Shield tunnelling in sensitive soil causes disturbance to the surrounding soil, damages the soil structure, and results in soil strength reduction. It has a significant impact on the strength and deformation of the soil during shield tunnelling, and further affects the long-term post-construction settlement of the tunnel structure. This paper firstly obtained the soil disturbance and its distribution especially under the tunnel by the in-situ measurement, and the effect of tunneling advancing speed on soil disturbance degree was analyzed. In this paper, in situ tests were performed on an earth pressure balanced (EPB) shield tunnelling site of a subway in deep soft soil layers in Ningbo. Before and after the shield tunnelling, the cone penetration tests (CPTs) were carried out along the axis and profile of the tunnel, and the cross-hole shear wave velocity tests were conducted. Displacement of the surrounding soil and excess pore water pressure (EPWP) were also monitored during shield tunnelling. The results showed that after shield tunnelling the cone penetration resistance (CPR) and shear wave velocity of the surrounding soil decreased remarkably, further confirming that shield tunnelling caused disturbance to the sensitive soft soil. The soil disturbance degree (SDD) is defined based on the CPR, and the soil disturbance boundary caused by shield tunnelling is determined based on distributions of SDD and EPWP. Horizontally, the disturbance boundary of the surrounding soil is located about 0.8D (D represents the tunnel diameter) outside the tunnel, and vertically, the bottom and top of the disturbance boundary are located about 1.29D below and 0.58D above the tunnel. The maximum SDD at the bottom and top of the tunnel reached 80% and 90% respectively, and the average SDD of the disturbance range below the tunnel is about 60%. The testing results indicated that a higher advancing speed causes a greater disturbance to the surrounding soil, and all the analysis results provide helpful guidance for optimizing the EPB shield tunnelling and reducing soil disturbance.