Range Of Species Research Articles

Combining distribution modelling and phylogeography to understand present, past and future of an endangered spider

BackgroundUnderstanding how endangered species respond to climatic changes is fundamental for their conservation. Due to its restricted geographic range, its sensitivity to the ongoing global warming and its continuing decline, the Southwestern-Alpine endemic wolf spider Vesubia jugorum is currently classified as Endangered in the IUCN Red List. Here, we combined species distribution modelling (SDM) and phylogeographic inference to describe the present, the past and the future of this species in light of the mtDNA genetic structure of extant populations.ResultsPhylogenetic and network analyses show a high level of genetic differentiation and a strong genetic structure of the populations, likely explicable by a long history of isolation and survival in separate refugia. The SDM projection into past climatic conditions supports these results by showing a smaller distribution range compared to present, mostly restricted to the Maritime and Ligurian Alps, which possibly served as main refugium. Future forecast shows a significant shift in the bioclimatic range towards higher altitudes and latitudes, with a drastic decrease of habitat suitability in the central and south-eastern parts of the range, with consequent general loss of haplotype diversity.ConclusionSDM and phylogeographic inference support the hypothesis that the current distribution and the genetic structure of the extant populations mirror the survival in situ of Vesubia jugorum across repeated glacial and interglacial phases, in line with the ‘long-term stability hypothesis’. Future predictions show a significant shift in the bioclimatic range that V. jugorum will be likely unable to track, with profound impact on its long-term survival and its genetic diversity. Our considerations have implication for conservation genetics, highlighting the pivotal role of the transboundary protected areas of the SW-Alps in promoting conservation efforts for this species.

Unearthing Optimal Symbiotic Rhizobia Partners from the Main Production Area of Phaseolus vulgaris in Yunnan.

Phaseolus vulgaris is a globally important legume cash crop, which can carry out symbiotic nitrogen fixation with rhizobia. The presence of suitable rhizobia in cultivating soils is crucial for legume cropping, especially in areas beyond the plant-host native range, where soils may lack efficient symbiotic partners. We analyzed the distribution patterns and traits of native rhizobia associated with P. vulgaris in soils of Yunnan, where the common bean experienced a recent expansion. A total of 608 rhizobial isolates were tracked from soils of fifteen sampling sites using two local varieties of P. vulgaris. The isolates were discriminated into 43 genotypes as defined by IGS PCR-RFLP. Multiple locus sequence analysis based on recA, atpD and rpoB of representative strains placed them into 11 rhizobial species of Rhizobium involving Rhizobium sophorae, Rhizobium acidisoli, Rhizobium ecuadorense, Rhizobium hidalgonense, Rhizobium vallis, Rhizobium sophoriradicis, Rhizobium croatiense, Rhizobium anhuiense, Rhizobium phaseoli, Rhizobium chutanense and Rhizobium etli, and five unknown Rhizobium species; Rhizobium genosp. I~V. R. phaseoli and R. anhuiense were the dominant species (28.0% and 28.8%) most widely distributed, followed by R. croatiense (14.8%). The other rhizobial species were less numerous or site-specific. Phylogenies of nodC and nifH markers, were divided into two specific symbiovars, sv. phaseoli regardless of the species affiliation and sv. viciae associated with R. vallis. Through symbiotic effect assessment, all the tested strains nodulated both P. vulgaris varieties, often resulting with a significant greenness index (91-98%). However, about half of them exhibited better plant biomass performance, at least on one common bean variety, and two isolates (CYAH-6 and BLYH-15) showed a better symbiotic efficiency score. Representative strains revealed diverse abiotic stress tolerance to NaCl, acidity, alkalinity, temperature, drought and glyphosate. One strain efficient on both varieties and exhibiting stress abiotic tolerance (BLYH-15) belonged to R. genosp. IV sv. phaseoli, a species first found as a legume symbiont.

Invasive Ponto‐Caspian amphipods are less carnivorous outside their native range

AbstractA species' expansion beyond the native range is often assumed to be associated with an increased dietary niche breadth. However, empirical evidence remains limited due to a scarcity of studies comparing both the parental and invaded ranges. Here, we test the trophic niche expansion hypothesis by examining stable isotopes and functional morphology across native (NW Black Sea) and invaded (SE Baltic Sea) ranges of two amphipods, Dikerogammarus villosus and Pontogammarus robustoides, originating from the Ponto‐Caspian region—a major source of species invading Holarctic inland waters. Stable isotopes revealed that both species underwent a marked dietary niche contraction by becoming less carnivorous in the invaded range. This dietary shift was morphologically mirrored by an overall reduction of functional traits related to predation. The magnitude of dietary and morphological change was greater in D. villosus. Our findings indicate that previous experimental reports of aggressive predation in D. villosus reflect opportunistic foraging and align with local stable isotope studies which generally indicate a low trophic position. We conclude that Ponto‐Caspian species can undergo rapid dietary changes outside the native range, and that dietary niche expansion is not a prerequisite for successful non‐native establishment.

Risk assessment of the laboratory host range and a molecular characterisation determining the field host range of Lixus aemulus, for the biological control of Chromolaena odorata in South Africa

Chromolaena odorata (Asteraceae: Eupatorieae) is a sprawling shrub native to the Americas, and a destructive invader of much of the humid tropics and subtropics of the Old World. Opportunistic native-range exploration in 1995 identified a stem-boring weevil, Lixus aemulus, as a promising biological control candidate agent. Host-specificity testing was conducted on L. aemulus in South Africa using laboratory no-choice and paired-choice tests. Three invasive alien plants closely related to C. odorata may be utilized by L. aemulus but no indigenous, ornamental or crop species in South Africa was or is expected to be attacked by the weevil. A native-range field survey was conducted in Brazil to determine the exact identity of the host plant L. aemulus had been collected in 1995, and to identify additional host-plant species. Genetic assessments of the Lixus sp.(p.). adults collected on the three host plants (C. odorata, Chromolaena laevigata and Heterocondylus vitalbae) reveal these individuals are L. aemulus and the weevil can be classed as an oligophage in its native range. Over 5,500 adults were released in South Africa, but overall establishment has been poor. The most likely explanation appears to be a climate mismatch between the region of South Africa invaded by C. odorata and the collection locality in Rio Branco, Acre state, Brazil. Additionally, because the full extent of the native range of L. aemulus is unknown, it is uncertain whether individuals can be sourced from an area whose climate resembles that of South Africa. Furthermore, despite being oligophagous, L. aemulus may perform sub-optimally on the southern African C. odorata biotype.

Does introduced European Phragmites australis experience belowground microbial enemy release in North America?

AbstractEscape from native range enemies can give invasive species a competitive edge according to the enemy‐release hypothesis. While more commonly associated with predators and herbivores, release from belowground microbial antagonists has been recently demonstrated to benefit invasive plants. Biogeographic variation in dominance and comparisons of soil communities suggest that invasive European Phragmites australis may have also benefitted from belowground enemy release in North America (NA). Here we examine the effects of native range (Europe) versus introduced range (NA) soil communities on European and North American P. australis using a reciprocal inoculation seedling growth experiment. Contrary to the enemy‐release hypothesis, we found that North American P. australis was sensitive to soil community origin in that the seedlings grown in European soil communities had higher total biomass than seedlings grown in North American soil communities. This pattern was not observed in the European P. australis seedlings which had similar biomass when grown with North American or European soil communities. Notably, North American P. australis had higher biomass than European P. australis regardless of which soil community it was grown in, suggesting a growth–defense tradeoff. Though the relative abundance of mutualists and pathogens composition did not differ between the two ranges, an indicator analysis revealed that mutualistic fungi and bacteria were key components of European soil communities but not in North American communities. Interestingly, North American soil communities had lower β diversity than European communities suggesting higher levels of community conservation among North American populations. This research represents the first evidence of growth–defense trade‐offs in North American P. australis and offers a novel mechanism in understanding the invasion of P. australis in NA.

Genomic and induction evidence for bacteriophage contributions to sargassum-bacteria symbioses

BackgroundSymbioses between primary producers and bacteria are crucial for nutrient exchange that fosters host growth and niche adaptation. Yet, how viruses that infect bacteria (phages) influence these bacteria-eukaryote interactions is still largely unknown. Here, we investigate the role of viruses on the genomic diversity and functional adaptations of bacteria associated with pelagic sargassum. This brown alga has dramatically increased its distribution range in the Atlantic in the past decade and is predicted to continue expanding, imposing severe impacts on coastal ecosystems, economies, and human health.ResultsWe reconstructed 73 bacterial and 3963 viral metagenome-assembled genomes (bMAGs and vMAGs, respectively) from coastal Sargassum natans VIII and surrounding seawater. S. natans VIII bMAGs were enriched in prophages compared to seawater (28% and 0.02%, respectively). Rhodobacterales and Synechococcus bMAGs, abundant members of the S. natans VIII microbiome, were shared between the algae and seawater but were associated with distinct phages in each environment. Genes related to biofilm formation and quorum sensing were enriched in S. natans VIII phages, indicating their potential to influence algal association in their bacterial hosts. In-vitro assays with a bacterial community harvested from sargassum surface biofilms and depleted of free viruses demonstrated that these bacteria are protected from lytic infection by seawater viruses but contain intact and inducible prophages. These bacteria form thicker biofilms when growing on sargassum-supplemented seawater compared to seawater controls, and phage induction using mitomycin C was associated with a significant decrease in biofilm formation. The induced metagenomes were enriched in genomic sequences classified as temperate viruses compared to uninduced controls.ConclusionsOur data shows that prophages contribute to the flexible genomes of S. natans VIII-associated bacteria. These prophages encode genes with symbiotic functions, and their induction decreases biofilm formation, an essential capacity for flexible symbioses between bacteria and the alga. These results indicate that prophage acquisition and induction contribute to genomic and functional diversification during sargassum-bacteria symbioses, with potential implications for algae growth.1vVC6YGWLCm_DZc419o4RCVideo

Direct and indirect effects of climate and seed dynamics on the breeding performance of a seed predator at the distribution edge.

Marginal populations usually have low densities and are considered to be particularly vulnerable to environmental stochasticity. Using data collected in nest boxes, we analyzed the breeding performance of the edible dormouse (Glis glis), an obligate hibernating rodent and a seed predator in deciduous forests, in two populations at the distribution range's edge. Despite being only 20 km apart from each other, Montseny is a large patch of mixed deciduous forests (oaks and beech), whereas Montnegre would be the harshest habitat, that is, a small, isolated patch with only oaks. First, we studied the differences in climate and tree cover change in the two populations. Second, we analyzed the direct and indirect roles of local climate conditions and seed availability on breeding performance over 10 years in each population. Finally, we explored the influence of tree cover change on the occupancy dynamics in the two populations. Our results showed contrasting responses between populations: in Montseny, asynchronous seed production between oaks and beech precluded skip breeding, and breeding performance increased with seed availability. Furthermore, dormice in Montseny may use pollen production to anticipate the amount of beech nut resources and adjust their breeding effort. Boxes showed higher occupancy and colonization and fewer extinctions in Montseny than in Montnegre, where seed availability did not drive breeding performance. Results from Montnegre suggest that skip breeding was an adaptive response to a more pulsed, harsher environment. Here, females produced a similar number of pups than at Montseny. Long-term studies dealing with population responses in marginal habitats can lead to a deeper understanding of the capacities of organisms to adapt to harsh environments. Although local adaptation is frequently documented across various taxa, studies at the distribution edge may shed light on our still limited comprehension of the underlying mechanisms responsible for its occurrence.

Does ecology shape geographical parthenogenesis? Evidence from the facultatively parthenogenetic stick insect Megacrania batesii.

Closely related sexual and parthenogenetic species often show distinct distribution patterns, known as geographical parthenogenesis. Similar patterns, characterized by the existence of separate sexual and parthenogenetic populations across their natural range, can also be found in facultative parthenogens - species in which every female is capable of both sexual and parthenogenetic reproduction. The underlying mechanisms driving this phenomenon in nature remain unclear. Features of the habitat, such as differences in host-plant phenotypes or niche breadth, could favour sexual or asexual reproductive modes and thus help to explain geographical parthenogenesis in natural insect populations. Megacrania batesii is a facultatively parthenogenetic stick insect that displays geographical parthenogenesis in the wild. We aimed to explore whether sexual and parthenogenetic populations of M. batesii displayed niche differentiation or variations in niche breadth that could explain the separation of the two population types. To do this, we sampled host plants from across the range of M. batesii and quantified phenotypic traits that might affect palatability or accessibility for M. batesii, including leaf thickness, toughness, spike size and density, plant height, and chemical composition. We also quantified host-plant density, which could affect M. batesii dispersal. We found little evidence of phenotypic differences between host plants supporting sexual versus asexual M. batesii populations, and no difference in host-plant density or niche breadth between the two population types. Our results suggest that habitat parameters do not play a substantial role in shaping patterns of geographical parthenogenesis in wild populations of M. batesii. Instead, population sex ratio variation could result from interactions between the sexes or dispersal dynamics.

International Benchmark for Total Metabolic Tumor Volume Measurement in Baseline 18F-FDG PET/CT of Lymphoma Patients: A Milestone Toward Clinical Implementation.

Total metabolic tumor volume (TMTV) is prognostic in lymphoma. However, cutoff values for risk stratification vary markedly, according to the tumor delineation method used. We aimed to create a standardized TMTV benchmark datasetallowing TMTV to be tested and applied as a reproducible biomarker. Methods: Sixty baseline 18F-FDG PET/CT scans were identified with a range of disease distributions (20 follicular, 20 Hodgkin, and 20 diffuse large B-cell lymphoma). TMTV was measured by 12 nuclear medicine experts, each analyzing 20 cases split across subtypes, with each case processed by 3-4 readers. LIFEx or ACCURATE software was chosen according to reader preference. Analysis was performed stepwise: TMTV1 with automated preselection of lesions using an SUV of at least 4 and a volume of at least 3 cm3 with single-click removal of physiologic uptake; TMTV2 with additional removal of reactive bone marrow and spleen with single clicks; TMTV3 with manual editing to remove other physiologic uptake, if required; and TMTV4 with optional addition of lesions using mouse clicks with an SUV of at least 4 (no volume threshold). Results: The final TMTV (TMTV4) ranged from 8 to 2,288 cm3, showing excellent agreement among all readers in 87% of cases (52/60) with a difference of less than 10% or less than 10 cm3 In 70% of the cases, TMTV4 equaled TMTV1, requiring no additional reader interaction. Differences in the TMTV4 were exclusively related to reader interpretation of lesion inclusion or physiologic high-uptake region removal, not to the choice of software. For 5 cases, large TMTV differences (>25%) were due to disagreement about inclusion of diffuse splenic uptake. Conclusion: The proposed segmentation method enabled highly reproducible TMTV measurements, with minimal reader interaction in 70% of the patients. The inclusion or exclusion of diffuse splenic uptake requires definition of specific criteria according to lymphoma subtype. The publicly available proposed benchmark allows comparison of study results and could serve as a reference to test improvements using other segmentation approaches.

Laboratory Management of Mammalian Hosts for Ixodes scapularis -Host-Pathogen Interaction Studies.

Due to their hematophagous life cycle, hard-bodied ticks including the genus Ixodes are a potential vector for numerous pathogenic organisms including bacteria, protozoa, viruses, and infectious prions. The natural geographic range of several hard tick species, includig Ixodes scapularis, has expanded over recent decades. Consequently, there is an ongoing need to maintain, feed, and propagate ticks for host-pathogen interaction studies to better understand and mitigate their impact on human and animal health. Artificial membrane feeding of hard ticks has advanced in recent years, has study design advantages, and should be used, when possible, to reduce animal use, but it also has several limitations that require the continued use of mammalian hosts including mice, guinea pigs, and rabbits. In this overview, we discuss the best management practices for these relevant species with respect to biosafety, health, and optimal host comfort when used in studies that depend on tick feeding. The capsule-jacket method is preferred over the ear sock-E-collar method of tick feeding on rabbit hosts because of better host health, comfort, and increased study versatility.

Physiologically-informed predictions of climate warming effects on native and non-native populations of blue catfish

Blue catfish Ictalurus furcatus has been widely introduced throughout the United States to enhance recreational fisheries. Its success in both its native and non-native range, especially in the context of climate change, will be influenced by its thermal performance. We conducted a laboratory experiment to investigate the responses of wild-captured, subadult blue catfish to temperatures ranging from 7 °C to 38 °C. Blue catfish had relatively low standard metabolic rates, indicating low energetic demands, and hence an ability to survive well even during low-food conditions. Metabolic scope and food consumption rate increased with temperature, with metabolic scope peaking at 29.1 °C, and consumption rate peaking at 32 °C. Body condition remained high up to 32 °C, but decreased drastically thereafter, suggesting limitations in maintaining metabolism through food consumption at temperatures >32 °C; blue catfish cannot survive in such habitats indefinitely. Yet, many fish were able to survive temperatures as high as 38 °C for 5 days, suggesting that acute and occasionally chronic heat waves will not limit this species. Using these results, we also predicted the performance of blue catfish under prevailing conditions and under climate warming at seven locations throughout their current range in the U.S. We found that some blue catfish populations in southern and southeastern areas will likely experience temperatures above the optimal temperature for extended periods due to climate change, thus limiting potential habitat availability for this species. But, many non-native populations, especially those in northern areas such as Idaho, North Dakota, and northern California, may benefit from the expected warmer temperatures during spring and fall.

The generation mechanism of deep natural gas in Tabei uplift, Tarim Basin, Northwest China: insights from instantaneous and accumulative effects

The natural gas heavy carbon isotope and high dryness coefficients genesis in Tabei uplift, Tarim Basin has been highly controversial. To investigate the generation mechanisms of natural gas in the Tabei Uplift. Natural gas chemical composition, carbon isotopes were used to analyze the genesis of natural gas, source rock maturity, and basin modeling were conducted to reconstruct the natural gas generation process, and the influences of instantaneous and cumulative effects on natural gas properties was discussed. The results show that the dryness coefficients of natural gas range from 0.62 to 0.99 (average: 0.92), the methane contents range from 30.42% to 96.4% (average: 85.10%), ethane contents from 0.43% to 15.58% (average: 3.39%), propane contents from 0.11% to 11.43% (average: 1.78%), and the methane carbon isotopes range from −47.30‰ to −33.80‰ (average: −36.96‰), ethane carbon isotopes range from −39.60‰ to −33.20‰ (average: −35.57‰), propane carbon isotopes range from −36.90‰ to −28.50‰ (average: −35.49‰). Compared with the actual regional thermal evolution of the source rock (Ro% range from 1.4%∼1.7%), the natural gas exhibits excessively high dryness coefficients and heavy methane carbon isotope characteristics. The natural gas is primary cracking gas and sourced from marine type II kerogen. The dryness coefficient, methane carbon isotopes, and source rock maturity gradually increases from the west to the east. Instantaneous effects and leakage led to the dry gas and relative heavy methane carbon isotopes generated at a low maturity level. The current natural gas in the Ordovician reservoirs was all generated during the Himalayan orogeny. Long period pause of the gas generation between the two hydrocarbon generation phases is the main cause for the instantaneous effects.

Using Citizen Science and Field Surveys to Document the Introduction, Establishment, and Rapid Spread of the Bare-Eyed Pigeon, Patagioenas corensis, on the Island of Saint-Martin, West Indies.

Early reporting of the introduction and establishment of exotic species is of paramount importance for successful management. Here, we report the introduction and rapid spread of the Bare-eyed Pigeon, Patagioenas corensis, on the binational island of Saint-Martin, the West Indies. This range-restricted species naturally occurs in arid coastal areas of Columbia and Venezuela and nearby islands. Its introduction on Saint-Martin represents an expansion of about 1000 km beyond its established native range. Using observations recorded in eBird and results from a recent field survey, we show that since its introduction, most probably between late 2012 and early 2013, the species has expanded fast in Saint-Martin and has recently broadened its habitat to include anthropized, built areas. The expansion of Bare-eyed Pigeon on Saint-Martin and the neighboring Leeward Islands, possibly facilitated by climate change in the future, could be a threat to both native columbid species and other bird species through competition for resources. We therefore recommend that local authorities and stakeholders rapidly eradicate the species or at least prevent its further spread on Saint-Martin, possibly though listing it as a game species, while it is still possible to do so.

Combined high rates of alternative breeding strategies unexpectedly found among populations of a solitary nesting raptor.

Social monogamy is the prevalent mating system in birds, but alternative strategies of extra-pair paternity (EPP) and conspecific brood parasitism (CBP) occur in many species. Raptors are virtually absent in discussions of broad taxonomic reviews regarding EPP and CBP likely because these strategies are mostly absent or at low frequency; CBP is unreported in solitary nesting raptors. In contrast, we found high frequencies of EPP (16%-31%) and CBP (15%-26%) nests among three populations of Cooper's Hawks (Accipiter cooperii) across the northern breeding range of this solitary nesting, socially monogamous species. EPP and CBP combined occurred in 42%-46% of all nests among populations and hence unexpectedly were nearly equivalent to proportions of genetically monogamous nests. Select covariates failed to predict presence of EPP and CBP in part because virtually all extra-pair adults were uncaught and likely were floaters. We found no support for the hypothesis that territorial females traded copulations for food to maximize energy intake for increased production. Our unique discoveries enhance knowledge of the extent and diversity of alternative breeding strategies among groups of avian and other animal species.

An invasive prey and changing climate interact to shape the breeding phenology of an endangered predator.

Changes in phenology are occurring from global climate change, yet the impacts of other types of global change on the phenology of animals remain less appreciated. Understanding the potential for synergistic effects of different types of global change on phenology is needed, because changing climate regimes can have cascading effects, particularly on invasive species that vary in their thermal tolerances. Using 25 years of data from 5963 nests and 4675 marked individuals across the entire US breeding range of an endangered predator, the snail kite (Rostrhamus sociabilis plumbeus), we isolated the effects of an invasion of novel prey and warming temperatures on breeding phenology and its demographic consequences. Over this time period, breeding season length doubled, increasing by approximately 14 weeks. Both temperature and the establishment of invasive prey interacted to explain the timing of nest initiation. Temperature and invasive prey played distinct roles: earlier nest initiation occurred with increasing temperatures, whereas late nesting increased with invasion. Ultimately, both nest survival and juvenile survival declined later in the year, such that effects from invasive prey, but not warming temperatures, have the apparent potential for mistiming in breeding phenology by some individuals. Nonetheless, relatively few nesting events occurred during late fall when nest survival was very low, and seasonal declines in nest survival were weaker and renesting was more frequent in invaded wetlands, such that total reproductive output increased with invasion. Variation in demographic effects illustrate that considering only particular components of demography (e.g., nest survival rates) may be inadequate to infer the overall consequences of changes in phenology, particularly the potential for mistiming of phenological events. These results emphasize that species invasions may profoundly alter phenology of native species, such effects are distinct from climate effects, and both interact to drive population change.

From Europe to Caucasus: cryptic diversity and unexpected biogeographic history of a Western Palaearctic moss-inhabiting flea beetle (Chrysomelidae: Mniophila)

Abstract The turbulent Tertiary and Quaternary history of Europe and the Ponto-Caucasus has had a strong impact on its biota, resulting in unusual patterns of speciation, diversification, and dispersal. Our study reveals the Late-Tertiary diversification and biogeographic history of Mniophila, a Western Palaearctic endemic moss-inhabiting flightless flea beetle distributed across Europe and the Ponto-Caucasus. We assembled samples across the whole range of the genus except Crimea. Based on a molecular phylogenetic analysis, we found that the diversification of Mniophila started in the Balkan Peninsula, and was followed by at least two independent colonizations of other parts of Europe. The Ponto-Caucasian species are all descendants of a single colonization from the Balkans, followed by species’ radiation. The Mniophila beetles are, therefore, one of the first-known cases of the colonization of the Caucasus from Europe. We integrated our DNA data with morphology, allowing us to uncover the cryptic diversity of Mniophila and to revise its European species. We describe Mniophila haveli sp. nov. from the Carpathians and Balkans, M. wroblewskiiWańkowicz, 1880 from Central Europe and the Balkans is restored as a valid species, and M. bosnica Apfelbeck, 1914 is newly recognized as a Dinaric endemic. We reveal an exceedingly large distribution range for M. muscorum (Koch, 1803), which is unusual for a flightless beetle.

Mechanical performance analysis of functionally graded triply periodic minimal surfaces

To address the issue of a single gradient direction in the gradient design of triply periodic minimal surfaces, two functional gradient schemes, trapezoidal and W-shaped, were proposed based on the stress distribution range of the uniform thickness porous structure model using the primitive minimal surface porous structure. These structures were manufactured using a photosensitive resin through stereolithography additive manufacturing technology. Through finite element analysis and experimental validation, it was observed that as porosity decreases, the bending resistance of the uniform thickness model increases. The designed functional gradient porous structures were compared with the uniform thickness model. The results show that the designed gradient porous structure has a higher strength and bending stiffness than the uniform thickness porous structure. The proposed design can improve the bending performance of the porous structure. The W-shaped gradient porous structure with fine control of porosity variation range had better ability to improve structural bending performance than the trapezoidal gradient design.

Genetic diversity and population structure of an insect-pollinated and bird-dispersed dioecious tree Magnolia kwangsiensis in a fragmented karst forest landscape.

This study combined population genetics and parentage analysis to obtain foundational data for the conservation of Magnolia kwangsiensis. M. kwangsiensis is a Class I tree species that occurs in two disjunct regions in a biodiversity hotspot in southwest China. We assessed the genetic diversity and structure of this species across its distribution range to support its conservation management. Genetic diversity and population structure of 529 individuals sampled from 14 populations were investigated using seven nuclear simple sequence repeat (nSSR) markers and three chloroplast DNA (cpDNA) fragments. Parentage analysis was used to evaluate the pollen and seed dispersal distances. The nSSR marker analysis revealed a high genetic diversity in M. kwangsiensis, with an average observed (Ho) and expected heterozygosities (He) of 0.726 and 0.687, respectively. The mean and maximum pollen and seed dispersal distances were 66.4 and 95.7 m and 535.4 and 553.8 m, respectively. Our data revealed two distinct genetic groups, consistent with the disjunct geographical distribution of the M. kwangsiensis populations. Both pollen and seed dispersal movements help maintain genetic connectivity among M. kwangsiensis populations, contributing to high levels of genetic diversity. Both genetically differentiated groups corresponding to the two disjunct regions should be recognized as separate conservation units.

Radial basis function neural network and overlay sampling uniform design toward polylactic acid molecular weight prediction

Polylactic acid (PLA) is a potential polymer material as a substitute for traditional plastics, and the accurate molecular weight distribution range of PLA is strictly required in practical applications. Therefore, exploring the relationship between synthetic conditions and PLA molecular weight is crucially important. In this work, direct polycondensation combined with overlay sampling uniform design (OSUD) was applied to synthesize the low molecular weight PLA. Then a multiple regression model and two artificial neural network models on PLA molecular weight versus reaction temperature, reaction time, and catalyst dosage were developed for PLA molecular weight prediction. The characterization results indicated that the low molecular weight PLA was efficiently synthesized under this method. Meanwhile, the experimental dataset acquired from OSUD successfully established three predictive models for PLA molecular weight. Among them, both artificial neural network models had significantly better predictive performance than the regression model. Notably, the radial basis function neural network model had the best predictive accuracy with only 11.9% of mean relative error on the validation dataset, which improved by 67.7% compared with the traditional multiple regression model. This work successfully predicted PLA molecular weight in a direct polycondensation process using artificial neural network models combined with OSUD, which provided guidance for the future implementation of molecular weight-controlled polymer’ synthesis.

Prytanoides gen. nov., a new myodochine genus to include a new species from Argentina, and new records for Prytanes Distant, 1893 species (Hemiptera: Rhyparochromidae)

AbstractA new genus, Prytanoides gen. nov., is described to accommodate the new species Prytanoides prorrectus gen. et sp. nov. from Argentina. Its relationship with the presumably closely related Prytanes Distant,1893 is discussed. The distributional range of four Prytanes species is also extended: Prytanes formosus (Distant, 1882) and Prytanes foedus (Stål, 1860) are recorded for many provinces in Argentina; Prytanes oblongus (Stål, 1862) is recorded from Ecuador for the first time; and Prytanes tumens (Stål, 1874) from Mexico. For each species, we provide a colour habitus of the adult, together with photographs of the male genitalia of the new genus and species.