Diversity Of Fruit Flies Research Articles

Species Diversity of Anastrepha Fruit Flies (Diptera: Tephritidae) in Environments with Different Levels of Anthropogenic Changes.

Studies on the diversity of Anastrepha species have been conducted mainly in agricultural orchards due to the economic importance of this insect group, consequently, research on fruit flies in undisturbed environments is scarce. Moreover, very few studies have investigated the diversity of fruit flies in urban areas, where fruit flies survive under native or exotic hosts. Thus, to understand the diversity of Anastrepha species in an environment with different anthropization degrees, surveys were carried out in four areas of the "Luiz de Queiroz" campus, in Piracicaba, São Paulo State (Brazil), using Multilure traps with putrescine and ammonium acetate for 12 months. The software R 3.6.3 was used to characterize the fruit fly community and the faunistic analysis was carried out using the ANAFAU program. The analysis of species richness and total abundance of fruit flies among the sites was carried out using the rarefaction method and adjustment to the generalized linear model (GLM) using the Poisson distribution, respectively. We collected 1,080 females and 14 species of Anastrepha in the four sampled sites. Anastrepha fraterculus and A. obliqua were the predominant species (behavioral patterns). Similar to agricultural sites, A. fraterculus and A. obliqua also show a predominant occurrence in urban environments.

Species composition, richness, and diversity of fruit flies collected from mango orchards in Saharanpur, Uttar Pradesh, India

Species composition, richness, and diversity of fruit flies collected from mango orchards in Saharanpur, Uttar Pradesh, India

Anna Karenina as a promoter of microbial diversity in the cosmopolitan agricultural pest Zeugodacus cucurbitae (Diptera, Tephritidae).

Gut microbial communities are critical in determining the evolutive success of fruit fly phytophagous pests (Diptera, Tephritidae), facilitating their adaptation to suboptimal environmental conditions and to plant allelochemical defences. An important source of variation for the microbial diversity of fruit flies is represented by the crop on which larvae are feeding. However, a "crop effect" is not always the main driver of microbial patterns, and it is often observed in combination with other and less obvious processes. In this work, we aim at verifying if environmental stress and, by extension, changing environmental conditions, can promote microbial diversity in Zeugodacus cucurbitae (Coquillett), a cosmopolitan pest of cucurbit crops. With this objective, 16S rRNA metabarcoding was used to test differences in the microbial profiles of wild fly populations in a large experimental setup in Eastern Central Tanzania. The analysis of 2,973 unique ASV, which were assigned to 22 bacterial phyla, 221 families and 590 putative genera, show that microbial α diversity (as estimated by Abundance Coverage Estimator, Faith's Phylogenetic Diversity, Shannon-Weiner and the Inverse Simpson indexes) as well as β microbial diversity (as estimated by Compositional Data analysis of ASVs and of aggregated genera) significantly change as the species gets closer to its altitudinal limits, in farms where pesticides and agrochemicals are used. Most importantly, the multivariate dispersion of microbial patterns is significantly higher in these stressful environmental conditions thus indicating that Anna Karenina effects contribute to the microbial diversity of Z. cucurbitae. The crop effect was comparably weaker and detected as non-consistent changes across the experimental sites. We speculate that the impressive adaptive potential of polyphagous fruit flies is, at least in part, related to the Anna Karenina principle, which promotes stochastic changes in the microbial diversity of fly populations exposed to suboptimal environmental conditions.

Diversity of Fruit Flies (Diptera: Tephritidae) Attracted by Methyl Eugenol in Carambola and Papaya Orchads in Malang Regency, East Java, Indonesia

Fruit flies (Diptera: Tephritidae) are among the significant pests in horticultural commodities. Methyl eugenol has been widely used for the detection, monitoring, and control of fruit fly worldwide. This study aimed to investigate the diversity of fruit flies attracted by methyl eugenol in carambola and papaya orchards in Malang Regency, East Java, Indonesia. Fruit flies were collected using modified plastic bottle traps, which were transformed into the Steiner trap model and attracted by methyl eugenol. The traps were placed in two study sites: papaya and carambola orchards. The results showed that three species of fruit flies were captured using methyl eugenol in this study: B. dorsalis, B. umbrosa, and B. carambolae. The most predominant species were B. dorsalis (86.02%), followed by B. carambolae (11.26%), and B. umbrosa (2.72%). Bactrocera dorsalis was the dominant species at both study sites.

Effect of natural attractant essential oil from Ocimum gratisimum from Timor Island against fruit flies

Ocimum gratisimum, well-adapted to arid regions in East Nusa Tenggara, has essential oil that attracts fruit flies, pest important affecting crops like mango and jackfruit. This study aims to assess the impact of O. gratisimum essential oil on the diversity of fruit flies on these plants. The essential oil, source from leaves of O. gratisimum, was obtained from the Bioscience Laboratory of the University Nusa Cendana, East Nusa Tenggara, Kupang, Indonesia. It was applied to mango and jackfruit plants in Baumata Village, Taebenu District, Kupang Regency, East Nusa Tenggara. For experiment, total of 0.2 µL of O. gratisimum essential oil was carefully placed on a cotton swab, which was subsequently positioned within traps constructed using 1.5-L mineral bottles. The results of this study indicated that the essential oil extracted from O. gratisimum leaves had the capacity to attract Bacrtocera umbrosa and Bactrocera dorsalis flies when applied on mango plants. In case of jackfruit plants, the traps successfully lured B. umbrosa, B. dorsalis, and Bactrocera musae. This attraction contains methyl eugenol in the oil derived from O. gratisimum.

Species diversity of fruit flies (Bactrocera spp.) in Guava orchards of Sahanpur District of Uttar Pradesh

Species diversity of fruit flies (Bactrocera spp.) in Guava orchards of Sahanpur District of Uttar Pradesh

Species diversity of fruit flies (Bactrocera spp.) in Guava orchards of Sahanpur district of Uttar Pradesh

Species diversity of fruit flies (Bactrocera spp.) in Guava orchards of Sahanpur district of Uttar Pradesh

The species composition, richness and diversity studies of fruit flies captured from the orchard of Nagpur mandarin

The present experiment was conducted to study the diversity of fruit flies in Nagpur mandarin orchard from different agro-ecological zones of Vidarbha region of Maharashtra, India. The fruit flies samples were collected from 18 places falling under 9 agro-ecological zone using methyl eugenol traps. Out of the total collected catches of fruit flies from each zones, Bactrocera dorsalis was found to be the most prominent species followed by Bactrocera zonata. The Z. cucurbitae and B. correcta which occupied third and fourth rank in species composition, respectively. The species viz., Zeugodacus duplicatus and Zeugodacus gavisus recorded very less abundance at all locations. Shannons index of diversity was maximum in Morshi followed by Katol. Simpson index was more at Tiwasa and Achalpur locations which indicated minimum diversity in the population of fruit flies. Higher diversity of fruit flies were recorded in Morshi and Katol followed by Amravati.

Diversity of Fruit Flies on Three Varieties of Mango in Kuningan Regency, West Java, Indonesia

Fruit flies (Bactrocera spp.) are the major pests on mangoes that constrain the efforts to increase the production and quality of mangoes, causing yield losses of up to 100%. Kuningan Regency is one of the largest mango-producing areas in West Java. However, there is no study about the species of fruit fly in the mango plantation at Kuningan Regency, West Java. This study aimed to fruit fly inventory on Gedong Gincu, Arumanis, and Cengkir mangoes. The research used a survey method and was analyzed using descriptive analysis. Purposive sampling was used to determine mango samples by randomly selecting fruits that were attacked by fruit flies from mango fields owned by farmers, wholesalers, and markets in Sukadana Village, Ciawigebang District, Kuningan Regency, West Java. The samples were taken to the Plant Pest Laboratory at Universitas Padjadjaran for incubation till the appearance of imago. The findings showed that the Bactrocera dorsalis complex consisted of B. dorsalis, B. carambolae, and interspecific crosses. The fruit fly diversity index was considerably low (0.21<H‘<0.53). B. dorsalis was determined to be the predominant species in all mango variety and locations, with a value of 95.12%.

Methyl Eugenol (Parapheromone) Trapping System on Diversity of Fruit Flies and Influence of Weather Parameters on Trap Catches in Mango and Guava Cropping Systems

Studies on species diversity and influence of weather parameters on methyl eugenol (parapheromone) trap catches were carried out in mango and guava orchards at Coimbatore and Dindigul Districts of Tamil Nadu from Standard Meteorological Week (SMW) 16th to SMW 25th. Species diversity indices were calculated, and the methyl eugenol trap catches were correlated with the weather parameters. The results revealed that four fruit fly species viz., Oriental fruit fly, Bactrocera dorsalis (Hendel), Guava fruit fly, B. correcta (Bezii), B. caryeae (Kapoor), and Peach fruit fly, B. zonata (Saunders), were attracted to the parapheromone methyl eugenol traps. Among them the population of B. dorsalis was higher in both mango and guava orchards of Coimbatore and Dindigul Districts. The highest species diversity indices for fruit flies viz., Shannon H’ (0.936) and Simpsons D’ (0.593), evenness (0.468), and richness (0.482) were observed in guava orchard located in Coimbatore District, and the minimum diversity indices Shannon H’ (0.254), Simpsons D’ (0.921), evenness (0.160), and richness (0.291), were observed in mango orchard located in Dindigul District. The maximum number of fruit flies were trapped in 19th SMW in mango orchards in both Districts 134 and 145 flies/three traps, respectively. In Coimbatore District’s guava orchard and Dindigul District's mango orchard, trap catches revealed a significant positive correlation with rainfall. The subsequent weather parameters like max. temperature, min. temperature, wind speed, and relative humidity, were either positively or negatively correlated with trap catches in mango and guava orchards. In multiple regression analysis, the maximum predictability was seen in mango orchard (75.54%) located in Coimbatore District and the minimum (66.68%) in mango orchard located in Dindigul District.

IDENTIFIKASI HAMA LALAT BUAH (Diptera: Tephritidae) PADA BEBERAPA VARIETAS TANAMAN MANGGA (Mangifera indica L.) DI KABUPATEN LOMBOK UTARA

This study aims to identify fruit flies that attack several varieties of mangoes in North Lombok District. The method used in this study is descriptive with survey techniques. Sampling was done by purposive random sampling. The implementation of the activity was carried out at 17 location points of the farmers' mango orchards in the North Lombok Regency. The identification results found two genera of fruit flies that attack seven varieties of mango, namely Bactrocera and Zeugodacus. The genus Bactrocera found 12 species, namely B. pallida, B. musae, B. correcta, B. pyrifoliae, B. dorsalis, B. neohumeralis, B. tuberculata, B. carambolae, B. opiliae, B. endiandrae, B. kandiensis and B. papayae, while the genus Zeugodacus found two species, namely Z. tau and Z. depressus. Bactrocera pyrifoliae is a fruit fly species that dominates mango varieties in North Lombok Regency with a dominance level of 0.59. One species of fruit fly was found belonging to the Quarantine Plant Pest Organisms (OPTK) group A2, namely Bactrocera musae. The diversity of fruit flies is 2.86, the abundance is 95.87%, and the dominance is 0.70.

Ecotone effect on the fruit fly assemblages (Diptera: Tephritidae) in natural and anthropized environments.

Various species of fruit flies are important pests of fruit cultures and in some crop of vegetables worldwide. Studies of their population patterns, ecological processes and mechanisms that influence their sampling and distribution in the ecosystems, provides important information to support researches on species diversity and ecologically based pest control programs. The aims of this paper were to analyze the patterns of fruit fly species: diversity, abundance and composition in the margin and inside of a fragment of native forest (35 ha); on the margin and in the inside a commercial orchard (2.5 ha). This research was carried out in transects in a fragment of semideciduous forest and in a commercial orchard in the region of Dourados, Mato Grosso do Sul, Brazil. A total of 1,918 adult fruit flies: 1,350 Ceratitis capitata (Wiedemann) (♂♂+♀♀) and 568 of the genus Anastrepha Schiner (275♂♂ + 293♀♀) from six infrageneric groups and 12 different species were captured. C. capitata (80%), and Anastrepha sororcula Zucchi were the most abundant species, being this last one representing 50.68% of individuals in the genus Anastrepha. There are significant differences in species diversity of fruit flies caught in the traps installed in the edge and inside of both environments: traps installed in the forest edge had higher diversity index (H' = 2.13) in compare to the inside of forest (H' = 1.67), with the same pattern repeated in the orchard: edge (H' = 0.55) and inside (H' = 0.41). The results in this paper corroborate with the prediction that in ecotonal areas between environments there are higher diversity in compare with the inside of each of the confronting ecosystems. The technique proposed here saves time, effort and resources in rapid inventories for sampling fruit fly species richness in natural forests and large fruit tree orchards.

Spatio‐temporal co‐occurrence of Anastrepha species in papaya orchards in the state of Espírito Santo, Brazil

AbstractThe variation in co‐occurrence between pests may influence spatio‐temporal persistence strategies of species, with implications for pest management. The co‐occurrence of Anastrepha species (Diptera: Tephritidae) in papaya orchards was investigated in areas at different distances from a native forest reserve in Espírito Santo, Brazil, to investigate positive, negative, or random interactions, understood as potential coexistence, interspecific competition, or neutral interactions, respectively. Data were obtained from a papaya‐growing area divided into three sub‐areas – a buffer zone bordering the forest, neighbourhood 1, and neighbourhood 2 – and the co‐occurrence results were compared with the entire area. The buffer zone along the protected native Atlantic Forest (semideciduous seasonal forest vegetation) was created to filter negative environmental impacts. Neighbourhood 1 was closer to the forest than neighbourhood 2. Based on a long‐term dataset from 1998 to 2010, co‐occurrence of fruit flies was investigated by comparing two periods: from 1998 to 2003, when a special pest management system was implemented, termed the systems approach, and from 2004 to 2010, showing the impact of this system on fruit fly diversity. The strength of interaction between species was also evaluated for significant co‐occurrences. The patterns of co‐occurrence and interaction of Anastrepha species were influenced by area and time. In all scenarios, only positive and random associations were observed, with Anastrepha fraterculus (Wiedemann) and Anastrepha obliqua (Macquart) showing the most positive associations. Co‐occurrence was greater in areas farther from the reserve. However, the strength of the interaction between the areas showed little variation. We concluded that the distance from the reserve and the pest management (systems approach) influenced co‐occurrence, as species co‐occurred much more frequently when collected far from the native forest. The impact during the period of the systems approach (1998–2003) provided fewer positive associations, but with stronger interactions over time. These results confirm that identifying pest co‐occurrence distribution patterns in crops and the respective neighbourhoods can be helpful in improving strategies for pest management associated with a systems approach.

Species diversity and infestation indices of fruit flies (Diptera: Tephritedae) in guava ecosystem of southwest Haryana

The fruit flies are major pests of fruits and cause direct damage to guava fruits which has great impact on quality and quantity of fruits. In the present study, fruit fly diversity and infestation indices in guava fruits were investigated. Pheromone traps were installed, captured adults of species were examined and diversity was recorded. Two species viz Bactrocera dorsalis Hendel and B zonata Saunders were found in guava ecosystem in southwest Haryana. During the study, 1,179 specimens of fruit flies were collected from the traps and 179 from the fruits. The results revealed that B dorsalis was dominant species over B zonata which were 55.13 and 44.87 per cent respectively. While studying fruit fly diversity from infested fruits, 57.22 per cent of flies were those of B dorsalis and 42.22 per cent of B zonata. The mean pupal viability was found 52.49 per cent for entire observation period. The infestation indices were 45.88 pupae/kg and 7.8 pupae/fruit recorded during 2nd fortnight of September 2020.

Diversity of Fruit Flies (Diptera: Tephritidae) on Sweet Orange Plantations in Central Bangka Regency, Bangka Belitung Islands Province

Central Bangka Regency is one of central orange production in Bangka Belitung Islands Province. One of the important pests of sweet orange plants is fruit flies (Diptera: Tephritidae). This research aimed to study the diversity of fruit flies (Diptera: Tephritidae) on sweet orange plantations in Central Bangka Regency, Bangka Belitung Islands Province. The fruit flies were collected using Lynfield traps which installed in three villages producing sweet oranges in Central Bangka Regency. The types of attractants used to trap male fruit flies are methyl eugenol and cue lure. The fruit flies collected during the study were 4795 individuals, 3 genera, and 14 species. Fruit flies collected from the methyl eugenol were different compared to those collected using lure attractant trap. Most species (13 species) of fruit fly were found in Terentang village, and the rest was same (9 species). Bactrocera carambolae and B. dorsalis were the dominant fruit fly species in the three research locations.

Fruit fly species diversity, population dynamics and infestation rate during fruiting season of snake gourd

A monitoring survey was conducted in snake gourd agro ecosystem during the fruiting season at Gangnapur, West Bengal, India to generate extensive data on fruit fly diversity, population dynamics, infestation rates and evaluate the effect of trapping on fruit fly infestation rates vis-à-vis benefit cost ratio of snake gourd cultivation. Methyl Eugenol and Cue Lure traps were used to assess the fruit fly species diversity. The number of adult male flies trapped was used to calculate fruit fly per trap per day and assess the population dynamics. Bactrocera dorsalis, Zeugodacus cucurbitae and Zeugodacus tau were the predominant fruit fly species during the fruiting season. Maximum species diversity and evenness estimates were registered by Methyl Eugenol traps. Species richness estimates for Cue Lure and Methyl Eugenol traps during the entire experiment are analogous due to the trapping of two different types of fruit fly species. Zeugodacus cucurbitae is the most abundant species in the snake gourd ecosystem and dominated the community of fruit flies throughout the study. Fruit fly per trap per day values indicated that the Gangnapur area is at infestation level, for Zeugodacus cucurbitae and Bactrocera dorsalis, while, for Zeugodacus tau the area is at suppression level. The rate of infestation varied from 6.93 % to 44.09 %. Farmers with traps in their fields reported a benefit cost ratio of 0.73 to 4.63, while the no-trap group reported 0.34 to 1.56. This study at Gangnapur has proven the beneficial effect of trapping on the benefit cost ratio of snake gourd cultivation.

Diversity and species composition of fruit flies (Diptera: Tephritidae) in Lombok Island, Indonesia

Abstract. Hudiwaku S, Himawan T, Rizali A. 2021. Diversity and species composition of fruit flies (Diptera: Tephritidae) in Lombok Island, Indonesia. Biodiversitas 22: 4608-4616. Fruit flies (Diptera: Tephritidae) are pests of several horticultural crops that can reduce the quality and quantity of fruit production. Information on fruit flies in Lombok Island, Indonesia, is still limited. However, it is predicted to have a high diversity of fruit flies because this island belongs to the Wallacea region. The aims of this research was to study the diversity and species composition of fruit flies in different habitat types in Lombok Island. The research was carried out on two habitat types, i.e., tropical rainforest and orchard with each habitat type consisted of three different sites that located spread across Lombok Island as replication. The research was carried out on two habitat types, i.e., tropical rainforest and orchard, with each habitat type consisted of three different sites spread across Lombok Island as replication. A sampling of fruit flies was conducted using parapheromone traps from March to June 2020. Twenty-two species and 210,267 individual fruit flies were collected from all locations during the study period. The most dominant species were Bactrocera carambolae, Bactrocera limbifera, Zeugodacus caudatus, and Bactrocera dorsalis. Based on the ANOVA, habitat types significantly affected the abundance but not the species richness of fruit flies. The visualization results obtained from the NMDS ordination indicated a difference in the species composition of fruit flies between the two habitats. In conclusion, habitat types are an essential factor in shaping the community of fruit flies in Lombok Island.

DIVERSITY OF FRUIT FLIES (DIPTERA: TEPHRITIDAE) ATTRACTED BY ME LURE IN CSC-BG GERMPLASM CARAMBOLA PLANTATION

Diversity of fruit flies (Diptera: tephritidae) attracted by me lure in CSG-BG germplasm carambolae plantation. Germplasm Garden (KPN) Cibinong Science Center-Botanical Garden (CSC-BG) is one of the gardens that conserve and utilizes Indonesian local germplasm, one of which is carambolae. The current problem in KPN is the attack of fruit flies (Bactrocera spp.). This study was conducted to determine the diversity of fruit flies in the KPN carambola plantation area. A total of eight traps were placed randomly in the plantation area. Each trap contained 1 mL of methyl eugenol pheromone solution. Identification of fruit flies was carried out based on three morphological differences, they were wings, abdomen, and hind legs. There were 317 fruit flies collected and separated into three species, namely Bactrocera carambolae, B. papayae, dan B. umbrosa, of which B. carambolae had a dominance index of 0.74 and abundance index of 85%. Data on the types of fruit flies can be used as primary data to determine the preparation of pest prevention efforts.

Long-term fruit fly monitoring and impact of the systems approach on richness and abundance

AbstractWe investigated ecological patterns of richness and abundance of Anastrepha fruit flies, based on a long-term series with a dataset from 1998 to 2010, subdivided into four subseries describing pest management through the systems approach (integration of different measures, at least two of which act independently, with cumulative effects), and its impact on abundance and diversity of fruit flies. Richness and abundance were influenced by time and distance but to different extents. Spatio-temporal analysis taking into account the implementation of the systems approach revealed clear effects of the pest management on fruit fly richness and abundance. However, abundance was affected by the systems approach three years before richness was. Abundance and richness also showed different relationships with time and distance between orchards and forest. The Gompertz model, used to describe the relationship between area and species richness, was the function that showed the best fit to the data. The richness-partitioning analysis, which decomposes beta diversity, indicated different distributions of richness values and predictions for additive partitioning that were directly associated with the implementation of the systems approach. The spectral analysis projected different trends for peaks, indicating that the systems approach is able to delay the time for new population peaks of fruit flies.

The abundance and diversity of fruit flies and their parasitoids change with elevation in guava orchards in a tropical Andean forest of Peru, independent of seasonality.

Lower elevations are generally thought to contain a greater abundance and diversity of insect communities and their natural enemies than higher elevations. It is less clear, however, how changes in seasons influence this pattern. We conducted a 2-year study (2013‒2014) in guava orchards located in a tropical Andean forest of Peru to investigate differences in fruit flies (Diptera: Tephritidae) and their parasitoid communities at two elevations and over two seasons. Fruit fly traps were installed, monitored, and guava fruits were sampled from eight orchards at low (800–950 m above sea level) and high (1,700–1,900 m above sea level) elevations and during the dry and rainy seasons. At each orchard, adult fruit fly trap captures and emergence of fruit flies and their parasitoids from guava fruit were quantified to determine their abundance and species composition. There was a greater abundance and species richness of fruit flies captured in traps at lower elevations, as well as higher abundance and species evenness of fruit flies that emerged from fruit, indicating that lower elevations are associated with larger fruit fly populations. The abundance, species richness and diversity of parasitoids were also greater at lower elevations. Consequently, guava fruit infestation and fruit fly parasitism rates were also greater at lower elevations. Seasonality also influenced fruit fly populations with a greater number of flies emerging from guava fruit and more fruit infested in the rainy season. However, seasonality had no effect on parasitoid population parameters or rate of parasitism, nor did it interact with elevation as an influence of populations of fruit flies or their parasitoids in guava orchards. This study highlights the importance of examining both elevation and seasonality for a better understanding of the population dynamics of fruit flies and their parasitoids in tropical agroecosystems.