Water and wastewater pollution by acetamiprid and diuron is considered a serious environmental problem. In this study, chitosan (CHS), a naturally occurring bioadsorbent considered ecologically harmless to remove these micropollutants, was developed as a possible carrier to immobilize laccase (Lac) from Trametes trogii. Polyethylene glycol methyl ether (PEGME) was chosen for blending CHS, so a hybrid biocatalyst-based Lac/CHS-PEGME membrane was prepared. The prepared CHS-PEGME and Lac/CHS-PEGME membranes were characterized by Fourier-transformed-infrared (FTIR) spectroscopy, scanning-electron-microscopy (SEM), and X-ray-diffraction (XRD). Pesticide degradation tests with Lac/CHS-PEGME were performed at different contact times and initial concentrations. Acetamiprid degradation was most effective (84 %) at the 12th hour, at an initial concentration of 0.1 mg/L, while diuron degradation was most effective (65 %) at an initial concentration of 6 mg/L and a contact time of 16th hour. Under optimum conditions, the reusability of Lac/CHS-PEGME was found to be 8 cycles for acetamiprid and 5 cycles for diuron. From these results, it is understood that acetamiprid is degraded more quickly and effectively than diuron. Adsorption process data were well fitted to the Langmuir isotherm model and the pseudo-first-order kinetic model. These findings showed that using Lac/CHS-PEGME was a practical and environmentally friendly method for acetamiprid and diuron degradation.