Insect Diversity Research Articles

Insect Flight: State of the Field and Future Directions.

The evolution of flight in an early winged insect ancestral lineage is recognized as a key adaptation explaining the unparalleled success and diversification of insects. Subsequent transitions and modifications to flight machinery, including secondary reductions and losses, also play a central role in shaping the impacts of insects on broadscale geographic and ecological processes and patterns in the present and future. Given the importance of insect flight, there has been a centuries-long history of research and debate on the evolutionary origins and biological mechanisms of flight. Here, we revisit this history from an interdisciplinary perspective, discussing recent discoveries regarding the developmental origins, physiology, biomechanics, and neurobiology and sensory control of flight in a diverse set of insect models. We also identify major outstanding questions yet to be addressed and provide recommendations for overcoming current methodological challenges faced when studying insect flight, which will allow the field to continue to move forward in new and exciting directions. By integrating mechanistic work into ecological and evolutionary contexts, we hope that this synthesis promotes and stimulates new interdisciplinary research efforts necessary to close the many existing gaps about the causes and consequences of insect flight evolution.

Species traits to guide moth conservation in anthropogenic regions: A multi‐species approach using distribution trends in Flanders (northern Belgium)

Abstract Insect abundance and diversity appear to decline rapidly in recent decades, garnering significant media attention, and hence raising public awareness. Macro‐moths—a species‐rich and ecologically diverse insect group—face severe declines, particularly in urbanised and intensively farmed areas in NW Europe. Flanders is a highly anthropogenic region, serving as a case study where the impact on macro‐moths of stressors like intensive agriculture, industrialisation and urbanisation has been quantified through a recently compiled Red List. Here, for 717 macro‐moth species, we calculated relative changes in distribution area between a reference period (1980–2012) and the subsequent period (2013–2022). By correlating these species‐specific trends with 10 key ecological and life history traits, we calculated more general Multi‐Species Change Indices (MSCIs). These MSCIs showed that species associated with wet biotopes and heathlands declined on average by 20%–25%, while (sub)urban species increased by more than 60%. Species feeding on lichens or mosses increased by 31%, while grass‐feeding species decreased by 20%. Both very small (+34%) and very large species (+15%) increased, whereas medium‐sized species decreased by 5%. Monophagous (+17%), migrant (+88%) and colour‐invariable species (+5%) increased, while colour‐variable species decreased (−8%). Finally, Holarctic (−21%) and Palaearctic species (−5%) decreased, while Mediterranean (+27%) and Western‐Palaearctic species (+9%) increased. Our trait‐based approach identifies key threats and mitigation strategies for moths in anthropogenic regions, offering evidence‐based insights for crafting efficient management recommendations and informed conservation policies to safeguard moth communities.

Bug city life: Public acceptance of urban insect-friendly meadows in Germany, Austria, and Switzerland

Reports of continuing declines in some insect populations have raised concerns and calls for action to protect insects. The establishment of insect-friendly meadows in urban areas provides an opportunity to conserve both insect and plant diversity. However, public acceptance can be an obstacle to the implementation of such meadows, for example, due to competing land use interests and the perception that tall meadows are visually unattractive. To address these concerns and align urban green spaces with both public preferences and environmental needs, a systematic understanding of the factors influencing public acceptance of meadows is essential. To this end, a representative online survey was conducted in Germany, Austria, and Switzerland with 899 participants to assess the acceptance of insect-friendly meadows in urban areas. The study shows significant support for insect-friendly meadows across all subgroups and within each country-specific subgroup. The factor that most influences their acceptance is the recognition of the ecological benefits of these meadows. It is therefore highly recommended to prioritize public awareness campaigns that highlight the complex relationship between plant and insect diversity. Such efforts can contribute to a better understanding of the benefits of insect-friendly meadows for biodiversity, thereby increasing public support for these conservation measures. Additionally, considerations related to perceived aesthetics, appreciation of an increase in insect abundance, preference for public funding, and a general sense of safety around these meadows should inform the development and communication strategies for insect-friendly green spaces, as they significantly influence public acceptance. These design and perception aspects of meadows were shown to have a stronger influence on the acceptance of meadows than individual, attitudinal characteristics such as environmental attitudes or attitudes toward insect decline. While this study provides insights into the general public acceptance of insect-friendly meadows, more research is needed to understand the influence of the specific local context on meadow acceptance.

New aquatic insects from the Miocene of Australia with notes on the ecology and ontogeny of a new species of Chaoborus (Diptera, Chaoboridae)

AbstractWe describe a diverse aquatic insect assemblage from McGraths Flat, a Miocene Lagerstätte in central New South Wales, Australia that includes representatives of Sialidae, Limoniidae, Chironomidae and Chaoboridae. The aquatic insect fossils from this deposit consist predominantly of larvae. These include a new species of phantom midge (Chaoborus, Chaoboridae), three morphotypes of non‐biting midges (Chironomidae), one morphotype of cranefly (Limoniidae) and one morphotype of alderfly (Sialidae). The large number of fossil specimens enabled us to study the ontogeny of the new midge species. We discerned growth rates in fossil larvae, using morphometry of all four instars of Chaoborus. The simultaneous presence of taxa associated with still water and taxa associated with flowing water supports the hypothesis that McGraths Flat was deposited in an isolated water body (oxbow lake/billabong) with influence from a river during high water events.

Adapting to changing methodology in a long‐term experiment

AbstractLong‐term experiments are critical for understanding ecological processes, but their management comes with unique challenges. As time passes, projects may encounter unavoidable changes due to external factors, like availability of materials, affecting aspects of their research methodology. At the Kellogg Biological Station Long‐Term Ecological Research Site, one of the many National Science Foundation‐funded long‐term research stations, a three‐decade project recently experienced a supply‐chain‐induced change in insect sampling methodology in their lady beetle observation study. Since 1989, lady beetles (Coleoptera: Coccinellidae) have been sampled weekly over the growing season using yellow sticky cards. In 2021, the original sticky traps were discontinued by the manufacturer and replaced with a similar, but not identical trap. We conducted a 3‐year study while the new traps were phased in to examine how the trap change would impact the observed biodiversity patterns at the site. We examined community metrics and individual taxa captures to examine within‐year and between‐year differences in performance between the card types. Overall, we noted several small but statistically detectable differences in capture patterns between the two trap types. After accounting for other sources of variation, we observed a difference in Shannon diversity of insects captured on the two card types, but not richness or abundance, for the overall insect community. Yet, these differences were dwarfed by the magnitude of difference observed between years within card types. For individual taxa, similar patterns held: between trap differences could be detected statistically, but the number of differences in capture rate between trap types was less than the number of differences observed for the same trap, between years. Thus, we conclude that while subtle changes in methodology could impact data produced in long‐term experiments; in this case, the magnitude of this change is smaller than other factors such as time and plant treatment. However, if sustained changes in the capture rates of focal taxa are observed, future data users may use our observations to specifically quantify and correct for these shifting patterns related to the protocol change.

Skimming the skaters: genome skimming improves phylogenetic resolution of Halobatinae (Hemiptera: Gerridae)

Abstract Gerromorpha Popov, 1971 is a fascinating and diverse insect lineage that evolved about 200 Mya to spend their entire life cycle on the air–water interface and have since colonized all types of aquatic habitats. The subfamily Halobatinae Bianchi, 1896 is particularly interesting because some species have adapted to life on the open ocean—a habitat where insects are very rarely found. Several attempts have been made to reconstruct the phylogenetic hypotheses of this subfamily, but the use of a few partial gene sequences recovered only a handful of well-supported relationships, thus limiting evolutionary inferences. Fortunately, the emergence of high-throughput sequencing technologies has enabled the recovery of more genetic markers for phylogenetic inference. We applied genome skimming to obtain mitochondrial and nuclear genes from low-coverage whole-genome sequencing of 85 specimens for reconstructing a well-supported phylogeny, with particular emphasis on Halobatinae. Our study confirmed that Metrocorini Matsuda, 1960, is paraphyletic, whereas Esakia Lundblad, 1933, and Ventidius Distant, 1910, are more closely related to Halobatini Bianchi, 1896, than Metrocoris Mayr, 1865, and Eurymetra Esaki, 1926. We also found that Ventidius is paraphyletic and in need of a taxonomic revision. Ancestral state reconstruction suggests that Halobatinae evolved progressively from limnic to coastal habitats, eventually attaining a marine lifestyle, especially in the genus Halobates Eschscholtz, 1822, where the oceanic lifestyle evolved thrice. Our results demonstrate that genome skimming is a powerful and straightforward approach to recover genetic loci for robust phylogenetic analysis in non-model insects.

New record of species Anaulacus Aephnidius ampliusculus (Coleoptera: Carabidae: Masoreini ) in Iraq

A new record of species Anaulacus Aephnidius ampliusculus in Iraq which belong to family Carabidae in order Coleoptera when study the diversity of insects in Canaan forests in Diyala governorate. With highest density recorded in May and September.

Evaluation of the Restoration Effects of Rooftop Greening Areas Created by Applying an Ecological Restoration Method

Green roofs provide various ecosystem services, including habitats for diverse organisms in urban areas where natural space is very scarce. This study aims to evaluate the restoration effects of green roofs created by applying an ecological restoration method to reinforce habitat function. The reference ecosystem selected for the ecological restoration of a roof was a Korean red pine stand established on Mt. Bulam, considering the soil depth, light, moisture conditions, etc., compared with the roof’s environmental conditions. Ecological restoration was carried out by planting two-year-old pine seedlings and scattering surface soil collected from the pine stands. The pine trees showed geometrical growth in height and diameter and a high water-use efficiency. The ecological restoration of the green roofs demonstrated an ecological function for improving the urban climate through the mitigation of the urban heat island effect and high productivity, showing different functions depending on the greening method. The composition and diversity of vegetation and insects at the ecological restoration site were similar to those at the reference site, whereas the landscape architecture sites, which were created using the landscape architecture method, were different from the reference site. These results confirm that applying the ecological restoration method for rooftop greening resulted in better biological habitat function than applying existing landscape architecture methods.

Habitat Improvement In Effort To Conservation Insect Diversity And Natural Enemies On Potato Cultivation

This study aimed to determine the habitat approach on potato cultivation to increase diversity of insects. Habitat improvement design by agricultural system and planting system on different season. This study consisted of two experiments. The first done at planting season from March to July (dry season) and the second done at planting season from October to January (rainy season). Research designed by Split plot design with main plot are farming systems (inorganic farming and organic farming) and the subplot are the design of cropping system (sole potato; potato and cabbage; potato and mustard; potato and onion). The data was analyzed by ANOVA and Tukey test. The result showed that planting season from March to July (dry season) as well as the planting season from October to January (rainy season) showed that insect diversity index, predator population and parasitoid population were higher in organic farming compared to inorganic farming systems. The design of cropping system significantly affect insect diversity index,natural enemies population such as predator Coccinelids and parasitoid Braconid wasp and M. persicae population. In organic farming, intercropping potato with cabbage found the highest insect diversity index and the highest population of parasitoid Braconid wasp and the lowest population of M. persicae. The highest population of predator Coccinelids found at interropping potato with mustard. Combined analysis of the planting on dry season and rainy season showed that insect diversity index and population of parasitoid Baraconid wasp not significantly difference between planting on dry planting and planting on rainy season. On the other hand, population of predator Coccinellids and M. persicae significantly more higher at planting on dry season than planting on rainy season.

The positive impact of honeybee activity on fennel crop production and sustainability

This study investigates the ecological interaction between honeybees (Apis mellifera) and fennel (Foeniculum vulgare) plants, examining the mutual benefits of this relationship. Field experiments conducted in Egypt from December 2022 to May 2023 recorded diverse insect pollinators attracted to fennel flowers, especially honeybees. Assessing honeybee colonies near fennel fields showed improvements in sealed brood (357.5–772.5 cells), unsealed brood (176.3–343.8 cells), pollen collection (53.25–257.5 units), honey accumulation (257.5–877.5 units), and colony strength (7.75–10) over three weeks. Fennel exposure explained 88–99% of variability in foraging metrics. Comparing open versus self-pollinated fennel revealed enhanced attributes with bee pollination, including higher flower age (25.67 vs 19.67 days), more seeds per umbel (121.3 vs 95.33), bigger seeds (6.533 vs 4.400 mm), heavier seeds (0.510 vs 0.237 g/100 seeds), and increased fruit weight per umbel (0.619 vs 0.226 g). Natural variation in seed color and shape also occurred. The outcomes demonstrate the integral role of honeybees in fennel agroecosystems through efficient pollination services that improve crop productivity and quality. Fennel provides abundant nutritional resources that bolster honeybee colony health. This research elucidates the symbiotic bee-fennel relationship, underscoring mutualistic benefits and the importance of ecological conservation for sustainable agriculture.

Keanekaragaman Serangga di Kebun Teh PTPN VIII di Goalpara Kabupaten Sukabumi

Insects have an important role in agriculture as pollinators, predators and decomposers, but can also be pests that damage plants and reduce production yields. If farmers can identify pests quickly and precisely based on their symptoms, negative impacts can be minimized. This research aimed to identify insect diversity in the PTPN VIII tea garden in Goalpara, Cisarua Village, Sukaraja District, Sukabumi Regency, West Java, and was conducted from November to December 2023. The method used was descriptive with an exploratory approach. Data was collected through direct observation using yellow traps and pitfall traps at three locations at different heights. As a result, 12 orders and 29 families of insects were found, belonging to Hymenoptera, Entomobryomorpha, Diptera, Araneae, Dermaptera, Orthoptera, Coleoptera, Blattaria, Hemiptera, Lepidoptera, Psocoptera, and Blattodea. The diversity indices at locations I, II, and III were 2.48; 2.23; and 2.17, respectively; whereas the dominance indices were 0.11; 0.16; and 0.13, respectively. In conclusion, insect diversity in the Goalpara PTPN VIII tea garden was classified as moderate, with a low level of dominance, indicating a balanced and stable ecosystem. This research is useful for providing information about the diversity and abundance of insects on the PTPN VIII Goalpara Tea Plantation as well as providing knowledge to farmers about the role of insects on the plantation, including insects that have the potential to act as pests, predators, parasitoids and pollinators.

Pollinator insects in the Mount Tumpa Forest Park, Manado, North Sulawesi, Indonesia

Abstract. Koneri R, Langoy MD, Nangoy MJ, Wakhid. 2024. Pollinator insects in the Mount Tumpa Forest Park, Manado, North Sulawesi, Indonesia. Biodiversitas 25: 2415-2426. This study aimed to analyze the diversity and distribution of pollinator insects in forest, forest edge, shrub, and agricultural land around the Mount Tumpa Forest Park, Manado, North Sulawesi, Indonesia, using the scan sampling method. Data analysis included species abundance, species dominance, richness index, diversity index, species evenness index, correlation between environmental factors, species diversity, and composition of pollinator insect species among habitats. Therefore, 17 families were reported, comprising 103 species and 3,665 individuals. The family with the highest number and most frequently encountered was Nymphalidae (25 species). Furthermore, the highest species richness and diversity indices were found in the forest edge habitat. Environmental factor analysis indicated that agricultural land habitat was characterized by high air temperature and low humidity. The species distribution found three patterns: clustered, regular, and random. The results showed that the forest edge habitat had the highest diversity due to complex vegetation structure and environmental factors supporting the survival of pollinator insects.

Modelling decisions and density dependence in monarch butterflies: A comment on Meehan and Crossley (2023)

Abstract Decisions about which processes to include in a population model can have substantial impact on estimates of population trends and extinction risk. This is particularly important for species of conservation concern, whose conservation status is decided in part on the basis of these models. In their recent paper, Meehan and Crossley (2023) Insect Conservation and Diversity, 16, 566–573 revisit a time series of overwintering monarch butterfly abundances, which previous assessments had characterised as rapidly declining and at risk of extinction. The authors, in contrast, reported no evidence for declines in the past 10 years and characterised extinction risk as low. A primary reason for the difference between these conclusions was that Meehan and Crossley used a more complex model that included a parameter for density dependence. While negative density dependence may play a role in monarch population regulation, there are a variety of unresolved issues with how and if density dependence should be included in models of monarch populations, including widely known issues with separating observation error from density dependence in noisy time series and taxon‐specific issues with fitting models to data surveyed every fourth generation and pooled at continental scales. These issues make the conclusions of Meehan and Crossley about monarch population viability much less robust than implied in their article. They do not provide convincing evidence that density dependence reduces extinction risk in monarch butterflies. Our commentary supports their general conclusion that population viability projections depend on model assumptions, but the ways in which monarch butterfly populations are regulated remains an open question.

ECOLOGY OF THE NILGIRI LARGE BURROWING SPIDER HAPLOCLASTUS NILGIRINUS POCOCK, 1899 (ARANEAE, THERAPHOSIDAE) OF NILGIRIS, INDIA

The Nilgiri large burrowing spider, Haploclastus nilgirinus Pocock, 1899, an endemic tarantula in the Nilgiris Region of India, is critical for conservation yet poorly understood in terms of its ecology and habitat. This study addresses this gap by examining the nesting habits, tree-hole dynamics, and prey diversity of the species in Coonoor, The Nilgiris, specifically within tea plantations and fruit orchards. Observations focused on seven pear trees serving as nesting sites, with detailed measurements of nesting holes, including outer and inner circumference, depth, trunk base, ground-based height (gbh), and height from the ground. Data were collected over four consecutive months, both morning and evening. Insect diversity in the orchard was also assessed through counts and diversity indices, revealing variability among trees. The seventh tree had the highest spider population, with 13 spiders in 10 nests. Insect orders such as Blattodea, Orthoptera, Diptera, Hemiptera, and Lepidoptera were most prevalent around the seventh tree’s nests. Across all trees, a total of 25 spiders in 21 nests were documented, with variations in hole size and other tree measurements. This research provides crucial insights into the nesting habits, tree-hole dynamics, and prey diversity of this species, informing conservation efforts for this vulnerable species. As H. nilgirinus faces threats from habitat destruction, climate change, and illegal trade, understanding its ecological needs is vital for developing effective conservation strategies and sustaining ecosystem health.

Species Richness and Diversity of Insects in an Argo-ecosystem in Bhabar Region of Uttarakhand

The aim of the presented study was to estimate the species richness and diversity of insects were investigated in an agro-ecosystem in Bhabar region of Uttarakhand. In total, 992 individuals were collected representing 7 orders (Lepidoptera, Coleoptera, Hemiptera, Hymenoptera, Orthoptera, Diptera and Odonata), 30 families and 91 species that included herbivores, predators, omnivores and saprophages. The most dominant order was Lepidoptera with a relative abundance of (46.15%) and, the least was Diptera (6.59%). The five most abundant families by numbers of individuals were Pieridae (14.8%), Nymphalidae (13.9%), Lycaenidae (6.1%), Papilionidae (5.9%), and Libellulidae (5.4%). The five most diverse families by species were Nymphalidae (13), Pieridae (9), Lycaenidae (8), Papilionidae (7), and Libellulidae (6). The diversity index showed significant Diversity (Hʹ=1.832), Evenness (E=0.9449) and Margalef species richness (d=2.076) of insect fauna. Diversity indices of insect orders showed that Lepidoptera was the most diverse (Hʹ=1.641), Dipterans had highest Evenness (E=0.9449), and Coleopterans had the maximum species richness as per Margalef’s Index (d=2.056). There were 720 individuals of insect pollinators visitors were observed, which belonged to 62 species in 4 orders (Lepidoptera, Coleoptera, Hymenoptera, and Hemiptera).

Development of insects in newly established grassland over six years: Increased conservation benefits through diverse plant communities

Agri-environmental schemes seek to mitigate the loss of biodiversity by subsidizing the restoration of grassland in Europe. However, little is known about the colonization of newly established grasslands by insects, especially over periods exceeding 1–2 years. Therefore, we investigated the development of species richness, abundance and species assemblages of wild bees, syrphids, butterflies, orthopterans and true bugs in two types of newly established grassland strips in an agricultural landscape in Austria over a six-year time span. We compared currently subsidized plant-species poor grassland strips (SG; Ecological Focus Areas) with newly established grassland strips (NG) that were created with a diverse, regionally adapted seed mixture, to find out whether diverse seed mixtures can ensure lasting improvements in the insect species richness of grassland strips over a 6-year period. We used permanent seminatural grasslands (old grassland OG) as reference habitat. From the first year after establishment, we found no differences in syrphid assemblages between OG and NG. In contrast, true bug assemblages in NG differed significantly from those in OG still five years after establishment. Wild bee, butterfly and orthopteran species composition differences between grassland strips (NG, SG) and permanent grasslands (OG) were decreasing over time. However, we found no evidence of a successional change in insect species assemblages in NG and SG. NG promoted a significantly higher wild bee richness and equivalent syrphid, butterfly, orthoptera and true bug richness compared with OG. Over the study period, NG showed sustained insect diversity, outperforming plant species-poor SG in promoting wild bee, syrphid and true bug richness. Therefore, we suggest that diverse and regional seed mixtures could enhance the effectiveness of grassland restoration for insect conservation and could make re-sowing within the first 6 years after establishment unnecessary.

The genetic determination of alternate stages in polyphenic insects.

Molt-based transitions in form are a central feature of insect life that have enabled adaptation to diverse and changing environments. The endocrine regulation of these transitions is well established, but an understanding of their genetic regulation has only recently emerged from insect models. The pupal and adult stages of metamorphosing insects are determined by the stage specifying transcription factors broad-complex (br) and Ecdysone inducible protein 93 (E93), respectively. A probable larval determinant, chronologically inappropriate metamorphosis (chinmo), has just recently been characterized. Expression of these three transcription factors in the metamorphosing insects is regulated by juvenile hormone with ecdysteroid hormones, and by mutual repression between the stage-specific transcription factors. This review explores the hypothesis that variations in the onset, duration, and tissue-specific expression of chinmo, br, and E93 underlie other polyphenisms that have arisen throughout insects, including the castes of social insects, aquatic stages of mayflies, and the neoteny of endoparasites. The mechanisms that constrain how chinmo, br, and E93 expression may vary will also constrain the ways that insect life history may evolve. I find that four types of expression changes are associated with novel insect forms: (1) heterochronic shift in the turnover of expression, (2) expansion or contraction of expression, (3) tissue-specific expression, and (4) redeployment of stage-specific expression. While there is more to be learned about chinmo, br, and E93 function in diverse insect taxa, the studies outlined here show that insect stages are modular units in developmental time and a substrate for evolutionary forces to act upon.

New Genera and Species of Trigonidiidae (Orthoptera: Grylloidea) from the Mid-Cretaceous of Myanmar with a Redescription of Birmaninemobius hirsutus.

The abundance of insects in Burmese amber illustrates a highly diverse insect community from the mid-Cretaceous period; yet, records of crickets (Grylloidea) are notably scarce. In this study, we describe two new genera with three new species, Palaeotrigonidium concavoculus gen. et sp. nov., Palaeotrigonidium defectivus sp. nov., and Tricalcaratus longilineus gen. et sp. nov., based on three specimens collected in north Myanmar. These new species can be placed within the Trigonidiidae (Orthoptera: Grylloidea) by their triangular head, compound eyes that protrude in dorsal view, and a body entirely covered with robust setae, particularly noticeable in the head and pronotum; however, subfamily assignments are not possible. Another known species, Birmaninemobius hirsutus, Xu et al., 2020, from Myanmar amber is redescribed based on a new specimen and a recheck of the holotype.

Inventory and Analysis of Diversity of Insect on Shallot Cultivation in Saruran Hamlet, Enrekang Regency

Shallots (Allium ascalonicum L.) are a highly popular plant both as a culinary spice and herbal medicine. Due to their economic benefits, many farmers cultivate them. Saruran Hamlet, located in Anggareja District, Enrekang Regency, is one of the areas where shallots are intensively cultivated. Farmers face a significant problem with high pest infestation rates. To manage this, chemical pest control using insecticides is employed. However, insecticides are toxic and increasingly expensive. To reduce pesticide use, pest control using light traps was implemented through the "electricity to the fields" program by the local PLN. The use of light traps is suspected to have a negative impact on the balance of biotic ecosystem components, thus necessitating a study on this matter. Therefore, the aim of this study is to inventory and analyze the diversity of insect species in shallot cultivation areas in Saruran Hamlet, Enrekang Regency. This research employed a survey method by collecting insects trapped in farmers' light traps. The identification and counting of insect species were conducted in the FMIPA UNM Laboratory. Based on the collected data, the Shannon-Wiener diversity index (H’) was analyzed. The results showed that the total number of trapped insect individuals was 1,332, consisting of 27 species from 8 orders. The Shannon-Wiener diversity index (H’) was found to be 2.20, which falls into the moderate category.

Minimally destructive hDNA extraction method for retrospective genetics of pinned historical Lepidoptera specimens

The millions of specimens stored in entomological collections provide a unique opportunity to study historical insect diversity. Current technologies allow to sequence entire genomes of historical specimens and estimate past genetic diversity of present-day endangered species, advancing our understanding of anthropogenic impact on genetic diversity and enabling the implementation of conservation strategies. A limiting challenge is the extraction of historical DNA (hDNA) of adequate quality for sequencing platforms. We tested four hDNA extraction protocols on five body parts of pinned false heath fritillary butterflies, Melitaea diamina, aiming to minimise specimen damage, preserve their scientific value to the collections, and maximise DNA quality and yield for whole-genome re-sequencing. We developed a very effective approach that successfully recovers hDNA appropriate for short-read sequencing from a single leg of pinned specimens using silica-based DNA extraction columns and an extraction buffer that includes SDS, Tris, Proteinase K, EDTA, NaCl, PTB, and DTT. We observed substantial variation in the ratio of nuclear to mitochondrial DNA in extractions from different tissues, indicating that optimal tissue choice depends on project aims and anticipated downstream analyses. We found that sufficient DNA for whole genome re-sequencing can reliably be extracted from a single leg, opening the possibility to monitor changes in genetic diversity maintaining the scientific value of specimens while supporting current and future conservation strategies.