Rate Of Division Research Articles

Does the TruBlue Laser Set Microlaryngoscopy Equipment on Fire? A Systematic Evaluation.

The risk of fire during laser microlaryngoscopy is well known. However, limited information is available about fire risk with the novel TruBlue laser. This study systematically evaluates its interactions with common surgical supplies, offering valuable insights into safety considerations for surgeons. We used experimental conditions to test the extent to which TruBlue laser energy produces smoke, perforation, or fire in Rüsch®, Medtronic™ and microlaryngeal endotracheal tubes and in surgical pledgets. Only the Microlaryngeal Tube (MLT) caught fire. Notably, it happened only when the laser fiber shifted on the tube's surface. Smoke emerged solely from the laser fiber applied to the Medtronic™ shaft and only during continuous contact mode. Cuff perforation and smoke emanating from the shaft occurred in three-quarters of the Rüsch® trials. The pledgets' radiopaque segment exhibited a greater combustibility than other segments (p < 0.01). In many of the pledget trials, faster smoke emission occurred with shorter laser-to-target distances (p < 0.05). Water-soaked pledgets displayed a reduced rate of smoke production (p < 0.01) and string division. The Medtronic™ tube assures remarkable safety with a nonignitable shaft and low cuff ignition. The MLT poses the highest ignition risk. Cuff perforation risk is mitigated by maintaining a 0.3-cm distance from the laser fiber tip. Pledget fire risk is mitigated by positioning the radiopaque part away from the laser beam and by soaking the pledget with water. Laser division of the pledgets' string was common. NA Laryngoscope, 2024.

Distinct interspecies thermal resistance strategies exhibited by euplanktonic, tychoplanktonic and benthic diatoms under marine heatwaves

Extreme climate events, such as marine heatwaves (MHWs), are expected to occur more frequently and intensely in the future, resulting in a substantial impact on marine life. The way that diatoms respond to MHWs may have crucial effects on global primary production and biogeochemical cycles. Euplanktonic diatoms appear to benefit from MHWs directly, but this phenomenon needs an explanation. As concerns tychoplanktonic and benthic diatoms, no studies have been addressed on their thermal response strategies. To address this, we investigated the responses and underlying mechanisms of three typical growth forms of diatoms, Pseudo-nitzschia multiseries (euplanktonic), Paralia guyana (tychoplanktonic) and Navicula avium (benthic), under heat stress by combining a growth experiment with transcriptomic analysis. Our results showed that the physiological responses of diatoms to MHWs and underlying molecular mechanisms are largely related to their growth forms. The euplanktonic diatom was first depressed, but then had a distinct increase in the growth rate accompanied by inducing zeatin and unsaturated fatty acid biosynthesis and repressing substance assimilation and energy metabolism. Contrarily, the benthic diatom showed elevated substance and energy demands for macromolecules accumulation by reducing cell division and increasing photosynthesis and nitrogen assimilation. The tychoplanktonic diatom exhibited higher physiological plasticity to maintain growth and cellular homeostasis. Our results indicate the increased rate of cell division in euplanktonic diatoms under heat stress is likely an emergency response strategy promoting diatom dispersal for survival, but at the cost of disturbances of metabolic balance.

Impact of gibberellic acid GA3, quantum dot biochar, and rhizosphere bacteria on fenugreek plant growth and stress responses under lead stress

Lead (Pb) is a stress that can cause problems with several aspects of a plant’s metabolism, potentially impeding the plant’s ability to grow and develop. The use of gibberellic acid (GA3), quantum dot biochar (QDBC), and rhizobacteria (RB) can be effective methods to overcome this problem. Gibberellic acid is a crucial plant hormone that regulates plant growth, cell division, tissue differentiation, flowering, photosynthesis, and transpiration rate. It also significantly impacts crop resilience to stress, affecting plant morphology, enzymatic activity, and physiology. Biochar, a soil supplement, enhances plant development soil health, and reduces stress effects. Due to its large surface area and porosity, it increases soil water-holding capacity, nutrient retention, and microbial activity. Quantum dots, semiconductor nanoparticles, have been proposed as a potential method to alleviate plant stress by acting as antioxidants, reducing oxidative stress, and controlling nutrient and growth regulators. Rhizobacteria, soil bacteria in plant roots, stimulate plant growth, nutrient absorption, and harvesting capacity. They produce phytohormones, increase mineral and nitrogen accessibility, and can induce systemic resistance (ISR), affecting plant defense. This study investigates the effects of combining GA3, QDBC, and RB as amendments to fenugreek, both with 500 Pb stress and without Pb stress. Treatments (control, 0.25 GA3mg/L-QDBC, 0.5 GA3mg/L-QDBC, 0.25 GA3mg/L-QDBC + RB, and 0.5 GA3mg/L-QDBC + RB) were applied in six replications using a completely randomized design. Results demonstrate that the combination of 0.5 GA3mg/L-QDBC + RB with 500 Pb stress led to significant enhancements in fenugreek shoot fresh weight (15.62%), root fresh weight (73.53%), shoot dry weight (24.00%), and root dry weight (36.53%) compared to the control. Additionally, there were notable improvements in chlorophyll a (57.23%), chlorophyll b (19.21%), and total chlorophyll (36.23%) compared to the control under Pb stress, also showing the potential of 0.5 GA3mg/L-QDBC + RB with 500 Pb stress. The study suggests that combining 0.5 GA3mg/L-QDBC + RB with 500 Pb stress can effectively mitigate Pb stress in fenugreek.

Assessment of the Biocontrol Efficacy of Silver Nanoparticles Synthesized by Trichoderma asperellum Against Infected Hordeum vulgare L. Germination

This study investigated the biosynthesis, statistical optimization, characterization, and biocontrol activity of silver nanoparticles (AgNPs) produced by newly isolated Trichoderma sp. The Trichoderma asperellum strain TA-3N was identified based on the ITS gene sequence, together with its phenotypic characteristics (GenBank accession number: OM321439). The color change from light yellow to brown after the incubation period indicates AgNPs biosynthesis. The UV spectrum revealed a single peak with the maximum absorption at 453 nm, indicating that T. asperellum produces AgNPs effectively. A Rotatable Central Composite Design (RCCD) was used to optimize the biosynthesis of AgNPs using the aqueous mycelial-free filtrate of T. asperellum. The optimal conditions for maximum AgNPs biosynthesis (156.02 µg/mL) were predicted theoretically using the desirability function tool and verified experimentally. The highest biosynthetic produced AgNPs by T. asperellum reached 160.3 µg/mL using AgNO3 concentration of 2 mM/mL, initial pH level of 6, incubation time of 60 h, and biomass weight of 6 g/100 mL water. SEM and TEM imaging revealed uniform spherical shape particles that varied in size between 8.17 and 17.74 nm. The synthesized AgNPs have a Zeta potential value of −9.51 mV. FTIR analysis provided insights into the surface composition of AgNPs, identifying various functional groups such as N–H, -OH, C-H, C=O, and the amide I bond in proteins. Cytotoxicity and genotoxicity assays demonstrated that AgNPs in combination with T. asperellum can mitigate the toxic effects of Fusarium oxysporum on barley. This intervention markedly enhanced cell division rates and decreased chromosomal irregularities. The results indicate that AgNPs synthesized by T. asperellum show the potential as an eco-friendly and efficient method for controlling plant diseases. Further studies are necessary to investigate their possible use in the agricultural sector.

The Success Rate of Cryopreservation and Thawing of Embryos at Day 3 and Day 5 Following Intracytoplasmic Sperm Injection (ICSI)

Cryopreservation, the technique of freezing and thawing embryos, is essential in in vitro fertilization (IVF) treatments to ensure the long-term preservation of embryos for future use. This innovative study evaluates the effectiveness of these procedures conducted at a private hospital in Tangerang, Indonesia, over a period of seven years (2016-2023), involving 18,000 couples. The design research was conducted retrospectively. The study examined embryos that underwent cryopreservation and subsequent thawing, with data collected on the rates of cell division observed on Day 3 and the rates of blastocyst formation on Day 5 after thawing. The study investigated the rates of cellular division on Day 3 and the development of blastocysts on Day 5 following the thawing process. The findings demonstrated a remarkable cleavage rate of 95.24% and a blastocyst rate of 97.22%. The remarkable results underscore the efficacy of the advanced freezing and thawing techniques employed. This discovery is highly significant since there is an urgent requirement to improve the success rates of in vitro fertilization (IVF) for couples facing infertility. It offers a fresh sense of hope and increased opportunities for establishing successful pregnancies with the improvement of cryopreservation techniques.

Asymmetric stem cell division maintains genetic heterogeneity of tissue cells.

Within a given tissue, the stem cell niche provides the microenvironment for stem cells suitable for their self-renewal. Conceptually, the niche space constrains the size of a stem-cell pool, as the cells sharing the niche compete for its space. It has been suggested that either neutral- or non-neutral-competition of stem cells changes the clone dynamics of stem cells. Theoretically, if the rate of asymmetric division is high, the stem cell competition is limited, thus suppressing clonal expansion. However, the effects of asymmetric division on clone dynamics have never been experimentally tested. Here, using the Drosophila germline stem cell (GSC) system, as a simple model of the in-vivo niche, we examine the effect of division modes (asymmetric or symmetric) on clonal dynamics by combining experimental approaches with mathematical modeling. Our results suggest that the rate of asymmetric division positively correlates with the time a stem cell clone takes to expand. Taken together, our data suggests that asymmetric division is essential for maintaining the genetic variation of stem cells and thus serves as a critical mechanism for safeguarding fertility over the animal age or preventing multiple disorders caused by the clonal expansion of stem cells.

The rate of stem cell division decreases with age

The rate of stem cell division decreases with age

The Performance of Fixed Step Size and Adaptive Step Size Numerical Methods for Solving Deterministic Cell-Growth Models

Deterministic cell-growth models describe the growth of cell populations using fixed mathematical rules, assuming no randomness in the system. These models are often based on differential equations that account for the rates of cell division, death, and other biological processes. The solution to the system is obtained via numerical methods. Most of the developed approaches are based on fixed step sizes. However, fixed step size implementation failed to offer the optimal solutions when dealing with stiff challenges. Fixed step size methods can be unstable for stiff equations, where some components of the solution change much more rapidly than others. The step size, required to maintain stability can become impractically small. Thus, the adaptive step size method is required. Adaptive step size methods adjust the step size dynamically based on the behavior of the solution, aiming to maintain a desired level of accuracy while optimizing computational efficiency. These methods are particularly useful for solving ordinary differential equations (ODEs) where the solution can vary rapidly in some regions and slowly in others. This study is devoted to comparing the implementation of fixed step size and adaptive step size in solving ordinary differential equations (ODEs). The fixed step size and adaptive step size numerical method are solved in this study via the fourth order Runge-Kutta method (RK4) and Runge-Kutta Fehlberg 45 method (RKF45). The performance of both numerical methods used will be analyzed by comparing the numerical results approximated with the actual data. Subsequently, the absolute error, relative error, and rounding-off error will be calculated to compare both approaches. Based on the more precise findings, this work has shown that adaptive step size is predicted to be the optimal representation for solving ODEs. As a result, this may help mathematicians to choose the most effective numerical approach for solving ODEs.

Survival Resonances during Fractional Killing of Cell Populations.

Fractional killing in response to drugs is a hallmark of nongenetic cellular heterogeneity. Yet how individual lineages evade drug treatment, as observed in bacteria and cancer cells, is not quantitatively understood. We study a stochastic population model with age-dependent division and death rates, allowing for persistence. In periodic drug environments, we discover peaks in the survival probabilities at division or death times that are multiples of the environment duration. The survival resonances are unseen in unstructured populations and are amplified by persistence.

The dwarf & pale leaf mutation reduces chloroplast numbers, resulting in sugar depletion that inhibits leaf growth of maize seedlings

Plant growth is ultimately driven by cell division and expansion, but how these processes are regulated to mediate a wide range of genotypic variation in organ size is still poorly understood. To address this, we screened an EMS maize mutant population to identify a new EMS maize dwarf mutant with small, pale-yellow leaves (dpl). The mutation was mapped to a region of 11.58 Mb at the 3’ end of chromosome 7. We identified Zm00001d022394 as a potential causal gene for the dpl phenotype, encoding a pentatricopeptide repeat-containing (PPR) family protein involved in chloroplast gene expression and function, explaining the pale color of dpl. Mature dpl leaves are thinner and shorter due to a reduced number of cells of approximately normal length. The chloroplasts of dpl are reduced in size and number, correlating with a decreased chlorophyll content, however chloroplast ultrastructure was not affected. Consistent with the reduced chlorophyll content photosynthetic rate of dpl were reduced by 50 % and a 30 reduction of Fv/Fm suggests photoinhibition. As a consequence, soluble and insoluble sugar levels are severely reduced throughout the leaf growth zone. At the cell level reduced cell division rates and size of the division zone, explain the reduced leaf elongation rate (LER). The growth of dpl leaves can be restored by supplying growing leaves with sucrose through their cut tips, which also restores sucrose levels in the division zone of maize leaf, demonstrating that limited sugar availability explains the reduced growth phenotype. Inversely, we phenocopied the mutant growth phenotype by inhibiting photosynthetic electron transport in wild type plants with DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea). Our study of dpl provides a functional link between inhibition of photosynthesis, soluble sugar flux to the leaf growth zone, the regulation of cell division and whole leaf growth.

Expression of carbohydrate-related genes underlying 3,5,6-TPA-induced fruitlet abscission in citrus

In citrus, the synthetic auxin 3,5,6-trichloro-2-pyridyloxyacetic acid (3,5,6-TPA), applied as a foliar spray at a concentration of 15 mg l− 1 during physiological fruitlet abscission, caused additional fruitlet drop and reduced the number of fruits reaching maturity. The effect was much more pronounced at full physiological abscission than after. In this study, this thinning effect was successfully exploited for the first time in sour orange trees grown in an urban environment, reducing harvesting costs by up to almost 40%. This effect is mediated by the leaves, which alter their photosynthetic activity. Our results show a reduction of carbon fixation and sucrose synthesis in the leaf, by 3,5,6-TPA repression of the RbcS, SUS1 and SUSA genes, its transport to the fruit, as shown by the reduced expression of the sucrose transporter genes SUT3 and SUT4, and its hydrolysis in the fruit, mainly by repression of the SUS1 gene expression. Genes involved in auxin homeostasis in the fruit, TRN2 and PIN1, were also repressed. The coordinated repression of all these genes is consistent with the decrease in the fruit cell division rate, as shown by the repression of CYCA1-1 gene, leading to the production of ethylene, which ultimately induces fruitlet abscission.

The completing of the second meiotic division by MII mouse oocytes correlates with the positioning of F-actin and mitochondria in the ooplasm

Actin filaments play an essential role in the process of oocyte maturation and completion of meiosis. However, whether the localization of F-actin in the ooplasm is associated with normal completion of the second meiotic division remains unclear. Mitochondrial distribution is another important parameter correlating directly with MII oocyte capacity to finalize meiosis. Our objective was to examine the role of actin microfilaments in the distribution of mitochondria and, respectively, Metaphase II (MII) oocytes meiotic potential. We show monoclonal antibody-mediated inhibition of actin polymerization in young mouse oocytes, reduction of the amount of F-actin, and induction of mitochondrial clustering induced by antibody treatment. Similar phenotype, even in untreated eggs, was observed in in vitro oocyte aging experiments. Observed changes correlate with reduced ability of MII oocytes to extrude the second polar body and form the pronuclei. Changes in colocalization of F-actin and mitochondria likely resulted from disturbed cytoskeleton architecture. The perturbations in the amount of F-actin and its distribution largely coincide with mitochondrial redistribution. Based on these data, we suggest actin microfilament’s participation in redistribution of mitochondria during MII oocyte aging in vitro. Accordingly, patterning of F-actin is indicative of high rate of the completed second meiotic division. These results help evaluating oocyte’s quality and choosing optimal time between placement into culture and in vitro fertilization.

The cyanobacterial circadian clock couples to pulsatile processes using pulse amplitude modulation.

Cellular processes are dynamic and often oscillatory, requiring precise coordination for optimal cell function.1,2,3,4,5,6,7 How distinct oscillatory processes can couple within a single cell remains an open question. Here, we use the cyanobacterial circadian clock8,9 as a model system to explore the coupling of oscillatory and pulsatile gene circuits. The cyanobacterial circadian clock generates 24-h oscillations in downstream targets10,11,12,13,14,15 to time processes across the day/night cycle.9,16,17,18,19,20,21,22 This timing is partly mediated by the clock's modulation of the activity of alternative sigma factors,14,23,24,25 which direct RNA polymerase to specific promoters.26 Using single-cell time-lapse microscopy and modeling, we find that the clock modulates the amplitude of expression pulses of the alternative sigma factor RpoD4, which occurs only at cell division. This pulse amplitude modulation (PAM), analogous to AM regulation in radio transmission,27 allows the clock to robustly generate a 24-h rhythm in rpoD4 expression despite rpoD4's pulsing frequency being non-circadian. By modulating cell division rates, we find that, as predicted by our model, PAM regulation generates the same 24-h period in rpoD4 pulse amplitude over a range of rpoD4 pulse frequencies. Furthermore, we identify a functional significance of rpoD4 expression levels: deletion of rpoD4 results in smaller cell sizes, whereas an increase in rpoD4 expression leads to larger cell sizes in a dose-dependent manner. Thus, our work reveals a link between the cell cycle, clock, and RpoD4 in cyanobacteria and suggests that PAM regulation can be a general mechanism for biological clocks to robustly modulate pulsatile downstream processes.

Ин витро култивиране на говежди ембриони

Embryo cultivation is the longest step during the in vitro embryo production process. In this study, the effect of adding 5% FCS or 3 mg/ml BSA to SOF and KSOM in vitro culture media was investigated. When adding FCS in SOF medium, a better effect on separation was observed (P &lt; 0.05), and the percentage of blastocysts was higher - 58.6%. When BSA was added to SOF medium, the percentage of blastocysts was 40.1% - significantly lower. When cultured in KSOM medium with the addition of FCS, the division rate was lower than when cultured in SOF+FCS and the proportion of blastocysts was also slightly lower. When BSA was added to the KSOM medium, the percentage of blastocysts was almost as in the SOF+BSA group. Аt the conclusion of the conducted experiment, the addition of FCS in KSOM and SOF media is beneficial for in vitro cultivation, resulting in a higher percentage of blastocysts.

Current Insights into Therapeutic Potential of Terpenoids as Anticancer Agents.

Cancer is regarded as one of the main causes of death globally. Future predictions indicate that the death rate from cancer will keep rising, which may reach 11.4 million in 2030. Carcinogenesis refers to the phenomenon of transforming a normal cell into a cancer cell. Cancer is characterized by unregulated and uncontrolled cell division due to alterations at the molecular and genetic levels. Gene mutations can speed up the rate of cell division, which leads to cancer. Metastasis entails the dissemination of cancer cells from the primary site to distant regions of the body via the circulatory or lymphatic systems. This review is mainly focusing on the anticancer properties of terpenoids. In the case of human beings, several types of cancers can be treated clinically based on the form and phase of the cancer. So, there are different types of treatment regimens available for the management of cancer, such as immunotherapy, hormonal therapy, radiation therapy, and chemotherapy. Several problems are associated with cancer therapy, including chemoresistance, severe toxicity, relapse, and metastasis. To minimize these complications, natural products like terpenoids seem to be beneficial for the effective management of cancer. Experimental results revealed that the anticancer potential of terpenoids is due to activation of apoptosis and stimulation of cell cycle arrest. Some of the terpenoids exhibit anticancer effects by inhibiting angiogenesis and metastasis via the regulation of several signaling pathways intracellularly. Certain terpenoids have been shown to work in concert with anticancer medications (doxorubicin, cisplatin, paclitaxel, and 5-fluorouracil) to provide synergistic effects. These terpenoids have also been shown to be effective against cancer cells that are resistant to several drug therapies. The current study will focus on signaling pathways and mode of action of several types of terpenoids as anticancer agents. Further, it will provide insights into the ongoing clinical trials and prospective pathways for the advancement of terpenoids as possible anti-cancer agents.

Glutathione accelerates the cell cycle and cellular reprogramming in plant regeneration.

The plasticity of plant cells underlies their wide capacity to regenerate, with increasing evidence in plants and animals implicating cell cycle dynamics in cellular reprogramming. To investigate the cell cycle during cellular reprogramming, we developed a comprehensive set of cell cycle phase markers in the Arabidopsis root. Using single-cell RNA-seq profiles and live imaging during regeneration, we found that a subset of cells near an ablation injury dramatically increases division rate by truncating G1. Cells in G1 undergo a transient nuclear peak of glutathione (GSH) prior to coordinated entry into S phase followed by rapid divisions and cellular reprogramming. A symplastic block of the ground tissue impairs regeneration, which is rescued by exogenous GSH. We propose a model in which GSH from the outer tissues is released upon injury licensing an exit from G1 near the wound to induce rapid cell division and reprogramming.

Rapid growth rate of Enterobacter sp. SM3 determined using several methods

BackgroundBacterial growth rate, commonly reported in terms of doubling time, is frequently determined by one of two techniques: either by measuring optical absorption of a growing culture or by taking samples at different times during their growth phase, diluting them, spreading them on agar plates, incubating them, and counting the colonies that form. Both techniques require measurements of multiple repeats, as well careful assessment of reproducibility and consistency. Existing literature using either technique gives a wide range of growth rate values for even the most extensively studied species of bacteria, such as Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. This work aims to apply several methods to reliably determine the growth rate of a recently identified species of Enterobacteriaceae, called Enterobacter sp. SM3, and to compare that rate with that of a well-known wildtype E. coli strain KP437.ResultsWe extend conventional optical density (OD) measurements to determine the growth rate of Enterobacter sp. SM3. To assess the reliability of this technique, we compare growth rates obtained by fitting the OD data to exponential growth, applying a relative density method, and measuring shifts in OD curves following set factors of dilution. The main source of error in applying the OD technique is due to the reliance on an exponential growth phase with a short span. With proper choice of parameter range, however, we show that these three methods yield consistent results. We also measured the SM3 division rate by counting colony-forming units (CFU) versus time, yielding results consistent with the OD measurements. In lysogeny broth at 37oC, SM3 divides every 21 ± 3 min, notably faster than the RP437 strain of E. coli, which divides every 29 ± 2 min.ConclusionThe main conclusion of this report is that conventional optical density (OD) measurements and the colony-forming units (CFU) method can yield consistent values of bacterial growth rate. However, to ensure the reproducibility and reliability of the measured growth rate of each bacterial strain, different methods ought to be applied in close comparison. The effort of checking for consistency among multiple techniques, as we have done in this study, is necessary to avoid reporting variable values of doubling time for particular species or strains of bacteria, as seen in the literature.

Predator-induced defense decreases growth rate and photoprotective capacity in a nitrogen-limited dinoflagellate, Alexandrium minutum

Some dinoflagellates produce toxic secondary metabolites that correlate with increased resistance to grazers. The allocation costs of toxin production have been repeatedly addressed, but with conflicting results. Few studies have considered the potential costs of this defense to the photosystem, even though defense toxins (e.g., karlotoxins and brevetoxins) are closely linked to the photoprotective process. Here, we used chemical cues from copepods to induce paralytic shellfish toxin (PST) production in resource-limited Alexandrium minutum and quantitatively determined the growth rate and potential trade-offs with the photosystem process. The results show that grazer-induced, more toxic A. minutum had larger cell volume, lower cell division rate, and lower pigment content under nitrogen-limited conditions than control cells. In addition, predator cues led to a lower relative abundance of photoprotective xanthophylls and a reduced de-epoxidation efficiency of the xanthophyll cycle under high light conditions, reducing the ability of the cells to resist photodamage. Decreased photoprotective capacity may reflect an overlooked defense cost of toxin production.

Small-Scale Biophysical Interactions and Dinophysis Blooms: Case Study in a Strongly Stratified Chilean Fjord

Diarrhetic shellfish poisoning (DSP) toxins and pectenotoxins (PTXs) produced by endemic species of Dinophysis, mainly D. acuta and D. acuminata, threaten public health and negatively impact the shellfish industry worldwide. Despite their socioeconomic impact, research on the environmental drivers of DSP outbreaks in the Chilean fjords is scanty. From 22 to 24 March 2017, high spatial–temporal resolution measurements taken in Puyuhuapi Fjord (Northern Patagonia) illustrated the short-term (hours, days) response of the main phytoplankton functional groups (diatoms and dinoflagellates including toxic Dinophysis species) to changes in water column structure. Results presented here highlight the almost instantaneous coupling between time–depth variation in density gradients, vertical shifts of the subsurface chlorophyll maximum, and its evolution to a buoyancy-driven thin layer (TL) of diatoms just below the pycnocline the first day. A second shallower TL of dinoflagellates, including Dinophysis acuta, was formed on the second day in a low-turbulence lens in the upper part of the pycnocline, co-occurring with the TL of diatoms. Estimates of in situ division rates of Dinophysis showed a moderate growth maximum, which did not coincide with the cell density max. This suggests that increased cell numbers resulted from cell entrainment of off-fjord populations combined with in situ growth. Toxin profiles of the net tow analyses mirrored the dominance of D. acuminata/D. acuta at the beginning/end of the sampling period. This paper provides information about biophysical interactions of phytoplankton, with a focus on Dinophysis species in a strongly stratified Patagonian fjord. Understanding these interactions is crucial to improv predictive models and early warning systems for toxic HABs in stratified systems.

Dissecting dormancy and quiescence in hematopoietic stem cells

Quiescence is a fundamental state of adult hematopoietic stem cells (HSCs) characterized by their residence in the G0 phase of the cell cycle. Despite being quiescent, HSCs retain their capacities for self-renewal and multipotency, enabling them to produce all blood lineages. Recent discoveries have shown that HSCs can dive into an even deeper state of quiescence with a very low division rate in steady-state conditions, known as dormancy. Dormant HSCs (dHSCs) have the most superior stem cell properties among HSCs, placing them at the top of the hematopoietic hierarchy. In this review, we argue that quiescence and dormancy are not synonyms in the context of HSCs. Specifically, dHSCs constitute a unique reserve pool of HSCs, mobilized only under stress conditions to protect the HSC compartment throughout life. While HSC quiescence is well-studied, the molecular features of HSC dormancy remain less well-defined. We will discuss the available methods for dHSC isolation and summarize the latest findings on the roles of niche factors, transcription factors, chromatin regulators, and cell cycle-related proteins in maintaining HSC dormancy. Additionally, we will explore whether insights from the quiescent HSC research can be applied to dHSCs. Lastly, we will assess the therapeutic potential of utilizing or targeting dHSCs to improve stem cell transplantation outcomes and treat hematological diseases, opening new avenues for research and clinical applications in regenerative medicine and oncology.