Disturbed Sites Research Articles

A graphical non-parametric hydrologic alteration test using flow duration curves

ABSTRACT Utilizing annual flow duration curves (FDCs), the modified Mood’s median test (MMMT) was applied to quantiles of the median annual FDCs at 122 disturbed sites in the conterminous US. A simple graphical tool based on the MMMT was combined with a false discovery rate multiple comparison procedure to illustrate the field significance of hydrologic alteration over a wide range of streamflow conditions. Hydrologic disturbance was mostly caused by dams, although other forms of alteration were explored. In addition, 249 minimally regulated Hydro-Climatic Data Network (HCDN) sites were investigated to assess regional climatic drivers of hydrologic alteration. Results indicate that dams can have varying effects on the hydrologic regime, but generally impact low flows more often than high flows in the US. At HCDN sites, we observe significant changes to hydrologic regimes in many regions, especially to low and median flows in the upper midwestern and eastern US.

A taxonomic revision of Cenchrus L. (Poaceae) in Thailand, with lectotypification of Pennisetummacrostachyum Benth.

A revision of the genus Cenchrus (Poaceae) in Thailand is reported. Seven species, i.e. C.brownii, C.ciliaris, C.clandestinus, C.echinatus, C.pedicellatus, C.purpureus and C.setosus are described in this taxonomic treatment. This genus is an exotic species and distributed throughout the floristic regions. All the species have become naturalised to Thailand as a weed and found growing in wastelands, open areas by the roadside, disturbed sites, the edge of rice fields and the edge of deciduous and evergreen forests at elevations between 0 and 2,650 m alt. Pennisetummacrostachyum, a synonym of Cenchruspurpureus is lectotypified. Detailed morphological descriptions, illustrations and a key to the species are presented, along with information on distributions, habitats, ecology, phenology, vernacular names and specimens examined.

Microclimate and seed bank under Pithecellobium keyense (Fabaceae) in a coastal scrubland

AbstractMicroclimatic conditions affect seeds' survival, dormancy, and germination, which are determinants in vegetation succession and recovery. The semiarid coastal scrublands of Yucatan, Mexico are very important because of the environmental services they provide thus, studies are needed in disturbed sites in relation to seed viability and microclimate. In this study, we characterized the microclimate and seed bank under Pithecellobium keyense shrubs, during the rainy and dry seasons. We collected soil samples and measured microenvironmental variables under adult P. keyense at three different positions under the canopy of every studied individual (“open” or completely sunny, “half‐shaded,” placed at the border of the canopy, and “fully shaded” placed under the canopy, next to the stem of the shrub). Photosynthetic photon flux density (PPFD), minimum night‐time temperature, and maximum daytime temperature behaved differently among positions under the canopy both in rainy and dry seasons. During the rainy season, seed viability was no different among microenvironments, while in the dry, the highest was found in the fully shaded and the lowest in the open position. Microclimatic variables under the canopy of P. keyense had an effect on the probability of seed viability in Chuburná; and this effect was stronger in the dry season.Abstract in Spanish is available with online material.

Аккумуляционные способности мохообразных нарушенных торфяников (на примере карбонового полигона в Калининградской области)

The paper provides original data on accumulation capabilities of bryophytes typical of peatland ecosystems with different degree of anthropogenic transformation occurring in the Kaliningrad Region of Russia. A key study area was the Vittgirrensky Peatland, abandoned after milled peat extraction in 1990s and designated as the Rossyanka Carbon Measurement Supersite in 2021. The accumulation of micro- and macroelements: Ca, Mn, Fe, Ni, Zn, Br, Rb, and Sr – was identified by means of X-ray fluorescence spectroscopy in 13 bryophyte species (Aulacomnium palustre, Campylopus introflexus, Polytrichum commune, P. strictum, Sphagnum capillifolium, S. centrale, S. cuspidatum, S. fuscum, S. magellanicum, S. riparium, S. squarrosum, S. teres) focusing on comparison with the reference species Pleurozium schreberi. The records of average element concentrations from the Vittgirrensky Peatland are shown to be distinctly lower than the regional background level. The accumulation of Mn, Ni, Br, Rb, and Sr varies significantly among disturbed and natural sites: the concentrations are comparable for Fe and Zn, while cut-over peatlands showed the level of Mn several times less than in undisturbed peat bogs. Aulacomnium palustre can be recommended for passive biomonitoring purposes on bog ecosystems as having most similar accumulation capability to the reference species Pleurozium schreberi that is widely recognized as indicator of atmospheric air pollution.

Species Composition and Diversity of Plants along Human-Induced Disturbances in Tropical Moist Sal Forests of Eastern Ghats, India

Plant biodiversity data are prerequisites for the sustainable management of a forest. We used quantitative ecological tools to determine the species composition, diversity (richness and evenness), population structure, distribution patterns, and regeneration status of trees in a Tropical Moist Sal Forest of Eastern Ghats, India. For this purpose, a field inventory was conducted during 2020–2022 in sixteen 1.0 ha forest stands along a human-induced disturbance gradient. A total of 161 species (61 trees, 40 shrubs, 60 herbs) belonging to 77 families and 143 genera were recorded in the 16.0 ha of forest area. The results revealed a significant (p < 0.01) decrease in the tree and shrub species density, basal area, species richness, and diversity along the gradient of disturbance. However, in the case of herbs, the Shannon–Weiner diversity index increased significantly (p < 0.01) with increasing disturbance levels. Irrespective of forest types and disturbance levels, the tree diameter class (10–30 cm) accounted for the highest stem density in the forest. A mixed trend was found in the case of the basal area; the >41 cm diameter class constituted the highest basal area in the Pure Sal Forest while the 10–30 cm class was in the Moist Deciduous Forest without Sal. Tree species richness was found higher in lower diameter classes. Disturbances impacted the distribution pattern of trees; in the Pure Sal Forest, the contagious distribution of trees were 61.54%, 40%, and 12.5% in undisturbed, low-disturbed, and moderately disturbed sites, respectively. The percent of trees showing random and regular distribution increased with the increased level of disturbance in all forest types. The number of tree species having good regeneration decreased with the increased disturbance intensity in all forest types. Frequent grazing, repeated forest fires, and poor soil seed banks at the Moderately Disturbed site were the main reasons for the poor/no regeneration of Pterocarpus marsupium, Adina cordifolia, Terminalia bellerica, and some other economical species. Significant changes in structural attributes of the tree community revealed the impact of human-induced disturbances in the Moist Sal Forests of Eastern Ghats. The disturbance mosaics promoted the growth of many invasive weed species and lianas, depleting the number of valuable species in the forest. This study suggests the adaption of sustainable biodiversity conservation approaches through the active participation of the tribal so that the remnants the Moist Sal Forests of Eastern Ghats can be controlled to prevent further degradation.

Reproductive Responses to Increased Shoot Density and Global Change Drivers in a Widespread Clonal Wetland Species, Schoenoplectus americanus

The expansion of many wetland species is a function of both clonal propagation and sexual reproduction. The production of ramets through clonal propagation enables plants to move and occupy space near parent ramets, while seeds produced by sexual reproduction enable species to disperse and colonize open or disturbed sites both near and far from parents. The balance between clonal propagation and sexual reproduction is known to vary with plant density but few studies have focused on reproductive allocation with density changes in response to global climate change. Schoenoplectus americanus is a widespread clonal wetland species in North America and a dominant species in Chesapeake Bay brackish tidal wetlands. Long-term experiments on responses of S. americanus to global change provided the opportunity to compare the two modes of propagation under different treatments. Seed production increased with increasing shoot density, supporting the hypothesis that factors causing increased clonal reproduction (e.g., higher shoot density) stimulate sexual reproduction and dispersal of genets. The increase in allocation to sexual reproduction was mainly the result of an increase in the number of ramets that flowered and not an increase in the number of seeds per reproductive shoot, or the ratio between the number of flowers produced per inflorescence and the number of flowers that developed into seeds. Seed production increased in response to increasing temperatures and decreased or did not change in response to increased CO2 or nitrogen. Results from this comparative study demonstrate that plant responses to global change treatments affect resource allocation and can alter the ability of species to produce seeds.

Species diversity and detection of pathogens in phlebotomine sand flies collected from forest management areas of Quintana Roo, Mexico.

Sand flies have expanded their areas of distribution, thereby increasing the risk of pathogen transmission in non-endemic areas. To establish efficient prevention and control strategies for the transmission of vector-borne pathogens, it is important to understand seasonal dynamics of their vectors. In Mexico, there are several areas where the contact between sand flies, hosts and reservoirs favours the transmission of the pathogen. We compared sand fly communities in a forest management area and a conserved area in Noh-Bec, Quintana Roo, Mexico. The analysis included species diversity, activity peaks and molecular detection of pathogens. Sand flies were collected from November to December 2021 and April to May 2022, during 84 night-traps. The conserved area showed higher numbers and greater species heterogeneity of sand flies as compared with the other sites. The β-diversity analysis revealed that sites disturbed by logging (S1, S2, S3) had greater similarity (90%) in their sand fly species composition than a conserved area (S4) (similarity = 36%). Although none of the specimens were infected with Leishmania, we detected Wolbachia (19.4%) in all four sites, as well as Bartonella (3.25%) only in the disturbed sites. Further studies on the dynamics of sand fly populations and their association with pathogens are necessary.

Highlighting Hemodynamic Risks for Bioresorbable Stents in Coronary Arteries

A three-dimensional, transient computational fluid dynamics analysis was conducted on an idealised geometry of a coronary artery fitted with representative geometries of an Absorb bioresorbable vascular scaffold (BVS) or a Xience drug-eluting stent (DES) in order to identify and compare areas of disturbed flow and potential risk sites. A non-Newtonian viscosity model was used with a transient velocity boundary condition programmed with user-defined functions. At-risk areas were quantified in terms of several parameters linked to restenosis: wall shear stress, time-averaged wall shear stress, oscillatory shear index, particle residence time, and shear rate. Results indicated that 71% of the BVS stented surface area had time-averaged wall shear stress values under 0.4 Pa compared to 45% of the DES area. Additionally, high particle residence times were present in 23% and 8% of the BVS and DES areas, respectively, with risk areas identified as being more prominent in close proximity to crowns and link struts. These results suggest an increased risk for thrombosis and neointimal hyperplasia for the BVS compared to the DES, which is in agreement with the outcomes of clinical trials. It is intended that the results of this study may be used as a pre-clinical tool to aid in the design of bioresorbable coronary stents.

Roles of RIPK1 as a stress sentinel coordinating cell survival and immunogenic cell death.

Cell death and inflammation are closely linked arms of the innate immune response to combat infection and tissue malfunction. Recent advancements in our understanding of the intricate signals originating from dying cells have revealed that cell death serves as more than just an end point. It facilitates the exchange of information between the dying cell and cells of the tissue microenvironment, particularly immune cells, alerting and recruiting them to the site of disturbance. Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) is emerging as a critical stress sentinel that functions as a molecular switch, governing cellular survival, inflammatory responses and immunogenic cell death signalling. Its tight regulation involves multiple layers of post-translational modifications. In this Review, we discuss the molecular mechanisms that regulate RIPK1 to maintain homeostasis and cellular survival in healthy cells, yet drive cell death in a context-dependent manner. We address how RIPK1 mutations or aberrant regulation is associated with inflammatory and autoimmune disorders and cancer. Moreover, we tease apart what is known about catalytic and non-catalytic roles of RIPK1 and discuss the successes and pitfalls of current strategies that aim to target RIPK1 in the clinic.

The effects of herbivory, land-use and climate change on seedling establishment in southern Africa

In intact ecosystems, factors such as herbivory and light and water availability affect seedling establishment. Land-use change and climate change are now affecting forests globally, influencing seedling establishment directly via changes in light availability and precipitation and indirectly by affecting herbivory. The present study evaluated the ecological complexity of seedling establishment in response to interactions between these factors. The growth and survival of a pioneer species and two later successional species were measured in a factorial experiment testing the effects of land-use change, water availability, and herbivory. The study was conducted on Mt. Gorongosa in central Mozambique, a protected area that is experiencing high levels of land-use change and is in need of forest restoration. The pioneer species, Erythrina lysistemon, gained the most height in undisturbed, shaded environments where it invested resources in accessing light. Erythrina lysistemon grew the most in diameter in open areas. Khaya anthotheca, a later successional species, gained more biomass in disturbed, high light areas. The growth of Millettia stuhlmannii, another later successional species, was not affected by light. Drought conditions reduced survival of the later successional species, while flooding limited the survival of the pioneer species. Herbivory increased in disturbed areas and was negatively correlated with E. lysistemon root biomass. In summary, this study indicates land-use change and climate change may alter seedling establishment and community composition. Restoration efforts should focus on increasing seedling survival because although survival is limited in disturbed sites, seedlings that do survive grow successfully.

Striving for consistent bioassessment across diverse landscapes: Using land use matters for classifying reference and disturbed sites for index development

Consistency in ecological assessments is challenging across large diverse landscapes because natural geological, climatic, and hydrological factors vary greatly. As a result, large landscapes are often subdivided into ecoregions and assessments are based on ecoregion specific indices. In the present study, we developed and compared multimetric indices (MMIs) using benthic diatom data from the 2008–2009 dataset from the United States (US) National Rivers and Streams Assessment. Nationwide and separate ecoregion specific MMIs were developed with reference, moderately disturbed, and highly disturbed sites selected using criteria based on physicochemical condition of the habitat or based on watershed land use (% agriculture and % urban). Metrics were adjusted to account for variation in natural conditions when needed. We found only land use criteria selected reference sites with consistently low median % watershed disturbance (%WD) and large differences in %WD between reference and highly disturbed sites. <38 % of sites were identified as reference or highly disturbed by both physicochemical and land use criteria. All MMIs displayed substantial discrimination ability between reference and highly disturbed sites. At the national scale, MMIs based on land use outperformed MMIs based on physicochemical conditions for all performance attributes tested. When national scale MMIs were applied to ecoregions, MMIs based on land use were again better than MMIs based on physicochemical conditions for most performance attributes and even had better or comparable performance to the land use MMIs developed separately for each ecoregion. Our findings show that developing MMIs using land use criteria and adjusting metrics for natural variation could improve assessment consistency without losing MMI performance across large, diverse landscapes as in the US National Rivers and Streams Assessment.

Spatial proteomics landscape and immune signature analysis of renal sample of lupus nephritis based on laser-captured microsection

ObjectiveWe aimed to reveal a spatial proteomic and immune signature of kidney function regions in lupus nephritis (LN).Material and methodsThe laser capture microdissection (LCM) was used to isolate the glomerulus, tubules, and interstitial of the kidney from paraffin samples. The data-independent acquisition (DIA) method was used to collect proteomics data. The bioinformatic analysis was performed.ResultsA total of 49,658 peptides and 4056 proteins were quantitated. Our results first showed that a high proportion of activated NK cells, naive B cells, and neutrophils in the glomerulus, activated NK cells in interstitial, and resting NK cells were accumulated in tubules in LN. The immune-related function analysis of differential expression proteins in different regions indicated that the glomerulus and interstitial were major sites of immune disturbance and regulation connected with immune response activation. Furthermore, we identified 7, 8, and 9 hub genes in LN’s glomerulus, renal interstitial, and tubules. These hub genes were significantly correlated with the infiltration of immune cell subsets. We screened out ALB, CTSB, LCN2, A2M, CDC42, VIM, LTF, and CD14, which show higher performance as candidate biomarkers after correlation analysis with clinical indexes. The function within three regions of the kidney was analyzed. The differential expression proteins (DEGs) between interstitial and glomerulus were significantly enriched in the immune-related biological processes, and myeloid leukocyte-mediated immunity and cellular response to hormone stimulus. The DEGs between tubules and glomerulus were significantly enriched in cell activation and leukocyte-mediated immunity. While the DEGs between tubules and interstitial were enriched in response to lipid, antigen processing, and presentation of peptide antigen response to oxygen-containing compound, the results indicated a different function within kidney regions.ConclusionsCollectively, we revealed spatial proteomics and immune signature of LN kidney regions by combined using LCM and DIA.

Pioneer Arabidopsis thaliana spans the succession gradient revealing a diverse root-associated microbiome

BackgroundSoil microbiomes are increasingly acknowledged to affect plant functioning. Research in molecular model species Arabidopsis thaliana has given detailed insights of such plant-microbiome interactions. However, the circumstances under which natural A. thaliana plants have been studied so far might represent only a subset of A. thaliana’s full ecological context and potential biotic diversity of its root-associated microbiome.ResultsWe collected A. thaliana root-associated soils from a secondary succession gradient covering 40 years of land abandonment. All field sites were situated on the same parent soil material and in the same climatic region. By sequencing the bacterial and fungal communities and soil abiotic analysis we discovered differences in both the biotic and abiotic composition of the root-associated soil of A. thaliana and these differences are in accordance with the successional class of the field sites. As the studied sites all have been under (former) agricultural use, and a climatic cline is absent, we were able to reveal a more complete variety of ecological contexts A. thaliana can appear and sustain in.ConclusionsOur findings lead to the conclusion that although A. thaliana is considered a pioneer plant species and previously almost exclusively studied in early succession and disturbed sites, plants can successfully establish in soils which have experienced years of ecological development. Thereby, A. thaliana can be exposed to a much wider variation in soil ecological context than is currently presumed. This knowledge opens up new opportunities to enhance our understanding of causal plant-microbiome interactions as A. thaliana cannot only grow in contrasting soil biotic and abiotic conditions along a latitudinal gradient, but also when those conditions vary along a secondary succession gradient. Future research could give insights in important plant factors to grow in more ecologically complex later-secondary succession soils, which is an impending direction of our current agricultural systems.

Habitat scale and seasonality influence macroinvertebrate functional feeding groups in a tropical Kenyan montane stream

Habitat quality and diversity strongly influence the composition of macroinvertebrate communities in lotic ecosystems. We evaluated the functional organization of macroinvertebrates in response to changes in habitat type and seasonality in the Afromontane Moiben River in western Kenya. In-stream substrate characteristics were evaluated at the reach, channel unit/habitat and microhabitat scales at nine sites in different land-use areas. Water and habitat quality were assessed during the dry (January–March) and wet (April–June) seasons. A total of 81 macroinvertebrate samples were collected every month from three channel units (pools, riffles and runs) defined by different substrate types and classified into five functional feeding groups (FFGs): collector-filterers, collector-gatherers, predators, scrapers and shredders. Numerical abundance differed significantly between FFGs, with gatherers > filterers > predators > scrapers > shredders. Coarse substrate (bedrock, boulders and cobbles) in riffles had the highest richness and abundance of filterers and scrapers while sand, mud and detritus in pools recorded the lowest richness and abundance. Shredders and scrapers occurred predominantly in upstream sites with minimal human disturbance, while gatherers were most abundant at disturbed sites. The greatest variability in FFGs was at the smallest microhabitat scale, and not at the reach scale. This study shows that attention should be given to both reach-scale and in-stream disturbances when assessing the ecological condition of streams and rivers.

Community specialisation in vegetation succession on central‐European disturbed sites

AbstractQuestionsConservation and restoration efforts can greatly benefit from understanding how plant community specialisation changes after disturbance. This paper addresses general successional patterns concerning the following questions: How does community specialisation change during succession? What are the general patterns in the occurrence of generalists and specialists? What are the general ecological strategies of generalists and specialists? Are threatened species better represented among specialists, as generally expected?LocationCzech Republic, central Europe.MethodsWe assessed trends in plant community specialisation as well as the participation of generalists and specialists in 12 types of successional series based on a large data set of dated plots of spontaneously established vegetation at various disturbed sites. We classified species into generalists and specialists using a co‐occurrence specialisation metric, determined overall community specialisation at the plot level, and assessed trends in response to successional age for each of the seres using regression analyses, community‐weighted means and the fourth‐corner approach. We also examined the differences between generalists and specialists during succession by comparing their ecological strategies (competitive, stress‐tolerant and ruderal) and conservation status.ResultsCommunity specialisation generally increased during succession, mainly due to a decline in generalists and an increase in typical species of particular habitats such as grasslands, woodlands and wetlands. Despite the general increase in community specialisation, specialists peaked in early and old successional stages and were always less abundant than generalists. During succession, generalists were more stable in their ecological strategies and were predominantly competitive. Specialists varied more, being mostly competitive stress tolerators but also ruderals in early successional stages. Specialists were proportionally more threatened than generalists.ConclusionsGeneralists and specialists differ in their successional patterns, ecological strategies and conservation status. Assessing the spatio‐temporal changes in community specialisation helps to evaluate and track successional trends, and to determine the conservation and restoration status of degraded areas.

Recovery of vegetation on former alpine roads: how long does it take?

Alpine areas worldwide are under heavy land‐use pressure and degradation. Active restoration treatments can contribute to accelerating recovery of degraded areas. However, monitoring data are needed to understand the contribution of restoration treatments to long‐term management and to predict time to recovery (TR). In this study, we used monitoring data on removed roads in an alpine area in Norway to investigate TR of three vegetation‐based indicators. Four restoration treatments were tested: 1) removal of added gravel down to original terrain surface, and stirring of topsoil; 2) adding fertilizer to the stirred topsoil; 3) adding seeds to the fertilized topsoil; and 4) no removal of added gravel, but stirring of top layer (gravel and soil). The restoration of roads took place in 2002, and monitoring of permanent plots was carried out in 2004, 2009, 2014, and 2019. Reference plots in intact vegetation next to removed roads were monitored in 2014 and 2019. We used species composition and species richness of vascular plants as well as total vegetation cover as indicators of restoration outcome and investigated predicted TR for these indicators under different restoration treatments. Species composition changed significantly with time since restoration in all treatments, approaching that of the reference vegetation. The recovery of species composition was slowest in fertilized and seeded plots, where estimated TR was 2–3 times longer (&gt; 45 years) than in the other treatments (&lt; 20 years). Species richness of vascular plants was restored quickly (&lt; 5 years) within all restoration treatments, whereas recovery of vegetation cover varied more (20–30 years). Our study confirms that vegetation recovery in alpine environments is a long‐term process, but that adding seeds and nutrients is unnecessary for, and even inhibits, the recovery of narrow, disturbed sites such as former roads.

Warming, drought, and disturbances lead to shifts in functional composition: A millennial-scale analysis for Amazonian and Andean sites.

Tropical forests are changing in composition and productivity, probably in response to changes in climate and disturbances. The responses to these multiple environmental drivers, and the mechanisms underlying the changes, remain largely unknown. Here, we use a functional trait approach on timescales of 10,000 years to assess how climate and disturbances influence the community-mean adult height, leaf area, seed mass, and wood density for eight lowland and highland forest landscapes. To do so, we combine data of eight fossil pollen records with functional traits and proxies for climate (temperature, precipitation, and El Niño frequency) and disturbances (fire and general disturbances). We found that temperature and disturbances were the most important drivers of changes in functional composition. Increased water availability (high precipitation and low El Niño frequency) generally led to more acquisitive trait composition (large leaves and soft wood). In lowland forests, warmer climates decreased community-mean height probably because of increased water stress, whereas in highland forests warmer climates increased height probably because of upslope migration of taller species. Disturbance increased the abundance of acquisitive, disturbance-adapted taxa with small seeds for quick colonization of disturbed sites, large leaves for light capture, and soft wood to attain fast height growth. Fire had weak effects on lowland forests but led to more stress-adapted taxa that are tall with fast life cycles and small seeds that can quickly colonize burned sites. Site-specific analyses were largely in line with cross-site analyses, except for varying site-level effects of El Niño frequency and fire activity, possibly because regional patterns in El Niño are not a good predictor of local changes, and charcoal abundances do not reflect fire intensity or severity. With future global changes, tropical Amazonian and Andean forests may transition toward shorter, drought- and disturbance-adapted forests in the lowlands but taller forests in the highlands.

Is the protection of habitat directive Eleocharis carniolica in its northern limits really needed? – A life strategy based investigation

Eleocharis carniolica is included in the EU Habitat Directive and legally protected in many European countries. It is also considered as one of the most threatened plants in Poland. It typically occurs in muddy habitats, wet meadows, lake or pond shores or temporary puddles, but in Poland this species usually occurs at disturbed anthropogenic sites such as road verges or drainage ditches. Despite being the focus of a number of ecological and conservation studies, E. carniolica still does not have a fully recognized ecology and life-strategy, particularly with regard to breaking seed dormancy and germination. To find out the population performance and species propagation potential in Poland, we have conducted experiments aimed at assessing the seed germination conditions and efficiency. The main goal was to discover the crucial factors that stimulate germination. The results of the experiments suggest that light is a pivotal factor for triggering germination of E. carniolica seeds. Moreover, E. carniolica’s seed bank consisted of about 80 % dormant seeds. The only effective method for dormancy breaking was warm stratification (placing the seeds at high temperature on a moist substrate) with application of gibberellic acid (GA3), and even a few days of warm stratification resulted in effectively overcoming dormancy. Eleocharis carniolica's germination features are typical for plants adapted to frequent disturbances with a short life expectancy and large reproductive effort. Our results strongly suggest that E. carniolica has a ruderal life strategy at its northern distribution limits and probably can be considered as an alien species in Poland.

Systemwide effects of ER-intracellular membrane contact site disturbance in primary endothelial cells

Membrane contact sites (MCS) make up a crucial route of inter-organelle non-vesicular transport within the cell. Multiple proteins are involved in this process, which includes the ER-resident proteins vesicle associated membrane protein associated protein A and -B (VAPA/B) that form MCS between the ER and other membrane compartments. Currently most functional data on VAP depleted phenotypes have shown alterations in lipid homeostasis, induction of ER stress, dysfunction of UPR and autophagy, as well as neurodegeneration. Literature on concurrent silencing of VAPA/B is still sparse; therefore, we investigated how it affects the macromolecule pools of primary endothelial cells. Our transcriptomics results showed significant upregulation in genes related to inflammation, ER and Golgi dysfunction, ER stress, cell adhesion, as well as Coat Protein Complex-I and -II (COP-I, COP-II) vesicle transport. Genes related to cellular division were downregulated, as well as key genes of lipid and sterol biosynthesis. Lipidomics analyses revealed reductions in cholesteryl esters, very long chain highly unsaturated and saturated lipids, whereas increases in free cholesterol and relatively short chain unsaturated lipids were evident. Furthermore, the knockdown resulted in an inhibition of angiogenesis in vitro. We speculate that ER MCS depletion has led to multifaceted outcomes, which include elevated ER free cholesterol content and ER stress, alterations in lipid metabolism, ER-Golgi function and vesicle transport, which have led to a reduction in angiogenesis. The silencing also induced an inflammatory response, consistent with upregulation of markers of early atherogenesis. To conclude, ER MCS mediated by VAPA/B play a crucial role in maintaining cholesterol traffic and sustain normal endothelial functions.

Effect of Canal Bank Engineering Disturbance on Plant Communities: Analysis of Taxonomic and Functional Beta Diversity

We aimed to determine how patterns of functional and taxonomic dissimilarities and their components differ between disturbed and undisturbed plant communities. Taxonomic (species) and functional (trait) diversity are key aspects of biodiversity, and their respective dissimilarities are important in diversity scaling and for informing conservation. We utilized a pseudo-experimental setting, the Basingstoke Canal, UK, where sections of canal bank have been repaired over a four-year period and are interspersed with sections left undisturbed. We collected plant community data, computed functional beta diversity and taxonomic beta diversity and partitioned them into species loss and replacement components. We compared disturbed and undisturbed plots with respect to these dissimilarity measures, the time since disturbance, invasive species, plant life-forms and environmental dissimilarity. We found high levels of taxonomic (85–90%) and functional (70–76%) dissimilarities between disturbed and undisturbed sites, primarily driven by turnover. The total dissimilarity was lower for functional dissimilarity than taxonomic dissimilarity. Disturbed sites had greater between-site taxonomic and functional dissimilarities and lower plant abundances than undisturbed sites, driven by both turnover and nestedness components. The disturbed site functional diversity diverged strongly from null expectations. We found no significant effects of time since disturbance, environmental variables or invasive species, possibly indicating the dominance of stochastic, local-scale processes. However, disturbed sites had lower levels of phanerophyte richness and higher levels of therophyte richness. Our results indicate that small-scale disturbances may increase taxonomic and functional between-community dissimilarities in anthropogenic habitats without increasing invasive species, lending support to local-scale conservation that enhances habitat heterogeneity to promote taxonomic diversity and its corresponding biotic functions.