveness and efficiency of our approach. Ablation studies were conducted to determine the impact of changes in individual service policies on the overall cost.

Health professionals' practices and perspectives of post-stroke coordinated discharge planning: a national survey.

Background It is best practice for stroke services to coordinate discharge care plans with primary/community care providers to ensure continuity of care. This study aimed to describe health professionals' practices in stroke discharge planning within Australia and the factors influencing whether discharge planning is coordinated between hospital and primary/community care providers. Methods A mixed-methods survey informed by the Theoretical Domains Framework was distributed nationally to stroke health professionals regarding post-stroke discharge planning practices and factors influencing coordinated discharge planning (CDP). Data were analysed using descriptive statistics and content analysis. Results Data from 42 participants working in hospital-based services were analysed. Participants reported that post-stroke CDP did not consistently occur across care providers. Three themes relating to perceived CDP needs were identified: (1) a need to improve coordination between care providers, (2) service-specific management of the discharge process, and (3) addressing the needs of the stroke survivor and family . The main perceived barriers were the socio-political context and health professionals' beliefs about capabilities . The main perceived facilitators were health professionals' social/professional role and identity, knowledge, and intentions . The organisation domain was perceived as both a barrier and facilitator to CDP. Conclusion Australian health professionals working in hospital-based services believe that CDP promotes optimal outcomes for stroke survivors, but experience implementation challenges. Efforts made by organisations to ensure workplace culture and resources support the CDP process through policies and procedures may improve practice. Tailored implementation strategies need to be designed and tested to address identified barriers.

Biomolecular Condensates: Structure, Functions, Methods of Research.

The term "biomolecular condensates" is used to describe membraneless compartments in eukaryotic cells, accumulating proteins and nucleic acids. Biomolecular condensates are formed as a result of liquid-liquid phase separation (LLPS). Often, they demonstrate properties of liquid-like droplets or gel-like aggregates; however, some of them may appear to have a more complex structure and high-order organization. Membraneless microcompartments are involved in diverse processes both in cytoplasm and in nucleus, among them ribosome biogenesis, regulation of gene expression, cell signaling, and stress response. Condensates properties and structure could be highly dynamic and are affected by various internal and external factors, e.g., concentration and interactions of components, solution temperature, pH, osmolarity, etc. In this review, we discuss variety of biomolecular condensates and their functions in live cells, describe their structure variants, highlight domain and primary sequence organization of the constituent proteins and nucleic acids. Finally, we describe current advances in methods that characterize structure, properties, morphology, and dynamics of biomolecular condensates in vitro and in vivo.

Morphology of poly-3-hexyl-thiophene blends with styrene-isoprene-styrene block-copolymer elastomers from X-ray and neutron scattering.

The nano- and micron scale morphology of poly(3-hexylthiophene) (P3HT) and polystyrene-block-polyisoprene-block-polystyrene (PS-PI-PS) elastomeric blends is investigated through the use of ultra-small and small angle X-ray and neutron scattering (USAXS, SAXS, SANS). It is demonstrated that loading P3HT into elastomer matrices is possible with little distortion of the elastomeric structure up to a loading of ∼5 wt%. Increased loadings of conjugated polymer is found to significantly distort the matrix structure. Changes in processing conditions are also found to affect the blend morphology with especially strong dependence on processing temperature. Processing temperatures above the glass transition temperature (Tg) of polystyrene and the melting temperature (Tm) of the conjugated polymer additive (P3HT) creates significantly more organized mesophase domains. P3HT blends with PS-PI-PS can also be flow-aligned through processing, which results in an anisotropic structure that could be useful for the generation of anisotropic properties (e.g. conductivity). Moreover, the extent of flow alignment is significantly affected by the P3HT loading in the PS-PI-PS matrix. The work adds insight to the morphological understanding of a complex P3HT and PS-PI-PS polymer blend as conjugated polymer is added to the system. We also provide studies isolating the effect of processing changes aiding in the understanding of the structural changes in this elastomeric conjugated polymer blend.

Molecular mechanisms of perilipin protein function in lipid droplet metabolism.

Perilipins are abundant lipid droplet (LD) proteins present in all metazoans and also in Amoebozoa and fungi. Humans express five perilipins, which share a similar domain organization: an amino-terminal PAT domain and an 11-mer repeat region, which can fold into amphipathic helices that interact with LDs, followed by a structured carboxy-terminal domain. Variations of this organization that arose during vertebrate evolution allow for functional specialization between perilipins in relation to the metabolic needs of different tissues. We discuss how different features of perilipins influence their interaction with LDs and their cellular targeting. PLIN1 and PLIN5 play a direct role in lipolysis by regulating the recruitment of lipases to LDs and LD interaction with mitochondria. Other perilipins, particularly PLIN2, appear to protect LDs from lipolysis, but the molecular mechanism is not clear. PLIN4 stands out with its long repetitive region, whereas PLIN3 is most widely expressed and is used as a nascent LD marker. Finally, we discuss the genetic variability in perilipins in connection with metabolic disease, prominent for PLIN1 and PLIN4, underlying the importance of understanding the molecular function of perilipins.

Is Education Level a Modulation Factor of Cognitive Abilities in Older Individuals?

Purpose: Similar to bilingualism, musical abilities, socio-economic status, and physical activities; education level also plays a contributing factor to cognitive reserve in an individual. Literature suggests that educational level and years of schooling enhance cognitive development and also show a correlation with better Intelligence Quotient. It is important to consider the level of education and its relationship with cognitive abilities particularly assessed on standardized cognitive tests which were not considered in earlier research.Methods: The present study recruited 25 native Kannada speakers aged 50 to 70 years and divided them into two groups based on level of education (lower education and higher education group). All the participants were assessed on Cognitive Linguistic Assessment Protocol (CLAP)-Kannada (Kamath & Prema, 2001). The performance scores were tabulated and analyzed across all the domains of the test.Results: Descriptive statistics highlighted that the higher education group had greater overall scores and also across the domains compared to their counterparts. Inferential statistics utilizing the Mann-Whitney U test found a significant difference between the two groups on overall performance and also across the domains with high effect size. Both groups performed poorly in the organization domain, attributed to task demands that need a greater level of processing skills.Conclusions: The current study highlights that higher education levels influence better cognitive function and that this effect persists with age as compared to the lower education levels group indicating higher educational levels can help an individual to resist the effects of the aging process on cognitive function decline.