Dog Population Research Articles

Phylogenetic analysis linked fatal neurologic disease in leopards (Panthera pardus) to Asia-5 lineage of canine distemper virus in Nepal

Canine distemper virus (CDV) is responsible for a highly contagious and often fatal neurological disease that affects various carnivores, including domestic dogs. In Nepal, recent reports of CDV exposure and illness in leopards (Panthera pardus) have raised concerns about the transmission of the virus among domestic dogs and wild carnivores. To investigate the genetic lineage and spread of CDV, our study utilized archived post-mortem samples from four leopards that exhibited clinical signs suggestive of canine distemper infection. These leopards were rescued in the Palpa, Dolakha, Kathmandu, and Parbat districts. Our phylogenetic analysis revealed that the CDV strains circulating among the leopards belong to the Asia-5 lineage, which is also prevalent among dogs and wild carnivores in Nepal and neighboring India. The genetic relatedness between the leopard CDV sequences and those from both dogs and other carnivores within the Asia-5 lineage suggests that leopards in Nepal may have acquired the virus from multiple sources, potentially facilitated by their generalist dietary habits preying on dogs and even mesocarnivores. Furthermore, we inspected specific amino acid substitution in the hemagglutinin gene of leopard CDV, which also suggests possible transmission from both domestic dogs and non-canid hosts, although further research is needed to draw definitive conclusions. Given the vulnerable state of the leopard population in Nepal, already threatened by poaching and retaliatory killing, the emergence of CDV as a potential novel threat is deeply concerning. Comprehensive surveillance studies are essential to understand the dynamics of CDV spillover and to develop informed interventions. Urgent measures, including vaccination programs and effective control of the dog population, are needed to mitigate the impact of this disease and safeguard the future of Nepal's leopards and other wild carnivores.

Thromboelastography in dogs with idiopathic epilepsy treated with phenobarbital monotherapy

AbstractBackgroundThromboelastography (TEG) is an effective technique to assess the efficiency of coagulation. Phenobarbital (PB) can induce hematological and coagulation disorders in both animals and humans, but its effects on hemostasis have been little investigated and are poorly understood in dogs.ObjectivesThe aim of this article was to assess coagulation using TEG in a population of dogs with idiopathic epilepsy treated with PB.Material and MethodsProspective observational study. TEG was performed in blood samples from dogs with idiopathic epilepsy that were divided in three groups: Two groups of treated dogs that were on phenobarbital treatment for less or more than 6 months, and a control group of healthy dogs. Duration of treatment, dose, phenobarbital serum concentration and selected hematological and biochemical parameters were evaluated and correlated with the TEG results.ResultsNo statistically significant differences were found between groups. None of the animals appeared to be in a hypo‐ or hypercoagulable state, however 9/19 (47,4%) dogs were classified as hyper‐fibrinolytic. A statistically significant negative relationship between MA and G values and increased fibrinolytic activity (LY30) were found. No statistically significant relationship was found between PB dose or PB blood levels and TEG parameters in either group. No bleeding complications were observed.DiscussionThe rise in fibrinolysis might be due to hepatic damage from PB, as indicated by elevated liver enzymes in many dogs with abnormal fibrinolytic patterns. Although TEG showed hyperfibrinolysis in some dogs, the presence of primary or secondary hyperfibrinolysis could not be confirmed due to the lack of D‐dimer measurements and liver biopsy. TEG’s sensitivity compared to other fibrinolysis biomarkers like PAP might also affect results.ConclusionsTThe cause of hyperfibrinolysis in epileptic dogs treated with phenobarbital remains unclear, with potential links to hepatic effects or handling, and further research is needed to assess its clinical significance.

PSIX-7 A multi-strain probiotic modifies fecal microbial populations of healthy adult dogs

Abstract Probiotics can modulate the intestinal microbiota and benefit the health of the host, whereas environmental stressors can negatively impact the composition of the intestinal microbiota. Lactic acid bacteria (LAB), most commonly Lactobacillus and Bifidobacterium species, are well-known organisms with probiotic potential. The objective of this study was to determine the impact on the fecal microbial population of healthy dogs, after ingestion of a probiotic blend of Lactobacillus acidophilus-286, Bifidobacterium animalis subs. Lactis-689, and Enterococcus faecium-202. Adult dogs [n = 30; 15 male, 15 female; mean body weight (BW) = 14.7 kg; mean age = 3.3 yr) were enrolled in this placebo-controlled and blinded study. After a 7-d washout period, dogs were allocated into two groups similar in terms of breed, BW, age, and sex. Dogs were fed a complete dry extruded diet and at time 0 h on d 0 received orally 4 g of a placebo paste or probiotic paste corresponding to the target dose of 4x107 total CFU. Administration of the placebo or probiotic was repeated 12 h and 24 h after the first dose. Afterward, fecal scores were evaluated daily for 7 d. Fresh fecal samples were collected on d 0, 3, and 7 for microbial enumerations and fecal metabolite measurements. Fecal microbial count data did not conform to the assumptions of normality and homogeneity of variances, consequently, non-parametric tests were performed for comparisons. Fecal scores, dry matter, and metabolites were not different between groups. Fecal counts of Lactobacillus spp., Bifidobacterium spp., and Enterococcus spp. were greater on both d 3 and d 7 (P < 0.01) in dogs that consumed probiotics. Fecal counts of both Escherichia coli and Clostridium perfringens were fewer (P < 0.01) in dogs that consumed probiotics. In conclusion, the consumption by dogs on d 0 of a probiotic blend of Lactobacillus acidophilus-286, Bifidobacterium animalis subs. Lactis-689, and Enterococcus faecium-202 modified the intestinal microbiota up to 7 d after consumption, as evidenced by the increase of lactic acid bacteria and the decrease of Escherichia coli and Clostridium perfringens in feces.

Dog Domestication Strongly Relied on Translation Regulation According to Differential Gene Expression Analysis.

Domestication of dogs from their shared ancestors with wolves occurred more than 15,000 years ago and affected many characteristics of the species. We analyzed the blood RNA sequence data of 12 dogs and 11 wolves from Europe and Asia to shed more light on the domestication history of dogs. We implemented a differential gene expression analysis, a weighted gene correlation network analysis, gene ontology and genetic pathway analyses. We found that both the sample origin (Europe or Asia) and the species had a significant effect on the blood gene expression profiles of the animals. We identified 1567 differentially expressed genes between wolves and dogs and found several significantly overrepresented gene ontology terms, such as RNA polymerase II transcription regulatory region sequence-specific DNA binding or translation. We identified 11 significant gene co-expression networks, hosting a total of 4402 genes, related to DNA replication, metabolism of RNA or metabolism of proteins, for example. Our findings suggest that gene expression regulation played a cardinal role in dog domestication. We recommend further diversifying the analyzed dog and wolf populations in the future by including individuals from different dog breeds and geographical origins, in order to enhance the specificity of detecting significant, true positive genes related to domestication as well as to reduce the false positive rate.

The Welfare of Dogs and Cats in the European Union: A Gap Analysis of the Current Legal Framework.

Companion animals, especially dogs and cats, have increasingly been recognized as moral subjects and valued as family members by European citizens. This new role encourages policy makers to face the many companion animals' welfare issues not yet covered by the EU legislation. The main gaps in the protection of dog and cat welfare during their all lifespan have been identified: unhealthy and unethical breeding practices, irresponsible sale and ownership, transport for commercial and non-commercial purposes, training methods, working dog conditions and rights, regulation of dog and cat shows and competitions, the therapeutic neglect, dog and cat end-of-life care, shelter management legislation and the free-ranging cat and dog population rights. The EU legislation framework is still very weak and far from establishing a harmonized approach, promoting a high standard of care and protection across Member States. We conclude that education and awareness regarding responsible pet ownership and the need for a One Welfare approach have a high value in finding adequate solutions, especially when poor human social welfare affects companion animal welfare. Given the link between human and companion animal welfare, the use of stakeholders' involvement strategies and a transdisciplinary approach appear crucial for the development of an EU legal framework for the well-being of dogs and cats.

Magnetic resonance imaging findings in dogs with steroid-responsive meningitis-arteritis in the UK and their clinical significance: 53 cases (2013-2021).

To describe the MRI findings in a UK referral population of dogs with steroid-responsive meningitis-arteritis and to determine if they were associated with any specific clinical features or outcomes. We performed a multi-centre retrospective case series of dogs diagnosed with steroid-responsive meningitis-arteritis in the UK that underwent MRI. Blinded consensus review of the MRI studies was performed and the findings described. The presence or absence of specific MRI abnormalities were analysed for significant associations with presenting signs, results of investigations or case outcomes. Fifty-three dogs were included. The most common MRI findings were paravertebral muscle changes (30/53; 56.6%), meningeal contrast enhancement (13/41; 31.7%) and spinal cord parenchymal T2-W hyperintensity (15/53; 28.3%). Haemorrhage was observed in five of 53 (9.4%) cases - three intradural-extramedullary, one intramedullary and one extradural. Following binary logistic regressions, T2-W spinal cord parenchymal hyperintensity had a significant positive association with paresis/paralysis (odds ratio 14.86, 95% confidence interval 1.42 to 154.99) as did haemorrhage (odds ratio 16.12, confidence interval 2.05 to 126.73). Fifty-two (98.1%) dogs survived to discharge. Relapse occurred in nine of 29 (31.0%) dogs with sufficient follow-up, and no MRI finding had a significant relationship with its occurrence. Magnetic resonance imaging findings for steroid-responsive meningitis-arteritis can be severe and extensive, as can the clinical presentation. The presence of paresis/paralysis should raise concern for haemorrhage, though most dogs still have a good prognosis.

Study of dog population dynamics and rabies awareness in Thailand using a school-based participatory research approach

Rabies is a neglected disease primarily related to dog-mediated transmission to humans. Accurate dog demographic and dynamic data are essential for effectively planning and evaluating population management strategies when designing interventions to prevent rabies. However, in Thailand, longitudinal survey data regarding dog population size are scarce. A school-based participatory research (SBPR) approach was conducted to survey owned dogs for one year in four high-risk provinces (Chiang Rai, Surin, Chonburi, and Songkhla) of Thailand, aiming to understand dog population dynamics and raise awareness about rabies. ‘Pupify’ mobile application was developed to collect data on dog population and observe the long-term population dynamics in this study. At the end of the data collection period, telephone interviews were conducted to gain insight into contextual perceptions and awareness regarding both animal and human rabies, as well as the social responsibility of dog owners in disease prevention and control. Among 303 high school students who registered in our study, 218 students reported at least one update of their dog information throughout the one-year period. Of 322 owned dogs from our survey, the updates of dog status over one year showed approximately 7.5 newborns per 100-dog-year, while deaths and missing dogs were 6.2 and 2.7 per 100-dog-year, respectively. The male to female ratio was approximately 1.8:1. Twenty-three students (10%) voluntarily participated and were interviewed in the qualitative study. The levels of rabies awareness and precautions among high-school students were relatively low. The high dropout rate of the survey was due to discontinuity in communication between the researcher and the students over the year. In conclusion, this study focused on using the SBPR approach via mobile application to collect data informing dog population dynamics and raising awareness regarding rabies in Thailand Other engaging platforms (e.g. Facebook, Instagram, Twitter, and other popular applications) is necessary to enhance communication and engagement, thereby sustaining and maintaining data collection. Further health education on rabies vaccination and animal-care practices via social media platforms would be highly beneficial. For sustainable disease control, engaging communities to raise awareness of rabies and increase dog owners’ understanding of their responsibilities should be encouraged.

Prevalence of respiratory signs in dogs with chronic gastroenteropathy in a referral population in the United Kingdom: 42 cases (2020).

To determine the prevalence of respiratory signs in a referral population of dogs with chronic gastroenteropathy in the United Kingdom, and to describe the epidemiology and clinical presentation of the affected dogs. Retrospective review of canine patients diagnosed with chronic gastroenteropathy in a referral centre in the United Kingdom. Data included signalment, gastrointestinal and respiratory signs, blood analysis results and, where present, diagnostic imaging techniques, endoscopy, videofluoroscopic swallow study, bronchoalveolar lavage results and treatment response. To be included, a follow-up of a minimum of 3 months was required. Cases were excluded if there was no minimum diagnostic dataset, the aetiology of the respiratory signs was other than gastrointestinal disease or a non-gastrointestinal systemic disease was the cause of the gastrointestinal signs. One hundred and forty-eight dogs were included and 42 (28.4%) had concurrent respiratory signs. Gagging (69%) and vomiting (52.4%) were the most common gastrointestinal signs, while coughing (50%), tachypnoea (33.3%) and reverse sneezing (30.9%) were the most common respiratory signs. Abdominal ultrasound was performed in 31 (73.8%) while CT scan was the most common modality for thoracic (62.5%) and head (21.9%) imaging. Endoscopic procedures included gastrointestinal (54.8%), bronchoscopy (19%) and rhinoscopy (23.8%). Dietary modification was the most common treatment (66.67%). Eleven dogs (26.2%) had a relapse of the respiratory signs during the follow-up period, of which, nine (81.8%) had a concurrent relapse of the gastrointestinal signs. Concurrent respiratory signs in dogs with chronic gastroenteropathy are frequent. A clinical improvement of these respiratory signs might be achieved during the therapeutic management of gastrointestinal disorders.

Species-level characterization of the core microbiome in healthy dogs using full-length 16S rRNA gene sequencing.

Despite considerable interest and research in the canine fecal microbiome, our understanding of its species-level composition remains incomplete, as the majority of studies have only provided genus-level resolution. Here, we used full-length 16S rRNA gene sequencing to characterize the fecal microbiomes of 286 presumed healthy dogs living in homes in North America who are devoid of clinical signs, physical conditions, medication use, and behavioral problems. We identified the bacterial species comprising the core microbiome and investigated whether a dog's sex & neuter status, age, body weight, diet, and geographic region predicted microbiome variation. Our analysis revealed that 23 bacterial species comprised the core microbiome, among them Collinsella intestinalis, Megamonas funiformis, Peptacetobacter hiranonis, Prevotella copri, and Turicibacter sanguinis. The 23 taxa comprised 75% of the microbiome on average. Sterilized females, dogs of intermediate body sizes, and those exclusively fed kibble tended to harbor the most core taxa. Host diet category, geographic region, and body weight predicted microbiome beta-diversity, but the effect sizes were modest. Specifically, the fecal microbiomes of dogs fed kibble were enriched in several core taxa, including C. intestinalis, P. copri, and Holdemanella biformis, compared to those fed raw or cooked food. Conversely, dogs on a raw food diet exhibited higher abundances of Bacteroides vulgatus, Caballeronia sordicola, and Enterococcus faecium, among others. In summary, our study provides novel insights into the species-level composition and drivers of the fecal microbiome in healthy dogs living in homes; however, extrapolation of our findings to different dog populations will require further study.

Molecular prevalence of Dirofilaria sp. hongkongensis, among the dog population in Thiruvananthapuram, India

Human dirofilariasis is an emerging vector-borne zoonotic parasitic disease in India. Humans are accidental hosts. Symptomatic dirofilariasis, although uncommon is typically manifested in humans as pulmonary, ocular, or subcutaneous nodules. The present research reports the prevalence of the species, Dirofilaria sp. hongkongensis among the dog population in the Kani tribal settlements within the Agasthyamala Biosphere Reserve located at the southernmost end of the Western Ghats, Kerala. To study the prevalence, we conducted a random blood survey among the dog population in these tribal settlements in Thiruvananthapuram. Whole blood was collected from stray and domesticated animals. Genomic DNA was extracted and the microfilariae in the blood were characterized using the slide smear and COI-based marker specific to nematodes. After processing the data from the Sanger sequencer using BLAST, the sequences were submitted to GenBank. Over 25 % of dogs were found positive for Dirofilaria sp. hongkongensis, which is genetically identical to the strain causing human dirofilariasis as is shown by the phylogenetic analysis. The study reveals that the Kani tribes, who reside in deep forests, are particularly vulnerable to this strain of Dirofilaria sp. hongkongensis. Human case reports of this specific strain have been recorded in Kerala over the past decade. The effective elimination is ultimately impacted by a lack of knowledge regarding the mosquito vectors. Dirofilariasis in dogs and humans is on the rise, which demands both active disease surveillance and proper treatment.

A cross-sectional epidemiological study of animal bite victims in district hospital Alwar, Rajasthan, India

Background: Rabies, an invariably fatal viral disease, transmitted to humans through animal bites, mostly dogs. According to WHO, each year, 23000-25000 people die in the SEA region due to rabies. These accounts for approximately 45% human deaths due to rabies worldwide. Objective was to study epidemiological trend of animal bite in Alwar district hospital. Methods: A retrospective, record-based study was conducted in 200 animal bite victims presenting to district hospital Alwar, Rajasthan wherein information on monthly number of animal bite cases was collected from records section of the hospital. Information on annual number of animal bite cases was available from the annual report of the hospital from 2018 onwards, this data was used for studying the monthly variation in animal bite cases. Data were compiled and analyzed using Microsoft Excel. Results: Among 200 victims 76.3% were males, 56.7% belongs to 15-45 years, 61.6% were living in urban area, 31.3% and 21.7% of the victims were agriculture worker and laborers respectively. Among 95.8% victims bitten by dog, 89% of them were stray. 89.4% had category III bite, lower extremity affected in 60.8%, fate of animal was unknown in 78.6% and 46% of the victims were bitten during evening hours. Conclusions: Majority of bites were by stray dogs, unprovoked and category III bites. This indicates the need of large amount of anti-rabies serum or HRIG thereby increasing the cost of management of animal bite cases. There is a need to control stray dog population and immunize pet dogs.

Metabarcoding study of potential pathogens and zoonotic risks associated with dog feces in Seoul, South Korea.

A significant portion of South Korea's population, approximately a quarter, owns pets, with dogs being the most popular choice among them. However, studies analyzing the fecal organism communities of dogs in South Korea are lacking, and limited efforts have been exerted to identify pathogens with potential zoonotic implications. Therefore, this study aimed to investigate potential pathogens using metabarcoding analysis and evaluate the risk of zoonotic diseases in dog feces in Seoul, South Korea. Fecal samples were collected from both pet and stray dogs in the Mapo district of Seoul. Next-generation sequencing (NGS) was utilized, employing 16S rRNA amplicon sequencing to identify prokaryotic pathogens, and 18S rRNA amplicon sequencing for eukaryotic pathogens. The data obtained from the QIIME2 pipeline were subjected to various statistical analyses to identify different putative pathogens and their compositions. Significant variations in microbiota composition were found between stray and pet dogs, and putative prokaryotic and eukaryotic pathogens were identified. The most prevalent putative bacterial pathogens were Fusobacterium, Helicobacter, and Campylobacter. The most prevalent putative eukaryotic pathogens were Giardia, Pentatrichomonas, and Cystoisospora. Interestingly, Campylobacter, Giardia, and Pentatrichomonas were found to be significantly more prevalent in stray dogs than in pet dogs. The variation in the prevalence of potential pathogens in dog feces could be attributed to environmental factors, including dietary variances and interactions with wildlife, particularly in stray dogs. These factors likely contributed to the observed differences in pathogen occurrence between stray and pet dogs. This study offers valuable insights into the zoonotic risks associated with dog populations residing in diverse environments. By identifying and characterizing putative pathogens in dog feces, this research provides essential information on the impact of habitat on dog-associated pathogens, highlighting the importance of public health planning and zoonotic risk management.

Environmental contamination with feces of free-roaming dogs and the risk of transmission of Echinococcus and Taenia species in urban regions of southeastern Iran

BackgroundDogs are the most important definitive hosts of zoonotic taeniid helminths worldwide. Different Echinococcus and Taenia species of domestic and wild carnivores pose a potential risk to human population. High populations of free-roaming dogs (FRDs) in urban areas of Iran and widespread contamination of the environment with dog feces is a potential source of infecting people living in the urban regions with cystic echinococcosis (CE). Our knowledge on the risk of CE transmission in the urban settings in the endemic regions is limited. The present study surveyed the species and genotypes of E. granulosus sensu lato and other taeniids by examining feces of free-roaming dogs in the urban areas in the city of Kerman, southeastern Iran.MethodsThe city was divided into 100 consecutive blocks of which 25 blocks were randomly selected. Fecal samples of FRDs were counted, mapped and fresh samples were collected. Then Zinc chloride flotation, and sequential sieving was performed, and the samples were examined under an inverted microscope. Single individual taeniid eggs were isolated, partial nad1 gene was amplified and sequenced to identify species and genotypes.ResultsIn total 5607 fecal samples of dogs were mapped and 83 fresh samples were collected. Taeniid eggs were detected in nine fecal samples (10.8%) from seven out of the 25 city blocks (28.0%). Echinococcus eggs were found in four samples (4.8%) from three city blocks, two samples containing E. granulosus sensu stricto (2.4%), two samples containing E. canadensis G6/7 (2.4%). In addition, three samples contained eggs of Taenia hydatigena (3.6%), and one sample of Taenia serialis (1.2%).ConclusionsThis study documented the potential risk of CE transmission to humans resulting from the feces of dogs roaming freely in urban areas.Graphical

Dog as a part of ritual space in Przeworsk culture settlements: A zooarchaeological case study of Sławsko Wielkie, Poland

So-called “dog burials” represent a common phenomenon discovered on many sites dating to the late Iron Age in Central Europe. The article focuses on zooarchaeological analysis of skeletal dog remains from Sławsko Wielkie, site 12 (Poland), a settlement of Przeworsk culture, dated to the La Tène and Roman periods (3rd century BCE – 5th century CE). Although these deposits were often discussed in the older archaeological literature regarding the ritual sphere of Iron Age societies, they still lack more systematic zooarchaeological research. In this study, we present the results of the research focused on morphotype and life conditions of the Iron Age dog population from the Polish Lowlands. A broad spectrum of methods is proposed, including biometry, age of death and sex estimations, observations of pathological alterations, and contextual and comparative analyses supplemented with radiocarbon dating.

The utilization of the Vezzoni modified Badertscher distension device in breeding programs: Heritability estimates and effect on the hip dysplasia prevalence.

Canine hip dysplasia (CHD) is a common orthopedic condition, influenced by both genetic and environmental factors. While current breeding programs often rely on ventrodorsal hip-extended (VDHE) radiographs, it is known they fail to accurately assess hip joint laxity. Therefore additional laxity-oriented diagnostic techniques have been developed. This study aims to evaluate the effectiveness of the Vezzoni modified Bädertscher distension device (VMBDD) technique, which quantifies hip joint laxity with the laxity index (LI), as a screening tool in two breeding programs. Data from a Belgian population of assistance dogs (population A) and a French population of guide dogs (population B) were analyzed. The heritability estimates of the LI, estimated using Bayesian statistical methods, were high in both populations (0.83 in population A and 0.82 in population B). Improved screening of parents by combining the VMBDD technique with the VDHE, significantly decreased LI and the prevalence of CHD in their offspring. In population A, when two parents were screened with the VMBDD compared to one, there was an average LI decrease of 0.03 (P<0.05). In population B, when one or both parents were screened with the VMBDD compared to none, the average LI decrease was 0.04 (P< 0.05) and 0.05 (P<0.01), respectively. In population A, screening both parents with the VMBDD in addition to the VDHE, resulted in 82.7% (P< 0.05) lower odds of CHD compared to screening only one parent. In population B, screening one parent led to 72.3% (P<0.05) lower odds of CHD compared to none of the parents being screened. In population B, when both parents were screened with the VMBDD, not a single case of CHD was observed in the puppies. In conclusion, based on these results, the VMBDD technique has the potential to drastically reduce CHD prevalence and is as such an excellent tool for breeding programs.

In sickness and health - a questionnaire based study regarding immune mediated diseases and neoplasia in Swedish Nova Scotia Duck Tolling Retrievers

BackgroundThe Nova Scotia Duck Tolling Retriever (NSDTR) has previously been highlighted as a breed at risk for developing immune mediated diseases and cancer. The immune response is of great importance for the development of neoplastic disease and a dysregulated immune response may predispose to cancer. Two of the commonly seen immune mediated diseases in NSDTRs are immune mediated rheumatic disease (IMRD), which bears similarities to systemic lupus erythematosus (SLE) affecting humans, and steroid-responsive meningitis-arteritis (SRMA), which is a non-infectious inflammation of the meninges and the leptomeningeal vessels. The aim of this survey study was to investigate the lifetime prevalence of immune mediated diseases and tumors among Swedish NSDTRs based on owners’ information. The study design was cross-sectional. A questionnaire was sent to 4102 persons who owned or had previously owned a NSDTR. The questions concerned information about the dog and its overall health status as well as specific diseases.ResultsThe response rate was 30%, including 935 live NSDTRs, corresponding to approximately 20% of the current population registered in Sweden (n = 4564), and 177 dead dogs. The surveyed dogs were spread over different ages and sex and corresponded to the typical demographic profile of the general dog population. Of the 935 individuals that were alive, 28 dogs (3%) were reported as previously diagnosed with IMRD and 33 dogs (3.5%) were reported as previously diagnosed with SRMA, one dog was reported to have been diagnosed with both SRMA and IMRD. There were 129 dogs (14%) reported to have or have had a neoplasia of some kind. For the dead dogs (n = 177), almost 40% of the owners reported neoplasia as the main reason for death/euthanasia.ConclusionThis study reports an estimated lifetime prevalence of IMRD and SRMA, in the studied population of Swedish NSDTRs, of 3.0 and 3.5% respectively. In this study, 14% of the living dogs (n = 935) were reported to have a neoplasia of some kind and almost 40% of the deceased dogs (n = 177) were euthanized due to neoplasia or suspicion of it.

Dog and owner demographics impact dietary choices in Dog Aging Project cohort.

To describe the demographic factors of owners and their dogs associated with owner feeding choices and the regularity with which those diets were fed to a US-based population of dogs. This cross-sectional analysis examined 40,367 initial survey responses from US dog owners participating in the Dog Aging Project. The surveys were collected from January 2, 2020, to December 31, 2022, and included primary and secondary diet component types and dog and owner demographic variables. Each demographic variable was compared across diet type choices with a χ2 test of independence. Most owners (82%) fed a commercially prepared extruded dry diet (kibble) as the primary diet component. Most owners (89%) reported that they fed their dogs a consistent diet over time. Owner demographic factors (income, education level) were less correlated with difference in diet choices than dog demographic factors (size, neuter status, purebred status, activity level), but owner age did correlate with choice: younger owners tended to feed kibble more compared to older owners. Home-cooked diets were most often consumed by small (< 30-lb) dogs, purebred dogs in poorer health status, and dogs with owners aged 45 years or older. Raw diets were more commonly fed to purebred, intact, and highly active dogs. Ten percent of service dogs were reportedly fed a raw diet of some sort. Demographic variables are associated with statistically significant differences in diet types selected. Nutrition studies examining health outcomes associated with the feeding of different diet types should account for these factors during design or analysis in order to avoid bias. An epidemiological tool, the directed acyclic graph, is presented. This information will help clinicians in their discussions with clients about pet nutrition.

Tumor Grade and Mitotic Count Are Prognostic for Dogs with Cutaneous Mast Cell Tumors Treated with Surgery and Adjuvant or Neoadjuvant Vinblastine Chemotherapy.

Canine cutaneous mast cell tumors (cMCTs) have variable rates of recurrence and metastasis. We evaluated how various prognostic factors affect survival, recurrence, and metastasis in dogs with cMCT who underwent surgery and vinblastine chemotherapy. 90 dogs with cMCT treated with surgery and vinblastine at a veterinary referral institution were included. Medical records were retrospectively reviewed. Prognostic factors were evaluated. Most dogs (94%) had grade 2 or 3 cMCTs. Neoadjuvant vinblastine was used in 18 dogs, and none progressed locally before surgery. The use of neoadjuvant vinblastine was associated with a higher chance of local recurrence (p = 0.03) but not survival. Shorter survival times were found for tumors that were high-grade (p < 0.001), grade 3 (p < 0.001), or a MC of >5 (p < 0.001). Dogs with grade 2 tumors that were low-grade lived longer than those with high-grade tumors (p < 0.001). Histologic tumor-free margins and the ability to achieve local tumor control were not associated with outcome. Both grading systems and MC were prognostic for survival in this population of dogs, supporting the need for the standard reporting of histopathologic findings. Neoadjuvant chemotherapy can be effective in downsizing cMCTs but does not influence survival. These findings are consistent with previous publications, showing the benefits of a more modern population of patients, surgical treatments, and histopathologic assessments.

The role of human behaviour change in companion animal management: A case study of Praia de Faro, Portugal

Abstract Humane dog and cat population management has relied on Trap, Neuter, Release (TNR) projects in an attempt to control or reduce free-roaming populations and improve the welfare of dogs and cats. It has been recognised that the root cause of dog and cat population and welfare issues is people and their attitudes and behaviours towards dogs and cats – whether this is through lack of responsible ownership leading to loss or abandonment, or programs that focus on single tools such as sterilisation. Failing to recognise the interdependency that exists between individual and community responsibility means that projects that rely solely on the actions of an individual or a small group, and do not involve the wider community, risk limiting their impact and sustainability. Typically, sterilisation projects regularly fail to address this issue. The concept of human behaviour change is beginning to be used in dog population management programs but is still largely unused and untested in cat population management programs. In 2013, a 4-year project in Praia de Faro, Portugal, started to explore how communities respond to a local program using community engagement tools to measure and evaluate changes in attitudes and behaviours towards owned and un-owned dogs and cats. The research question is: ‘does community involvement and participation in a project increase its chances of success in relation to improved welfare for animals?’ Results suggest improved dog and cat welfare, positive changes in attitudes and behaviours of the community, and increased community involvement.

INVENTARISASI VEGETASI KUNCI YANG MENDUKUNG KEBERADAAN SATWA DI BLOK PEMANFAATAN TAMAN BURU MASIGIT KAREUMBI

Taman Buru Masigit Kareumbi is a conservation forest area serving as a habitat for various wildlife species in West Java. This area is dedicated to regulated hunting to manage the populations of Timor deer, muntjac, wild dogs, leopards, jungle cats, wild chickens, slow lorises, binturongs, monkeys, langurs, and hornbills. The presence of these species indicates the need for conservation efforts in TB Masigit Kareumbi to maintain the biodiversity of its flora and fauna and preserve the ecological functions of the forest. Research methods included a vegetation survey conducted from May to August 2023 across seven trails within the Utilization Block of TB Masigit Kareumbi. The study identified various vegetation types along these seven different trails, which play a crucial role as habitat and food sources for other game species. These include: rasamala trees, saint trees, puspa trees, are trees, kendang trees, and grassland vegetation such as alang-alang. The potential game species supported by these types of vegetation are: primates and birds that inhabit and forage on rasamala, puspa, and saninten trees; civets that feed on aren trees; porcupines that forage on kondang trees; and alang-alang grass which provides food for deer and habitat for reptiles. This study demonstrates that the Utilization Block of TB Masigit Kareumbi is a forest area with a plant species composition that continues supporting wildlife's survival and presence. Thus, the updated information from this research will assist the managers of TB Masigit Kareumbi in maintaining the balance and preservation of the existing vegetation species.