Moistube irrigation is an efficient method that accurately irrigates and fertilizes agricultural crops. Investigation into the mechanisms of infiltration behaviors under an adjusted working head (WKH) benefits a timely and artificially regulating moisture condition within root zones, as adapted to evapotranspiration. This study explores the laws of Moistube irrigated soil water movement under constant and adjusted working heads. Lysimeter experiments were conducted to measure Moistube irrigation cumulative infiltration, infiltration rate, and to observe wetting front area and water content distribution using digital image processing and time domain reflectometry, respectively. Treatments of constant heads (0, 1, and 2 m), increasing heads (0 to 1, 0 to 2 and 1 to 2 m) and deceasing heads (1 to 0, 2 to 0 and 2 to 1 m) were designed. The results show that (1) under constant heads, the cumulative infiltration increases linearly over time. The infiltration rate and cumulative infiltration are positively correlated with the pressure head. When WKH is increased or decreased, the infiltration rate and cumulative infiltration curves significantly change, followed by a gradual stabilization. The more the head is increased or decreased, the more evident this tendency will be. (2) When WKH is increased, the wetting front migration rate and the wetted soil moisture content marked increase; when WKH is decreased, the wetting front migration rate sharply decelerates, and the water content of the wetted soil slowly grows. They both tend to equilibrium with time. (3) By regarding the same cumulative infiltration of increased WKH and constant WKH treatments as a similar initial condition, we proposed a cumulative infiltration empirical model for Moistube irrigation under variable working head. Additionally, we treat the Moistube as a clayey porous medium and construct a HYDRUS-2D numerical model to predict the infiltration behaviors under variable WKH. The validity of the two models were well proven, with MRE and NRMSE close to 0 and NSE greater than 0.867, indicating good agreements with the experimental results. This model breaks through the limitation of constant boundary of traditional numerical model and applies variable head boundary to the boundary of the Moistube pipe, which can also effectively simulate the response mechanism of Moistube irrigation to variable WKH. The research results further confirmed the feasibility of manually adjusting the WKH to regulate the discharge of the Moistube pipe and soil moisture state. Based on the HYDRUS-2D numerical model simulation results and the root distribution and water demand of typical facility crops, the selection range of placement depth and the adjustable range of WKH of Moistube irrigation were proposed. The research results provide a theoretical reference for manual adjustment or automatic control of Moistube irrigation WKH to adapt to real-time crop water demand in agricultural production.