Diverse demands have promoted the rapid development of the cold chain logistics industry. In the paper, a novel approach for calculating the comprehensive carbon emission cost was proposed and the front warehouse mode was analyzed under the background of energy conservation and emission reduction. To solve the two-echelon low-carbon location-routing problem (2E-LCLRP), a mathematical model considering operating cost, total transportation cost, fixed cost, refrigeration cost, cargo damage cost, and comprehensive carbon emission cost was proposed to determine the minimum total cost. A hybrid ant colony optimization (HACO) algorithm based on an elbow rule and an improved ant colony optimization (IACO) algorithm was proposed to solve the 2E-LCLRP. According to the elbow rule, the optimal number of front warehouses was determined and an IACO algorithm was then designed to optimize vehicle routes. An adaptive hybrid selection strategy and an optimized pheromone update mechanism were integrated into the HACO algorithm to accelerate convergence and obtain global optimal solutions. The proposed model and algorithm were verified through the case study of the 2E-LCLRP in Nanjing, China. The HACO algorithm outperformed the original ant colony optimization (ACO) algorithm in terms of convergence rate and solution quality. This study provides significant insights for enhancing heuristic algorithms as well as valuable research methods. Furthermore, the results can help cold chain logistics companies in balancing economic costs and environmental benefits and address cold chain distribution of agricultural products.
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