AbstractFor the first time, characteristics of the geographical and seasonal distribution of the quasi‐diurnal lunar O1 tide were derived from a time series of ionospheric total electron content (TEC) maps provided by International Global Navigation Satellite System Service (IGS). The data analysis is focused on solar minimum in 2008 and 2009 where disturbing influences of geomagnetic and solar activity were minimal. We found that the magnitude of the O1 tide is as strong as the “dominant” semidiurnal lunar M2 tide. Relative amplitudes of 10% and larger are observed in some regions for the O1 component in TEC. The O1 component is particularly strong in northern hemispheric winter over the west coast of South America. There, two maxima occur which are northward and southward of the magnetic equator in the Equatorial Ionization Anomaly (EIA) crest regions. Following Yamazaki et al. (2017, https://doi.org/10.1002/2017ja024601), it might be assumed that a longitudinal anomaly of ionospheric conductivities in the Peruvian sector leads to a stronger modulation of the equatorial electrojet by the lunar tides. Electrodynamic lifting of plasma and transport to the EIA crests may explain the variations of the O1 component in TEC. Contrary to many studies, we find the O1 component (period 25.82 hr) more important than the M1 component (period 24.84 hr, a lunar day). We show that the geographical distribution of the O1 component is totally different from that of the M1 component which is smaller. The seasonal variation of O1 shows maximal amplitudes in northern hemispheric winter and minimal amplitudes in southern hemispheric winter.