Diversity Order Research Articles

Extraction recovery and speciation of selenium in Se-enriched yeast.

The complete characterization of selenium-enriched yeast in terms of selenium species has been the goal of extensive research for the last three decades. This contribution addresses the two outstanding questions: the mass balance of the identified and reported selenium species and the possible presence of inorganic selenium. For this purpose, four procedures have been designed combining, in diverse order, the principal steps of selenium speciation analysis in Se-rich yeast: extraction of the Se-metabolome, derivatization of cysteine and Se-cysteine (SeCys) residues, proteolysis, and definitive Se recovery using SDS extraction, followed by mineralization. The recovery of selenium in each step and its speciation were controlled by ICP MS and by reversed-phase HPLC-ICP MS, respectively. The study, carried out for the SELM-1 reference material, demonstrated the presence of about 10% of inorganic selenium and a serious risk of losses of SeCys during derivatization and proteolysis. As result of our work, we postulate the following values for SELM-1: Se-metabolome fraction (SeMF) 14.8 ± 0.7%; total selenomethionine (SeMet) 66.2 ± 2.7% (including ca. 1.5% SeMet present in the SeMF); total SeCys 12.5 ± 1.5% (including 2% of SeCys present in the Se-MF); total inorganic selenium 9.7 ± 1.7%, accounting for > 99.8% of the selenium.

First record of the lobster cockroach Nauphoeta cinerea (Olivier, 1789) (Insecta: Blattodea) in Europe with remarks on synanthropic cockroaches of the Iberian Peninsula

AbstractGlobalization has facilitated the introduction and establishment of organisms beyond their natural ranges, leading to significant ecological and economic consequences. While many non‐native species are well documented, others remain understudied, either due to their unknown impact or morphological similarities with native species. This study addresses such a case, focusing on synanthropic cockroaches within the order Blattodea. Despite the order's diversity, less than 1% of known species are recorded in urban environments. While certain species like Periplaneta americana, Blatta orientalis and Blattella germanica are well distributed and studied, others remain largely unknown. This research unveils the first established population of Nauphoeta cinerea in Europe, specifically in Spain (Asturias, northern Iberian Peninsula), with specific genetic and morphological confirmation. This study also explores potential pathways of introduction for N. cinerea. Long‐distance transport by ships or escape/release from captivity are proposed as potential pathways for the newly established populations in Asturias. The species' high fecundity and parthenogenetic reproduction raise concerns about its potential invasiveness. Additionally, this study includes unpublished data on various synanthropic cockroaches in Asturias (with the first records of P. lateralis and Supella longipalpa for this area), drawn from specimens deposited in biological collections and some extra collections. An identification key for all Spanish non‐native synanthropic cockroach species is provided to assist in future detections. This research emphasizes the imperative need for robust monitoring systems, highlights the scarcity of scientific information regarding synanthropic cockroach distribution and underscores the pivotal role of biological collections in advancing our understanding of invasive species.

A Journey Towards a Non‐Western International Relations Theory: Non‐Western Conceptualization of the English School

The purpose of this study is to examine non‐Western international relations theory (IRT) and explore, at the theoretical level, the potential for epistemological autonomy from the confines of Western‐centered epistemology prevalent in existing IRT. As a consequence, the non‐Western world, subjected to colonialist expansion and modernization and often regarded as merely the leaves, has been relegated to a passive role within the international system, becoming a disengaged object of little interest. However, what has existed cannot be entirely erased. While it is undeniable that international politics was predominantly developed based on a Western‐centric epistemology, other ancient civilizations had diverse international systems and orders that molded their identities as regional international societies. Consequently, the study provides a crucial backdrop for theoretical endeavors aimed at integrating the voices and experiences of the Global South into IRT, which has traditionally been dominated by Western perspectives. This study seeks to explore the potential for transcending Western intellectual hegemony by critiquing the limitations of a singular worldview and expanding and transforming the English School Theory (EST). It seeks to investigate the possibility of moving beyond Western‐centricity in international relations theory by conducting a historical comparison of civilizations across countries and the associated disciplines within civilizations as a field.

Evolutionary Patterns of Maternal Recognition of Pregnancy and Implantation in Eutherian Mammals.

The implantation of the embryo into the maternal endometrium is a complex process associated with the evolution of viviparity and placentation in mammals. In this review, we provide an overview of maternal recognition of pregnancy signals and implantation modes in eutherians, focusing on their diverse mechanisms and evolutionary patterns. Different pregnancy recognition signals and implantation modes have evolved in eutherian mammals, reflecting the remarkable diversity of specializations in mammals following the evolution of viviparity. Superficial implantation is the ancestral implantation mode in Eutheria and its major clades. The other modes, secondary, partially, and primary interstitial implantation have each independently evolved multiple times in the evolutionary history of eutherians. Although significant progress has been made in understanding pregnancy recognition signals and implantation modes, there is still much to uncover. Rodents and chiropterans (especially Phyllostomidae) offer valuable opportunities for studying the transitions among implantation modes, but data is still scarce for these diverse orders. Further research should focus on unstudied taxa so we can establish robust patterns of evolutionary changes in pregnancy recognition signaling and implantation modes.

IFIT1 is rapidly evolving and exhibits disparate antiviral activities across 11 mammalian orders.

Mammalian mRNAs possess an N7-methylguanosine (m7G) cap and 2'O methylation of the initiating nucleotide at their 5' end, whereas certain viral RNAs lack these characteristic features. The human antiviral restriction factor IFIT1 recognizes and binds to specific viral RNAs that lack the 5' features of host mRNAs, resulting in targeted suppression of viral RNA translation. This interaction imposes significant host-driven evolutionary pressures on viruses, and many viruses have evolved mechanisms to evade the antiviral action of human IFIT1. However, little is known about the virus-driven pressures that may have shaped the antiviral activity of IFIT1 genes across mammals. Here, we take an evolution-guided approach to show that the IFIT1 gene is rapidly evolving in multiple mammalian clades, with positive selection acting upon several residues in distinct regions of the protein. In functional assays with 39 IFIT1s spanning diverse mammals, we demonstrate that IFIT1 exhibits a range of antiviral phenotypes, with many orthologs lacking antiviral activity against viruses that are strongly suppressed by other IFIT1s. We further show that IFIT1s from human and a bat, the black flying fox, inhibit Venezuelan equine encephalitis virus (VEEV) and strongly bind to Cap0 RNAs. Unexpectedly, chimpanzee IFIT1, which differs from human IFIT1 by only 8 amino acids, does not inhibit VEEV infection and exhibits minimal Cap0 RNA-binding. In mutagenesis studies, we determine that amino acids 364 and 366, with the latter undergoing positive selection, are sufficient to confer the differential anti-VEEV activity between human and chimpanzee IFIT1. These data suggest that virus-host genetic conflicts have influenced the antiviral specificity of IFIT1 across diverse mammalian orders.

Medium and large-sized mammals in human-dominated landscape of the former Dhidhessa wildlife sanctuary, Western Ethiopia

Abstract Assessment of medium- and large-sized mammals’ species diversity, abundance, distribution, and habitat association is necessary to promote their conservation measures. The study was conducted in the former Dhidhessa wildlife sanctuary (FDWS), to assess the medium- and large-sized mammals’ composition, distribution, and diversity. Transect lines were used to collect the data. A total of 1,146 ± 91 individuals belonging to 25 species, seven orders, and 14 families were recorded. Among these, 607 ± 186.5 (52.97%) were recorded during the wet season, whereas 539 ± 144.5 (47.03%) during the dry season. The order Carnivora was the most diverse one, followed by Artiodactyla and Primates, respectively, while Rodentia, Hyracoidea, Tubulidentata, and Lagomorpha were the least diverse orders. Olive baboon (Papio anubis) was the most abundant species (19.2%), followed by Hippopotamus (Hippopotamus amphibious) (17.63%) and Vervet monkey (Chlorocebus aethiops) (13.18%). Species diversity was higher in the savanna-wooded than in the riverine forest during both the wet (H’ = 2.72, Fisher’s alpha = 6.33) and dry (H’= 2.79, Fisher’s alpha = 6.26) seasons. Species evenness was higher in the savanna-wooded habitat than in the riverine forest habitat during both the wet (J’ = 0.87 and J’ = 0.77, respectively) and dry (J’ = 0.9 and J’ = 0.80, respectively) seasons. The highest similarity in species composition was between the wet and dry seasons in the riverine forest habitat (SI = 85.58%). The lowest similarity was recorded between the dry and wet seasons of the riverine forest and the wet season of the savanna wooded habitat (SI = 45.46% and SI = 46.49%, respectively). The study suggests that mammals are at risk of anthropogenic factors in the area. So, an urgent conservation measure that involves the participation of the local community has paramount significance for the sustainable conservation of mammals in the former Dhidhessa wildlife sanctuary.

Some New Estimations of Ostrowski-Type Inequalities for Harmonic Fuzzy Number Convexity via Gamma, Beta and Hypergeometric Functions

This paper demonstrates several of Ostrowski-type inequalities for fuzzy number functions and investigates their connections with other inequalities. Specifically, employing the Aumann integral and the Kulisch–Miranker order, as well as the inclusion order on the space of real and compact intervals, we establish various Ostrowski-type inequalities for fuzzy-valued mappings (F·V·Ms). Furthermore, by employing diverse orders, we establish connections with the classical versions of Ostrowski-type inequalities. Additionally, we explore new ideas and results rooted in submodular measures, accompanied by examples and applications to illustrate our findings. Moreover, by using special functions, we have provided some applications of Ostrowski-type inequalities.

RIBAP: a comprehensive bacterial core genome annotation pipeline for pangenome calculation beyond the species level

Microbial pangenome analysis identifies present or absent genes in prokaryotic genomes. However, current tools are limited when analyzing species with higher sequence diversity or higher taxonomic orders such as genera or families. The Roary ILP Bacterial core Annotation Pipeline (RIBAP) uses an integer linear programming approach to refine gene clusters predicted by Roary for identifying core genes. RIBAP successfully handles the complexity and diversity of Chlamydia, Klebsiella, Brucella, and Enterococcus genomes, outperforming other established and recent pangenome tools for identifying all-encompassing core genes at the genus level. RIBAP is a freely available Nextflow pipeline at github.com/hoelzer-lab/ribap and zenodo.org/doi/10.5281/zenodo.10890871.

MEDICAL PLURALISM IN BRITISH PUNJAB: NECESSITY OF COEXISTENCE, CONVERGENCE IN DIVERSE SOCIO-CULTURAL FABRIC

This research paper explores the coexistence and convergence of plural healthcare practices in British Punjab with a prime focus on the need for a diverse social order to accommodate multiple healthcare setups. The interdisciplinary conceptual framework of this research rests on the debate around the dynamics of medical pluralism and its implications for healthcare delivery, while combining medical anthropology, and historical sociology. to understand. The objective is to explore how different medical systems, both indigenous and contemporary, influence and interact with each other, hence serving as the driving forces towards their coexistence and sometimes convergence of these systems. The findings reveal a complex and dynamic landscape of medical pluralism in the then British Punjab, with traditional Ayurvedic, Unani, and homoeopathic practices showcasing interactions between traditional and Western medical practices. The findings reveal that recognizing and integrating various medical traditions and practices is crucial for ensuring accessible and effective healthcare services for the population.

Rabies virus circulation in a highly diverse bat assemblage from a high-risk area for zoonoses outbreaks in the Brazilian Amazon

Bats are the second most diverse order of mammals and play a central role in ecosystem dynamics. They are also important reservoirs of potentially zoonotic microorganisms, of which rabies virus is the most lethal among the bat-transmitted zoonotic pathogens. Importantly, recent outbreaks of human rabies have been reported from the Brazilian Amazon. Here we present a survey of bat species and rabies virus (RABV) circulation in a bat assemblage in the Marajó region, northern Brazil. Using data from mist-net captures and bioacoustic sampling, 56 bat species were recorded along the Jacundá River basin over a 10-day expedition in November 2022. For the investigation of RABV, we used the direct fluorescent antibody test (DFAT) and the rapid fluorescent focus inhibition test (RFFIT). In total, 159 bat individuals from 22 species were investigated for RABV. Five adults of the common vampire bat, Desmodus rotundus, showed RABV-specific antibodies in serum samples. Additionally, we report on local residents with injuries caused by D. rotundus bites and the occurrence of colonies of non-hematophagous bats from different species roosting inside human residences. This scenario raises concerns about the risks of new cases of human rabies and other zoonotic diseases associated with bats in the region and highlights the need for epidemiological surveillance and mitigation measures to prevent outbreaks of emerging infectious diseases.

Performance analysis of fixed gain relay-assisted vertical UWOC systems with generalized misalignment fading model

Underwater wireless optical communication (UWOC) as a potential underwater transmission technology has attracted increasing attention due to its high speed, low delay, and wide range of applications. In addition to absorption and scattering, ocean turbulence fading and misalignment fading seriously affect system performance. Thus, the relay assistance methods have been presented as an effective technique for fading mitigation in optical channels. In this paper, we propose a fixed gain amplify-and-forward (AF) relay-assisted vertical UWOC system with a generalized misalignment fading model over multi-layer cascaded gamma-gamma turbulence channels. For the relay-assisted system, the cumulative distribution function (CDF) and the probability density function (PDF) of the end-to-end instantaneous signal-to-noise ratio (SNR) are derived with the help of the Meijer-G function and the bivariate Fox-H function. Subsequently, based on these statistical analyses of SNR, we derive the closed-form expressions of the outage probability and ergodic capacity. Furthermore, we provide a very tight asymptotic expression for the outage probability in terms of simple functions under high SNR, and the diversity order is analyzed. Finally, results from Monte Carlo simulations are used to validate our derived results. Numerical results for different relay position deployments and alignment error levels reveal that the relay-assisted vertical UWOC system performs better when the relay node is located near the source, while the increase of alignment error level can damage the system performance.

The Wasps (Hymenoptera) from Lower Cretaceous Lebanese and Spanish Ambers

Hymenoptera is the fourth-most diverse insect order today, including wasps, bees, bumblebees, and ants. They show a wide panoply of modes of life, such as herbivory, predation, parasitoidism, pollination, and eusociality. This group also includes a great number of extinct species from both amber and compression outcrops. Hymenopterans probably originated in the Paleozoic, although their oldest record is from the Middle or Late Triassic, and their diversity expanded since the Cretaceous. Here, we present a review of the Hymenoptera in Lower Cretaceous ambers from Lebanon (Barremian) and Spain (Albian), which is pivotal for the study of hymenopteran evolution. Hymenoptera in Lebanese ambers are represented by 32 species in 22 genera within 15 families, while in Spanish ambers, they correspond to 49 species in 40 genera within 18 families. Most of these species belong to the ‘Parasitica’, and only a few species have been assigned to the Aculeata. The group ‘Symphyta’ is represented by one species in Spanish amber. The paleobiogeography and possible paleobiologies of the species in these ambers are reviewed. Furthermore, checklists for all Hymenoptera species in Lebanese and Spanish ambers are provided.

Secrecy Outage Probability and Secrecy Diversity Order of Alamouti STBC with Decision Feedback Detection over Time-Selective Fading Channels

Secrecy Outage Probability and Secrecy Diversity Order of Alamouti STBC with Decision Feedback Detection over Time-Selective Fading Channels

The diverse liver viromes of Australian geckos and skinks are dominated by hepaciviruses and picornaviruses and reflect host taxonomy and habitat

Abstract Lizards have diverse ecologies and evolutionary histories, and represent a promising group to explore how hosts shape virome structure and virus evolution. Yet, little is known about the viromes of these animals. In Australia, squamates (lizards and snakes) comprise the most diverse order of vertebrates, and Australia hosts the highest diversity of lizards globally, with the greatest breadth of habitat use. We used meta-transcriptomic sequencing to determine the virome of nine co-distributed, tropical lizard species from three taxonomic families in Australia and analyzed these data to identify host traits associated with viral abundance and diversity. We show that lizards carry a large diversity of viruses, identifying more than thirty novel, highly divergent vertebrate-associated viruses. These viruses were from nine viral families, including several that contain well known pathogens, such as the Flaviviridae, Picornaviridae, Bornaviridae, Iridoviridae, and Rhabdoviridae. Members of the Flaviviridae were particularly abundant across species sampled here, largely belonging to the genus Hepacivirus: fourteen novel hepaciviruses were identified, broadening the known diversity of this group and better defining its evolution by uncovering new reptilian clades. The evolutionary histories of the viruses studied here frequently aligned with the biogeographic and phylogenetic histories of the hosts, indicating that exogenous viruses may help infer host evolutionary history if sampling is strategic and sampling density high enough. Notably, analysis of alpha and beta diversity revealed that virome composition and richness in the animals sampled here was shaped by host taxonomy and habitat. In sum, we identified a diverse range of reptile viruses that broadly contributes to our understanding of virus-host ecology and evolution.

Effects of Film Mulching on Soil Microbial Diversity and Community Structure in the Maize Root Zone under Drip Irrigation in Northwest China

Mulching is a widely used agricultural water conservation measure in the semiarid regions of Northwest China. In order to explore the response process of different film mulching methods to soil microorganisms, we characterized the effect of different film mulching methods on soil microbial diversity and community structure characteristics in the root zone of drip-irrigated maize during the heading and maturity stages using high-throughput sequencing of 16SrDNA and ITS amplicons combined with bioinformatics analysis. Full mulching (FM) was contrasted to controls of no mulching (NM) and half-mulching (HM), yielding an order of microbial diversity, abundance, and evenness scores of HM > FM > NM. The HM and FM treatments reduced the relative abundance of Proteobacteria and Actinobacteria (the most abundant bacteria) in the bacterial community structure but increased that of Acidobacteria and Chloroflexi. In the fungal community structure, HM decreased the abundance of Sordariomycetes but increased that of Eurotiomycetes (the most abundant fungi). The abundance and community structure of bacteria were significantly correlated with soil temperature and those of fungi with pH. HM improved network complexity and competitive relationships among bacteria, while FM increased the relationship between fungal groups and the symbiosis of fungal communities. HM significantly increased maize yield (20.37% and 6.01% above NM and FM, respectively). In summary, full mulching was more favorable than no mulching for soil microbial diversity and community structure composition, but soil microbial diversity and yield responded better to half-mulching. These results provide a background for improving the yield of drip-irrigated maize and protecting the microbial ecosystems of farmland soils.

Performance Prediction of Power Beacon-Aided Wireless Sensor-Powered Non-Orthogonal Multiple-Access Internet-of-Things Networks under Imperfect Channel State Information

In this paper, we investigate a novel power beacon (PB)-aided wireless sensor-powered non-orthogonal multiple-access (NOMA) Internet-of-Things (IoT) network under imperfect channel state information (CSI). Furthermore, the exact expression outage probability (OP) of two IoT users is derived to analyze the performance of the considered network. To give further insight, the expression asymptotic OP and diversity order are also expressed when the transmit power at the PB goes to infinity. Furthermore, a deep neural network (DNN) framework is proposed to concurrently forecast IoT users’ OP in relation to real-time setups for IoT users. Additionally, when compared to the traditional analysis, our created DNN shows the shortest run-time prediction, and the outcomes predicted by the DNN model almost match those of the simulation. In addition, numerical results validate our analysis, simulation, and prediction through a Monte Carlo Simulation. Furthermore, the results show the impact of the main parameter on our proposed system. Finally, these findings show that NOMA performs better than the conventional orthogonal multiple-access (OMA) techniques.

Optimal Decoding Order and Power Allocation for Sum Throughput Maximization in Downlink NOMA Systems.

In this paper, we consider a downlink non-orthogonal multiple access (NOMA) system over Nakagami-m channels. The single-antenna base station serves two single-antenna NOMA users based on statistical channel state information (CSI). We derive the closed-form expression of the exact outage probability under a given decoding order, and we also deduce the asymptotic outage probability and diversity order in a high-SNR regime. Then, we analyze all the possible power allocation ranges and theoretically prove the optimal power allocation range under the corresponding decoding order. The demarcation points of the optimal power allocation ranges are affected by target data rates and total power, without an effect from the CSI. In particular, the values of the demarcation points are proportional to the total power. Furthermore, we formulate a joint decoding order and power allocation optimization problem to maximize the sum throughput, which is solved by efficiently searching in our obtained optimal power allocation ranges. Finally, Monte Carlo simulations are conducted to confirm the accuracy of our derived exact outage probability. Numerical results show the accuracy of our deduced demarcation points of the optimal power allocation ranges. And the optimal decoding order is not constant at different total transmit power levels.

An atlas of spider development at single-cell resolution provides new insights into arthropod embryogenesis

Spiders are a diverse order of chelicerates that diverged from other arthropods over 500 million years ago. Research on spider embryogenesis, particularly studies using the common house spider Parasteatoda tepidariorum, has made important contributions to understanding the evolution of animal development, including axis formation, segmentation, and patterning. However, we lack knowledge about the cells that build spider embryos, their gene expression profiles and fate. Single-cell transcriptomic analyses have been revolutionary in describing these complex landscapes of cellular genetics in a range of animals. Therefore, we carried out single-cell RNA sequencing of P. tepidariorum embryos at stages 7, 8 and 9, which encompass the establishment and patterning of the body plan, and initial differentiation of many tissues and organs. We identified 20 cell clusters, from 18.5 k cells, which were marked by many developmental toolkit genes, as well as a plethora of genes not previously investigated. We found differences in the cell cycle transcriptional signatures, suggestive of different proliferation dynamics, which related to distinctions between endodermal and some mesodermal clusters, compared with ectodermal clusters. We identified many Hox genes as markers of cell clusters, and Hox gene ohnologs were often present in different clusters. This provided additional evidence of sub- and/or neo-functionalisation of these important developmental genes after the whole genome duplication in an arachnopulmonate ancestor (spiders, scorpions, and related orders). We also examined the spatial expression of marker genes for each cluster to generate a comprehensive cell atlas of these embryonic stages. This revealed new insights into the cellular basis and genetic regulation of head patterning, hematopoiesis, limb development, gut development, and posterior segmentation. This atlas will serve as a platform for future analysis of spider cell specification and fate, and studying the evolution of these processes among animals at cellular resolution.

Hybrid selection combining scheme for correlated cooperative communication over [formula omitted]-[formula omitted] fading channels

Due to poor (limited) scattering, a correlation among channels is inevitable, resulting in performance degradation of wireless communication systems. In this paper, we propose a hybrid selection combining (SC), also known as switched scaled – SC for correlated cooperative communication over κ-μ fading channel. By using paired error analysis, we derive the end-to-end symbol error probability of this system over bivariate κ-μ channel fading with M-ary phase shift keying (MPSK) modulation. In addition, we obtain significant parameters of hybrid SS-SC, such as the optimum scaling factor and optimum threshold by using the second derivative test. For every signal to noise ratio (SNR), we obtain different optimum thresholds and scaling factors. Our analytical results were found to overlap significantly with those of the simulation results. They were also compared with the variants of SC and switched diversity techniques. Our study demonstrates that the proposed hybrid technique performs better than the other SC and switched diversity schemes over correlated and uncorrelated fading channels. Furthermore, we derive an asymptotic symbol error probability for the proposed system, and the asymptotic results are in agreement with the analytical results at higher SNRs. Our study also shows that the diversity order of the system depends on the mutually exclusive events of the proposed technique.

Kübreviyye Tarîkati Özelinde Tasavvufta Zikr

Among various orders of Islamic Sufism, , one of the common rituals is dhikr. While sharīa places significant emphasis on the repetition of different dhikr formulas, it does not mandate its practice. However, within the diverse orders of Sufism, dhikr is viewed as a fundamental aspect of conduct and a cornerstone of practical mysticism. In Islamic Sufism, disciples deem it obligatory to purify the nafs. Consequently, dhikr is regarded as a means to this end across various schools and branches of Islamic Sufism. Each order of Islamic Sufism has developed its own unique instructions and rituals for its followers. The Kubrawiyya order, one of the oldest schools of Islamic Sufism, has also established its own rituals and instructions pertaining to dhikr for its disciples. The primary objective of this paper is to elucidate the essence and the quality of dhikr in the Kubrawiyya order. This includes its importance, principles, objectives, methods, and results. The analysis is based on the materials presented in the texts of the Kubrawiyya order, supplemented by references to other sources of Islamic Sufism. This scholarly approach ensures a comprehensive understanding of the role and significance of dhikr within the Kubrawiyya order specifically, and Islamic Sufism more broadly.