Stability Domain Research Articles

Mechanical Unfolding of Network Nodes Drives the Stress Response of Protein-Based Materials.

Biomaterials synthesized from cross-linked folded proteins have untapped potential for biocompatible, resilient, and responsive implementations, but face challenges due to costly molecular refinement and limited understanding of their mechanical response. Under a stress vector, these materials combine the gel-like response of cross-linked networks with the mechanical unfolding and extension of proteins from well-defined 3D structures to unstructured polypeptides. Yet the nanoscale dynamics governing their viscoelastic response remains poorly understood. This lack of understanding is further exacerbated by the fact that the mechanical stability of protein domains depends not only on their structure, but also on the direction of the force vector. To this end, here we propose a coarse-grained network model based on the physical characteristics of polyproteins and combine it with the mechanical unfolding response of protein domains, obtained from single molecule measurements and steered molecular dynamics simulations, to explain the macroscopic response of protein-based materials to a stress vector. We find that domains are about 10-fold more stable when force is applied along their end-to-end coordinate than along the other tethering geometries that are possible inside the biomaterial. As such, the macroscopic response of protein-based materials is mainly driven by the unfolding of the node-domains and rearrangement of these nodes inside the material. The predictions from our models are then confirmed experimentally using force-clamp rheometry. This model is a critical step toward developing protein-based materials with predictable response and that can enable applications for shape memory and energy storage and dissipation.

Social Disadvantage and Disparities in Chronic Liver Disease: A Systematic Review

Introduction: Social determinants of health (SDOH) may impact chronic liver disease (CLD) outcomes but are not clearly understood. We conducted a systematic review to describe the associations of SDOH with mortality, hospitalizations, and readmissions among patients with CLD. Methods: This review was registered (PROSPERO ID: CRD42022346654) and identified articles through MEDLINE, Embase, Cochrane Library, and Scopus databases. The review included studies that reported SDOH characteristics within the domains of economic stability, health care access, education, social and community context, and the neighborhood built environment. Associated outcomes of interest were mortality, hospitalizations, or readmissions. The Cochrane Risk of Bias in Non-randomized Studies for Exposure (ROBINS-E) was used to assess study quality and risk of bias. Results: A total of 5,205 abstracts were screened, 60 articles underwent full-text review, and 27 articles were included in the final review. Poor economic stability, healthcare access, social support, and household/environmental conditions were associated with higher mortality and hospital readmissions among patients with CLD. Increasing distance (≥25 miles away) from a liver transplantation (LT) center was associated with higher mortality despite increasing access to the LT waitlist. When assessing the overall risk of bias among included studies, most had “Some Concern” (N=13, 48.1%) or “High Risk” (N=11, 40.7%) while a minority had “Very High Risk” (N=3, 11.1%). No studies were categorized as “Low Risk.” Conclusions: Unfavorable SDOH were associated with increased mortality and hospital readmissions among patients with CLD. Rigorous empirical research is needed to identify evidence-based strategies that aim to mitigate disparities among vulnerable populations.

Redox Gradient Shapes the Chemical Composition of Peatland Microbial Communities.

The response of soil carbon to climate change and anthropogenic forcing depends on the relationship between the physicochemical variables of the environment and microbial communities. In anoxic soils that store large amounts of organic carbon, it can be hypothesized that the low amount of catabolic energy available leads microbial organisms to minimize the energy costs of biosynthesis, which may shape the composition of microbial communities. To test this hypothesis, thermodynamic modeling was used to assess the link between redox gradients in the ombrotrophic peatland of the Marcell Experimental Forest (Minnesota, USA) and the chemical and taxonomic composition of microbial communities. The average amino acid composition of community-level proteins, called hereafter model proteins, was calculated from shotgun metagenomic sequencing. The carbon oxidation state of model proteins decreases linearly from -0.14 at 10 cm depth to -0.17 at 150 cm depth. Calculating equilibrium activities of model proteins for a wide range of chemical conditions allows identification of the redox potential of maximum chemical activity. Consistent with redox measurements across peat soils, this model Eh decreases logarithmically from an average value of 300 mV at 10 cm depth, close to the stability domain of goethite relative to Fe2+, to an average value of -200 mV at 150 cm, within the stability domain of CH4 relative to CO2. The correlation identified between the taxonomic abundance and the carbon oxidation state of model proteins enables predicting the evolution of taxonomic abundance as a function of model Eh. The model taxonomic abundance is consistent with the measured gene and taxonomic abundance, which evolves from aerobic bacteria at the surface including Acidobacteria, Proteobacteria, and Verrumicrobia, to anaerobes at depth dominated by Crenarchaeota. These results indicate that the thermodynamic forcing imposed by redox gradient across peat soils shapes both the chemical and taxonomic composition of microbial communities. By providing a mechanistic understanding of the relationship between microbial community and environmental conditions, this work sheds new light on the mechanisms that govern soil microbial life and opens up prospects for predicting geochemical and microbial evolution in changing environments.

Destabilizing effect of Coulomb friction induced limit cycles on sampled-data linear systems

AbstractThis paper presents a counterintuitive effect of Coulomb friction on the dynamics of sampled-data linear systems. Due to dry friction, the motion becomes sensitive to the initial conditions, and the different possible motion trajectories are separated by limit cycles. This paper shows that the linearly stable behavior of the frictionless case degrades due to the presence of dry friction and how the stable domain of operation decreases. The results are illustrated through the example of a single-degree-of-freedom system model. The presented analysis of the nonlinear system gives insight into the intricate dynamics of sampled-data systems due to the effect of dry friction, and numerical simulations verify the results.

Role of domain wall conductivity in the stability of ferroelectric domains in ferroelectric single crystals

Role of domain wall conductivity in the stability of ferroelectric domains in ferroelectric single crystals

FSH3D: 3D Representation via Fibonacci Spherical Harmonics

AbstractSpherical harmonics are a favorable technique for 3D representation, employing a frequency‐based approach through the spherical harmonic transform (SHT). Typically, SHT is performed using equiangular sampling grids. However, these grids are non‐uniform on spherical surfaces and exhibit local anisotropy, a common limitation in existing spherical harmonic decomposition methods. This paper proposes a 3D representation method using Fibonacci Spherical Harmonics (FSH3D). We introduce a spherical Fibonacci grid (SFG), which is more uniform than equiangular grids for SHT in the frequency domain. Our method employs analytical weights for SHT on SFG, effectively assigning sampling errors to spherical harmonic degrees higher than the recovered band‐limited function. This provides a novel solution for spherical harmonic transformation on non‐equiangular grids. The key advantages of our FSH3D method include: 1) With the same number of sampling points, SFG captures more features without bias compared to equiangular grids; 2) The root mean square error of 32‐degree spherical harmonic coefficients is reduced by approximately 34.6% for SFG compared to equiangular grids; and 3) FSH3D offers more stable frequency domain representations, especially for rotating functions. FSH3D enhances the stability of frequency domain representations under rotational transformations. Its application in 3D shape reconstruction and 3D shape classification results in more accurate and robust representations. Our code is publicly available at https://github.com/Miraclelzk/Fibonacci-Spherical-Harmonics.

Transient behavior of pumped storage-wind hybrid power system under grid-connected operation condition

The large-scale grid connection of renewable energy represented by wind power threatens the safe and stable operation of electric system. The vigorous development pumped storage power station (PSPS) is a global consensus to support the grid-connected of renewable energy. This paper investigates the transient behavior of pumped storage-wind (PSW) hybrid power system under grid-connected operation condition (GCOC). Firstly, a model of PSW hybrid power system under GCOC is constructed. The stable domain is determined according to Hopf bifurcation theory. The influence of system parameters on the stability of PSW hybrid power system under GCOC is analyzed. A synergetic control strategy is designed to improve the dynamic performance of the system. Then, the mechanism of multi-time-scale dynamic response is revealed. Finally, the transient behavior of PSPS under different application scenarios and the interaction between PSPS and wind power station (WPS) are studied. The results show that the stability of PSW hybrid power system under GCOC can be judged by stable domain. The designed synergetic control strategy can improve the dynamic performance of the system. The dynamic response is composed of three oscillations with different frequencies, namely, mass oscillation at low frequency, water hammer oscillation at medium frequency, and electrical oscillation at high frequency. The principal oscillation is caused by water hammer. The interaction between PSPS and WPS has a significant effect on the transient behavior. By adjusting the interaction between PSPS and WPS, the system can absorb more wind power while ensuring safe and stable operation, which can increase profits and avoid unnecessary operation oscillations. The analytical methods and results presented in this paper are of great significance for improving the ability of power grid to accept renewable energy.

Efficient Nonlinear Model Predictive Path Tracking Control for Autonomous Vehicle: Investigating the Effects of Vehicle Dynamics Stiffness

Motion control is one of the three core modules of autonomous driving, and nonlinear model predictive control (NMPC) has recently attracted widespread attention in the field of motion control. Vehicle dynamics equations, as a widely used model, have a significant impact on the solution efficiency of NMPC due to their stiffness. This paper first theoretically analyzes the limitations on the discretized time step caused by the stiffness of the vehicle dynamics model equations when using existing common numerical methods to solve NMPC, thereby revealing the reasons for the low computational efficiency of NMPC. Then, an A-stable controller based on the finite element orthogonal collocation method is proposed, which greatly expands the stable domain range of the numerical solution process of NMPC, thus achieving the purpose of relaxing the discretized time step restrictions and improving the real-time performance of NMPC. Finally, through CarSim 8.0/Simulink 2021a co-simulation, it is verified that the vehicle dynamics model equations are with great stiffness when the vehicle speed is low, and the proposed controller can enhance the real-time performance of NMPC. As the vehicle speed increases, the stiffness of the vehicle dynamics model equation decreases. In addition to the superior capability in addressing the integration stability issues arising from the stiffness nature of the vehicle dynamics equations, the proposed NMPC controller also demonstrates higher accuracy across a broad range of vehicle speeds.

Exploring soil pedogenesis through frequency-dependent magnetic susceptibility in varied lithological environments

AbstractThe use of percent frequency-dependent magnetic susceptibility (χfd%) is well-established for detecting superparamagnetic (SP) components in fine-grained soils and sediments. This study employs χfd% as a direct indicator of pedogenetic processes in soils from the Moroccan Rif region. Three soil transects (T1, T2, and T3), each comprising four soil cores with depths reaching 100 to 120 cm, were sampled from distinct lithological formations within an area subject to moderate to intense erosion. A total of 272 soil samples were collected and analyzed using MS2 Bartington Instruments, providing values to calculate χfd% and identify ultrafine ferrimagnetic minerals (SP, < 0.03 μm). In Quaternary fluvial terraces (T1) soils, approximately 60% of the samples indicate a mixture of SP, multidomain (MD), and Single Stable Domain (SSD) magnetic grains, while 30% contained coarser MD grains. Only 10% of the samples exhibit predominantly superparamagnetic (SP) grains. Soils on marly substrates (T2) showed 90% of samples with a combination of SP, MD, and SSD, and just 10% had SP grains. In contrast, soils from Villafranchian sandy deposits displayed χfd% values exceeding 10% in over 50% of samples, indicating that almost all iron components consist of SP grains. Physico-chemical analyses of the soils in profiles T1, T2, and T3 reveal distinct characteristics, including variations in clay content, organic matter, nutrient levels, and proportions of free and total iron. These results are important for understanding soil evolution and pedogenesis, with profiles T1 and T3 showing advanced development marked by high mineral iron, clay, and organic matter content. In contrast, profile T2 reflects a weak stage, influencing nutrient availability and contributing to overall soil dynamics in the respective profiles. The results of this study suggest that magnetic susceptibilities in these samples primarily originate from pedogenetic sources, revealing significantly advanced pedogenesis compared to T1 and T2 soils. The findings of this study align with previous research on soil erosion and degradation in the region, demonstrating that soils developed on terraces and marly substrates are more degraded and less stable than those on sandy substrates. This study underscores the utility of magnetic susceptibility as a rapid and effective indicator for initial soil assessment and gauging the degree of pedogenesis.

Stalling out chromatin machinery—Oncohistone mutation disrupts heterochromatin memory

In this issue, Sinha etal.1 use cellular chromatin reporter assays along with CRISPR gene editing to reveal that the histone H3.3K36M oncohistone mutation disrupts epigenetic memory and stability of H3K9me3 domains by blocking transitions into a stably repressed state.

Small-Signal Stability Analysis and Voltage Control Parameter Design for DC Microgrids

Small-signal instability issues will occur in the DC microgrid when the high-frequency oscillation peaks of the voltage closed-loop transfer function are not effectively suppressed. To ensure the small-signal stability of DC microgrids, the concept of a small-signal stability domain for voltage control parameters is proposed. Based on the voltage closed-loop transfer function, a small-signal-stability-domain-solving algorithm is proposed. With this stability domain, the impact of voltage-proportional coefficient and voltage-integral coefficient on oscillation frequency (or damping factor) is quantitatively analyzed. In addition, the influence of current control parameters on the small-signal stability boundary is also analyzed. With the introduction of this stability domain, a design method for voltage control parameters has been proposed. The voltage control parameters, which are quantitatively designed by this method, are able to maintain the small-signal stability of the system. Finally, based on the RT-BOX hardware-in-the-loop experimental platform, a switching model for a typical DC microgrid is established. Additionally, the effectiveness of the proposed algorithm and designed control parameters is verified by multiple sets of experimental results.

A Simple Analysis of the Second (Extra) Disulfide Bridge of VHHs.

Camelids produce a special type of antibody, known as VHHs, that has lost the VL domain, providing a more optimised VH domain. This particular highly stable antibody domain has interesting properties for biotechnological development. Ordinarily, those molecules possess only one disulphide bridge, but surprisingly, at least a quarter of these VHHs have a second one. Curiously, this does not seem to be essential for the stability and the function of this domain. In an attempt to characterise precisely the role and impact of this disulphide bridge at the molecular level, several in silico mutants of a VHH were created to disrupt this second disulphide bridge and those systems were submitted to molecular dynamics simulation. The loss of the second disulphide bridge leads to an increase in the flexibility of CDR1 and CDR3 and an unexpected rigidification of CDR2. Local conformational analysis shows local differences in the observed protein conformations. However, in fact, there is no exploration of new conformations but a change in the equilibrium between the different observed conformations. This explains why the interaction of VHHs is not really affected by the presence or absence of this second bridge, but their rigidity is slightly reduced. Therefore, the additional disulphide bridge does not seem to be an essential part of VHHs function.

Research on the complex dynamical behavior of H-bridge inverter with RLC load

Complex dynamical behaviors such as bifurcation and chaos exist in H-bridge inverter with RLC load, and these nonlinear behaviors will greatly increase the harmonic content of the output current and reduce the stability and reliability of the system. In this paper, a PI controller is added to widen the stable operation domain of the system. The stroboscopic mapping theory is used to model the system, the nonlinear dynamic behavior of the inverter is investigated by the bifurcation diagram, the folding diagram and the phase trajectory diagram are used for comparative verification, and the TDFC method is introduced to inhibit the chaotic behavior of the inverter, which further improves the stable range of the system operation. The fast-change stability theorem is used to analyze the stability of the system theoretically and verify the correctness of the numerical simulation. Therefore, the conclusions of the study provide a reliable theoretical basis for the design of the inverter system, which has important theoretical significance and practical value.

Comprehensive design method of controller parameters for three‐phase LCL‐type grid‐connected inverter based on D‐partition

AbstractThe LCL‐type inverter is a core component in grid‐connected renewable energy systems, with its performance heavily influenced by the controller. Conventional design methods of controller parameters generally rely on approximation or trial and error, making it difficult to optimize parameters for multiple performance indices. This paper proposes a comprehensive design method of controller parameters for a three‐phase LCL‐type grid‐connected inverter based on the D‐partition method, obtaining a multi‐objective parameter stability domain of controller parameters that simultaneously satisfies multiple performance indices, such as gain margin, phase margin, and current loop bandwidth. The stability domain can be visually presented, which can avoid repetitive adjustments. Additionally, the variation trend of the stability domain when there are deviations in the LCL filter parameters is analyzed. Finally, the accuracy and effectiveness of the proposed design method are validated through simulations and experiments, achieving precise parameter design for the controller of LCL grid‐connected inverters even in the presence of deviations in filter parameters.

Symmetry-breaking bifurcations of pure-quartic solitons in dual-core couplers.

We investigate spontaneous symmetry- and antisymmetry-breaking bifurcations of solitons in a nonlinear dual-core waveguide with the pure-quartic dispersion and Kerr nonlinearity. Symmetric, antisymmetric, and asymmetric pure-quartic solitons (PQSs) are found, and their stability domains are identified. The bifurcations for both the symmetric and antisymmetric PQSs are of the supercritical type (alias phase transitions of the second kind). Direct simulations of the perturbed evolution of PQSs corroborate their stability boundaries predicted by the analysis of small perturbations.

Graphene-enhanced ferroelectric domain wall high-output memristor

Recent studies on memristive materials and technologies have expanded beyond conventional memory elements, driven by their potential application in novel information processing concepts. Among these materials, conductive domain walls in ferroics are especially promising, offering conductive tunability suitable for reconfigurable multi-state devices. However, challenges such as domain stability, time-dependent conductivity, and low current output have impeded progress in the field. Here, we study the graphene/Pb(Zr,Ti)O3/SrRuO3 system, which demonstrates robust domain wall conduction up to 100 nA/μm2 for 2 V bias, while addressing the critical issue of stability of switched domains. The introduction of graphene electrodes enhances low-voltage stochastic domain formation with limited domain expansion that promotes the emergence of multi-domain states. The developed micrometer sized capacitor devices enable electrically programmable multiple distinct conduction states with robust retention combined with high current output and low operation voltage. These features are highly desirable for memristors and mark the significant potential of domain wall electronics for neuromorphic computing.

Ligand-induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity.

γ-Hydroxybutyric acid (GHB) analogs are small molecules that bind competitively to a specific cavity in the oligomeric CaMKIIα hub domain. Binding affects conformation and stability of the hub domain, which may explain the neuroprotective action of some of these compounds. Here, we describe molecular details of interaction of the larger-type GHB analog 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid (PIPA). Like smaller-type analogs, PIPA binding to the CaMKIIα hub domain promoted thermal stability. PIPA additionally modulated CaMKIIα activity under sub-maximal CaM concentrations and ultimately led to reduced substrate phosphorylation. A high-resolution X-ray crystal structure of a stabilized CaMKIIα (6x mutant) hub construct revealed details of the binding mode of PIPA, which involved outward placement of tryptophan 403 (Trp403), a central residue in a flexible loop close to the upper hub cavity. Small-angle X-ray scattering (SAXS) solution structures and mass photometry of the CaMKIIα wild-type hub domain in the presence of PIPA revealed a high degree of ordered self-association (stacks of CaMKIIα hub domains). This stacking neither occurred with the smaller compound 3-hydroxycyclopent-1-enecarboxylic acid (HOCPCA), nor when Trp403 was replaced with leucine (W403L). Additionally, CaMKIIα W403L hub was stabilized to a larger extent by PIPA compared to CaMKIIα hub wild type, indicating that loop flexibility is important for holoenzyme stability. Thus, we propose that ligand-induced outward placement of Trp403 by PIPA, which promotes an unforeseen mechanism of hub domain stacking, may be involved in the observed reduction in CaMKIIα kinase activity. Altogether, this sheds new light on allosteric regulation of CaMKIIα activity via the hub domain.

Synthesis of value-added uridine 5'-diphosphate-glucose from sucrose applying an engineered sucrose synthase counteracts the activity-stability trade-off

The one-step deconstruction of sucrose into uridine 5′-diphosphate-glucose (UDP-Glc), an important sugar donor for transglycosylation, employing sucrose synthase (Susy) is emerging as a valuable sucrose utilization process. The insufficient activity and stability of Susy limit the productivity of UDP-Glc from sucrose. Here, an engineered Susy (SusyM6) that counteracted the activity-stability trade-off was developed with the half-life time and activity being 43-fold and 1.4-fold of wild-type, respectively. Tighter hydrophobic patches and stabilization of the SSN2 domain contributed to greater activity and stability. The use of SusyM6 in UDP-Glc production resulted in a satisfactory space-time yield of 73 g/L/h within 1 h. The cascade of different biocatalysts with SusyM6, focusing on utilizing two products of sucrose decomposition, fructose and UDP-Glc, expanded sucrose utilization, efficiently promoting the UDP-Glc productivity and giving a cost-effective method for UDP-galactose (UDP-Gal) synthesis. This study demonstrated promising green pathways for producing multiple value-added products from sucrose using Susy.

Drop-on-Demand 3D Printing of Programable Magnetic Composites for Soft Robotics

Soft robotics have become increasingly popular as a versatile alternative to traditional robotics. Magnetic composite materials, which respond to external magnetic fields, have attracted significant interest in this field due to their programmable two-way actuation and shape-morphing capabilities. Additive manufacturing (AM), also known as 3D printing, allows for the incorporation of different functional composite materials to create active components for soft robotic systems. However, current AM methods have limitations, especially when it comes to printing smart composite materials with high functional material content. This is a key requirement for enhancing responsiveness to external stimuli. Commonly used AM methods for smart magnetic composites, such as direct ink writing (DIW), confront challenges in achieving discontinuous printing, and enabling multi-material control at the voxel level, while some AM techniques are not suitable for producing composite materials. To address these limitations, we employed high-viscosity drop-on-demand (DoD) jetting and developed versatile programmable magnetic composites filled with micron-sized hard magnetic particles. This method bridges the gap between conventional ink-jetting and DIW, which require inks with viscosities at opposite ends of the spectrum. This high-viscosity DoD jetting enables continuous, discontinuous, and non-contact printing, making it a versatile and effective method for printing functional magnetic composites even with micron-sized fillers. Furthermore, we demonstrated stable magnetic domain programming and two-way shape-morphing actuations of printed structures for soft robotics. In summary, our work highlights high-viscosity DoD jetting as a promising method for printing functional magnetic composites and other similar materials for a wide range of applications.

Antagonistic roles of tau and MAP6 in regulating neuronal development.

Association of tau (encoded by Mapt) with microtubules causes them to be labile, whereas association of MAP6 with microtubules causes them to be stable. As axons differentiate and grow, tau and MAP6 segregate from one another on individual microtubules, resulting in the formation of stable and labile domains. The functional significance of the yin-yang relationship between tau and MAP6 remains speculative, with one idea being that such a relationship assists in balancing morphological stability with plasticity. Here, using primary rodent neuronal cultures, we show that tau depletion has opposite effects compared to MAP6 depletion on the rate of neuronal development, the efficiency of growth cone turning, and the number of neuronal processes and axonal branches. Opposite effects to those seen with tau depletion were also observed on the rate of neuronal migration, in an in vivo assay, when MAP6 was depleted. When tau and MAP6 were depleted together from neuronal cultures, the morphological phenotypes negated one another. Although tau and MAP6 are multifunctional proteins, our results suggest that the observed effects on neuronal development are likely due to their opposite roles in regulating microtubule stability.