Diverse Taxa Research Articles

Tetrodotoxin, fungal pathogen infection, and bacterial microbiome associations are variable in the skin microecosystems of two Taricha newt species

A diverse metabolome exists on amphibian skin that mediates interactions between hosts and skin microbiomes. Tetrodotoxin is one such metabolite that occurs across a variety of taxa, and is particularly well studied in newts of the genus Taricha that are susceptible to infection with chytrid fungi. The interaction of tetrodotoxin with the skin microbiome, including pathogenic fungi, is not well understood, and here we describe these patterns across 12 populations of Taricha granulosa and T. torosa in Washington, Oregon, and California. We found no correlation of TTX and Batrachochytrium dendrobatidis (Bd) infection in either T. granulosa or T. torosa, a pattern inconsistent with a previous study. In addition, TTX, but not Bd, was significantly correlated with the skin microbiome composition in T. granulosa. In T. torosa, however, Bd, but not TTX, was correlated with the skin microbiome structure. The relationship between TTX and skin microbiome composition differed between species, with significant correlations observed only in T. granulosa, which exhibited higher TTX concentrations. We also detected significantly higher abundances of bacterial taxa (e.g., Pseudomonadaceae) associated with TTX production in newts with higher skin TTX. These taxa (ASVs matching Aeromonas, Pseudomonas, Shewanella, and Sphingopyxis) were associated with all body sites of previously sampled T. granulosa, but not found in soil samples. Our results suggest that toxins can shape the newt skin microbiome and may influence pathogen infection through indirect mechanisms, as TTX showed no direct inhibition of Bd or B. salamandrivorans growth.

Phenological responses to climate change across taxa and local habitats in a high‐Arctic arthropod community

AbstractClimate change has led to pronounced shifts in phenology, varying across taxa. The Arctic is experiencing particularly rapid warming, but long‐term data on phenological changes are rare in this region, especially for arthropods—a diverse taxonomic group that form important links to other trophic levels. Understanding the environmental drivers of arthropod phenological variation is necessary for predicting future trends across taxa and habitats to climate change. Here, we analyze temporal trends and climate associations in arthropod phenology using 25 years of standardized monitoring data from four habitat types in high‐Arctic Greenland. We observed earlier peak activity in the arthropod community, with responses varying considerably among families and habitats. Snowmelt timing was a key driver of peak activity, especially for late‐active taxa, while temperature was a less important driver, but arthropods generally exhibited earlier activity with warming. Responses in the duration of activity were more complex, with family‐ and habitat‐specific responses to climate variation. Notably, taxa in habitats with late snowmelt responded strongly to snowmelt timing, while those in the pond habitat responded strongly to temperature. Mixed feeders and parasitoids showed rapid peak phenological shifts to earlier snowmelt and warming; however, mixed feeders shortened their activity periods, while parasitoids extended theirs. Our findings highlight the complexity of arthropod community phenological responses to climate change, with potential implications for trophic interactions dependent on temporal overlap. By analyzing phenological metrics across entire activity seasons for taxa with different functional and life‐history traits, we identify general trends and consistent patterns that enhance our understanding of arthropod responses to climate change.

Intraspecific gene regulation in cis- and trans.

Changes in gene expression underlie much of evolution and occur via either cis-acting mutations, which lie near the affected gene and act in a context specific manner, or trans-acting mutations, which may be far from the affected gene and act through diffusible molecules such as transcription factors. A commonly held view is that most expression variation within species is controlled in trans- while expression differences between species are largely controlled in cis-. Here, we summarize recent intraspecific gene regulation studies and find, contrary to this widely held view, that many studies in diverse taxa have revealed a large role for cis-acting mutations underlying expression variation within species. A review of the existing literature also shows that preparations using whole organisms rather than individual tissues may be biased towards identifying trans-regulation. Moreover, we note several examples of predominantly cis-acting regulation in recently diverged populations adapted to different environments. We highlight the challenges of drawing general conclusions from comparisons among studies that use different methodologies and we offer suggestions for studies that will address outstanding questions concerning the evolution of gene regulation.

Parasitofauna of Dolly Varden Salvelinus malma complex in Kamchatka

Systematized information on the species composition of parasites of S. malma complex in the water bodies of Kamchatka based on own and literature data is provided. Presented taxonomic list of parasites gives information about localization, distribution, host range, findings with indication of water body, malmoid form, infestation indices and authors of findings for each species. The parasitofauna of Dolly Varden in Kamchatka includes 65 species from 3 kingdoms, 10 types, 15 classes, 25 orders and 42 families. The most diverse taxa are trematodes (16 species), nematodes (10), myxosporidia (10), cestodes (8) and acanthocephalans (7). The basis of the parasitofauna of Dolly Varden in Kamchatka is formed by freshwater species (47), species with a complex life cycle (50), using fish as definitive hosts (38), having Holarctic and circumpolar distribution (29), being a part of the Arctic freshwater and Pacific piedmont faunal complexes (33), specific to fish of the genus Salvelinus, family Salmonidae and the order Salmoniformes (39). The high species and ecological diversity of Dolly Varden parasites is due to its ubiquitous distribution in the region and its complex population structure, including several ecotypes and forms that differ in life strategy, diet, and habitats.

ACCUMULATION OF TRACE ELEMENTS IN SOIL AND FAUNA WITHIN A SITE HISTORICALLY CONTAMINATED WITH COAL COMBUSTION RESIDUES.

Legacy contaminants tied to energy production are a worldwide concern. Coal combustion residues (CCRs) contain high concentrations of potentially toxic trace elements such as arsenic (As), mercury (Hg), and selenium (Se), which can persist for decades after initial contamination. CCR disposal methods, including aquatic settling basins and landfills, can facilitate environmental exposure through intentional and accidental releases. Wildlife exposed to CCRs can experience numerous deleterious effects, such as on development, reproduction, and survival. In the current study, we quantified and compared concentrations of As, Hg, Se, and strontium (Sr) within soils and target fauna (three vertebrate and three invertebrate taxa) from a CCR-contaminated site and a reference site within the U.S. Department of Energy's Savannah River Site, South Carolina, USA. Our objectives were to 1) compare current concentrations of tested elements in soil and resident fauna to levels from our reference site, 2) assess natural attenuation of elements in soils by comparing current concentrations to historic levels, and 3) evaluate the biomagnification potential of the elements measured via body burden and trophic position correlations among fauna. Element concentrations were higher in contaminated soils than reference soils; however, concentrations in 2022 were unchanged from concentrations measured in 2003, suggesting no natural attenuation of tested elements. Additionally, target fauna had elevated As, Se, and Sr levels in comparison to reference samples. A positive correlation was observed in southern toads between Sr concentrations and trophic position, as assessed by nitrogen stable isotope ratios, suggesting potential for biomagnification of Sr within our study system. Collectively, our results demonstrate that legacy contaminants are still present and bioaccumulate in a diversity of taxa in a CCR-contaminated site that has not received effluents in over 50 years, suggesting monitoring programs in CCR-contaminated sites should be maintained long term in the absence of remediation.

Integrating hybridization and introgression into host-parasite epidemiology, ecology, and evolution.

Hybridization and introgression between host species or between parasite species are emerging challenges for human, plant, and animal health, especially as global trends like climate change and urbanization increase overlap of species ranges. This creates opportunities for heterospecific crosses between diverged taxa that could generate novel host and parasite genotypes with unique traits (e.g., transmission rate, virulence, susceptibility, and resistance) compared with their parental taxa. However, there seems to be slow appreciation of this biological phenomenon in empirical and theoretical approaches to host-parasite interactions. This limits our understanding of the effects of hybridization on epidemiology, ecology, and evolution. Here, we address some pressing questions regarding the emergence and relevance of eukaryotic hybrid genotypes for disease dynamics.

Building a reliable 16S mini-barcode library of wild bees from Occitania, south-west of France.

DNA barcoding and metabarcoding are now powerful tools for studying biodiversity and especially the accurate identification of large sample collections belonging to diverse taxonomic groups. Their success depends largely on the taxonomic resolution of the DNA sequences used as barcodes and on the reliability of the reference databases. For wild bees, the barcode sequences coverage is consistently growing in volume, but some incorrect species annotations need to be cared for. The COI (Cytochrome Oxydase subunit 1) gene, the most used in barcoding/metabarcoding of arthropods, suffers from primer bias and difficulties for covering all wild bee species using the classical Folmer primers. We present here a curated database for a 250 bp mini-barcode region of the 16S rRNA gene, suitable for low-cost metabarcoding wild bees in applications, such as eDNA analysis or for sequencing ancient or degraded DNA. Sequenced specimens were captured in Occitania (south-west of France) and morphologically identified by entomologists, with a total of 530 individuals belonging to 171 species and 19 genera. A customised workflow including distance-tree inferences and a second round of entomologist observations, when necessary, was used for the validation of 348 mini-barcodes covering 148 species. Amongst them, 93 species did not have any 16S reference barcode available before our contribution. This high-quality reference library data are freely available to the scientific community, with the aim of facilitating future large-scale characterisation of wild bee communities in a context of pollinators' decline.

Direct Measurement of the Mutation Rate and Its Evolutionary Consequences in a Critically Endangered Mollusk.

The rate at which mutations arise is a fundamental parameter of biology. Despite progress in measuring germline mutation rates across diverse taxa, such estimates are missing for much of Earth's biodiversity. Here, we present the first estimate of a germline mutation rate from the phylum Mollusca. We sequenced three pedigreed families of the white abalone Haliotis sorenseni, a long-lived, large-bodied, and critically endangered mollusk, and estimated a de novo mutation rate of 8.60 × 10-9 single nucleotide mutations per site per generation. This mutation rate is similar to rates measured in vertebrates with comparable generation times and longevity to abalone, and higher than mutation rates measured in faster-reproducing invertebrates. The spectrum of de novo mutations is also similar to that seen in vertebrate species, although an excess of rare C > A polymorphisms in wild individuals suggests that a modifier allele or environmental exposure may have once increased C > A mutation rates. We use our rate to infer baseline effective population sizes (Ne) across multiple Pacific abalone and find that abalone persisted over most of their evolutionary history as large and stable populations, in contrast to extreme fluctuations over recent history and small census sizes in the present day. We then use our mutation rate to infer the timing and pattern of evolution of the abalone genus Haliotis, which was previously unknown due to few fossil calibrations. Our findings are an important step toward understanding mutation rate evolution and they establish a key parameter for conservation and evolutionary genomics research in mollusks.

Uncovering Diversity within the Glomeromycota: Novel Clades, Family Distributions, and Land Use Sensitivity.

Arbuscular mycorrhizal fungi (AMF, phylum Glomeromycota) are essential to plant community diversity and ecosystem functioning. However, increasing human land use represents a major threat to native AMF globally. Characterizing the loss of AMF diversity remains challenging because many taxa are undescribed, resulting in poor documentation of their biogeography and family-level disturbance sensitivity. We survey sites representing native and human-altered ecosystems across the American continents-in Alaska, Kansas, and Brazil-to shed light on these gaps. Using a recently developed pipeline for phylogenetic placement of eDNA, we find evidence for three putative novel clades within the Glomeromycota, sister to Entrophosporaceae, Glomeraceae, and Archaeosporaceae, with evidence for geographic structuring. We further find that taxa in the Diversisporaceae, Glomeraceae, and Entrophosporaceae relatively high families are overrepresented and more diverse in temperate samples. By contrast, the diversity of taxa that cannot be placed into a family is higher in tropical samples, suggesting that tropical sites harbor relatively high undescribed AMF diversity. Moreover, we find evidence that Entrophosporaceae is more tolerant, while Glomeraceae is more sensitive to disturbance. These results underscore the vast undescribed diversity of AMF while highlighting a way forward to systematically improve our understanding of AMF biogeography and response to human disturbance.

Genes of filter-feeding species as a potential toolkit for monitoring microplastic impacts.

Microplastics (MPs) are ubiquitous in the marine environment and impact organisms at multiple levels. Understanding their actual effects on wild populations is urgently needed. This study develops a toolkit to monitor changes in gene expression induced by MPs in natural environments, focusing on filter-feeding and bioindicator species from diverse ecological and taxonomic groups. Six candidate genes -Caspase, HSP70, HSP90, PK, SOD, and VTG- and nine filter-feeding species -two branchiopods, one copepod, five bivalves and one fish- were selected based on differential expression in response to MPs exposure (mainly the widely used polystyrene and polyethylene polymers) reported in over 30 publications. Some genes are particularly determinant, such as HSP70 and HSP90 (key to managing a wide range of stressors) and SOD (critical for addressing oxidative stress), as they are more directly related to stress. PK is related to carbohydrate metabolism (alterations in energy metabolism); VTG is associated with reproductive problems; Caspase mediates in apoptosis. Each gene in the toolkit plays a role depending on the type of stress assessed, and their combination provides a comprehensive understanding of the impacts of MPs. Differences in gene expressions between species and the exposure thresholds were found. These genes were examined in various scenarios with different types, concentrations, and sizes of MPs, alone or with other stressors. The toolkit offers significant advantages, allowing a comprehensive study of the impact of MPs and focusing on filtering bioindicator species, thus enabling pollution assessment and long-term monitoring. It will outperform traditional methods like tissue counts of MPs where only physical damage is visible, providing a deeper understanding. To our knowledge, this is the first toolkit of its kind.

Diversity and Bioactivities of Ulleung Island-Derived Marine Actinomycetes

Actinomycetes are key producers of bioactive secondary metabolites. In this study, sediment samples were collected using a grab sampler from the coast of Ulleung Island and inoculated on various agar media by stamping to isolate Actinomycetes. As a result, 69 Actinomycete strains were isolated and laboratory-cultured from the sediment samples. The isolates were assigned to the genera Streptomyces (44 strains), Nocardia (6 strains), Rhodococcus (6 strains), Mycobacterium (6 strains), Micromonospora (3 strains), Nocardioides (2 strains), Marmoricola (1 strain), and Oerskovia (1 strain) based on 16S rRNA gene sequence analysis. The antibacterial activity and potential toxicity of the culture broth extracts were tested for all isolates. Extracts from some strains exhibited strong antibacterial activity against Acinetobacter baumannii 04 and methicillin-resistant Staphylococcus aureus. Additionally, some extracts were toxic to brine shrimp. This study confirms that marine Actinomycetes from Ulleung Island encompass unique and diverse taxonomic groups and are a good source of bioactive natural products.

Palisade cell geometry in relation to leaf optical and photosynthetic properties in Viburnum.

The optical properties of lobed palisade mesophyll cells remain poorly understood despite their presence in diverse taxa and the critical role of the palisade layer in leaf-light interactions and carbon assimilation. Using micro-computed tomography, 3D ray tracing simulations, and physiological experiments, we tested the interactions between palisade cell geometry, chloroplast localization, light directional quality, and leaf optical and photosynthetic performance in the model taxon Viburnum. Simulations showed that lobed cells shifted between absorptance- or transmittance-dominated states depending on chloroplast localization, irrespective of light directional quality. In contrast, columnar palisade optics were driven by light directional quality, with absorptance-dominated properties under diffuse light and transmittance-dominated properties under direct light, irrespective of chloroplast localization. Lobed palisade cells in planta were less densely packed yet more productive on a per cell basis than columnar palisade cells, resulting in interspecific conservation of maximum carbon assimilation rate per unit leaf tissue. For the Viburnum species studied, our results indicate a 'many-to-one' mapping of multiple palisade cell forms to a common rate of photosynthetic productivity per unit tissue. This work highlights the dynamic relationship between palisade mesophyll form and function and informs the anatomical basis of plant carbon assimilation.

Less, but More: New Insights From Appendicularians on Chordate Fgf Evolution and the Divergence of Tunicate Lifestyles.

The impact of gene loss on the diversification of taxa and the emergence of evolutionary innovations remains poorly understood. Here, our investigation on the evolution of the Fibroblast Growth Factors (FGFs) in appendicularian tunicates as a case study reveals a scenario of "less, but more" characterized by massive losses of all Fgf gene subfamilies, except for the Fgf9/16/20 and Fgf11/12/13/14, which in turn underwent two bursts of duplications. Through phylogenetic analysis, synteny conservation, and gene and protein structure, we reconstruct the history of appendicularian Fgf genes, highlighting their paracrine and intracellular functions. An exhaustive analysis of developmental Fgf expression in Oikopleura dioica allows us to identify four associated evolutionary patterns characterizing the "less, but more" conceptual framework: conservation of ancestral functions; function shuffling between paralogs linked to gene losses; innovation of new functions after the duplication bursts; and function extinctions linked to gene losses. Our findings allow us to formulate novel hypotheses about the impact of Fgf losses and duplications on the transition from an ancestral ascidian-like biphasic lifestyle to the fully free-living appendicularians. These hypotheses include massive co-options of Fgfs for the development of the oikoblast and the tail fin; recruitment of Fgf11/12/13/14s into the evolution of a new mouth, and their role modulating neuronal excitability; the evolutionary innovation of an anterior tail FGF signaling source upon the loss of retinoic acid signaling; and the potential link between the loss of Fgf7/10/22 and Fgf8/17/18 and the loss of drastic metamorphosis and tail absorption in appendicularians, in contrast to ascidians.

Life expectancy in ants explains variation in helpfulness regardless of phylogenetic relatedness

Abstract Rescue behavior aims to free a relative from danger. Ants are particularly known for such helpfulness and, perhaps not coincidentally, also show the highest level of social organization in the animal kingdom, i.e., eusociality. However, even among social species such as ants, there is a huge variation in rescue proneness and little is understood about the underlying causes of this variation. In this study, we explore the relationship between helpfulness in the form of rescue and life expectancy, focusing on 14 ant species with diverse phylogenetic backgrounds. We posit that species with longer worker life expectancies are more prone to engaging in rescue actions. To test this, we assessed worker lifespan in each species and conducted behavioral tests simulating entrapment scenarios involving a nestmate ensnared by an artificial obstacle. Observed behaviors involved contact with the nestmate, digging around it, pulling at its body parts, and biting the entrapping obstacle. Our findings reveal that species with longer worker life expectancies exhibit higher proneness to rescue endangered nestmates, irrespective of phylogenetic relatedness. Furthermore, we found no trace of a phylogenetic signal in the life expectancies or helpfulness of workers belonging to different species. The results underscore the significance of life expectancy as a key factor influencing the likelihood of rescue behavior in ants. This phenomenon warrants further investigation, given the varied physiologies, life histories, and ecologies observed among species. Nevertheless, the impact of life expectancy on behavioral patterns in social insects suggests that this parameter is likely significant across diverse taxa.

Optimization of swim depth across diverse taxa during horizontal travel

Semiaquatic taxa, including humans, often swim at the air-water interface where they waste energy generating surface waves. For fully marine animals however, theory predicts the most cost-efficient depth-use pattern for migrating, air-breathing species that do not feed in transit is to travel at around 2 to 3 times the depth of their body diameter, to minimize the vertical distance traveled while avoiding wave drag close to the surface. This has rarely been examined, however, due to depth measurement resolution issues at the surface. Here, we present evidence for the use of this strategy in the wild to the nearest centimeter and document the switch to shallow swimming during naturally occurring long-distance migrations. Using high-resolution depth-accelerometry and video data for little penguins (Eudyptula minor) and loggerhead turtles (Caretta caretta), satellite-relayed data for green turtles (Chelonia mydas), and literature data for further sea turtle, penguin, and whale species, we show that near-surface swimming is likely used broadly across nonforaging diving animals to minimize the cost of transport.

Commentary on the Adaptive Significance of Sociality Around Parturition Events, and Conspecific Support of Parturient Females in Some Social Mammals.

In recent decades, it has become apparent that during parturition events in a number of social mammals, social support behaviours from group mates can be directed to parturient females (and their newborn neonates). Such behaviour has been documented in diverse taxa, across non-human primates, Elephantidae, Cetacea, and Chiroptera, living in a range of social group organisations, from matrilineal groups to cooperatively breeding groups and multi-male, multi-female groups. Since sociality, in association with parturition, has been demonstrated to confer several health benefits to human mothers and neonates, here, we also consider the potential adaptive significance of social support behaviours for other, non-human, social mammals. If appropriate social environments reduce a parturient female's dystocia risk and improve her responsiveness to her neonate following a successful birth, then the impacts of the peri-parturient social environment may ultimately have far-reaching impacts on the mother-neonate dyad's fitness. This seems a logical sequela since the health condition of a neonate at birth and the successful establishment of a strong maternal-neonate bond are often the most critical factors influencing mammalian offspring survival to independence. The principles of kin selection and alliance enhancement may serve to explain the fitness benefits to individuals who support group mates during their parturition and thus the selective advantage conferred to those exhibiting such behaviours. Older, multiparous females appear to hold a particularly important role in the assistance they can provide during the parturition of their group mates, given their greater level of experience of these events. Furthermore, a social birth may have an important influence on horizontal information transfer within a group. In particular, in long-lived, cognitively advanced social mammals (e.g., non-human primates, Elephantidae, Cetacea), witnessing birth events, early neonate responses, and maternal care, and engaging in allomaternal care with young neonates may be essential for nulliparous females' normal development. Such events may serve to prepare them for their own parturition and may improve their own parturition-related survivorship and that of their first-born offspring. Thus, it is vital that a better understanding is gained of the importance and salient features of social births in improving the health and survivorship outcomes for both the mother and her offspring in highly social species. The aim of this commentary is to assemble our current understanding of these highly interconnected themes. We suggest in the future, insights gained through observation of non-human social parturition in domestic and non-domestic species, by a wide and highly interdisciplinary range of stakeholders (including zookeepers, wildlife tourism guides, breeders of domestic animals, indigenous people, and ethologists), will be critical for enhancing our understanding of the influence of social environment on this rarely witnessed, yet highly important life event.

The role of acoustic telemetry in assessing fish connectivity within marine seascapes: A global review.

The study of aquatic animal movements is a rapidly growing field of research, with tracking methodology ever developing and refining. Acoustic telemetry is arguably the most popular method used to study the movements of fish. Despite this method being able to elucidate many aspects of movement behavior, including residency, home range, and migration, among others, one aspect that remains challenging is the study and definition of connectivity, particularly within marine seascapes. As such, this review assesses published literature on acoustic telemetry studies, which have specifically assessed some aspect of fish connectivity, and discusses these in terms of study distribution and overall trends, the diversity of taxa and life stage assessed, the role that large-scale acoustic telemetry networks plays in assessing connectivity of marine fishes, how connectivity studies have been used in an applied context, and proposes definitions linked to specific types of connectivity, which will assist future researchers when conceptualizing studies. Further, methods that can be used in conjunction with acoustic telemetry to complement the data are discussed. Given that marine resources and habitats are intricately connected, this review highlights the critical role that acoustic telemetry can play in assessing this link. It is envisaged that our developed framework of connectivity definitions will assist future studies and stakeholders in assessing ecosystem functioning and ultimately contribute to improved conservation and management of marine fish populations and ecosystems.

Comparative analysis of amino acid sequence level in plant GATA transcription factors

Transcription factors (TFs) are essential regulators of gene expression, influencing numerous biological processes such as development, growth, and cellular responses in plants. Among these, GATA TFs are distinguished by their highly conserved DNA-binding domain, characterized by a class IV zinc finger motif (CX2CX18-20CX2C). This study investigates the amino acid sequence patterns of 5,335 GATA TFs across 165 plant species sourced from the PlantTFDB database (http://planttfdb.gao-lab.org/), encompassing diverse taxonomic groups. Through comparative sequence analysis, I identify conserved domains and structural features that enhance the understanding functional roles, evolutionary conservation, and lineage-specific adaptations of GATA TFs. These findings provide valuable insights into the diversification and functional specialization of GATA TFs, with implications for improving stress tolerance and adaptability in crops. This study contributes to the broader knowledge of transcriptional regulation in plant biology.

ITS amplicon sequencing revealed that rare taxa of tea rhizosphere fungi are closely related to the environment and provide feedback on tea tree diseases.

The rhizospheres of plants and soil microorganisms are intricately interconnected. Tea trees are cultivated extensively on the karst plateau of Guizhou Province, China; however, the understanding of the interactions among fungal communities, community taxa, and diseases impacting tea tree in the soil rhizosphere is limited. Our aim is to offer insights for the advancement of modern agriculture in ecologically fragile karst tea gardens, as well as microbiomics concepts for green and sustainable environmental development. This study utilized the internal transcribed spacer high-throughput sequencing technology to explore the symbiotic relationship between rhizosphere fungi and plant disease feedback in multiple tea estates across the Guizhou Plateau. The ecological preferences and environmental thresholds of fungi were investigated via environmental variables. Furthermore, a correlation was established between different taxa and individual soil functions. Research has indicated that tea leaf blight disrupts symbiotic connections among fungal groups. For various taxa, we found that numerous taxa consistently maintained core positions within the community, whereas rare taxa were able to stabilize due to a high proportion of positive effects. Additionally, abundant taxa presented a wider range of environmental feedback, whereas the rare taxon diversity presented a stronger positive association with the soil Z score. This study contributes to our understanding of the importance of rare taxa in plant rhizosphere soil processes. Emphasis should be placed on the role of rare taxa in pest and disease control within green agriculture while also strengthening systematic development and biogeographical research related to rare taxa in this region.IMPORTANCEIn this study, based on internal transcribed spacer high-throughput sequencing, fungal communities in the rhizosphere soil of tea trees and their interactions with the environment in karst areas were reported, and the symbiotic relationships of different fungal taxa and their feedback to the environment were described in detail by using the knowledge of microbial ecology. On this basis, it was found that tea tree diseases affect the symbiotic relationships of fungal taxa. At the same time, we found that rare taxa have stronger cooperative relationships in response to environmental changes and explored their participation in soil processes based on fungal trait sets. This study will provide basic data for the development of modern agriculture in tea gardens and theoretical basis for the sustainable prevention and control of tea tree diseases.

Assessing the potential impacts of floating Offshore Wind Farms on policy-relevant species: A case study in the Gulf of Roses, NW Mediterranean

Our study investigates for the first time how floating Offshore Wind Farms (OWFs) technologies could impact policy-relevant Mediterranean species, focusing on planned OWFs in the Cape Creus/Gulf of Roses (Spain, NW Mediterranean). Using the Generalized Impact Assessment framework, we identified pressure on diverse taxonomic groups. Our species selection prioritized species under European policy (Birds and Habitats Directives) and international/local conventions protecting flora and fauna, as they are vital biodiversity indicators. Our analysis identified 135 policy-relevant species susceptible to OWF-induced stressors, notably marine mammals, seabirds, elasmobranchs, and benthic macroinvertebrates at the highest risk. Among the different stressors, noise and vibration, along with habitat loss, pose the greatest potential impacts. While decarbonizing energy production is crucial for addressing climate change, preserving ocean biodiversity is equally vital. Our study pioneers the assessment of emerging OWFs potential impacts on Mediterranean species, offering valuable insights for decision-makers during OWF planning.