The diurnal cycle of convection plays an important role in clouds and water vapour distribution across the global tropics. In this study, we utilize integrated moisture derived from the global navigation satellite system (GNSS), satellite precipitation estimates from TRMM and merged infrared dataset to investigate links between variability in tropospheric moisture, clouds development and precipitation at a diurnal time scale. Over 16 years of observations were analysed to estimate the diurnal cycles for 42 sites that span across the global tropics and assess the GNSS precipitable water vapour suitability in tropical moisture variability studies. Results show that the GNSS technique can be successfully used for studies related to the high-resolution temporal variability of integrated atmospheric moisture and, thus, in the analysis of multi-scale interactions and clouds development. For most of the analysed cases, GNSS integrated humidity shows one daily maximum (regardless of the season), which is related to the afternoon precipitation and cloud top temperature daily minimum. Although diurnal integrated moisture variations typically do not exceed 2 mm (usually below 5% of the daily mean value), in favourable large-scale conditions such relatively small changes can lead to the development of convective clouds and can affect precipitation distribution. Thus, GNSS technology allows reliable monitoring of tropospheric moisture variability at a diurnal time scale, which can be leveraged for improved monitoring and prediction of high-impact weather.