Diverse Lifestyles Research Articles

Transposon sequencing analysis of Bradyrhizobium diazoefficiens 110spc4

Bradyrhizobium diazoefficiens USDA110 is one of the most effective nitrogen-fixing symbionts of soybeans. Here we carried out a large-scale transposon insertion sequencing (Tn-seq) analysis of strain Bd110spc4, which is derived from USDA110, with the goal of increasing available resources for identifying genes crucial for the survival of this plant symbiont under diverse conditions. We prepared two transposon (Tn) insertion libraries of Bd110spc4 with 155,042 unique Tn insertions when the libraries were combined, which is an average of one insertion every 58.7 bp of the reference USDA110 genome. Application of bioinformatic filtering steps to remove genes too small to be expected to have Tn insertions, resulted in a list of genes that were classified as putatively essential. Comparison of this gene set with genes putatively essential for the growth of the closely related alpha-proteobacterium, Rhodopseudomonas palustris, revealed a small set of five genes that may be collectively essential for closely related members of the family Bradyrhizobiaceae. This group includes bacteria with diverse lifestyles ranging from plant symbionts to animal-associated species to free-living species.

Residential Segregation and Living Conditions. An Analysis of Social Inequalities in Catalonia from Four Spatial Perspectives

Spatial inequalities in living conditions have traditionally been attributed to geographical location, the opposition between urban and rural settings or the size of settlements. Accordingly, the geographical literature has used these oppositions to explain not only differences in access to education, work and services but also diversity of lifestyles, beliefs and even political attitudes. In recent decades, however, urban areas have extended their scope, urbanization has become more dispersed, territories have become more interdependent and spatial hierarchies have tended to weaken. At the same time, social inequalities have become more marked, as manifested spatially by residential segregation. This article puts forward the thesis that residential segregation constitutes a considerably better explanatory factor currently for the elucidation of social inequalities and differences in living conditions in regional spaces than geographical location, the urban/rural divide or the size of settlements. A set of key indicators in the population of residents in Catalonia (level of education, socio-economic position, risk of poverty, self-perceived health and life satisfaction) are therefore analyzed from various spatial perspectives to explore this argument and evaluate each indicator’s explanatory potential. The main results seem to confirm the hypothesis that the most striking spatial inequalities are associated with residential segregation.

Comparative genomics reveals a core gene toolbox for lifestyle transitions in Hypocreales fungi.

SummaryFungi have evolved diverse lifestyles and adopted pivotal new roles in both natural ecosystems and human environments. However, the molecular mechanisms underlying their adaptation to new lifestyles are obscure. Here, we hypothesize that genes shared across all species with the same lifestyle, but absent in genera with alternative lifestyles, are crucial to that lifestyle. By analysing dozens of species within four genera in a fungal order, with each genus following a different lifestyle, we find that genus‐specific genes are typically few in number. Notably, not all genus‐specific genes appear to derive from de novo birth, with most instead reflecting recurrent loss across the fungi. Importantly, however, a subset of these genus‐specific genes are shared by fungi with the same lifestyle in quite different evolutionary orders, thus supporting the view that some genus‐specific genes are necessary for specific lifestyles. These lifestyle‐specific genes are enriched for key functional classes and often exhibit specialized expression patterns. Genus‐specific selection also contributes to lifestyle transitions, and is especially associated with intensity of pathogenesis. Our study, therefore, suggests that fungal adaptation to new lifestyles often requires just a small number of core genes, with gene turnover and positive selection playing complementary roles.

Climbing Adaptations of an Enigmatic Early Arctoid Carnivoran: the Functional Anatomy of the Forelimb of Amphicynodon leptorhynchus From the Lower Oligocene of the Quercy Phosphorites (France)

This paper provides a detailed description and the functional anatomy of the forelimb of an early Oligocene genet-sized arctoid carnivoran, Amphicynodon leptorhynchus, to infer its probable lifestyle. This work represents the first insights into the functional anatomy of the postcranial skeleton and palaeoecology of an emblematic member of the first radiation of arctoid carnivorans, the poorly known Amphicynodontidae, a group very likely related to bears. So far, the Amphicynodontidae have only been studied on the basis of their cranial and dental remains. The fossil site of Itardies (MP23, lower Oligocene), in the Quercy Phosphorites (France), have yielded hundreds of postcranial remains attributed to Am. leptorhynchus, allowing a complete analysis of its locomotor behaviour in comparison to a large sample of extant carnivorans showing diverse lifestyles. The anatomy of the forelimb of Am. leptorhynchus indicates well-developed climbing skills and grasping abilities. Thus, this early arctoid was probably living mostly in trees, moving on the ground basically for foraging as observed in small modern climbing carnivorans such as the red panda or the kinkajou, although some other climbing carnivorans can also live in rocky areas.

Intravacuolar Pathogens Hijack Host Extracellular Vesicle Biogenesis to Secrete Virulence Factors.

Extracellular vesicles (EVs) have garnered significant interest in recent years due to their contributions to cell-to-cell communication and disease processes. EVs are composed of a complex profile of bioactive molecules, which include lipids, nucleic acids, metabolites, and proteins. Although the biogenesis of EVs released by cells under various normal and abnormal conditions has been well-studied, there is incomplete knowledge about how infection influences EV biogenesis. EVs from infected cells contain specific molecules of both host and pathogen origin that may contribute to pathogenesis and the elicitation of the host immune response. Intracellular pathogens exhibit diverse lifestyles that undoubtedly dictate the mechanisms by which their molecules enter the cell’s exosome biogenesis schemes. We will discuss the current understanding of the mechanisms used during infection to traffic molecules from their vacuolar niche to host EVs by selected intravacuolar pathogens. We initially review general exosome biogenesis schemes and then discuss what is known about EV biogenesis in Mycobacterium, Plasmodium, Toxoplasma, and Leishmania infections, which are pathogens that reside within membrane delimited compartments in phagocytes at some time in their life cycle within mammalian hosts. The review includes discussion of the need for further studies into the biogenesis of EVs to better understand the contributions of these vesicles to host-pathogen interactions, and to uncover potential therapeutic targets to control these pathogens.

The Crustacean Antennule: A Complex Organ Adapted for Lifelong Function in Diverse Environments and Lifestyles.

AbstractThe crustacean first antenna, or antennule, has been an experimental model for studying sensory biology for over 150 years. Investigations have led to a clearer understanding of the functional organization of the antennule as an olfactory organ but also to a realization that the antennule is much more than that. Across the Crustacea, the antennules take on many forms and functions. As an example, the antennule of reptantian decapods has many types of sensilla, each with distinct structure and function and with hundreds of thousands of chemosensory neurons expressing hundreds of genes that code for diverse classes of receptor proteins. Together, these antennular sensilla represent multiple chemosensory pathways, each with its own central connections and functions. The antennule also has a diversity of sensors of mechanical stimuli, including vibrations, touch, water flow, and the animal's own movements. The antennule likely also detects other environmental cues, such as temperature, oxygen, pH, salinity, and noxious stimuli. Furthermore, the antennule is a motor organ-it is flicked to temporally and spatially sample the animal's chemo-mechanical surroundings-and this information is used in resolving the structure of chemical plumes and locating the odor source. The antennule is also adapted to maintain lifelong function in a changing environment. For example, it has specific secretory glands, grooming structures, and behaviors to stay clean and functional. Antennular sensilla and the annuli on which they reside are also added and replaced, leading to a complete turnover of the antennule over several molts. Thus, the antennule is a complex and dynamic sensory-motor integrator that is intricately engaged in most aspects of the lives of crustaceans.

The diversity of stomatal development regulation in Callitriche is related to the intrageneric diversity in lifestyles

Significance Plant stomata are produced through divisions and differentiation of stem cells, termed meristemoids. During stomatal development, we see diverse patterns of meristemoid behavior among land plant lineages. However, both the ecological significance and the diversification processes of this diversity remain mostly unknown. Here we report that the ecologically diverse genus Callitriche shows unprecedented intrageneric diversity in meristemoid behavior. While meristemoids in terrestrial species of Callitriche undergo a series of asymmetric divisions before differentiation, those in amphibious species skip the divisions and directly differentiate into stomata. The simple shift in the expression times of two key transcription factors underlies these different patterns. This study provides important insights into the evolution and ecological significance of stomatal patterning.

A molecular timescale for eukaryote evolution with implications for the origin of red algal-derived plastids

In modern oceans, eukaryotic phytoplankton is dominated by lineages with red algal-derived plastids such as diatoms, dinoflagellates, and coccolithophores. Despite the ecological importance of these groups and many others representing a huge diversity of forms and lifestyles, we still lack a comprehensive understanding of their evolution and how they obtained their plastids. New hypotheses have emerged to explain the acquisition of red algal-derived plastids by serial endosymbiosis, but the chronology of these putative independent plastid acquisitions remains untested. Here, we establish a timeframe for the origin of red algal-derived plastids under scenarios of serial endosymbiosis, using Bayesian molecular clock analyses applied on a phylogenomic dataset with broad sampling of eukaryote diversity. We find that the hypotheses of serial endosymbiosis are chronologically possible, as the stem lineages of all red plastid-containing groups overlap in time. This period in the Meso- and Neoproterozoic Eras set the stage for the later expansion to dominance of red algal-derived primary production in the contemporary oceans, which profoundly altered the global geochemical and ecological conditions of the Earth.

Impact Assessment of Urban Pull-factors to cause Uncontrolled Urbanization: Evidence from Pakistan

Uncontrolled urbanization is a global phenomenon, currently sweeping through developing countries like Pakistan. Being the 6th most populous country, its strategic urban locations receive a humongous migrant influx. A prime victim is Hyderabad, Pakistan, the Sindh’s second-largest urban settlement after Karachi that experiences enormous urban problems due to immense urbanization. Hence, the study aims to propose policy implications to curtail Hyderabad’s urbanization. The objective of this study is to measure the impacts of pull-factors in perception of migrants so that the policy implications could be proposed on most responsible factors. For this study, 3 urban Talukas (a district’s subdivision) of district Hyderabad were selected. While a sample of 400 migrants was procured using purposive and snowball sampling techniques for the questionnaire survey. Utilizing a large survey data, the results were obtained using descriptive statistics and multiple regression. Results showed a significant impact of economic and socio-cultural pull-factors like better employment opportunities, higher income probability, job security, easy access to facilities, better outlook and hope for the future, and comfortable and diverse lifestyle in causing immense urbanization. Nevertheless, desire for independence and access to basic social services appeared to have a negative impact. The proposed policy implications on significant pull-factors could be considered as a first step to handle uncontrolled urbanization. Moreover, the study is significant as it particularly contributes to apprehend Hyderabad’s various interrelated urban issues. In general terms, this research can be considered as a role model for high-density third world cities suffering from relatable urban malaise.

Euglenozoa: taxonomy, diversity and ecology, symbioses and viruses.

Euglenozoa is a species-rich group of protists, which have extremely diverse lifestyles and a range of features that distinguish them from other eukaryotes. They are composed of free-living and parasitic kinetoplastids, mostly free-living diplonemids, heterotrophic and photosynthetic euglenids, as well as deep-sea symbiontids. Although they form a well-supported monophyletic group, these morphologically rather distinct groups are almost never treated together in a comparative manner, as attempted here. We present an updated taxonomy, complemented by photos of representative species, with notes on diversity, distribution and biology of euglenozoans. For kinetoplastids, we propose a significantly modified taxonomy that reflects the latest findings. Finally, we summarize what is known about viruses infecting euglenozoans, as well as their relationships with ecto- and endosymbiotic bacteria.

Hind Limb Bone Proportions Reveal Unexpected Morphofunctional Diversification in Xenarthrans

Divergence in forelimb morphology is often associated with functional habits exhibited within the Xenarthra, ranging from terrestrial-digging in armadillos to arboreal-suspension in sloths. We hypothesized that quantitative differences in hind limb form also will be predictive of the diverse lifestyles observed in this small clade. A total of 26 morphofunctional indices were calculated from 42 raw measurements of bone length/width/depth in a sample of N = 76 skeletal specimens (18 species). Index data for each species were categorized by substrate preference and use and then evaluated using a combination of stepwise Discriminant Function Analysis (DFA) and Principal Component Analysis (PCA) to determine significant osteological correlates (traits) among extant taxa. Additionally, character states of the morphometric data were inferred using a recent hypothesis of xenarthran phylogeny. DFA determined 14 distinct morphofunctional indices relating to femur robustness, hip/ankle/limb mechanical advantage, and foot and claw length as the most discriminating features. PCA clearly separated armadillos from sloths in morphospace based on overall robustness versus gracility, as well as proximal versus distal lengths of skeletal elements (including the claws), whereas these characteristics were intermediate in the hind limbs of anteaters and selected armadillos having either a larger greater trochanter or modified foot/claw proportions. Two-toed and three-toed sloths showed further separation from each other in morphospace primarily driven by proportions of their tibia and hind feet despite evidence of convergence for numerous functional traits. Moreover, the majority of the traits measured had significant phylogenetic signal and several of these indicated clear patterns of convergent and divergent evolution in xenarthrans by evaluation of their tip states. Our assessments expand functional interpretations of xenarthran limb form and identify potentially conserved and secondarily modified traits related to fossoriality across taxa, including in three-toed sloths, demonstrate possible morphological trade-offs between digging and climbing habits, and suggest derived traits adapted for arboreal lifestyle and suspensory function.

RESPECT FOR DIVERSITY BASED ON THE VIEWS OF PRE-SERVICE SOCIAL STUDIES TEACHERS

One of the objectives of the social studies course is to train active and democratic citizens, who could tolerate differences. The aim of the present study was to determine the concept of respect for diversity based on the views of pre-service social science teachers and to reveal the cases where there is and there is no respect for diversity. The study was conducted at Firat University, Faculty of Education during the 2018-2019 academic year spring semester. The case study method, a qualitative research design, was used in the study. Convenience sampling method, a purposive sampling method, was also utilized. The study group included 21 pre-service social studies teachers. In the study, the data were collected with a semi-structured interview form and analyzed with the descriptive analysis technique. Based on the study findings, pre-service teachers stated that there was no respect for diversity in religion, ancestry, lifestyle and several other issues. According to the pre-service teachers, the reasons for the lack of respect for diversity were bias, a desire to consider others as inequal, gender differences, ignorance and lack of empathy, and traditional social structure. They defined respect for diversity as respect for differences in religion, language, and race. Article visualizations:

Species Diversity of the Pleurostomatid Ciliate Genus Amphileptus (Ciliophora, Haptoria), With Notes on the Taxonomy and Molecular Phylogeny of Three Species

Amphileptus is one of the largest genera of pleurostomatid ciliates and its species diversity has been reported in various habitats all over the world. In the present work, we review its biodiversity based on data with reliable morphological records. Our work confirms that there are 50 valid Amphileptus species, some of which have a wide range of salinity adaptability and diverse lifestyles. This genus has a high diversity in China but this might be because of the relatively intensive sampling. Phylogenetic analyses based on SSU rDNA sequence data verify the non-monophyly of the genus Amphileptus. Furthermore, two new and one poorly known Amphileptus species, namely A. shenzhenensis sp. n., A. cocous sp. n., and A. multinucleatusWang, 1934, from coastal habitats of southern China were investigated using morphological and molecular phylogenetic methods. These three species are highly similar based on their contractile vacuoles and macronuclear nodules. However, they can be discriminated by details of their living morphology and somatic kineties. We also propose two new combinations, Amphileptus polymicronuclei (Li, 1990) comb. n. (original combination Hemiophrys polymicronucleiLi, 1990) and Amphileptus salimicus (Burkovsky, 1970b) comb. n. (original combination Hemiophrys salimicaBurkovsky, 1970b).

Three nuclear protein-coding genes corroborate a recent phylogenomic model of the Branchiopoda (Crustacea) and provide estimates of the divergence times of the major branchiopodan taxa.

The class Branchiopoda (Crustacea) shows great diversity in morphology and lifestyle among its constituent higher-level taxa: Anostraca, Notostraca, Laevicaudata, Spinicaudata, Cyclestherida and Cladocera. The phylogenetic relationships among these taxa have long been controversial. We sequenced three orthologous nuclear genes that encode the catalytic subunit of DNA polymerase delta and the largest and second-largest subunits of RNA polymerase II in the expectation that the amino acid sequences encoded by these genes might be effective in clarifying branchiopod phylogeny and estimating the times of divergence of the major branchiopodan taxa. The results of phylogenetic analyses based on these amino acid sequences support the monophyly of Branchiopoda and provide strong molecular evidence in support of the following phylogenetic relationships: (Anostraca, (Notostraca, (Laevicaudata, (Spinicaudata, (Cyclestherida, Cladocera))))). Within Cladocera, comparison of the nucleotide sequences of these same genes shows Ctenopoda to be the sister group of Haplopoda + Anomopoda. Three statistical tests based on the present amino acid sequence data-the approximately unbiased test, Kishino-Hasegawa test and weighted Shimodaira-Hasegawa test-tend to refute most of the previous molecular phylogenetic studies on Branchiopoda, which have placed Notostraca differently than here; however, our results corroborate those of one recent phylogenomic study, thus confirming the effectiveness of these three genes to investigate relationships among branchiopod higher taxa. Divergence time estimates calibrated on the basis of fossil evidence suggest that the first divergence of extant branchiopods occurred about 534 Ma during the early Cambrian period and that diversification within the extant branchiopod lineages started in or after the late Permian.

Non-oral Prevotella stepping into the spotlight

Species now affiliated to genus Prevotella have been known for decades as an integral part of human oral cavity microbiota. They were frequently isolated from patients with periodontitis or from dental root canals but also from healthy subjects. With the exception of Prevotella intermedia, they were considered opportunistic pathogens, as they were isolated also from various bacterial abscesses from the head, neck, breast, skin and various other body sites. Consequently, Prevotella were not in the focus of research activities. On the other hand, the four species found in the rumen never caused any disease and seemed early on to be numerous and important part of the rumen ecosystem indicating this genus harbored bacteria with enormously diverse habitats and lifestyles. The purpose of this review is to illustrate the main research themes performed in Prevotella on a path from less noted oral bacteria and from hard to cultivate and study rumen organisms to important mutualistic bacteria in guts of various mammals warranting major research efforts.

Comparative genomics reveals new functional insights in uncultured MAST species

Heterotrophic lineages of stramenopiles exhibit enormous diversity in morphology, lifestyle, and habitat. Among them, the marine stramenopiles (MASTs) represent numerous independent lineages that are only known from environmental sequences retrieved from marine samples. The core energy metabolism characterizing these unicellular eukaryotes is poorly understood. Here, we used single-cell genomics to retrieve, annotate, and compare the genomes of 15 MAST species, obtained by coassembling sequences from 140 individual cells sampled from the marine surface plankton. Functional annotations from their gene repertoires are compatible with all of them being phagocytotic. The unique presence of rhodopsin genes in MAST species, together with their widespread expression in oceanic waters, supports the idea that MASTs may be capable of using sunlight to thrive in the photic ocean. Additional subsets of genes used in phagocytosis, such as proton pumps for vacuole acidification and peptidases for prey digestion, did not reveal particular trends in MAST genomes as compared with nonphagocytotic stramenopiles, except a larger presence and diversity of V-PPase genes. Our analysis reflects the complexity of phagocytosis machinery in microbial eukaryotes, which contrasts with the well-defined set of genes for photosynthesis. These new genomic data provide the essential framework to study ecophysiology of uncultured species and to gain better understanding of the function of rhodopsins and related carotenoids in stramenopiles.

Metagenomic Analyses Expand Bacterial and Functional Profiling Biomarkers for Colorectal Cancer in a Hainan Cohort, China.

This study was conducted for the metagenomic analysis of stool samples from CRC affected individuals to identify biomarkers for CRC in Hainan, the only tropical island province of China. The gut microbiota of CRC patients differed significantly from that of healthy and reference database cohorts based on Aitchison distance and Bray-Cutis distance but there was no significant difference in alpha diversity. Furthermore, at the species level, 68 species were significantly altered including 37 CRC-enriched, such as, Fusobacterium nucleatum, Parvimonas micra, Gemella morbillorum, Citrobacter portucalensis, Alloprevotella sp., Shigella sonnei, Coriobacteriaceae bacterium, etc. Sixty-seven different metabolic pathways were acquired, and pathways involved in the synthesis of many amino acids were significantly declined. Besides, 2 identified antibiotic resistance genes performed well (area under the receive-operation curve AUC = 0.833, 95% CI 58.51-100%) compared with virulence factor genes. The results of the present study provide region-specific bacterial and functional biomarkers of gut microbiota for CRC patients in Hainan. Microbiota is considered as a non-invasive biomarker for the detection of CRC. Gut microbiota of different geographic regions should be further studied to expand the understanding of markers, especially for the China cohort due to diverse nationalities and lifestyles.

Characterization of bacteriophage T7-Ah reveals its lytic activity against a subset of both mesophilic and psychrophilic Aeromonas salmonicida strains.

Aeromonas salmonicida strains cause problematic bacterial infections in the aquaculture industry worldwide. The genus Aeromonas includes both mesophilic and psychrophilic species. Bacteriophages that infect Aeromonas spp. strains are usually specific for mesophilic or psychrophilic species; only a few bacteriophages can infect both types of strains. In this study, we characterized the podophage T7-Ah, which was initially found to infect the Aeromonas salmonicida HER1209 strain. The burst size of T7-Ah against its original host is 72 new virions per infected cell, and its burst time is 30 minutes. It has been found that this phage can lyse both mesophilic and psychrophilic A. salmonicida strains, as well as one strain of Escherichia coli. Its genome comprises 40,153 bp of DNA and does not contain any recognizable toxin or antibiotic resistance genes. The adsorption rate of the phage on highly sensitive bacterial strains was variable and could not be related to the presence or absence of a functional A-layer on the surface of the bacterial strains. The lipopolysaccharide migration patterns of both resistant and sensitive bacterial strains were also studied and compared to investigate the nature of the potential receptor of this phage on the bacterial surface. This study sheds light on the surprising diversity of lifestyles of the bacterial strains sensitive to phage T7-Ah and opens the door to the potential use of this phage against A.salmonicida infections in aquaculture.

“Life space” in the context of psychology of everyday life: To the 130th anniversary of Kurt Lewin

The psychology of everyday life is one of the actively developing areas of modern Russian psychology. The leading theoretical and methodological sources of its development are history, anthropology and sociology of everyday life at the general scientific level of knowledge. Sources at the specific scientific level are the psychopathology of everyday life by Sigmund Freud, the field theory of Kurt Lewin, and the psychological concept of Hans Thomae. The demand for the ideas of Lewin is determined today by transformations of modernity, bringing to the fore the issues of socio-cultural dynamics and polymotivation of an individual, the clash of values and the subject’s conscious choice of a life path, formation of identity in changing life situations, and diversity of lifestyles in a complicated society. Everyday life is multi-layered, variegated, and heterogeneous. It presents the dynamics of the visible and invisible, real and surreal, modern and archaic. To detect gradual and imperceptible changes, it is often necessary to change the methodological toolkit. Lewin’s construct of “life space” in the context of the psychology of everyday life allows one to configure various aspects of human development in a situation of sociocultural transformations where social space can be both supportive and suppressive, and the spheres of private and public, personal and professional, mix. This results in a loss of normative boundaries. Cultural-psychological time in a common geographic space flows differently for people and communities, which not only creates risks of potential conflicts by drawing fault lines in the social fabric, but also poses the problem of a description language capable of combining nomothetic (revealing trends and patterns) and idiographic (directed analysis of unique cases) discourses. Lewin solved similar methodological and socio-political problems. It seems that his approach is very productive for the diagnosis and treatment of cultural and psychological trauma in Russian society. In the article, it is emphasized that the strategy of Lewin’s “action research” is close to the social constructivism of the Russian intellectual tradition, which makes it possible to comprehend the work of a psychologist as a solution to the problems of humanizing society and the education system.

Mitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen Corynespora cassiicola

Corynespora cassiicola, the causal agent of an extensive range of plant diseases worldwide, is a momentous fungus with diverse lifestyles and rich in intraspecies variations. In the present study, a total of 56 mitochondrial genomes of C. cassiicola were assembled (except two available online) and analyzed, of which 16 mitogenomes were newly sequenced here. All these circular mitochondrial DNA (mtDNA) molecules, ranging from 39,223 bp to 45,786 bp in length, comprised the same set of 13 core protein-coding genes (PCGs), two rRNAs and 27 tRNAs arranged in identical order. Across the above conserved genes, nad3 had the largest genetic distance between different isolates and was possibly subjected to positive selection pressure. Comparative mitogenomic analysis indicated that seven group I (IB, IC1, and IC2) introns with a length range of 1013–1876 bp were differentially inserted in three core PCGs (cox1, nad1, and nad5), resulting in the varied mitogenome sizes among C. cassiicola isolates. In combination with dynamic distribution of the introns, a well-supported mitogenome-wide phylogeny of the 56 C. cassiicola isolates revealed eight phylogenetic groups, which only had weak correlations with host range and toxin class. Different groups of isolates exhibited obvious differences in length and GC content of some genes, while a degree of variance in codon usage and tRNA structure was also observed. This research served as the first report on mitogenomic comparisons within C. cassiicola, and could provide new insights into its intraspecific microevolution and genetic diversity.